1
40
16
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1002/ptr.5799" target="_blank" rel="noreferrer noopener">http://doi.org/10.1002/ptr.5799</a>
Pages
778–782
Issue
5
Volume
31
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
A Polyphenol-rich Pomegranate Fruit Extract Suppresses NF-kappaB and IL-6 Expression by Blocking the Activation of IKKbeta and NIK in Primary Human Chondrocytes.
Publisher
An entity responsible for making the resource available
Phytotherapy research : PTR
Date
A point or period of time associated with an event in the lifecycle of the resource
2017
2017-05
Subject
The topic of the resource
chondrocytes; Chondrocytes – Drug Effects; Chondrocytes/drug effects; Fruit; Fruit/chemistry; Gene Expression Regulation/drug effects; Genes – Drug Effects; Humans; I-kappa B Kinase/genetics/*metabolism; IkappaB; IKKbeta; IL-1beta; Interleukin 1 – Metabolism; Interleukin-1beta/metabolism; Interleukin-6/genetics/*metabolism; Interleukins; Interleukins – Metabolism; NF-kappa B; NF-kappa B – Metabolism; NF-kappa B/genetics/*metabolism; NF-kappaB; NIK; osteoarthritis; Phosphorylation – Drug Effects; Phosphorylation/drug effects; Plant Extracts; Plant Extracts – Pharmacodynamics; Plant Extracts/chemistry/*pharmacology; Polyphenols – Pharmacodynamics; Polyphenols/pharmacology; pomegranate; Pomegranate; Protein-Serine-Threonine Kinases/genetics/*metabolism; Proteins – Metabolism; Punicaceae/*chemistry; Signal Transduction – Drug Effects; Signal Transduction/drug effects; Transcription Factor RelA/metabolism; Transferases; Transferases – Metabolism
Creator
An entity primarily responsible for making the resource
Haseeb Abdul; Khan Nazir M; Ashruf Omer S; Haqqi Tariq M
Description
An account of the resource
Pomegranate fruit extract (PE) rich in polyphenols has been shown to exert chondroprotective effects, but the mechanism is not established. Here, we used an in vitro model of inflammation in osteoarthritis (OA) to investigate the potential of PE to suppress interleukin 1 beta (IL-1beta)-stimulated expression of inflammatory cytokine IL-6, generation of reactive oxygen species (ROS) levels, and investigated the mechanism of NF-kappaB inhibition by analyzing the activation of the kinases upstream of IkappaBalpha in primary human chondrocytes. Total and phosphorylated forms of kinases and expression of IL-6 were determined at protein and mRNA levels by western immunoblotting and Taqman assay, respectively. Dihydrorhodamine 123 staining estimated ROS generation. Pomegranate fruit extract inhibited the mRNA and protein expression of IL-6, generation of ROS, and inhibited the IL-1beta-mediated phosphorylation of inhibitor of nuclear factor kappa-B kinase subunit beta (IKKbeta), expression of IKKbeta mRNA, degradation of IkappaBalpha, and activation and nuclear translocation of
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1002/ptr.5799" target="_blank" rel="noreferrer noopener">10.1002/ptr.5799</a>
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
2017
Ashruf Omer S
Chondrocytes
Chondrocytes – Drug Effects
Chondrocytes/drug effects
Department of Anatomy & Neurobiology
Fruit
Fruit/chemistry
Gene Expression Regulation/drug effects
Genes – Drug Effects
Haqqi Tariq M
Haseeb Abdul
Humans
I-kappa B Kinase/genetics/*metabolism
IkappaB
IKKbeta
IL-1beta
Interleukin 1 – Metabolism
Interleukin-1beta/metabolism
Interleukin-6/genetics/*metabolism
Interleukins
Interleukins – Metabolism
Khan Nazir M
NEOMED College of Medicine
NF-kappa B
NF-kappa B – Metabolism
NF-kappa B/genetics/*metabolism
NF-kappaB
NIK
Osteoarthritis
Phosphorylation – Drug Effects
Phosphorylation/drug effects
Phytotherapy research : PTR
Plant Extracts
Plant Extracts – Pharmacodynamics
Plant Extracts/chemistry/*pharmacology
Polyphenols – Pharmacodynamics
Polyphenols/pharmacology
Pomegranate
Protein-Serine-Threonine Kinases/genetics/*metabolism
Proteins – Metabolism
Punicaceae/*chemistry
Signal Transduction – Drug Effects
Signal Transduction/drug effects
Transcription Factor RelA/metabolism
Transferases
Transferases – Metabolism
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1007/s00705-002-0912-5" target="_blank" rel="noreferrer noopener">http://doi.org/10.1007/s00705-002-0912-5</a>
Pages
329–344
Issue
2
Volume
148
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Multiple mechanisms for HSV-1 induction of interferon alpha production by peripheral blood mononuclear cells.
Publisher
An entity responsible for making the resource available
Archives of virology
Date
A point or period of time associated with an event in the lifecycle of the resource
2003
2003-02
Subject
The topic of the resource
Fucose/pharmacology; Gene Expression Regulation/drug effects; Herpesvirus 1; Human/classification/drug effects/genetics/*physiology; Humans; Interferon-alpha/*biosynthesis; Mutation; Myeloid Cells/drug effects/*metabolism/*virology; Species Specificity; Virus Replication
Creator
An entity primarily responsible for making the resource
Rong Q; Alexander T S; Koski G K; Rosenthal K S
Description
An account of the resource
UV-inactivated, infectious, and other forms of herpes simplex virus 1 (HSV-1) induced interferon (IFN) production by different routes in myeloid origin mononuclear cells (MOMC) (consisting predominantly of monocytes). GM-CSF activated the MOMC (G-MOMC) to produce greater amounts of interferon while differentiation to DC, by the addition of granulocyte macrophage colony stimulating factor (GM-CSF) and calcium ionophore (GA-MOMC), reduced the levels of interferon production upon challenge with some HSV strains. UV-inactivated virus induced more interferon than infectious virus. L-fucose, an antagonist of the mannose receptor, inhibited the induction of IFN-alpha by UV-inactivated virus and gB(-) virus (defective in penetration) in MOMC and GA-MOMC but not
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1007/s00705-002-0912-5" target="_blank" rel="noreferrer noopener">10.1007/s00705-002-0912-5</a>
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
2003
Alexander T S
Archives of virology
Fucose/pharmacology
Gene Expression Regulation/drug effects
Herpesvirus 1
Human/classification/drug effects/genetics/*physiology
Humans
Interferon-alpha/*biosynthesis
Koski G K
Mutation
Myeloid Cells/drug effects/*metabolism/*virology
Rong Q
Rosenthal K S
Species Specificity
Virus Replication
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1016/0169-328x(93)90070-6" target="_blank" rel="noreferrer noopener">http://doi.org/10.1016/0169-328x(93)90070-6</a>
Pages
36–40
Issue
1
Volume
17
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Para-chlorophenylalanine treatment inhibits the expression of vasoactive intestinal peptide messenger RNA in rat anterior pituitary.
Publisher
An entity responsible for making the resource available
Brain research. Molecular brain research
Date
A point or period of time associated with an event in the lifecycle of the resource
1993
1993-01
Subject
The topic of the resource
alpha-Methyltyrosine; Animals; Anterior/*drug effects/metabolism; Chemical; Depression; Dopamine/metabolism; Fenclonine/*pharmacology; Gene Expression Regulation/drug effects; Male; Messenger/*biosynthesis; Methyltyrosines/pharmacology; Pituitary Gland; Prolactin/metabolism; Rats; RNA; Serotonin/metabolism; Sprague-Dawley; Tryptophan Hydroxylase/antagonists & inhibitors; Vasoactive Intestinal Peptide/*biosynthesis/genetics
Creator
An entity primarily responsible for making the resource
Signs S A; Dluzen D E; Carrillo A J
Description
An account of the resource
Adult male Sprague-Dawley rats were treated with para-chlorophenylalanine (pCPA) or alpha-methyl tyrosine (alpha-MT) to study the effect of serotonin or catecholamine depletion on the expression of vasoactive intestinal peptide (VIP) messenger RNA in the anterior pituitary. Single injections of pCPA (300 mg/kg) for two consecutive days resulted on the third day in a dramatic depletion of serotonin in the medial basal hypothalamus, and a significant reduction in the pituitary content of VIP mRNA (1.0 and 1.7 kb). The effect of pCPA on VIP mRNA appeared to be relatively specific for the anterior pituitary since VIP message levels in the cerebral cortex did not decrease. alpha-MT treatment, (150 mg/kg) for 2 consecutive days, reduced dopamine concentrations in the MBH but had no significant effect on pituitary VIP levels. In a time-course study, hypothalamic serotonin and pituitary VIP mRNA levels were significantly depressed 1-3 days after initiation of pCPA treatment; however, 12 days after pCPA treatment, serotonin concentrations in the hypothalamus approached control values and pituitary VIP mRNA content increased an average of 2-fold over control levels in an apparent rebound effect. pCPA-treated rats injected i.p. twice a day with
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1016/0169-328x(93)90070-6" target="_blank" rel="noreferrer noopener">10.1016/0169-328x(93)90070-6</a>
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
1993
alpha-Methyltyrosine
Animals
Anterior/*drug effects/metabolism
Brain research. Molecular brain research
Carrillo A J
Chemical
Depression
Dluzen D E
Dopamine/metabolism
Fenclonine/*pharmacology
Gene Expression Regulation/drug effects
Male
Messenger/*biosynthesis
Methyltyrosines/pharmacology
Pituitary Gland
Prolactin/metabolism
Rats
RNA
Serotonin/metabolism
Signs S A
Sprague-Dawley
Tryptophan Hydroxylase/antagonists & inhibitors
Vasoactive Intestinal Peptide/*biosynthesis/genetics
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1016/j.freeradbiomed.2017.02.041" target="_blank" rel="noreferrer noopener">http://doi.org/10.1016/j.freeradbiomed.2017.02.041</a>
Pages
288–301
Volume
106
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Wogonin, a plant derived small molecule, exerts potent anti-inflammatory and chondroprotective effects through the activation of ROS/ERK/Nrf2 signaling pathways in human Osteoarthritis chondrocytes.
Publisher
An entity responsible for making the resource available
Free radical biology & medicine
Date
A point or period of time associated with an event in the lifecycle of the resource
2017
2017-05
Subject
The topic of the resource
*ERK1/2; *Nrf2; *Osteoarthritis; *Redox; *Wogonin; Anti-Inflammatory Agents/administration & dosage; Chondrocytes/drug effects/pathology; Flavanones/*administration & dosage; Gene Expression Regulation/drug effects; Humans; Inflammation/*drug therapy/metabolism/pathology; Kelch-Like ECH-Associated Protein 1/chemistry/*genetics/metabolism; MAP Kinase Signaling System/drug effects; Molecular Docking Simulation; NF-E2-Related Factor 2/antagonists & inhibitors/chemistry/*genetics/metabolism; Osteoarthritis/*drug therapy/metabolism/pathology; Protein Binding; Reactive Oxygen Species/metabolism; Signal Transduction/drug effects
Creator
An entity primarily responsible for making the resource
Khan Nazir M; Haseeb Abdul; Ansari Mohammad Y; Devarapalli Pratap; Haynie Sara; Haqqi Tariq M
Description
An account of the resource
Osteoarthritis (OA), characterized by progressive destruction of articular cartilage, is the most common form of human arthritis. Here, we evaluated the potential chondroprotective and anti-inflammatory effects of Wogonin, a naturally occurring flavonoid, in IL-1beta-stimulated human OA chondrocytes and cartilage explants. Wogonin completely suppressed the expression and production of inflammatory mediators including IL-6, COX-2, PGE2, iNOS and NO in
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1016/j.freeradbiomed.2017.02.041" target="_blank" rel="noreferrer noopener">10.1016/j.freeradbiomed.2017.02.041</a>
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Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
*ERK1/2
*Nrf2
*OSTEOARTHRITIS
*Redox
*Wogonin
2017
Ansari Mohammad Y
Anti-Inflammatory Agents/administration & dosage
Chondrocytes/drug effects/pathology
Department of Anatomy & Neurobiology
Devarapalli Pratap
Flavanones/*administration & dosage
Free radical biology & medicine
Gene Expression Regulation/drug effects
Haqqi Tariq M
Haseeb Abdul
Haynie Sara
Humans
Inflammation/*drug therapy/metabolism/pathology
Kelch-Like ECH-Associated Protein 1/chemistry/*genetics/metabolism
Khan Nazir M
MAP Kinase Signaling System/drug effects
Molecular Docking Simulation
NEOMED College of Medicine
NF-E2-Related Factor 2/antagonists & inhibitors/chemistry/*genetics/metabolism
Osteoarthritis/*drug therapy/metabolism/pathology
Protein Binding
Reactive Oxygen Species/metabolism
Signal Transduction/drug effects
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1016/j.molimm.2005.12.003" target="_blank" rel="noreferrer noopener">http://doi.org/10.1016/j.molimm.2005.12.003</a>
Pages
1933–1940
Issue
12
Volume
43
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Interleukin IL-12 blocks a specific subset of the transcriptional profile responsive to UVB in epidermal keratinocytes.
Publisher
An entity responsible for making the resource available
Molecular immunology
Date
A point or period of time associated with an event in the lifecycle of the resource
2006
2006-05
Subject
The topic of the resource
*Keratinocytes/metabolism/radiation effects; *Ultraviolet Rays; Cell Culture Techniques; Cells; Cultured; Epidermal Cells; Gene Expression Regulation/drug effects; Genetic/*radiation effects; Humans; Interleukin-12/*genetics/*metabolism/pharmacology; Transcription
Creator
An entity primarily responsible for making the resource
Molenda Matthew; Mukkamala Lakshmi; Blumenberg Miroslav
Description
An account of the resource
Interleukin-12 (IL-12) is a proinflammatory and immunomodulatory cytokine that plays a critical role it in innate and adaptive immunity by inducing production of interferon-gamma and other cytokines. IL-12 was shown to block the ultraviolet light-induced immunosuppression, important in cancer immunosurveillance, cutaneous allergies and inflammation. To characterize the molecular effects of
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1016/j.molimm.2005.12.003" target="_blank" rel="noreferrer noopener">10.1016/j.molimm.2005.12.003</a>
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
*Keratinocytes/metabolism/radiation effects
*Ultraviolet Rays
2006
Blumenberg Miroslav
Cell Culture Techniques
Cells
Cultured
Epidermal Cells
Gene Expression Regulation/drug effects
Genetic/*radiation effects
Humans
Interleukin-12/*genetics/*metabolism/pharmacology
Molecular immunology
Molenda Matthew
Mukkamala Lakshmi
Transcription
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1038/onc.2017.140" target="_blank" rel="noreferrer noopener">http://doi.org/10.1038/onc.2017.140</a>
Pages
5189–5198
Issue
36
Volume
36
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Thrombospondin-4 mediates TGF-beta-induced angiogenesis.
Publisher
An entity responsible for making the resource available
Oncogene
Date
A point or period of time associated with an event in the lifecycle of the resource
2017
2017-09
Subject
The topic of the resource
Angiogenesis Inducing Agents/*metabolism; Animals; Cell Movement/drug effects; Cell Proliferation/drug effects; Cells; Chick Embryo; Cultured; Endothelium; Female; Gene Expression Regulation/drug effects; Humans; Inbred C57BL; Male; Mice; Muscle; Neovascularization; Pathologic/drug therapy/metabolism/*pathology; Signal Transduction/drug effects; Smooth; Thrombospondins/*physiology; Transforming Growth Factor beta/*pharmacology; Vascular/drug effects/metabolism/*pathology
Creator
An entity primarily responsible for making the resource
Muppala S; Xiao R; Krukovets I; Verbovetsky D; Yendamuri R; Habib N; Raman P; Plow E; Stenina-Adognravi O
Description
An account of the resource
TGF-beta is a multifunctional cytokine affecting many cell types and implicated in tissue remodeling processes. Due to its many functions and cell-specific effects, the consequences of TGF-beta signaling are process-and stage-dependent, and it is not uncommon that TGF-beta exerts distinct and sometimes opposing effects on a disease progression depending on the stage and on the pathological changes associated with the stage. The mechanisms underlying cell- and process-specific effects of TGF-beta are poorly understood. We are describing a novel pathway that mediates induction of angiogenesis in response to TGF-beta1. We found that in endothelial cells (EC) thrombospondin-4 (TSP-4), a secreted extracellular matrix (ECM) protein, is upregulated in response to TGF-beta1 and mediates the effects of TGF-beta1 on angiogenesis. Upregulation of TSP-4 does not require the synthesis of new protein, is not caused by decreased secretion of
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1038/onc.2017.140" target="_blank" rel="noreferrer noopener">10.1038/onc.2017.140</a>
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
2017
Angiogenesis Inducing Agents/*metabolism
Animals
Cell Movement/drug effects
Cell Proliferation/drug effects
Cells
Chick Embryo
Cultured
Department of Integrative Medical Sciences
Endothelium
Female
Gene Expression Regulation/drug effects
Habib N
Humans
Inbred C57BL
Krukovets I
Male
Mice
Muppala S
Muscle
NEOMED College of Medicine
Neovascularization
Oncogene
Pathologic/drug therapy/metabolism/*pathology
Plow E
Raman P
Signal Transduction/drug effects
Smooth
Stenina-Adognravi O
Thrombospondins/*physiology
Transforming Growth Factor beta/*pharmacology
Vascular/drug effects/metabolism/*pathology
Verbovetsky D
Xiao R
Yendamuri R
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1038/srep42843" target="_blank" rel="noreferrer noopener">http://doi.org/10.1038/srep42843</a>
Pages
42843–42843
Volume
7
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Mechanisms of Chinese Medicine Xinmailong's protection against heart failure in pressure-overloaded mice and cultured cardiomyocytes.
Publisher
An entity responsible for making the resource available
Scientific reports
Date
A point or period of time associated with an event in the lifecycle of the resource
2017
2017-02
Subject
The topic of the resource
Animal; Animals; Cardiac/cytology/*drug effects/metabolism; Cell Nucleus/metabolism; Cell Survival/drug effects; Cells; Chinese Herbal/*administration & dosage/pharmacology; Constriction; Cultured; Disease Models; Drugs; Echocardiography; GABA Plasma Membrane Transport Proteins/*metabolism; Gene Expression Regulation/drug effects; Glycogen Synthase Kinase 3 beta/metabolism; Heart Failure/etiology/metabolism/physiopathology/*prevention & control; Humans; MAP Kinase Signaling System/drug effects; Mice; Myocytes; Pathologic; Phosphorylation; Proto-Oncogene Proteins c-akt/metabolism; Rats
Creator
An entity primarily responsible for making the resource
Qi Jianyong; Yu Juan; Tan Yafang; Chen Renshan; Xu Wen; Chen Yanfen; Lu Jun; Liu Qin; Wu Jiashin; Gu Weiwang; Zhang Minzhou
Description
An account of the resource
Patients with heart failure (HF) have high mortality and mobility. Xinmailong (XML) injection, a Chinese Medicine, is clinically effective in treating HF. However, the mechanism of XML's effectiveness on HF was unclear, and thus, was the target of the present study. We created a mouse model of pressure-overload-induced HF with transverse aortic constriction (TAC) surgery and compared among 4 study groups: SHAM (n = 10), TAC (n = 12), MET (metoprolol, positive drug treatment, n = 7) and XML (XML treatment, n = 14). Dynamic changes in cardiac structure and function were evaluated with echocardiography in vivo. In addition, H9C2 rat cardiomyocytes were cultured in vitro and the phosphorylation of ERK1/2, AKT, GSK3beta and protein expression of GATA4 in nucleus were detected with Western blot experiment. The results showed that XML reduced diastolic thickness of left ventricular posterior wall, increased ejection fraction and fraction shortening, so as to inhibit HF at 2 weeks after TAC. Moreover, XML inhibited the phosphorylation of ERK1/2, AKT and GSK3beta, subsequently inhibiting protein expression of GATA4 in nucleus (P \textless 0.001). Together, our data demonstrated that XML inhibited the TAC-induced HF via inactivating the ERK1/2, AKT/GSK3beta, and GATA4 signaling pathway.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1038/srep42843" target="_blank" rel="noreferrer noopener">10.1038/srep42843</a>
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
2017
Animal
Animals
Cardiac/cytology/*drug effects/metabolism
Cell Nucleus/metabolism
Cell Survival/drug effects
Cells
Chen Renshan
Chen Yanfen
Chinese Herbal/*administration & dosage/pharmacology
Constriction
Cultured
Disease Models
Drugs
Echocardiography
GABA Plasma Membrane Transport Proteins/*metabolism
Gene Expression Regulation/drug effects
Glycogen Synthase Kinase 3 beta/metabolism
Gu Weiwang
Heart Failure/etiology/metabolism/physiopathology/*prevention & control
Humans
Liu Qin
Lu Jun
MAP Kinase Signaling System/drug effects
Mice
Myocytes
Pathologic
Phosphorylation
Proto-Oncogene Proteins c-akt/metabolism
Qi Jianyong
Rats
Scientific reports
Tan Yafang
Wu Jiashin
Xu Wen
Yu Juan
Zhang Minzhou
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1038/srep43789" target="_blank" rel="noreferrer noopener">http://doi.org/10.1038/srep43789</a>
Pages
43789–43789
Volume
7
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
A wogonin-rich-fraction of Scutellaria baicalensis root extract exerts chondroprotective effects by suppressing IL-1beta-induced activation of AP-1 in human OA chondrocytes.
Publisher
An entity responsible for making the resource available
Scientific reports
Date
A point or period of time associated with an event in the lifecycle of the resource
2017
2017-03
Subject
The topic of the resource
Cells; Chemical Fractionation/methods; Chondrocytes/*drug effects/metabolism; Cultured; Cyclooxygenase 2/genetics/metabolism; Flavanones/*pharmacology; Gene Expression Regulation/drug effects; Humans; Interleukin-1beta/pharmacology; Interleukin-6/genetics/metabolism; Osteoarthritis/pathology; Plant Extracts/*pharmacology; Plant Roots/*chemistry; Protective Agents/pharmacology; Reactive Oxygen Species/metabolism; Scutellaria baicalensis/*chemistry; Transcription Factor AP-1/genetics/*metabolism
Creator
An entity primarily responsible for making the resource
Khan Nazir M; Haseeb Abdul; Ansari Mohammad Y; Haqqi Tariq M
Description
An account of the resource
Osteoarthritis (OA) is a common joint disorder with varying degrees of inflammation and sustained oxidative stress. The root extract of Scutellaria baicalensis (SBE) has been used for the treatment of inflammatory and other diseases. Here, we performed activity-guided HPLC-fractionation of SBE, identified the active ingredient(s) and investigated its chondroprotective potential. We found that the Wogonin containing fraction-4 (F4) was the most potent fraction based on its ability to inhibit ROS production and the suppression of catabolic markers including IL-6, COX-2, iNOS, MMP-3, MMP-9,
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1038/srep43789" target="_blank" rel="noreferrer noopener">10.1038/srep43789</a>
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
2017
Ansari Mohammad Y
Cells
Chemical Fractionation/methods
Chondrocytes/*drug effects/metabolism
Cultured
Cyclooxygenase 2/genetics/metabolism
Department of Anatomy & Neurobiology
Flavanones/*pharmacology
Gene Expression Regulation/drug effects
Haqqi Tariq M
Haseeb Abdul
Humans
Interleukin-1beta/pharmacology
Interleukin-6/genetics/metabolism
Khan Nazir M
NEOMED College of Medicine
Osteoarthritis/pathology
Plant Extracts/*pharmacology
Plant Roots/*chemistry
Protective Agents/pharmacology
Reactive Oxygen Species/metabolism
Scientific reports
Scutellaria baicalensis/*chemistry
Transcription Factor AP-1/genetics/*metabolism
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1089/ten.TEA.2009.0078" target="_blank" rel="noreferrer noopener">http://doi.org/10.1089/ten.TEA.2009.0078</a>
Pages
3765–3778
Issue
12
Volume
15
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Development of bone and cartilage in tissue-engineered human middle phalanx models.
Publisher
An entity responsible for making the resource available
Tissue engineering. Part A
Date
A point or period of time associated with an event in the lifecycle of the resource
2009
2009-12
Subject
The topic of the resource
*Models; Aggrecans/genetics/metabolism; Animals; Biological; Bone Development/drug effects/*physiology; Calcium Phosphates/pharmacology; Cartilage/cytology/drug effects/*growth & development; Cattle; Chondrocytes/cytology/drug effects/metabolism; Collagen Type II/genetics/metabolism; Durapatite/pharmacology; Electron; Experimental; Finger Phalanges/cytology/diagnostic imaging/drug effects/*physiology; Gene Expression Profiling; Gene Expression Regulation/drug effects; Humans; Implants; Integrin-Binding Sialoprotein; Mice; Microscopy; Paraffin Embedding; Periosteum/cytology/drug effects; Polyesters/pharmacology; Radiography; Scanning; Sialoglycoproteins/genetics/metabolism; Tissue Engineering/*methods; Tissue Scaffolds/chemistry
Creator
An entity primarily responsible for making the resource
Wada Yoshitaka; Enjo Mitsuhiro; Isogai Noritaka; Jacquet Robin; Lowder Elizabeth; Landis William J
Description
An account of the resource
Human middle phalanges were tissue-engineered with midshaft scaffolds of poly(L-lactide-epsilon-caprolactone) [P(LA-CL)], hydroxyapatite-P(LA-CL), or beta-tricalcium phosphate-P(LA-CL) and end plate scaffolds of bovine chondrocyte-seeded polyglycolic acid. Midshafts were either wrapped with bovine periosteum or left uncovered. Constructs implanted in nude mice for up to 20 weeks were examined for cartilage and bone development as well as gene expression and protein secretion, which are important in extracellular matrix (ECM) formation and mineralization. Harvested 10- and 20-week constructs without periosteum maintained end plate cartilage but no growth plate formation. They also consisted of chondrocytes secreting type II collagen and proteoglycan, and they were composed of midshaft regions devoid of bone. In all periosteum-wrapped constructs at like times, end plate scaffolds held chondrocytes elaborating type II collagen and proteoglycan and cartilage growth plates resembling normal tissue. Chondrocyte gene expression of type II collagen, aggrecan, and bone sialoprotein varied depending on midshaft composition, presence of periosteum, and length of implantation time. Periosteum produced additional cells, ECM, and mineral formation within the different midshaft scaffolds. Periosteum thus induces midshaft development and mediates chondrocyte gene expression and growth plate formation in cartilage regions of phalanges. This work is important for understanding developmental principles of tissue-engineered phalanges and by extension those of normal growth plate cartilage and bone.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1089/ten.TEA.2009.0078" target="_blank" rel="noreferrer noopener">10.1089/ten.TEA.2009.0078</a>
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
*Models
2009
Aggrecans/genetics/metabolism
Animals
Biological
Bone Development/drug effects/*physiology
Calcium Phosphates/pharmacology
Cartilage/cytology/drug effects/*growth & development
Cattle
Chondrocytes/cytology/drug effects/metabolism
Collagen Type II/genetics/metabolism
Durapatite/pharmacology
Electron
Enjo Mitsuhiro
Experimental
Finger Phalanges/cytology/diagnostic imaging/drug effects/*physiology
Gene Expression Profiling
Gene Expression Regulation/drug effects
Humans
Implants
Integrin-Binding Sialoprotein
Isogai Noritaka
Jacquet Robin
Landis William J
Lowder Elizabeth
Mice
Microscopy
Paraffin Embedding
Periosteum/cytology/drug effects
Polyesters/pharmacology
Radiography
Scanning
Sialoglycoproteins/genetics/metabolism
Tissue engineering. Part A
Tissue Engineering/*methods
Tissue Scaffolds/chemistry
Wada Yoshitaka
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1152/ajplung.90629.2008" target="_blank" rel="noreferrer noopener">http://doi.org/10.1152/ajplung.90629.2008</a>
Pages
L487–495
Issue
3
Volume
297
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Beta-adrenoceptor stimulation of alveolar fluid clearance is increased in rats with heart failure.
Publisher
An entity responsible for making the resource available
American journal of physiology. Lung cellular and molecular physiology
Date
A point or period of time associated with an event in the lifecycle of the resource
2009
2009-09
Subject
The topic of the resource
Adrenergic; Animals; beta/*metabolism; Body Fluids/*metabolism; Epithelial Cells/drug effects/metabolism/pathology; Gene Expression Regulation/drug effects; Heart Failure/blood/diagnostic imaging/*metabolism/*pathology; Hormones/blood; Hyperplasia; Ion Channels/genetics/metabolism; Male; Messenger/genetics/metabolism; Myocardial Infarction/blood/diagnostic imaging/pathology; Pulmonary Alveoli/drug effects/*pathology; Rats; Receptors; RNA; Sprague-Dawley; Terbutaline/pharmacology; Ultrasonography
Creator
An entity primarily responsible for making the resource
Maron Michael B; Luther Daniel J; Pilati Charles F; Ohanyan Vahagn; Li Tianbo; Koshy Shyny; Horne Walter I; Meszaros J Gary; Walro Jon M; Folkesson Hans G
Description
An account of the resource
The alveolar epithelium plays a critical role in resolving pulmonary edema. We thus hypothesized that its function might be upregulated in rats with heart failure, a condition that severely challenges the lung's ability to maintain fluid balance. Heart failure was induced by left coronary artery ligation. Echocardiographic and cardiovascular hemodynamics confirmed its development at 16 wk postligation. At that time, alveolar fluid clearance was measured by an increase in protein concentration over 1 h of a 5% albumin solution instilled into the lungs. Baseline alveolar fluid clearance was similar in heart failure and age-matched control rats. Terbutaline was added to the instillate to determine whether heart failure rats responded to beta-adrenoceptor stimulation. Alveolar fluid clearance in heart failure rats was increased by 194% after terbutaline stimulation compared with a 153% increase by terbutaline in control rats. To determine the mechanisms responsible for this accelerated alveolar fluid clearance, we measured ion transporter expression (ENaC, Na-K- ATPase, CFTR). No significant upregulation was observed for these ion transporters in the heart failure rats. Lung morphology showed significant alveolar epithelial type II cell hyperplasia in heart failure rats. Thus, alveolar epithelial type II cell hyperplasia is the likely explanation for the increased terbutaline-stimulated alveolar fluid clearance in heart failure rats. These data provide evidence for previously unrecognized mechanisms that can protect against or hasten resolution of alveolar edema in heart failure.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1152/ajplung.90629.2008" target="_blank" rel="noreferrer noopener">10.1152/ajplung.90629.2008</a>
Rights
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Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
2009
Adrenergic
American journal of physiology. Lung cellular and molecular physiology
Animals
beta/*metabolism
Body Fluids/*metabolism
Department of Integrative Medical Sciences
Epithelial Cells/drug effects/metabolism/pathology
Folkesson Hans G
Gene Expression Regulation/drug effects
Heart Failure/blood/diagnostic imaging/*metabolism/*pathology
Hormones/blood
Horne Walter I
Hyperplasia
Ion Channels/genetics/metabolism
Koshy Shyny
Li Tianbo
Luther Daniel J
Male
Maron Michael B
Messenger/genetics/metabolism
Meszaros J Gary
Myocardial Infarction/blood/diagnostic imaging/pathology
NEOMED College of Medicine
Ohanyan Vahagn
Pilati Charles F
Pulmonary Alveoli/drug effects/*pathology
Rats
Receptors
RNA
Sprague-Dawley
Terbutaline/pharmacology
Ultrasonography
Walro Jon M
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1186/1465-9921-9-55" target="_blank" rel="noreferrer noopener">http://doi.org/10.1186/1465-9921-9-55</a>
Pages
55–55
Volume
9
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
RNA interference for CFTR attenuates lung fluid absorption at birth in rats.
Publisher
An entity responsible for making the resource available
Respiratory research
Date
A point or period of time associated with an event in the lifecycle of the resource
2008
2008-07
Subject
The topic of the resource
*RNA Interference; Animals; Biological Transport/drug effects; Cells; Cultured; Cystic Fibrosis Transmembrane Conductance Regulator/*genetics/*metabolism; Epithelial Sodium Channels/genetics/metabolism; Female; Gene Expression Regulation/drug effects; Lung/cytology/drug effects/*metabolism; Messenger/metabolism; Newborn; Pregnancy; Rats; Respiratory Mucosa/cytology/drug effects/metabolism; RNA; Small Interfering/*pharmacology; Sprague-Dawley; Water/*metabolism
Creator
An entity primarily responsible for making the resource
Li Tianbo; Koshy Shyny; Folkesson Hans G
Description
An account of the resource
BACKGROUND: Small interfering RNA (siRNA) against alphaENaC (alpha-subunit of the epithelial Na channel) and CFTR (cystic fibrosis transmembrane conductance regulator) was used to explore ENaC and CTFR function in newborn rat lungs. METHODS: Twenty-four hours after trans-thoracic intrapulmonary (ttip) injection of siRNA-generating plasmid DNA (pSi-0, pSi-4, or pSi-C2), we measured CFTR and ENaC expression, extravascular lung water, and mortality. RESULTS: alphaENaC and CFTR mRNA and protein decreased by approximately 80% and approximately 85%, respectively, following alphaENaC and CFTR silencing. Extravascular lung water and mortality increased after alphaENaC and CFTR-silencing. In pSi-C2-transfected isolated DLE cells there were attenuated CFTR mRNA and protein. In pSi-4-transfected DLE cells alphaENaC mRNA and protein were both reduced. Interestingly, CFTR-silencing also reduced alphaENaC mRNA and protein. alphaENaC silencing, on the other hand, only slightly reduced CFTR mRNA and protein. CONCLUSION: Thus, ENaC and CFTR are both involved in the fluid secretion to absorption conversion around at birth.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1186/1465-9921-9-55" target="_blank" rel="noreferrer noopener">10.1186/1465-9921-9-55</a>
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Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
*RNA Interference
2008
Animals
Biological Transport/drug effects
Cells
Cultured
Cystic Fibrosis Transmembrane Conductance Regulator/*genetics/*metabolism
Epithelial Sodium Channels/genetics/metabolism
Female
Folkesson Hans G
Gene Expression Regulation/drug effects
Koshy Shyny
Li Tianbo
Lung/cytology/drug effects/*metabolism
Messenger/metabolism
Newborn
Pregnancy
Rats
Respiratory Mucosa/cytology/drug effects/metabolism
Respiratory research
RNA
Small Interfering/*pharmacology
Sprague-Dawley
Water/*metabolism
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1186/s12929-015-0133-3" target="_blank" rel="noreferrer noopener">http://doi.org/10.1186/s12929-015-0133-3</a>
Pages
30–30
Volume
22
Dublin Core
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Title
A name given to the resource
The orphan nuclear receptor small heterodimer partner is required for thiazolidinedione effects in leptin-deficient mice.
Publisher
An entity responsible for making the resource available
Journal of biomedical science
Date
A point or period of time associated with an event in the lifecycle of the resource
2015
2015-05
Subject
The topic of the resource
Animals; Bile Acids and Salts/metabolism; Cytoplasmic and Nuclear/biosynthesis/*genetics/metabolism; Diabetes Mellitus/*drug therapy/genetics/metabolism; Gene Expression Regulation/drug effects; Glucose/metabolism; Hepatocytes/drug effects; Humans; Insulin Resistance/genetics; Insulin/*metabolism; Leptin/deficiency/genetics; Lipid Metabolism/drug effects; Messenger/genetics; Mice; Obese; PPAR gamma/*biosynthesis/genetics; Receptors; RNA; Thiazolidinediones/*administration & dosage
Creator
An entity primarily responsible for making the resource
Tseng Hsiu-Ting; Park Young Joo; Lee Yoon-Kwang; Moore David D
Description
An account of the resource
BACKGROUND: Small heterodimer partner (SHP, NR0B2) is involved in diverse metabolic pathways, including hepatic bile acid, lipid and glucose homeostasis, and has been implicated in effects on the peroxisome proliferator-activated receptor gamma (PPARgamma), a master regulator of adipogenesis and the receptor for antidiabetic drugs thiazolidinediones (TZDs). In this study, we aim to investigate the role of SHP in TZD response by comparing TZD-treated leptin-deficient (ob/ob) and leptin-, SHP-deficient (ob/ob;Shp(-/-)) double mutant mice. RESULTS: Both ob/ob and double mutant ob/ob;Shp(-/-) mice developed hyperglycemia, insulin resistance, and hyperlipidemia, but hepatic fat accumulation was decreased in the double mutant ob/ob;Shp(-/-) mice. PPARgamma2 mRNA levels were markedly lower in ob/ob;Shp(-/-) liver and decreased to a lesser extent in adipose tissue. The TZD troglitazone did not reduce glucose or circulating triglyceride levels in ob/ob;Shp(-/-) mice. Expression of the adipocytokines, such as adiponectin and resistin, was not stimulated by troglitazone treatment. Expression of hepatic lipogenic genes was also reduced in ob/ob;Shp(-/-) mice. Moreover, overexpression of SHP by adenovirus infection increased PPARgamma2 mRNA levels in mouse primary hepatocytes. CONCLUSIONS: Our results suggest that SHP is required for both antidiabetic and hypolipidemic effects of TZDs in ob/ob mice through regulation of PPARgamma expression.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1186/s12929-015-0133-3" target="_blank" rel="noreferrer noopener">10.1186/s12929-015-0133-3</a>
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
2015
Animals
Bile Acids and Salts/metabolism
Cytoplasmic and Nuclear/biosynthesis/*genetics/metabolism
Department of Integrative Medical Sciences
Diabetes Mellitus/*drug therapy/genetics/metabolism
Gene Expression Regulation/drug effects
Glucose/metabolism
Hepatocytes/drug effects
Humans
Insulin Resistance/genetics
Insulin/*metabolism
Journal of biomedical science
Lee Yoon-Kwang
Leptin/deficiency/genetics
Lipid Metabolism/drug effects
Messenger/genetics
Mice
Moore David D
NEOMED College of Medicine
Obese
Park Young Joo
PPAR gamma/*biosynthesis/genetics
Receptors
RNA
Thiazolidinediones/*administration & dosage
Tseng Hsiu-Ting
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.2174/138920112798868575" target="_blank" rel="noreferrer noopener">http://doi.org/10.2174/138920112798868575</a>
Pages
229–234
Issue
1
Volume
13
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Alteration of hepatic proinflammatory cytokines is involved in the resveratrol-mediated chemoprevention of chemically-induced hepatocarcinogenesis.
Publisher
An entity responsible for making the resource available
Current pharmaceutical biotechnology
Date
A point or period of time associated with an event in the lifecycle of the resource
2012
2012-01
Subject
The topic of the resource
Female; Animals; Rats; Gene Expression Regulation/drug effects; Liver/drug effects/metabolism; Resveratrol; Diethylnitrosamine; Anticarcinogenic Agents/pharmacology/*therapeutic use; Cytokines/genetics/*metabolism; Phenobarbital; Stilbenes/pharmacology/*therapeutic use; Sprague-Dawley; RNA; Messenger/metabolism; Liver Neoplasms; Experimental/chemically induced/metabolism/*prevention & control
Creator
An entity primarily responsible for making the resource
Mbimba Thomas; Awale Prabha; Bhatia Deepak; Geldenhuys Werner J; Darvesh Altaf S; Carroll Richard T; Bishayee Anupam
Description
An account of the resource
Hepatocellular carcinoma (HCC), one of the most common cancers in the world, is a leading cause of cancerrelated mortality. HCC develops most frequently in the background of oxidative stress and chronic hepatic inflammation due to viral infections, alcohol abuse as well as exposure to environmental and dietary carcinogens. As the prognosis of HCC is extremely poor and mostly unresponsive to current chemotherapeutic treatment regimens, novel preventive approaches like chemoprevention are urgently needed. We have recently found that resveratrol, a dietary polyphenol present in grapes, berries, peanuts as well as red wine, prevents diethylnitrosamine (DENA)-initiated hepatocarcinogenesis in rats through suppression of inflammation and oxidative stress. As cytokines are considered to be important mediators of inflammation, the objective of the present study was to investigate the effects of resveratrol on hepatic cytokines during DENA-initiated hepatocarcinogenesis in rats. Liver samples were harvested from our previous study in which resveratrol (50, 100 and 300 mg/kg) was found to exert a chemopreventive action against rat liver tumorigenesis induced by DENA. The levels of proinflammatory cytokines, namely tumor necrosis factor-alpha (TNF-alpha), interleukin-1beta (IL-1beta) and interleukin- 6 (IL-6), were measured using enzyme-linked immunosorbent assays. The mRNA expression of these cytokines was studied by reverse transcriptase-polymerase chain reaction for comparison. Resveratrol treatment reversed the DENAinduced alteration of the level and expression of hepatic TNF-alpha, IL-1beta and IL-6. From the current results in conjunction with our previous findings, it can be concluded that resveratrol-mediated chemoprevention of rat liver carcinogenesis is related to alteration of proinflammatory cytokines.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.2174/138920112798868575" target="_blank" rel="noreferrer noopener">10.2174/138920112798868575</a>
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
2012
Animals
Anticarcinogenic Agents/pharmacology/*therapeutic use
Awale Prabha
Bhatia Deepak
Bishayee Anupam
Carroll Richard T
Current pharmaceutical biotechnology
Cytokines/genetics/*metabolism
Darvesh Altaf S
Department of Pharmaceutical Sciences
Diethylnitrosamine
Experimental/chemically induced/metabolism/*prevention & control
Female
Geldenhuys Werner J
Gene Expression Regulation/drug effects
Liver Neoplasms
Liver/drug effects/metabolism
Mbimba Thomas
Messenger/metabolism
NEOMED College of Pharmacy
Phenobarbital
Rats
Resveratrol
RNA
Sprague-Dawley
Stilbenes/pharmacology/*therapeutic use
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
Pages
71–82
Volume
313
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Regulation of human sterol 27-hydroxylase gene (CYP27A1) by bile acids and hepatocyte nuclear factor 4alpha (HNF4alpha).
Publisher
An entity responsible for making the resource available
Gene
Date
A point or period of time associated with an event in the lifecycle of the resource
2003
2003-08
Subject
The topic of the resource
Humans; Cell Line; Transfection; Gene Expression Regulation/drug effects; Base Sequence; Binding Sites/genetics; Response Elements/genetics; Molecular Sequence Data; Mutation; Chenodeoxycholic Acid/pharmacology; Transcription Factors/genetics/*metabolism; Hepatocyte Nuclear Factor 4; Mutagenesis; *DNA-Binding Proteins; Basic Helix-Loop-Helix Leucine Zipper Transcription Factors; Bile Acids and Salts/*pharmacology; Cholestanetriol 26-Monooxygenase; DNA/chemistry/genetics; Luciferases/genetics/metabolism; Phosphoproteins/genetics/*metabolism; Recombinant Fusion Proteins/genetics/metabolism; Steroid Hydroxylases/*genetics; DNA; Dose-Response Relationship; Drug; Cultured; Receptors; Tumor Cells; Cloning; Molecular; Sequence Analysis; Promoter Regions; Genetic/*genetics; Cytoplasmic and Nuclear/genetics/metabolism; Site-Directed
Creator
An entity primarily responsible for making the resource
Chen Wenling; Chiang John Y L
Description
An account of the resource
Mitochondrial sterol 27-hydroxylase (CYP27A1) catalyses sterol side-chain oxidation of bile acid synthesis from cholesterol, and the first reaction of the acidic bile acid biosynthetic pathway. Hydrophobic bile acids suppress human CYP27A1 gene reporter activity when assayed in human hepatocellular blastoma HepG2 cells. Bile acids also inhibit CYP27A1 reporter activity in human embryonic kidney 293 cells. A putative bile acid response element (BARE) was mapped to a region downstream of nt -147 of the human CYP27A1 gene, within which a binding site for a liver-specific nuclear receptor, HNF4alpha, is identified. HNF4alpha strongly stimulates CYP27A1 gene transcription and mutation of its binding site markedly reduced promoter activity. Results suggest that human CYP27A1 gene transcription is suppressed by bile acids and HNF4alpha plays a pivotal role in transcriptional regulation of this gene.
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
*DNA-Binding Proteins
2003
Base Sequence
Basic Helix-Loop-Helix Leucine Zipper Transcription Factors
Bile Acids and Salts/*pharmacology
Binding Sites/genetics
Cell Line
Chen Wenling
Chenodeoxycholic Acid/pharmacology
Chiang John Y L
Cholestanetriol 26-Monooxygenase
Cloning
Cultured
Cytoplasmic and Nuclear/genetics/metabolism
Department of Integrative Medical Sciences
DNA
DNA/chemistry/genetics
Dose-Response Relationship
Drug
gene
Gene Expression Regulation/drug effects
Genetic/*genetics
Hepatocyte Nuclear Factor 4
Humans
Luciferases/genetics/metabolism
Molecular
Molecular Sequence Data
Mutagenesis
Mutation
NEOMED College of Medicine
Phosphoproteins/genetics/*metabolism
Promoter Regions
Receptors
Recombinant Fusion Proteins/genetics/metabolism
Response Elements/genetics
Sequence Analysis
Site-Directed
Steroid Hydroxylases/*genetics
Transcription Factors/genetics/*metabolism
Transfection
Tumor Cells
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
Pages
257–265
Issue
1
Volume
262
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Regulation of cholesterol 7alpha-hydroxylase gene (CYP7A1) transcription by the liver orphan receptor (LXRalpha).
Publisher
An entity responsible for making the resource available
Gene
Date
A point or period of time associated with an event in the lifecycle of the resource
2001
2001-01
Subject
The topic of the resource
Humans; Animals; Binding Sites; Rats; Species Specificity; Transfection; Gene Expression Regulation/drug effects; Organ Specificity; Transcription Factors/genetics/metabolism; Cricetinae; Response Elements; Luciferases/genetics/metabolism; Retinoid X Receptors; Cholesterol 7-alpha-Hydroxylase/drug effects/*genetics/metabolism; Hydroxycholesterols; Liver/physiology; Lovastatin/pharmacology; Mevalonic Acid/metabolism/pharmacology; Nicotinic Acids/pharmacology; Polyisoprenyl Phosphates/pharmacology; Tetrahydronaphthalenes/pharmacology; Cells; Cultured; Receptors; Transcription; Genetic; Retinoic Acid/genetics/metabolism; Steroid/genetics/*metabolism
Creator
An entity primarily responsible for making the resource
Chiang J Y; Kimmel R; Stroup D
Description
An account of the resource
The cholesterol 7alpha-hydroxylase gene (CYP7A1) plays an important role in regulation of bile acid biosynthesis and cholesterol homeostasis. Oxysterol receptor, LXR, stimulates, whereas the bile acid receptor, FXR, inhibits CYP7A1 transcription. The goal of this study was to investigate the role of LXRalpha on the regulation of rat, human and hamster CYP7A1 transcription in its native promoter and cellular context. Cotransfection with LXRalpha and RXRalpha expression plasmids strongly stimulated rat CYP7A1/luciferase reporter activity in HepG2 cells and oxysterol was not required. However, LXRalpha had much less effect on hamster and no significant effect on human CYP7A1 promoter activity in HepG2 cells. In Chinese hamster ovary cells, cotransfection with LXRalpha stimulated reporter activity by less than 2-fold and addition of 22(R)-hydroxycholesterol caused a small but significant stimulation of rat, human and hamster CYP7A1 promoter activity. At least two direct repeats of AGGTCA-like sequences with 4-base spacing (DR4) and five-base spacing (DR5), in previously identified bile acid response elements of the rat CYP7A1 were able to bind LXRalpha/RXRalpha and confer LXRalpha stimulation. However, LXRalpha did not bind to the corresponding sequences of the human gene and bound weakly to hamster and mouse DR4 sequences. Therefore, rats and mice have the unusual capacity to convert cholesterol to bile acids by LXRalpha-mediated stimulation of CYP7A1 transcription, whereas other species do not respond to cholesterol and develop hypercholesterolemia on a diet high in cholesterol.
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
2001
Animals
Binding Sites
Cells
Chiang J Y
Cholesterol 7-alpha-Hydroxylase/drug effects/*genetics/metabolism
Cricetinae
Cultured
Department of Integrative Medical Sciences
gene
Gene Expression Regulation/drug effects
Genetic
Humans
Hydroxycholesterols
Kimmel R
Liver/physiology
Lovastatin/pharmacology
Luciferases/genetics/metabolism
Mevalonic Acid/metabolism/pharmacology
NEOMED College of Medicine
Nicotinic Acids/pharmacology
Organ Specificity
Polyisoprenyl Phosphates/pharmacology
Rats
Receptors
Response Elements
Retinoic Acid/genetics/metabolism
Retinoid X Receptors
Species Specificity
Steroid/genetics/*metabolism
Stroup D
Tetrahydronaphthalenes/pharmacology
Transcription
Transcription Factors/genetics/metabolism
Transfection
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
Pages
191–195
Issue
1
Volume
304
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Guggulsterone antagonizes farnesoid X receptor induction of bile salt export pump but activates pregnane X receptor to inhibit cholesterol 7alpha-hydroxylase gene.
Publisher
An entity responsible for making the resource available
Biochemical and biophysical research communications
Date
A point or period of time associated with an event in the lifecycle of the resource
2003
2003-04
Subject
The topic of the resource
Humans; Cell Line; Gene Expression Regulation/drug effects; Pregnane X Receptor; Cholesterol 7-alpha-Hydroxylase/*genetics; ATP-Binding Cassette Transporters/*genetics; Chenodeoxycholic Acid/antagonists & inhibitors; DNA-Binding Proteins/*antagonists & inhibitors/metabolism; Pregnenediones/*pharmacology; Transcription Factors/*antagonists & inhibitors/metabolism; Transcriptional Activation/drug effects; Dose-Response Relationship; Drug; Receptors; ATP Binding Cassette Transporter; Subfamily B; Cytoplasmic and Nuclear/*metabolism; Steroid/*metabolism; Member 11
Creator
An entity primarily responsible for making the resource
Owsley Erika; Chiang John Y L
Description
An account of the resource
Bile acids activate a nuclear receptor, farnesoid X receptor (FXR), that induces bile salt export pump (BSEP) but inhibits cholesterol 7alpha-hydroxylase (CYP7A1) gene transcription in the liver. Guggulsterone, a plant sterol that lowers serum cholesterol, has been shown to antagonize FXR activated genes. Transient transfection assay of a human BSEP/luciferase reporter in HepG2 cells transfected with FXR reveals that guggulsterone strongly antagonizes bile acid induction of the BSEP gene. On the other hand, guggulsterone has no effect on FXR inhibition of the CYP7A1 gene, but strongly inhibits the human CYP7A1 gene by activation of pregnane X receptor (PXR). These results suggest that guggulsterone inhibits bile acid secretion from hepatocytes into bile and activates PXR to inhibit bile acid synthesis in the liver. Reduced conversion of cholesterol and bile acid excretion may lead to an increase of hepatic cholesterol and decrease of intestinal cholesterol absorption, and results in lowering serum cholesterol.
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Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
2003
ATP Binding Cassette Transporter
ATP-Binding Cassette Transporters/*genetics
Biochemical and biophysical research communications
Cell Line
Chenodeoxycholic Acid/antagonists & inhibitors
Chiang John Y L
Cholesterol 7-alpha-Hydroxylase/*genetics
Cytoplasmic and Nuclear/*metabolism
Department of Integrative Medical Sciences
DNA-Binding Proteins/*antagonists & inhibitors/metabolism
Dose-Response Relationship
Drug
Gene Expression Regulation/drug effects
Humans
Member 11
NEOMED College of Medicine
Owsley Erika
Pregnane X Receptor
Pregnenediones/*pharmacology
Receptors
Steroid/*metabolism
Subfamily B
Transcription Factors/*antagonists & inhibitors/metabolism
Transcriptional Activation/drug effects