1
40
4
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1089/gte.2007.9994" target="_blank" rel="noreferrer noopener">http://doi.org/10.1089/gte.2007.9994</a>
Rights
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
Pages
256-268
Issue
3
Volume
11
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Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
One Multiplex Control For 29 Cystic Fibrosis Mutations
Publisher
An entity responsible for making the resource available
Genetic Testing
Date
A point or period of time associated with an event in the lifecycle of the resource
2007
2007
Subject
The topic of the resource
gene; Genetics & Heredity; Research & Experimental Medicine
Creator
An entity primarily responsible for making the resource
Lebo R V; Bixler M; Galehouse D
Description
An account of the resource
A simple approach is described to synthesize and clone an inexhaustible supply of any homozygous and/ or heterozygous controls diluted with yeast genomic DNA to mimic human genome equivalents for use throughout the entire multiplex mutation assay. As a proof of principle, the 25 cystic fibrosis mutation panel selected by the American College of Medical Genetics and four additional mutant sequences were prepared as a single control mixture. The 29 CFTR mutations were incorporated into 17 gene fragments by PCR amplification of targeted sequences using mutagenic primers on normal human genomic DNA template. Flanking primers selected to bind beyond all published PCR primer sites amplified controls for most assay platforms. The 17 synthesized 433-933-bp CFTR fragments each with one to four homozygous mutant sequences were cloned into nine plasmid vectors at the multiple cloning site and bidirectionally sequenced. Miniplasmid preps from these nine clones were mixed and diluted with genomic yeast DNA to mimic the final nucleotide molar ratio of two CFTR genes in 6 x 10(9) bp total human genomic DNA. This mixture was added to control PCR reactions prior to amplification as the only positive control sample. In this fashion <200 multiplex clinical PCR analyses of > 4,000 clinical patient samples have been controlled simultaneously for PCR amplification and substrate specificity for 29 tested mutations without cross contamination. This clinically validated multiplex cystic fibrosis control can be modified readily for different test formats and provides a robust means to control for all mutations instead of rotating human genomic controls each with a fraction of the mutations. This approach allows scores of additional mutation controls from any gene loci to be added to the same mixture annually.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1089/gte.2007.9994" target="_blank" rel="noreferrer noopener">10.1089/gte.2007.9994</a>
Format
The file format, physical medium, or dimensions of the resource
Journal Article or Conference Abstract Publication
2007
Bixler M
Galehouse D
gene
Genetic Testing
Genetics & Heredity
Journal Article or Conference Abstract Publication
Lebo R V
Research & Experimental Medicine
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
n/a
Rights
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
Pages
414-414
Issue
5
Volume
73
Search for Full-text
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Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Multiple Synthesized Control Mutations Optimize Clinical Test Quality
Publisher
An entity responsible for making the resource available
American Journal of Human Genetics
Date
A point or period of time associated with an event in the lifecycle of the resource
2003
2003-11
Subject
The topic of the resource
Genetics & Heredity
Creator
An entity primarily responsible for making the resource
Lebo R; Dunphy G; Quicci M; Galehouse D
Identifier
An unambiguous reference to the resource within a given context
n/a
Format
The file format, physical medium, or dimensions of the resource
Journal Article or Conference Abstract Publication
2003
American Journal of Human Genetics
Dunphy G
Galehouse D
Genetics & Heredity
Journal Article or Conference Abstract Publication
Lebo R
Quicci M
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1200/jco.2014.32.26_suppl.31" target="_blank" rel="noreferrer noopener">http://doi.org/10.1200/jco.2014.32.26_suppl.31</a>
Rights
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
Pages
1-1
Issue
26
Volume
32
Search for Full-text
Locate full-text within NEOMED Library's e-journal collections
<p>Users with a NEOMED Library login can search for full-text journal articles at the following url: <a href="https://libraryguides.neomed.edu/home">https://libraryguides.neomed.edu/home</a></p>
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
HER2 Delta 16 expression in HER2-positive breast cancer
Publisher
An entity responsible for making the resource available
Journal of Clinical Oncology
Date
A point or period of time associated with an event in the lifecycle of the resource
2014
2014-09
Subject
The topic of the resource
Oncology
Creator
An entity primarily responsible for making the resource
De Yao J T; Sun D Y; Galehouse D; Shorten S; Haller N A; Rehmus E H
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1200/jco.2014.32.26_suppl.31" target="_blank" rel="noreferrer noopener">10.1200/jco.2014.32.26_suppl.31</a>
Format
The file format, physical medium, or dimensions of the resource
Journal Article or Conference Abstract Publication
2014
De Yao J T
Department of Internal Medicine
Galehouse D
Haller N A
Journal Article or Conference Abstract Publication
Journal of Clinical Oncology
NEOMED College of Medicine
oncology
Rehmus E H
Shorten S
Sun D Y
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1016/0042-6822(92)90093-5" target="_blank" rel="noreferrer noopener">http://doi.org/10.1016/0042-6822(92)90093-5</a>
Pages
339–341
Issue
1
Volume
186
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Differences in the integration pattern and episomal forms of human papillomavirus type 16 DNA found within an invasive cervical neoplasm and its metastasis.
Publisher
An entity responsible for making the resource available
Virology
Date
A point or period of time associated with an event in the lifecycle of the resource
1992
1992-01
Subject
The topic of the resource
Carcinoma; DNA; Electrophoresis; Female; Gel; Humans; Neoplasm Metastasis; Papillomaviridae/*genetics; Plasmids; Squamous Cell/*microbiology/pathology; Two-Dimensional; Uterine Cervical Neoplasms/*microbiology/pathology; Vaginal Neoplasms/microbiology/pathology/secondary; Viral/analysis; Virus Integration
Creator
An entity primarily responsible for making the resource
Galehouse D; Jenison E; DeLucia A
Description
An account of the resource
Human papillomavirus (HPV) type 16 DNA was found in three separate neoplastic lesions within a female patient. The physical state of the viral DNA in each lesion was determined by two-dimensional agarose gel electrophoresis. The primary cervical tumor contained large amounts of several distinct episomal forms as well as integrated HPV DNA. Metastatic tumor tissue found in the vagina had greatly reduced levels of episomal DNA and a viral DNA integration pattern that was different from that of the primary tumor. The vulvar carcinoma in situ had what appears to be free and integrated forms of viral DNA. The results show that although metastatic tissue retained HPV DNA, further rearrangements of the integrated viral DNA pattern found in the primary tumor may occur with a dramatic decrease of episomal forms during malignant progression.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1016/0042-6822(92)90093-5" target="_blank" rel="noreferrer noopener">10.1016/0042-6822(92)90093-5</a>
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
1992
Carcinoma
DeLucia A
Department of Integrative Medical Sciences
DNA
Electrophoresis
Female
Galehouse D
Gel
Humans
Jenison E
NEOMED College of Medicine
Neoplasm Metastasis
Papillomaviridae/*genetics
Plasmids
Squamous Cell/*microbiology/pathology
Two-Dimensional
Uterine Cervical Neoplasms/*microbiology/pathology
Vaginal Neoplasms/microbiology/pathology/secondary
Viral/analysis
Virology
Virus Integration