1
40
8
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1210/me.2009-0482" target="_blank" rel="noreferrer noopener">http://doi.org/10.1210/me.2009-0482</a>
Pages
1151–1164
Issue
6
Volume
24
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
A novel bile acid-activated vitamin D receptor signaling in human hepatocytes.
Publisher
An entity responsible for making the resource available
Molecular endocrinology (Baltimore, Md.)
Date
A point or period of time associated with an event in the lifecycle of the resource
2010
2010-06
Subject
The topic of the resource
Calcitriol/*metabolism; Calcitriol/pharmacology; Cell Membrane/drug effects/metabolism; Cell Nucleus/drug effects/metabolism; Cholesterol 7-alpha-Hydroxylase/antagonists & inhibitors/genetics; Enzyme Activation/drug effects; Extracellular Signal-Regulated MAP Kinases/antagonists & inhibitors; Genetic/genetics; Hep G2 Cells; Hepatocyte Nuclear Factor 4/metabolism; Hepatocytes/*drug effects/enzymology/*metabolism; Humans; Intracellular Space/drug effects/metabolism; Ligands; Lithocholic Acid/*pharmacology; Mitogen-Activated Protein Kinase Kinases/metabolism; Phosphorylation/drug effects; Phosphotyrosine/metabolism; Promoter Regions; Protein Kinase Inhibitors/pharmacology; Protein Transport/drug effects; Proto-Oncogene Proteins c-raf/metabolism; Receptors; Retinoid X Receptor alpha/metabolism; Signal Transduction/*drug effects; src-Family Kinases/metabolism; Steroid Hydroxylases/genetics/metabolism; Vitamin D3 24-Hydroxylase
Creator
An entity primarily responsible for making the resource
Han Shuxin; Li Tiangang; Ellis Ewa; Strom Stephen; Chiang John Y L
Description
An account of the resource
Vitamin D receptor (VDR) is activated by natural ligands, 1alpha,
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1210/me.2009-0482" target="_blank" rel="noreferrer noopener">10.1210/me.2009-0482</a>
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
2010
Calcitriol/*metabolism
Calcitriol/pharmacology
Cell Membrane/drug effects/metabolism
Cell Nucleus/drug effects/metabolism
Chiang John Y L
Cholesterol 7-alpha-Hydroxylase/antagonists & inhibitors/genetics
Department of Integrative Medical Sciences
Ellis Ewa
Enzyme Activation/drug effects
Extracellular Signal-Regulated MAP Kinases/antagonists & inhibitors
Genetic/genetics
Han Shuxin
Hep G2 Cells
Hepatocyte Nuclear Factor 4/metabolism
Hepatocytes/*drug effects/enzymology/*metabolism
Humans
Intracellular Space/drug effects/metabolism
Li Tiangang
Ligands
Lithocholic Acid/*pharmacology
Mitogen-Activated Protein Kinase Kinases/metabolism
Molecular endocrinology (Baltimore, Md.)
NEOMED College of Medicine
Phosphorylation/drug effects
Phosphotyrosine/metabolism
Promoter Regions
Protein Kinase Inhibitors/pharmacology
Protein Transport/drug effects
Proto-Oncogene Proteins c-raf/metabolism
Receptors
Retinoid X Receptor alpha/metabolism
Signal Transduction/*drug effects
src-Family Kinases/metabolism
Steroid Hydroxylases/genetics/metabolism
Strom Stephen
Vitamin D3 24-Hydroxylase
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1016/j.molbrainres.2005.02.030" target="_blank" rel="noreferrer noopener">http://doi.org/10.1016/j.molbrainres.2005.02.030</a>
Pages
143–151
Issue
1
Volume
137
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
APeg3, a novel paternally expressed gene 3 antisense RNA transcript specifically expressed in vasopressinergic magnocellular neurons in the rat supraoptic nucleus.
Publisher
An entity responsible for making the resource available
Brain research. Molecular brain research
Date
A point or period of time associated with an event in the lifecycle of the resource
2005
2005-06
Subject
The topic of the resource
3' Untranslated Regions/genetics; Amino Acid; Animals; Antisense/*genetics/isolation & purification/*metabolism; Conserved Sequence/genetics; DNA-Binding Proteins/genetics/*metabolism; Gene Expression Regulation/*genetics; Genetic/genetics; Genomic Imprinting/genetics; Hypothalamo-Hypophyseal System/cytology/metabolism; Kruppel-Like Transcription Factors; Male; Messenger/genetics/metabolism; Molecular Sequence Data; Neurons/*metabolism; Nucleic Acid; Protein Kinases/genetics/*metabolism; Rats; RNA; Sequence Homology; Sprague-Dawley; Supraoptic Nucleus/cytology/*metabolism; Transcription; Transcription Factors/genetics/*metabolism; Vasopressins/*metabolism; Water-Electrolyte Balance/genetics
Creator
An entity primarily responsible for making the resource
Glasgow Eric; Ryu Seung-Lim; Yamashita Mitsuo; Zhang Bing-Jun; Mutsuga Noriko; Gainer Harold
Description
An account of the resource
Vasopressin (VP) and oxytocin (OT) play critical roles in the regulation of salt and water balance, lactation, and various behaviors and are expressed at very high levels in specific magnocellular neurons (MCNs) in the hypothalamo-neurohypophysial system (HNS). In addition to the cell-specific expression of the VP and OT genes in these cells, there are other transcripts that are preferentially expressed in the VP or OT MCNs. One such gene, paternally expressed gene 3 (Peg3), is an imprinted gene expressed exclusively from the paternal allele that encodes a Kruppel-type zinc finger-containing protein involved in maternal behavior and is abundantly expressed in the VP-MCNs. We report here the robust expression in the VP-MCNs of an RNA, which we designate APeg3 that is transcribed in the antisense direction to the 3' untranslated region of the Peg3 gene. The APeg3 mRNA is about 1 kb in size, and the full-length sequence of APeg3, as determined by 5' and 3' RACE, contains an open reading frame that predicts a protein of 93 amino acids and is predominantly expressed in VP-MCNs. Both Peg3 and APeg3 gene expression in the VP-MCNs increase during systemic hyperosmolality in vivo, demonstrating that both of these genes are osmoregulated.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1016/j.molbrainres.2005.02.030" target="_blank" rel="noreferrer noopener">10.1016/j.molbrainres.2005.02.030</a>
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
2005
3' Untranslated Regions/genetics
Amino Acid
Animals
Antisense/*genetics/isolation & purification/*metabolism
Brain research. Molecular brain research
Conserved Sequence/genetics
DNA-Binding Proteins/genetics/*metabolism
Gainer Harold
Gene Expression Regulation/*genetics
Genetic/genetics
Genomic Imprinting/genetics
Glasgow Eric
Hypothalamo-Hypophyseal System/cytology/metabolism
Kruppel-Like Transcription Factors
Male
Messenger/genetics/metabolism
Molecular Sequence Data
Mutsuga Noriko
Neurons/*metabolism
Nucleic Acid
Protein Kinases/genetics/*metabolism
Rats
RNA
Ryu Seung-Lim
Sequence Homology
Sprague-Dawley
Supraoptic Nucleus/cytology/*metabolism
Transcription
Transcription Factors/genetics/*metabolism
Vasopressins/*metabolism
Water-Electrolyte Balance/genetics
Yamashita Mitsuo
Zhang Bing-Jun
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1006/bbrc.1994.1080" target="_blank" rel="noreferrer noopener">http://doi.org/10.1006/bbrc.1994.1080</a>
Pages
546–553
Issue
2
Volume
198
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Effects of bile acids and steroid/thyroid hormones on the expression of cholesterol 7 alpha-hydroxylase mRNA and the CYP7 gene in HepG2 cells.
Publisher
An entity responsible for making the resource available
Biochemical and biophysical research communications
Date
A point or period of time associated with an event in the lifecycle of the resource
1994
1994-01
Subject
The topic of the resource
Animals; Bile Acids and Salts/*pharmacology; Cells; Cholesterol 7-alpha-Hydroxylase/*genetics; Cultured; Enzymologic/*drug effects; Gene Expression Regulation; Genetic; Genetic/genetics; Humans; Luciferases/biosynthesis/genetics; Messenger/*biosynthesis; Promoter Regions; Rats; Recombinant Fusion Proteins/biosynthesis; RNA; Steroids/*pharmacology; Thyroid Hormones/*pharmacology; Transcription; Transfection
Creator
An entity primarily responsible for making the resource
Crestani M; Karam W G; Chiang J Y
Description
An account of the resource
The expression of cholesterol 7 alpha-hydroxylase mRNA levels in confluent HepG2 cultures was reduced by tauro- or glyco-conjugates of deoxycholate and chenodeoxycholate, but not by cholate. Ursodeoxycholates, on the other hand, stimulated the mRNA level. The 5'-upstream regions of rat cholesterol 7 alpha-hydroxylase gene (CYP7) were fused to luciferase reporter gene and the constructs, p-3616/Luc, p-224/Luc and p-160/Luc, were transiently transfected into HepG2 cells. Tauro-conjugates of deoxycholate and chenodeoxycholate inhibited the transcriptional activities of the gene constructs in the confluent cells, but not in subconfluent cells. These results reveal that bile acid responsive elements are located in the -160 fragment and also between nt -3616 and -224. Thyroid and steroid hormones stimulated transcriptional activity expressed in the confluent cells and their responsive elements are located upstream of nt -224. It appears that adult phenotypes are responsible for bile acid feedback and hormone response in HepG2 cells.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1006/bbrc.1994.1080" target="_blank" rel="noreferrer noopener">10.1006/bbrc.1994.1080</a>
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
1994
Animals
Bile Acids and Salts/*pharmacology
Biochemical and biophysical research communications
Cells
Chiang J Y
Cholesterol 7-alpha-Hydroxylase/*genetics
Crestani M
Cultured
Department of Integrative Medical Sciences
Enzymologic/*drug effects
Gene Expression Regulation
Genetic
Genetic/genetics
Humans
Karam W G
Luciferases/biosynthesis/genetics
Messenger/*biosynthesis
NEOMED College of Medicine
Promoter Regions
Rats
Recombinant Fusion Proteins/biosynthesis
RNA
Steroids/*pharmacology
Thyroid Hormones/*pharmacology
Transcription
Transfection
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1074/jbc.M111.305789" target="_blank" rel="noreferrer noopener">http://doi.org/10.1074/jbc.M111.305789</a>
Pages
1861–1873
Issue
3
Volume
287
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Glucose and insulin induction of bile acid synthesis: mechanisms and implication in diabetes and obesity.
Publisher
An entity responsible for making the resource available
The Journal of biological chemistry
Date
A point or period of time associated with an event in the lifecycle of the resource
2012
2012-01
Subject
The topic of the resource
*Gene Expression Regulation; Animals; Bile Acids and Salts/*biosynthesis; Cholesterol 7-alpha-Hydroxylase/genetics/*metabolism; Cytoplasmic and Nuclear/genetics/metabolism; Diabetes Mellitus; Dietary Fats/administration & dosage/adverse effects; Enzymologic; Epigenesis; Experimental/genetics/*metabolism; Fasting/metabolism; Genetic/genetics; Glucose/*metabolism/pharmacology; Insulin/*metabolism; Mice; Obesity/etiology/genetics/*metabolism; Postprandial Period/genetics; Receptors; Sweetening Agents/pharmacology; Transgenic
Creator
An entity primarily responsible for making the resource
Li Tiangang; Francl Jessica M; Boehme Shannon; Ochoa Adrian; Zhang Youcai; Klaassen Curtis D; Erickson Sandra K; Chiang John Y L
Description
An account of the resource
Bile acids facilitate postprandial absorption of nutrients. Bile acids also activate the farnesoid X receptor (FXR) and the G protein-coupled receptor TGR5 and play a major role in regulating lipid, glucose, and energy metabolism. Transgenic expression of cholesterol 7alpha-hydroxylase (CYP7A1) prevented high fat diet-induced diabetes and obesity in mice. In this study, we investigated the nutrient effects on bile acid synthesis. Refeeding of a chow diet to fasted mice increased CYP7A1 expression, bile acid pool size, and serum bile acids in wild type and humanized CYP7A1-transgenic mice. Chromatin immunoprecipitation assays showed that glucose increased histone acetylation and decreased histone methylation on the CYP7A1 gene promoter. Refeeding also induced CYP7A1 in fxr-deficient mice, indicating that FXR signaling did not play a role in postprandial regulation of bile acid synthesis. In streptozocin-induced type I diabetic mice and genetically obese type II diabetic ob/ob mice, hyperglycemia increased histone acetylation status on the CYP7A1 gene promoter, leading to elevated basal Cyp7a1 expression and an enlarged bile acid pool with altered bile acid composition. However, refeeding did not further increase CYP7A1 expression in diabetic mice. In summary, this study demonstrates that glucose and insulin are major postprandial factors that induce CYP7A1 gene expression and bile acid synthesis. Glucose induces CYP7A1 gene expression mainly by epigenetic mechanisms. In diabetic mice, CYP7A1 chromatin is hyperacetylated, and fasting to refeeding response is impaired and may exacerbate metabolic disorders in diabetes.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1074/jbc.M111.305789" target="_blank" rel="noreferrer noopener">10.1074/jbc.M111.305789</a>
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
*Gene Expression Regulation
2012
Animals
Bile Acids and Salts/*biosynthesis
Boehme Shannon
Chiang John Y L
Cholesterol 7-alpha-Hydroxylase/genetics/*metabolism
Cytoplasmic and Nuclear/genetics/metabolism
Department of Integrative Medical Sciences
Diabetes Mellitus
Dietary Fats/administration & dosage/adverse effects
Enzymologic
Epigenesis
Erickson Sandra K
Experimental/genetics/*metabolism
Fasting/metabolism
Francl Jessica M
Genetic/genetics
Glucose/*metabolism/pharmacology
Insulin/*metabolism
Klaassen Curtis D
Li Tiangang
Mice
NEOMED College of Medicine
Obesity/etiology/genetics/*metabolism
Ochoa Adrian
Postprandial Period/genetics
Receptors
Sweetening Agents/pharmacology
The Journal of biological chemistry
Transgenic
Zhang Youcai
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1152/ajpcell.00068.2016" target="_blank" rel="noreferrer noopener">http://doi.org/10.1152/ajpcell.00068.2016</a>
Pages
C212–224
Issue
2
Volume
311
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Leptin augments recruitment of IRF-1 and CREB to thrombospondin-1 gene promoter in vascular smooth muscle cells in vitro.
Publisher
An entity responsible for making the resource available
American journal of physiology. Cell physiology
Date
A point or period of time associated with an event in the lifecycle of the resource
2016
2016-08
Subject
The topic of the resource
*cAMP response element-binding protein; *interferon regulatory factor-1; *leptin; *thrombospondin-1; *transcription; *vascular smooth muscle cells; Binding Sites/genetics; Cells; Chromatin Immunoprecipitation/methods; Cultured; Cyclic AMP Response Element-Binding Protein/*metabolism; Gene Expression Regulation/genetics; Genetic/genetics; Humans; Interferon Regulatory Factor-1/*metabolism; Leptin/*metabolism; Muscle; Mutagenesis; Myocytes; Promoter Regions; Response Elements/genetics; Site-Directed/methods; Smooth; Smooth Muscle/*metabolism; Thrombospondin 1/*genetics/*metabolism; Transcription; Transcriptional Activation/genetics; Transfection/methods; Up-Regulation/genetics; Vascular/*metabolism
Creator
An entity primarily responsible for making the resource
Sahu Soumyadip; Ganguly Rituparna; Raman Priya
Description
An account of the resource
We previously reported that high pathophysiological concentrations of leptin, the adipocyte-secreted peptide, upregulate the expression of a potent proatherogenic matricellular protein, thrombospondin-1 (TSP-1), in vascular smooth muscle cells. Moreover, this regulation was found to occur at the level of transcription; however, the underlying molecular mechanisms remain unknown. The goal of the present study was to investigate the specific transcriptional mechanisms that mediate upregulation of TSP-1 expression by leptin. Primary human aortic smooth muscle cell cultures were transiently transfected with different TSP-1 gene (THBS1) promoter-linked luciferase reporter constructs, and luciferase activity in response to leptin (100 ng/ml) was assessed. We identified a long THBS1 promoter (-1270/+750) fragment with specific leptin response elements that are required for increased TSP-1 transcription by leptin. Promoter analyses, protein/DNA array and gel shift assays demonstrated activation and association of transcription factors, interferon regulatory factor-1 (IRF-1) and cAMP response element-binding protein (CREB), to the distal fragment of the THBS1 promoter in response to leptin. Supershift, chromatin immunoprecipitation, and coimmunoprecipitation assays revealed formation of a single complex between IRF-1 and CREB in response to leptin; importantly, recruitment of this complex to the THBS1 promoter mediated leptin-induced TSP-1 transcription. Finally, binding sequence decoy oligomer and site-directed mutagenesis revealed that regulatory elements for both IRF-1 (-1019 to -1016) and CREB (-1198 to -1195), specific to the distal THBS1 promoter, were required for leptin-induced TSP-1 transcription. Taken together, these findings demonstrate that leptin promotes a cooperative association between IRF-1 and CREB on the THBS1 promoter driving TSP-1 transcription in vascular smooth muscle cells.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1152/ajpcell.00068.2016" target="_blank" rel="noreferrer noopener">10.1152/ajpcell.00068.2016</a>
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
*cAMP response element-binding protein
*interferon regulatory factor-1
*leptin
*thrombospondin-1
*Transcription
*vascular smooth muscle cells
2016
American journal of physiology. Cell physiology
Binding Sites/genetics
Cells
Chromatin Immunoprecipitation/methods
Cultured
Cyclic AMP Response Element-Binding Protein/*metabolism
Department of Integrative Medical Sciences
Ganguly Rituparna
Gene Expression Regulation/genetics
Genetic/genetics
Humans
Interferon Regulatory Factor-1/*metabolism
Leptin/*metabolism
Muscle
Mutagenesis
Myocytes
NEOMED College of Medicine
Promoter Regions
Raman Priya
Response Elements/genetics
Sahu Soumyadip
Site-Directed/methods
Smooth
Smooth Muscle/*metabolism
Thrombospondin 1/*genetics/*metabolism
Transcription
Transcriptional Activation/genetics
Transfection/methods
Up-Regulation/genetics
Vascular/*metabolism
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1194/jlr.M600282-JLR200" target="_blank" rel="noreferrer noopener">http://doi.org/10.1194/jlr.M600282-JLR200</a>
Pages
373–384
Issue
2
Volume
48
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
PXR induces CYP27A1 and regulates cholesterol metabolism in the intestine.
Publisher
An entity responsible for making the resource available
Journal of lipid research
Date
A point or period of time associated with an event in the lifecycle of the resource
2007
2007-02
Subject
The topic of the resource
*Lipid Metabolism; ATP Binding Cassette Transporter; ATP Binding Cassette Transporter 1; ATP-Binding Cassette Transporters/genetics; Base Sequence; Cell Line; Cholestanetriol 26-Monooxygenase/*metabolism; Cholesterol; Cholesterol/*metabolism; Fluorinated; Genes; Genetic/drug effects; Genetic/genetics; HDL/metabolism; Hepatocytes/drug effects/enzymology/metabolism; Humans; Hydrocarbons; Hydroxycholesterols/metabolism/pharmacology; Intestinal Mucosa/metabolism; Intestines/cytology/drug effects/enzymology; Member 1; Messenger/genetics/metabolism; Molecular Sequence Data; Pregnane X Receptor; Promoter Regions; Receptors; Reporter; Response Elements/genetics; Rifampin/pharmacology; RNA; Steroid/*metabolism; Subfamily G; Sulfonamides/pharmacology; Transcription; Up-Regulation/drug effects
Creator
An entity primarily responsible for making the resource
Li Tiangang; Chen Wenling; Chiang John Y L
Description
An account of the resource
Mitochondrial sterol 27-hydroxylase (CYP27A1) catalyzes oxidative cleavage of the sterol side chain in the bile acid biosynthetic pathway in the liver and
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1194/jlr.M600282-JLR200" target="_blank" rel="noreferrer noopener">10.1194/jlr.M600282-JLR200</a>
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
*Lipid Metabolism
2007
ATP Binding Cassette Transporter
ATP Binding Cassette Transporter 1
ATP-Binding Cassette Transporters/genetics
Base Sequence
Cell Line
Chen Wenling
Chiang John Y L
Cholestanetriol 26-Monooxygenase/*metabolism
Cholesterol
Cholesterol/*metabolism
Department of Integrative Medical Sciences
Fluorinated
Genes
Genetic/drug effects
Genetic/genetics
HDL/metabolism
Hepatocytes/drug effects/enzymology/metabolism
Humans
Hydrocarbons
Hydroxycholesterols/metabolism/pharmacology
Intestinal Mucosa/metabolism
Intestines/cytology/drug effects/enzymology
Journal of lipid research
Li Tiangang
Member 1
Messenger/genetics/metabolism
Molecular Sequence Data
NEOMED College of Medicine
Pregnane X Receptor
Promoter Regions
Receptors
Reporter
Response Elements/genetics
Rifampin/pharmacology
RNA
Steroid/*metabolism
Subfamily G
Sulfonamides/pharmacology
Transcription
Up-Regulation/drug effects
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
Pages
2195–2203
Issue
12
Volume
40
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Structure and functions of human oxysterol 7alpha-hydroxylase cDNAs and gene CYP7B1.
Publisher
An entity responsible for making the resource available
Journal of lipid research
Date
A point or period of time associated with an event in the lifecycle of the resource
1999
1999-12
Subject
The topic of the resource
Humans; Animals; Mice; Cell Line; Transfection; Base Sequence; Molecular Sequence Data; Chromosome Mapping; Cytochrome P450 Family 7; DNA; Luciferases/genetics; Cytochrome P-450 Enzyme System/*genetics/metabolism; Steroid Hydroxylases/*genetics/metabolism; Hydroxycholesterols/metabolism; Codon; Northern; Blotting; Transcription; Genetic; Cloning; Molecular; Genetic/genetics; Promoter Regions; Nucleic Acid; Complementary/biosynthesis/*isolation & purification; Initiator; Regulatory Sequences
Creator
An entity primarily responsible for making the resource
Wu Z; Martin K O; Javitt N B; Chiang J Y
Description
An account of the resource
Oxysterol 7alpha-hydroxylase has broad substrate specificity for sterol metabolites and may be involved in many metabolic processes including bile acid synthesis and neurosteroid metabolism. The cloned human oxysterol 7alpha-hydroxylase (CYP7B1) cDNA encodes a polypeptide of 506 amino acid residues that shares 40% sequence identity to human cholesterol 7alpha-hydroxylase (CYP7A1), the rate-limiting enzyme in the conversion of cholesterol to bile acids in the liver. In contrast to the liver-specific expression of CYP7A1, CYP7B1 mRNA transcripts were detected in human tissues involved in steroid genesis (brain, testes, ovary, and prostate) and in bile acid synthesis (liver) and reabsorption (colon, kidney, and small intestine). The human oxysterol 7alpha-hydroxylase transiently expressed in 293/T cells was able to catalyze 7alpha-hydroxylation of
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
1999
Animals
Base Sequence
Blotting
Cell Line
Chiang J Y
Chromosome Mapping
Cloning
Codon
Complementary/biosynthesis/*isolation & purification
Cytochrome P-450 Enzyme System/*genetics/metabolism
Cytochrome P450 Family 7
Department of Integrative Medical Sciences
DNA
Genetic
Genetic/genetics
Humans
Hydroxycholesterols/metabolism
Initiator
Javitt N B
Journal of lipid research
Luciferases/genetics
Martin K O
Mice
Molecular
Molecular Sequence Data
NEOMED College of Medicine
Northern
Nucleic Acid
Promoter Regions
Regulatory Sequences
Steroid Hydroxylases/*genetics/metabolism
Transcription
Transfection
Wu Z
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1007/bf00420948" target="_blank" rel="noreferrer noopener">http://doi.org/10.1007/bf00420948</a>
Pages
615–618
Issue
6
Volume
92
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
The human cytochrome b5 gene and two of its pseudogenes are located on chromosomes 18q23, 14q31-32.1 and 20p11.2, respectively.
Publisher
An entity responsible for making the resource available
Human genetics
Date
A point or period of time associated with an event in the lifecycle of the resource
1993
1993-12
Subject
The topic of the resource
*Chromosomes; Animals; Blotting; Cell Line; Chromosome Mapping; Cricetinae; Cytochromes b5/*genetics; DNA/analysis; Genetic/genetics; Human; Humans; Hybrid Cells; In Situ Hybridization; Molecular Sequence Data; Pair 14; Pair 18; Pair 20; Polymerase Chain Reaction; Pseudogenes/*genetics; Southern; Translocation
Creator
An entity primarily responsible for making the resource
Giordano S J; Yoo M; Ward D C; Bhatt M; Overhauser J; Steggles A W
Description
An account of the resource
Using very high stringency hybridization conditions for the Southern blot hybridization analysis of hamster-human cell hybrid DNA, we were able to map the human cytochrome b5 gene and two of its pseudogenes (psgb(5)1 and psgb(5)2) unambiguously to chromosomes 18, 14, and 20. These localizations were confirmed and extended to 18q23, 14q31-32.1, and 20p11.2 by using a combination of nonisotopic in situ hybridization of chromosomal spreads and the polymerase chain reaction analysis of DNA samples isolated from somatic cell hybrids retaining deletions or translocations of chromosome 18.
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<a href="http://doi.org/10.1007/bf00420948" target="_blank" rel="noreferrer noopener">10.1007/bf00420948</a>
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Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
*Chromosomes
1993
Animals
Bhatt M
Blotting
Cell Line
Chromosome Mapping
Cricetinae
Cytochromes b5/*genetics
DNA/analysis
Genetic/genetics
Giordano S J
Human
Human genetics
Humans
Hybrid Cells
In Situ Hybridization
Molecular Sequence Data
Overhauser J
Pair 14
Pair 18
Pair 20
Polymerase Chain Reaction
Pseudogenes/*genetics
Southern
Steggles A W
Translocation
Ward D C
Yoo M