1
40
2
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Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1016/0006-291x(91)91776-9" target="_blank" rel="noreferrer noopener">http://doi.org/10.1016/0006-291x(91)91776-9</a>
Pages
38–44
Issue
1
Volume
178
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
The human liver and reticulocyte cytochrome b5 mRNAs are products from a single gene.
Publisher
An entity responsible for making the resource available
Biochemical and biophysical research communications
Date
A point or period of time associated with an event in the lifecycle of the resource
1991
1991-07
Subject
The topic of the resource
*Genes; Amino Acid Sequence; Base Sequence; Cytochromes b5/blood/*genetics; Gene Library; Genetic; Humans; Liver/*metabolism; Molecular Sequence Data; Polymerase Chain Reaction; Polymorphism; Reticulocytes/*metabolism; RNA/blood/genetics/isolation & purification
Creator
An entity primarily responsible for making the resource
Giordano S J; Steggles A W
Description
An account of the resource
Using a combination of standard cDNA library screening techniques and the Polymerase Chain Reaction (PCR) we have isolated and sequenced DNA fragments corresponding to the human reticulocyte cytochrome b5 mRNA. The reticulocyte specific sequence codes for amino acids 97 and 98 only, with a TAA stop codon. The reticulocyte specific 3'non-translated sequence has 15 new nucleotides then utilizes the liver mRNA sequence from amino acid 97 onward. This indicates that the reticulocyte specific exon has 24 base pairs (bp). In addition, we have isolated sequences that are derived from a transcribed cytochrome b5 pseudogene. This transcript contains multiple mutations which prevent the synthesis of any functional protein.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1016/0006-291x(91)91776-9" target="_blank" rel="noreferrer noopener">10.1016/0006-291x(91)91776-9</a>
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
*Genes
1991
Amino Acid Sequence
Base Sequence
Biochemical and biophysical research communications
Cytochromes b5/blood/*genetics
Gene Library
Genetic
Giordano S J
Humans
Liver/*metabolism
Molecular Sequence Data
Polymerase Chain Reaction
Polymorphism
Reticulocytes/*metabolism
RNA/blood/genetics/isolation & purification
Steggles A W
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1006/bbrc.1995.1582" target="_blank" rel="noreferrer noopener">http://doi.org/10.1006/bbrc.1995.1582</a>
Pages
894–900
Issue
3
Volume
209
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
The isolation and characterization of the human cytochrome b5 gene.
Publisher
An entity responsible for making the resource available
Biochemical and biophysical research communications
Date
A point or period of time associated with an event in the lifecycle of the resource
1995
1995-04
Subject
The topic of the resource
Animals; Base Sequence; Cattle; Cell Line; Cloning; Cosmids; Cultured; Cytochromes b5/biosynthesis/*genetics; DNA Primers; DNA/genetics/isolation & purification; Gene Library; Genetic; Hominidae/*genetics; Humans; Introns; Luciferases/biosynthesis; Messenger/analysis/biosynthesis; Molecular; Molecular Sequence Data; Polymerase Chain Reaction; Promoter Regions; Rabbits; RNA; Transfection; Tumor Cells
Creator
An entity primarily responsible for making the resource
Li X R; Giordano S J; Yoo M; Steggles A W
Description
An account of the resource
From a series of lambda and cosmid libraries, we isolated DNA sequences corresponding to the complete coding region for the human cytochrome b5 (CYB5) gene. The overall gene organization was the same as the bovine and rabbit CYB5 genes. One cosmid clone containing exon I plus the 5' flanking region was extensively characterized. From this clone we obtained 2000 bp of 5' flanking sequence, containing several distinctive GC rich regions, potential trans-acting factor binding sites and a 74 bp direct repeat. A series of deletion constructs were made in the pGL2 luciferase vector and then used to transfect HepG2 and K562 cells. The data obtained suggest the presence of two promotors and one silencer region in the analyzed 5' sequence.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1006/bbrc.1995.1582" target="_blank" rel="noreferrer noopener">10.1006/bbrc.1995.1582</a>
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
1995
Animals
Base Sequence
Biochemical and biophysical research communications
Cattle
Cell Line
Cloning
Cosmids
Cultured
Cytochromes b5/biosynthesis/*genetics
DNA Primers
DNA/genetics/isolation & purification
Gene Library
Genetic
Giordano S J
Hominidae/*genetics
Humans
Introns
Li X R
Luciferases/biosynthesis
Messenger/analysis/biosynthesis
Molecular
Molecular Sequence Data
Polymerase Chain Reaction
Promoter Regions
Rabbits
RNA
Steggles A W
Transfection
Tumor Cells
Yoo M