1
40
3
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
Pages
159–173
Issue
1
Volume
9
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Scanning electron microscopy and transmission electron microscopy aspects of synergistic antitumor activity of vitamin C - vitamin K3 combinations against human prostatic carcinoma cells.
Publisher
An entity responsible for making the resource available
Scanning microscopy
Date
A point or period of time associated with an event in the lifecycle of the resource
1995
1995-03
Subject
The topic of the resource
Humans; Male; Antineoplastic Agents/*pharmacology; Drug Synergism; Coloring Agents; Ascorbic Acid/*pharmacology; Carcinoma/*drug therapy/pathology; Cell Membrane/drug effects/ultrastructure; Cytoskeleton/drug effects/ultrastructure; Mitochondria/drug effects/ultrastructure; Prostatic Neoplasms/*drug therapy/pathology; Tetrazolium Salts; Thiazoles; Vitamin K/*pharmacology; Microscopy; Electron; Cultured; Scanning; Tumor Cells; Drug Therapy; Combination
Creator
An entity primarily responsible for making the resource
Gilloteaux J; Jamison J M; Venugopal M; Giammar D; Summers J L
Description
An account of the resource
A MTT/formazan assay was used to evaluate the antitumor activity of vitamin C (Vit C), vitamin K3 (Vit K3), or vitamin C:vitamin K3 combinations against a human prostatic carcinoma cell line (DU145). Both Vit C and Vit K3 alone exhibited antitumor activity, but only at elevated doses. When Vit C and Vit K3 were combined at a C:K3 ratio of 100:1 and administered to the carcinoma cells, the 50% cytotoxic concentrations (CD50) of the vitamins decreased 10- to 60-fold. Subsequently, the DU145 cells were examined with transmission and scanning electron microscopy (TEM and SEM) following a 1 hour treatment with Vit C, Vit K3, or Vit C/K3 combined at their 50% cytotoxic dose. Our morphological data suggest that vitamin treatment with individual vitamins affects the cytoskeleton, the mitochondria, and other membranous components of the cell. Treatment with the vitamin combination appears to potentiate the effects of the individual vitamin treatment. Specifically, there are abundant necrotic cells. The surviving cells display morphological defects characteristic of cell injury.
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
1995
Antineoplastic Agents/*pharmacology
Ascorbic Acid/*pharmacology
Carcinoma/*drug therapy/pathology
Cell Membrane/drug effects/ultrastructure
Coloring Agents
Combination
Cultured
Cytoskeleton/drug effects/ultrastructure
Drug Synergism
Drug Therapy
Electron
Giammar D
Gilloteaux J
Humans
Jamison J M
Male
Microscopy
Mitochondria/drug effects/ultrastructure
Prostatic Neoplasms/*drug therapy/pathology
Scanning
Scanning microscopy
Summers J L
Tetrazolium Salts
Thiazoles
Tumor Cells
Venugopal M
Vitamin K/*pharmacology
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1016/0024-3205(96)00466-3" target="_blank" rel="noreferrer noopener">http://doi.org/10.1016/0024-3205(96)00466-3</a>
Rights
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
Pages
1389-1400
Issue
17
Volume
59
Search for Full-text
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Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Synergistic antitumor activity of vitamins C and K-3 on human urologic tumor cell lines
Publisher
An entity responsible for making the resource available
Life Sciences
Date
A point or period of time associated with an event in the lifecycle of the resource
1996
1996-09
Subject
The topic of the resource
2-methyl-1; 4-naphthoquinone; bladder; cancer-chemotherapy; carcinoma cells; growth; isolated hepatocytes; l-ascorbic-acid; menadione; metabolism; mitomycin-c; oxidative stress; Pharmacology & Pharmacy; prostate; Research & Experimental Medicine; urologic neoplasms; vitamin C
Creator
An entity primarily responsible for making the resource
Venugopal M; Jamison J M; Gilloteaux J; Koch J A; Summers M; Giammar D; Sowick C; Summers J L
Description
An account of the resource
A micro-tetrazolium assay was employed to evaluate vitamin C (VC), vitamin K-3 (VK3) and vitamin C/vitamin K-3 combinations (VC/VK3) for their antitumor activity against eight human urologic tumor cell lines. While the individual vitamins exhibited antitumor activity at high concentrations, co-administration of the vitamin in a VC : VK3 ratio of 100 : 1 potentiated antitumor activity 4- to 61-fold even when exposure times were as short as 1 hour. Administration of exogenous catalase destroyed the antitumor activity of the vitamins and suggested that catalase destroyed the antitumor activity of the vitamins and suggested that hydrogen peroxide and perhaps other reactive oxygen species were involved in the antitumor mechanism of these vitamins. Electron micrographs taken in a previous study demonstrated that vitamin treatment damaged mitochondria and may have impaired ATP synthesis. Analysis of cellular ATP and thiol levels as well as DNA and protein synthesis during the first five hours following a one hour VC/VK3 treatment, revealed: a transient increase in ATP production, a substantial decrease in DNA synthesis, an increase in protein synthesis and a decrease in thiol levels. These results suggested that redox cycling of the vitamin combination increased oxidative stress until it surpassed the reducing ability of the cellular thiols and cellular or genetic damage ensured.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1016/0024-3205(96)00466-3" target="_blank" rel="noreferrer noopener">10.1016/0024-3205(96)00466-3</a>
Format
The file format, physical medium, or dimensions of the resource
Journal Article
1996
2-methyl-1
4-naphthoquinone
Bladder
cancer-chemotherapy
carcinoma cells
Giammar D
Gilloteaux J
growth
isolated hepatocytes
Jamison J M
Journal Article
Koch J A
l-ascorbic-acid
Life sciences
menadione
Metabolism
mitomycin-c
Oxidative Stress
Pharmacology & Pharmacy
Prostate
Research & Experimental Medicine
Sowick C
Summers J L
Summers M
urologic neoplasms
Venugopal M
vitamin C
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
n/a
Rights
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
Pages
159-173
Issue
1
Volume
9
Search for Full-text
Locate full-text within NEOMED Library's e-journal collections
<p>Users with a NEOMED Library login can search for full-text journal articles at the following url: <a href="https://libraryguides.neomed.edu/home">https://libraryguides.neomed.edu/home</a></p>
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Scanning Electron-microscopy And Transmission Electron-microscopy Aspects Of Synergistic Antitumor-activity Of Vitamin-c Vitamin K-3 Combinations Against Human Prostatic-carcinoma Cells
Publisher
An entity responsible for making the resource available
Scanning Microscopy
Date
A point or period of time associated with an event in the lifecycle of the resource
1995
1995-03
Subject
The topic of the resource
cancer-chemotherapy; cultured-mammalian-cells; cytoskeleton; cytotoxicity; growth-invitro; induced oxidative stress; isolated hepatocytes; l-ascorbic-acid; lines; menadione; Microscopy; prostate carcinoma; ruthenium tetroxide; toxicity; ultrastructure; vitamin-c; vitamin-k-3
Creator
An entity primarily responsible for making the resource
Gilloteaux J; Jamison J M; Venugopal M; Giammar D; Summers J L
Identifier
An unambiguous reference to the resource within a given context
n/a
Format
The file format, physical medium, or dimensions of the resource
Journal Article or Conference Abstract Publication
1995
cancer-chemotherapy
cultured-mammalian-cells
cytoskeleton
cytotoxicity
Giammar D
Gilloteaux J
growth-invitro
induced oxidative stress
isolated hepatocytes
Jamison J M
l-ascorbic-acid
lines
menadione
Microscopy
prostate carcinoma
ruthenium tetroxide
Scanning microscopy
Summers J L
toxicity
ultrastructure
Venugopal M
vitamin-c
vitamin-k-3