Adenosine-monophosphate Deaminase Histochemical Detection In Rat Skeletal-muscles
Anatomy & Morphology
Gilloteaux J; Meyer R A; Terjung R L
Anatomical Record
1980
1980
Journal Article or Conference Abstract Publication
n/a
Aging And Cardiomyopathic Changes In The Hamster Adrenal Glomerulosa
Anatomy & Morphology
Gilloteaux J; Kingsley E
Anatomical Record
1987
1987-05
Journal Article or Conference Abstract Publication
n/a
Alterations In Hamster Uterine Catalase Levels As A Result Of Estrogen-treatment - A Histoenzymatic Analysis
Science & Technology - Other Topics
Gilloteaux J; Steggles A W
Annals of the New York Academy of Sciences
1982
1982
Journal Article or Conference Abstract Publication
<a href="http://doi.org/10.1111/j.1749-6632.1982.tb21476.x" target="_blank" rel="noreferrer noopener">10.1111/j.1749-6632.1982.tb21476.x</a>
Alterations In Monoamine-oxidase Activity In The Hamster Retina Following Steroid Treatment
Cell Biology
Gilloteaux J; Steggles A W
Journal of Histochemistry & Cytochemistry
1983
1983
Journal Article or Conference Abstract Publication
n/a
ALTERATIONS IN THE SUB-CELLULAR DISTRIBUTION OF GUANYLATE-CYCLASE AND ITS RESPONSIVENESS TO NITRIC-OXIDE IN DIETHYLSTILBESTROL-INDUCED RENAL TUMORS
Oncology
Braughler J M; Gilloteaux J; Steggles A W
Cancer
1982
1982
Journal Article or Conference Abstract Publication
<a href="http://doi.org/10.1002/1097-0142(19820701)50:1%3C78::aid-cncr2820500115%3E3.0.co;2-c" target="_blank" rel="noreferrer noopener">10.1002/1097-0142(19820701)50:1%3C78::aid-cncr2820500115%3E3.0.co;2-c</a>
Altered Ionic Content In Hamster Myocardium During Progressive Cardiomyopathy - An X-ray-microanalysis Study
Cell Biology
Gilloteaux J
Biology of the Cell
1983
1983
Journal Article or Conference Abstract Publication
n/a
Amp Deaminase Histoenzymatic Staining In Rat Intrafusal Muscle-fibers And Other Skeletal-muscle Tissues
Anatomy & Morphology; Microbiology
Gilloteaux J; Meyer R A
Zeitschrift Fur Mikroskopisch-Anatomische Forschung
1983
1983
Journal Article or Conference Abstract Publication
n/a
Amp Deaminase Histoenzymology In Hamster Skeletal-muscle
Cell Biology
Gilloteaux J; Ader M
Acta Histochemica
1983
1983
Journal Article or Conference Abstract Publication
<a href="http://doi.org/10.1016/s0065-1281(83)80074-9" target="_blank" rel="noreferrer noopener">10.1016/s0065-1281(83)80074-9</a>
Antiviral activity of magnesium and magnesium/poly r(A-U) combinations against two RNA viruses.
Antiviral Agents/*pharmacology; HIV-1/*drug effects; Humans; Magnesium/*pharmacology; Microbial Sensitivity Tests; Poly A-U/*pharmacology; Vesicular stomatitis Indiana virus/*drug effects
Magnesium (Mg2+) potentiated the anti-vesicular stomatitis virus (VSV) activity of poly r(A-U) or poly r(G-C) and the anti-HIV-1 activity of poly r(A-U). Mg2+ did not affect the anti-VSV activity of poly (rI).poly (rC), poly (dA-dT).poly (dA-dT) or poly (dG-dC).poly (dG-dC). Modulation of one or more nuclear (nucleolar) processes of the host cell may be responsible for the synergistic antiviral activity.
Jamison J; Gilloteaux J; Nassiri M R; Tsai C C; Summers J
Nucleosides & nucleotides
1999
1999-07
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
<a href="http://doi.org/10.1080/07328319908044668" target="_blank" rel="noreferrer noopener">10.1080/07328319908044668</a>
Apical excrescences in the gallbladder epithelium of the female Syrian hamster in response to medroxyprogesterone.
Animals; Bile/metabolism; Cholelithiasis/*chemically induced/pathology; Cholesterol/metabolism; Cricetinae; Electron; Epithelium/ultrastructure; Female; Gallbladder/*drug effects/metabolism/ultrastructure; Medroxyprogesterone Acetate/*pharmacology/toxicity; Mesocricetus/*anatomy & histology; Microscopy; Mucus/metabolism; Ovariectomy; Scanning; Sex Factors
All the intact female Syrian hamsters treated with medroxyprogesterone (MP) for a one-month period, without dietary manipulation, display gallbladder surface epithelial changes, and intraluminal deposits. These changes include excrescences in various stages, bulging, and extrusion of material from the epithelial cells. The most striking scanning electron microscopic observations are the dramatic events, comparable to apocrine-like secretory events observed in another related study using oophorectomized hamsters. Since the hamster gallbladder does not possess mucous goblet cells, it appears that this phenomenon could be a response to the MP treatment, thus providing a larger amount of mucous product than usual with cellular material, in addition to the possible alteration in the quality of the bile following this treatment. As a result of MP treatment, intraluminal deposits were also confirmed by using light and transmission electron microscopy. In control hamsters these events were not observed, however, small blebs outlining surface epithelial cells are seen. The results in this report complement the previous studies using the male and oophorectomized Syrian hamster model subjected to similar experimental conditions.
Gilloteaux J; Karkare S; Kelly T R
The Anatomical record
1993
1993-07
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
<a href="http://doi.org/10.1002/ar.1092360308" target="_blank" rel="noreferrer noopener">10.1002/ar.1092360308</a>
ATROPHY AND HISTOENZYMOLOGY OF AMP DEAMINASE IN AGING HAMSTER SKELETAL-MUSCLE
Cell Biology
Ader M; Gilloteaux J
Biology of the Cell
1982
1905-6
Journal Article or Conference Abstract Publication
n/a
Autoschizis Of Human Ovarian Carcinoma Cells: Scanning Electron And Light Microscopy Of A New Cell Death Induced By Sodium Ascorbate: Menadione Treatment
adenocarcinoma; ascorbate; autoschizis; cancer-chemotherapy; combined vitamin-c; cultured-mammalian-cells; growth-invitro; induced oxidative stress; Instruments & Instrumentation; MDAH 2774; menadione; Microscopy; Microscopy; n-nitrosomorpholine; nuclear matrix; ovary; scanning; synergistic antitumor-activity; tumor-cells; ultrastructural aspects
Gilloteaux J; Jamison J M; Arnold D; Jarjoura D; Von Greuningen V; Summers J L
Scanning
2003
2003-05
Journal Article or Conference Abstract Publication
n/a
Autoschizis: A New Form Of Cell Death For Human Ovarian Carcinoma Cells Following Ascorbate : Menadione Treatment Nuclear And Dna Degradation
Anatomy & Morphology; ascorbate; cell; Cell Biology; combinations; combined vitamin-c; cultured-mammalian-cells; death; deoxyribonucleic-acid; DNA; growth-invitro; induced oxidative stress; k-3; menadione; Microscopy; nucleus; ovarian carcinoma; scanning electron; sodium ascorbate; synergistic antitumor-activity; ultrastructural aspects
Gilloteaux J; Jamison J M; Lorimer H E; Jarjoura D; Taper H S; Calderon P B; Neal D R; Summers J L
Tissue & Cell
2004
2004-06
Journal Article or Conference Abstract Publication
<a href="http://doi.org/10.1016/j.tice.2004.01.006" target="_blank" rel="noreferrer noopener">10.1016/j.tice.2004.01.006</a>
Calcitonin-cell Hyperplasia In Obese Zucker Rats
Anatomy & Morphology
Gilloteaux J; Jamison E; Finkelstein J A
Anatomical Record
1985
1985
Journal Article or Conference Abstract Publication
n/a
Cancer Cell Necrosis By Autoschizis: Synergism Of Antitumor Activity Of Vitamin C : Vitamin K-3 On Human Bladder Carcinoma T24 Cells
apoptosis; bladder; cancer; combinations; cultured-mammalian-cells; cytoskeleton; death; features; induced oxidative stress; Instruments & Instrumentation; mechanism; Microscopy; necrosis; potentiation; tumor; ultrastructure; vitamins
Gilloteaux J; Jamison J M; Arnold D; Ervin E; Eckroat L; Docherty J J; Neal D; Summers J L
Scanning
1998
1998-11
Journal Article or Conference Abstract Publication
n/a
Cardiomyocyte aging and hypertrophy: atrial and ventricular changes in normal and myopathic Syrian hamsters.
Female; Animals; Cricetinae; Mesocricetus; Microscopy; Aging/*pathology; Cardiomegaly/*pathology; Heart Atria/pathology/ultrastructure; Heart Ventricles/pathology/ultrastructure; Myocardium/*cytology/pathology/ultrastructure; Electron
Cardiac eccentric hypertrophy is associated with complex architectural and metabolic alterations. Atrial and ventricular myocyte diameters in cardiomyopathic (Bio 14.6) and age-matched control (F1b) Syrian hamsters were compared and histopathology was examined by electron microscopy. The mean diameter of ventricular myocyte diameters increased with age in both F1b and Bio 14.6 hamsters. Significantly greater hypertrophy was noted in the Bio 14.6 left ventricle myocytes than the F1b. Histo- and cytopathologic findings included amitotic myocardial divisions and focal myocyte necrosis in Bio hearts. Sarcomere length appeared to be similar across age and strain; however, wider sarcomeres were found in conjunction with wider diameters of myopathic left ventricle myocytes. Moreover, F1b hamsters' atrial myocytes maintained a static diameter, while those of the Bio 14.6 showed hypertrophy even preceding ventricular changes. These findings indicated that the cardiomyopathic heart is characterized by a continued fiber hypertrophy despite cardiac decompensation. Other histopathological findings included amitotic myocardial divisions, focal necrosis and sarcoplasmic widening of Z-lines. In addition, vascular smooth muscles of Bio 14.6 demonstrated vacuolization.
Gilloteaux J; Bissler J J; Kondolios P; Jarjoura D
Journal of submicroscopic cytology and pathology
1990
1990-04
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
Cell Death By Autoschizis In Tramp Prostate Carcinoma Cells As A Result Of Treatment By Ascorbate: Menadione Combination
ascorbate; autoschizis; carcinoma cells; cell death; combined vitamin-c; cultured-mammalian-cells; electron-microscopy; induced oxidative stress; kills cancer-cells; Microscopy; Microscopy; nuclease; Pathology; prostate; ribonucleoproteins; scanning electron; sodium ascorbate; synergistic antitumor-activity; TRAMP; transgenic adenocarcinoma; ultrastructural aspects
Gilloteaux J; Jamison J M; Neal D R
Ultrastructural Pathology
2005
2005-05
Journal Article or Conference Abstract Publication
<a href="http://doi.org/10.1080/01913120590951239" target="_blank" rel="noreferrer noopener">10.1080/01913120590951239</a>
CHANGES IN HAMSTER LIVER ALBUMIN SYNTHESIS DURING THE DEVELOPMENT OF CARDIOMYOPATHY
Pathology
Steggles A W; Gilloteaux J
Virchows Archiv B-Cell Pathology Including Molecular Pathology
1984
1984
Journal Article
<a href="http://doi.org/10.1007/bf02889875" target="_blank" rel="noreferrer noopener">10.1007/bf02889875</a>
Characterization of the early events in vitamin C and K3-induced death of human bladder tumor cells.
Humans; Time Factors; Drug Synergism; Microscopy; Antineoplastic Combined Chemotherapy Protocols/*pharmacology; Ascorbic Acid/administration & dosage/pharmacology; Carcinoma/*drug therapy/ultrastructure; Cell Death/*drug effects; Urinary Bladder Neoplasms/*drug therapy/ultrastructure; Vitamin K 1/administration & dosage/pharmacology; Electron; Electron/methods; Cultured/drug effects; Tumor Cells; Scanning/methods
Ervin E; Jamison J M; Gilloteaux J; Docherty J J; Jasso J; Summers J L
Scanning
1998
1998-04
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
Cytometric study of the female Syrian hamster gallbladder epithelium following sex steroid administration.
Animals; Cell Count; Cholelithiasis/*chemically induced/pathology; Cricetinae; Cytoplasm/drug effects; Drug Administration Schedule; Epithelial Cells; Epithelium/drug effects; Estrogens/*pharmacology; Female; Gallbladder/cytology/*drug effects; Mesocricetus; Progesterone/*pharmacology
This report is a cytometric study of the female Syrian hamster gallbladder epithelium following 1-, 2-, and 3-month administration of female sex steroids. Nulliparous, multiparous, young, old and pregnant hamsters were used in this study. A 1 month treatment with estrogen alone significantly increases the nuclear volume of the gallbladder epithelial cells, while E + P treatment significantly affects the nuclear volume only after a 2 month treatment. On the other hand, E + P and P treatments significantly increase the cell volumes as compared to the E-treated groups, this effect is most striking following the 1 month period. Prolonged sex steroid treatment (2 and 3 month) does not appear to influence the gallbladder epithelial cell and nuclear volumes as dramatically as that observed following the 1 month treatment. The nulliparous, progesterone-treated hamsters appear to have a greater cytoplasmic volume than the multiparous group and this is substantiated by the bulging apices and the luminal cellular excrescences observed with scanning and transmission electron microscopy. These observations are similar to those reported in ovariectomized hamsters (Gilloteaux et al., 1992). Further, the gallbladder epithelial cells and nuclei of the older female hamsters demonstrate an accentuated response to a 1 month sex steroid treatment as compared to the younger hamsters for the same treatment duration. These results enable us to hypothesize that changes induced by a short term sex steroid treatment participate in the gallstone nucleation process, while longer duration of the treatments contribute to progressive enlargement and accumulation of gallbladder calculi.
Adamiec-Beyga E; Karkare S; Kelly T R; Gilloteaux J
Tissue & cell
1993
1993-08
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
<a href="http://doi.org/10.1016/0040-8166(93)90006-7" target="_blank" rel="noreferrer noopener">10.1016/0040-8166(93)90006-7</a>
Diethylstilbestrol Induced Cystadenomatous Papilloma And Uterus Hypoplasia In The Hamster
Anatomy & Morphology
Gilloteaux J; Paul R J; Steggles A W
Anatomical Record
1982
1982
Journal Article or Conference Abstract Publication
n/a
Diethylstilbestrol Induced Uterine Abnormalities In The Syrian-hamster - Observations From Scanning Electron-microscopy
Anatomy & Morphology
Gilloteaux J; Steggles A W
Anatomical Record
1981
1981
Journal Article or Conference Abstract Publication
n/a
EFFECT OF HEAVY-RESISTANCE EXERCISE TRAINING ON MUSCLE-FIBER COMPOSITION IN YOUNG-RATS
Physiology; Sport Sciences
Yarasheski K E; Lemon P W R; Gilloteaux J
Journal of Applied Physiology
1990
1990-08
Journal Article
<a href="http://doi.org/10.1152/jappl.1990.69.2.434" target="_blank" rel="noreferrer noopener">10.1152/jappl.1990.69.2.434</a>
Effects Of Fasting On Liver Peroxisomes In Zucker Rat - A Qualitative And Quantitative Electron-microscopic Study
Cell Biology
Gilloteaux J
European Journal of Cell Biology
1986
1986
Journal Article or Conference Abstract Publication
n/a
Effects Of Hormone-treatment On The Distribution Of Monoamine-oxidase In The Syrian-hamster Uterus
Biochemistry & Molecular Biology; Cell Biology
Gilloteaux J; Steggles A W
Cellular and Molecular Biology
1983
1983
Journal Article or Conference Abstract Publication
n/a
Effects of sex steroids on the Syrian hamster liver.
Female; Animals; Cricetinae; Mesocricetus; Biliary Tract/pathology; Cholelithiasis/*chemistry/pathology; Estrogens/toxicity; Gallbladder/pathology; Gonadal Steroid Hormones/*toxicity; Liver/*drug effects/pathology/ultrastructure; Medroxyprogesterone/pharmacology; Progesterone Congeners/pharmacology; Microscopy; Electron
The primary objective of this research project was to study the role of sex steroids in the pathogenesis of cholelithiasis using the Syrian hamster as a model. In addition to the morphological examination of the gallbladder epithelium, we thought it imperative to observe the changes induced in the biliary tract in response to the sex steroid treatment. This report focuses on the morphological changes induced in the liver. The hamsters were randomly divided into 4 groups, control (C), estrogen-treated (E), estrogen and medroxyprogesterone-treated (E+MP), and medroxyprogesterone-treated (MP) groups. The E group hepatocytes demonstrated proliferation of the smooth endoplasmic reticulum, lipofuscin-like granules, aggregates of glycogen rosettes, and dense bodies. Lipid droplets in the hepatocyte cytoplasm as well as the nuclei were detected in this group. E+MP combined treatment induced an exacerbation of all the changes observed in the E group, furthermore, there appeared to be a disruption of the hepatic parenchymal architecture. The MP-treated group also exhibited the architectural changes observed in the E+MP group, but also showed sinusoidal dilation. In response to MP alone, the fatty changes in the liver appeared to be accentuated. A striking feature induced in response to MP treatment, was a focal area suggestive of adenomatous changes.
Karkare S; Adamiec-Beyga E; Gilloteaux J
Histology and histopathology
1995
1995-07
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
Endocardial surface and atrial morphological changes during development and aging.
*Aging; Animals; Cricetinae; Electron; Embryonic and Fetal Development; Endocardium/embryology/growth & development/*ultrastructure; Endothelium/ultrastructure; Female; Heart Atria/embryology/growth & development/*ultrastructure; Male; Mesocricetus; Microscopy; Microvilli/ultrastructure; Pregnancy; Scanning
Light, scanning, and transmission electron microscopic observations related to morphological changes of the right atrium as well as the atrial endocardium during development (15th embryonic day and 1 day old) and aging (560 days old) in the Syrian hamster were described and correlated. From the fetus to the adult, the atrial endocardium differentiates in parallel with, or in response to, the subjacent proliferating myocytes in the atrial wall and the trabeculae. Simultaneously, the atrium compartmentalizes grossly into a main chamber and an appendicular region. There is a progressive differentiation from a rudimentary, open chamber with primitive mural ridges in the fetal atria to a distinct, separate, atrial main chamber and appendage with a dense network of trabeculae in the adult. The fetal and neonatal endocardial, endothelial cells are convex with a central nuclear bulging and attenuated cytoplasmic extensions; the adult endocardium shows a squamous endothelium. Two cell surface specializations were observed in all age groups: microvilli and blebs or cytoplasmic protrusions. The general atrial morphology and surface endocardial changes were correlated with growth and the role of the endocardial endothelium as a barrier which controls metabolic exchanges, including the transport of atrial natriuretic factor, between the myocytes and the blood. This endothelial function appears to be essential in the fetal and neonatal age groups since no blood vessels are detected in these groups.
Gilloteaux J; Linz D
The American journal of anatomy
1989
1989-10
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
<a href="http://doi.org/10.1002/aja.1001860206" target="_blank" rel="noreferrer noopener">10.1002/aja.1001860206</a>
Endocardial Surface Changes During Hamster Atrium Development - A Tem, Sem, And Anf Immunoelectron Microscopic Study
Gilloteaux J; Linz D
1990
1990
Book/Monograph
n/a
Endocardial Surface Changes During Hamster Atrium Development - A Tem, Sem, And Anf Immunoelectron Microscopic Study
Science & Technology - Other Topics
Gilloteaux J; Linz D
Annals of the New York Academy of Sciences
1990
1990-04
Journal Article or Conference Abstract Publication
<a href="http://doi.org/10.1111/j.1749-6632.1990.tb13237.x" target="_blank" rel="noreferrer noopener">10.1111/j.1749-6632.1990.tb13237.x</a>
Endometrium Cell-surface Abnormalities In The Syrian-hamster As A Result Of Inutero Exposure To Diethylstilbestrol
Microscopy
Gilloteaux J; Steggles A W
Scanning Electron Microscopy
1985
1985
Journal Article or Conference Abstract Publication
n/a
Enhanced antiviral activity and altered subcellular distribution of magnesium/poly r(A-U) combinations.
Antiviral Agents/*pharmacology; Cells; Chromatin/drug effects; Cultured; Ethidium/*pharmacology; Humans; Magnesium/metabolism/*pharmacology; Nucleolus Organizer Region/*drug effects; Poly A-U/*pharmacology; Vesicular stomatitis Indiana virus/drug effects
When Mg2+ or ethidium bromide (EB) were combined with poly r(A-U) at a ligand/ribonucleotide ratio of 1/4, the antiviral activity of the Mg2+ and EB increased 136-fold and 154-fold. Eriochrome Blue SE was employed to visualize the subcellular distribution of Mg2+ following co-incubation of Human Foreskin Fibroblasts (HSF) with Mg2+ alone or with the Mg2+/poly r(A-U) combination. Phase contrast micrographs of these Mg(2+)-treated HSF cells as well as phase contrast and fluorescence micrographs of EB-treated or EB/poly r(A-U)-treated HSF cells illustrated that the Mg2+ (or EB)/poly r(A-U) combinations display altered subcellular distribution with the Mg2+ and EB being localized in the nucleoli and chromatin of the HSF cells. These results suggest that modulation of nuclear processes may be responsible for the enhanced antiviral activity.
Krabill K; Jamison J M; Gilloteaux J; Summers J L
Cell biology international reports
1992
1992-10
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
<a href="http://doi.org/10.1016/s0309-1651(06)80050-0" target="_blank" rel="noreferrer noopener">10.1016/s0309-1651(06)80050-0</a>
Epithelial surface changes and induction of gallstones in the male Syrian hamster gallbladder as a result of a two-month sex steroid treatment.
Male; Animals; Body Weight/drug effects; Reference Values; Cricetinae; Mesocricetus; Estradiol/*toxicity; Medroxyprogesterone/*toxicity; Cell Membrane/pathology/ultrastructure; Cholelithiasis/chemically induced/*pathology; Cytoplasmic Granules/ultrastructure; Epithelium/pathology/ultrastructure; Gallbladder/drug effects/*pathology/ultrastructure; Vacuoles/ultrastructure; Microscopy; Electron; Scanning
Transmission (TEM) and scanning (SEM) electron microscopic observations were correlated to characterize morphologic changes induced in the gallbladder of male Syrian hamsters following a two-month estradiol (E) and estradiol + medroxyprogesterone (E + MP) treatment. Compared to control (C), E-treated surface epithelial cells show pleomorphism, cytoplasmic vacuolizations, apical granules, excrescences and decapitations, and small gallstone-like deposits. Following both E + MP treatment, a large accumulation of apical granules containing acidic mucoid products, abundant intraluminal deposits and numerous fields of observation suggest that cell debris and mucous condensation could participate in the formation of the large intraluminal gallstone-like deposits detected as a result of this treatment. In control gallbladders these events were never observed. MP added to E also increases liver and gallbladder weight as well as blood lipid levels. These findings complement and confirm other previous data obtained following short steroid treatment in male, ovariectomized and intact female hamsters. In addition, these results support our hypothesis that gallstone nucleation and growth originate from multiple factors, hormonal disturbance, modulation of liver lipid metabolism, production of cell debris and mucus, can be responsible for the initial gallstone nucleation.
Gilloteaux J; Kosek E; Kelly T R
Journal of submicroscopic cytology and pathology
1993
1993-10
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
Epithelial Surface Changes And Induction Of Gallstones In The Male Syrian-hamster Gallbladder As A Result Of A 2-month Sex Steroid Treatment
biliary lipids; cholesterol gallstones; contraceptive steroids; crystal-structure; gallbladder; gallstone; hamster; human female; model bile; mucus; ovulatory cycle; Pathology; prairie dog; sex steroid; ultrastructure; x-ray-microanalysis
Gilloteaux J; Kosek E; Kelly T R
Journal of Submicroscopic Cytology and Pathology
1993
1993-10
Journal Article or Conference Abstract Publication
n/a
ESTROGEN-INDUCED ORGAN DAMAGES IN THE MALE SYRIAN-HAMSTER
Anatomy & Morphology
Steggles A W; Gilloteaux J
Anatomical Record
1981
1981
Journal Article
n/a
Evaluation of the in vitro and in vivo antitumor activities of vitamin C and K-3 combinations against human prostate cancer.
Animals; Antineoplastic Agents/*therapeutic use; Antineoplastic Combined Chemotherapy Protocols/therapeutic use; Ascorbic Acid/*therapeutic use; Humans; Male; Prostatic Neoplasms/*prevention & control; Vitamin K/*therapeutic use
Jamison J M; Gilloteaux J; Taper H S; Summers J L
The Journal of nutrition
2001
2001-01
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
<a href="http://doi.org/10.1093/jn/131.1.158S" target="_blank" rel="noreferrer noopener">10.1093/jn/131.1.158S</a>
Excessive sympathetic nervous system activity decreases myocardial contractility.
Female; Male; Animals; Rabbits; Epinephrine/blood; Norepinephrine/blood; Cisterna Magna; Hemodynamics/drug effects; Sympathetic Nervous System/drug effects/*physiology; Citrates/administration & dosage/pharmacology; Citric Acid; Extravascular Lung Water/drug effects; Heart Ventricles/drug effects/ultrastructure; Myocardial Contraction/*physiology; Ventricular Function; Veratrine/administration & dosage/pharmacology
The objective of this study was to determine whether myocardial contractility is depressed by intense activation of the sympathetic nervous system. A massive sympathetic discharge was produced by injecting veratrine or sodium citrate into the cisterna magna of anesthetized rabbits (n = 10). Two and one-half hr later, the hearts were isolated and their left ventricular (LV) performance evaluated and compared with the LV performance of hearts isolated from control animals (n = 10). LV performance was evaluated from steady-state peak isovolumic systolic and end-diastolic pressures that were generated at various end-diastolic volumes (LV function curves). The relationship between peak LV systolic pressure (or the average peak developed LV wall stress) and LV end-diastolic volume was rotated downward (P less than 0.01) in the hearts removed from rabbits treated with veratrine or citrate. The LV end-diastolic pressure or LV end-diastolic wall stress of these hearts was not different from control at any end-diastolic volume. The diminished ability of the experimental hearts to develop systolic pressure or wall stress suggests that intense sympathetic activation depressed contractility. Severely damaged myofibers, located largely in the subendocardium, were found in these hearts. Furthermore, the depressed contractility was not related to pulmonary edema since only 2 of 10 rabbits developed edema.
Pilati C F; Clark R S; Gilloteaux J; Bosso F J; Holcomb P; Maron M B
Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.)
1990
1990-03
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
<a href="http://doi.org/10.3181/00379727-193-43030" target="_blank" rel="noreferrer noopener">10.3181/00379727-193-43030</a>
Female sex steroid induced epithelial changes in the gallbladder of the ovariectomized Syrian hamster.
Animals; Cholesterol; Cricetinae; Dietary/*administration & dosage; Electron; Epithelium/drug effects/ultrastructure; Estrogens/*adverse effects; Female; Gallbladder/*drug effects/ultrastructure; Mesocricetus; Microscopy; Ovariectomy; Ovary/*physiology; Progesterone/*adverse effects; Scanning
Ovariectomized Syrian hamsters treated by female sex steroids during a 1-month period show gallbladder surface epithelial changes in the fundic area consistent with apical bulging and decapitations of the epithelial cells. These events were detected in the infundibulum and the fundic or body regions of estrogen- and estrogen+progesterone-treated hamsters. In control hamsters, these events were restricted to the region in the vicinity of the bile duct. Following steroid treatment, intraluminal deposits detected resembled Ca-bilirubinate deposits described in previous studies while decapitations are similar to endometrial epithelium changes associated with hormonal physiological changes or treatments. Moreover some small electron-dense deposits are comparable to those found in human cholesterol gallstones. This report indicates that, besides an alteration in bile composition, cell fragments originating from the surface epithelium of the bile duct and/or of the gallbladder mucosal epithelium could participate in gallstone nucleation.
Gilloteaux J; Karkare S; Ko W; Kelly T R
Tissue & cell
1992
1905-06
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
<a href="http://doi.org/10.1016/0040-8166(92)90022-y" target="_blank" rel="noreferrer noopener">10.1016/0040-8166(92)90022-y</a>
Flow cytometric and ultrastructural aspects of the synergistic antitumor activity of vitamin C-vitamin K3 combinations against human prostatic carcinoma cells.
*Prostatic Neoplasms; Antineoplastic Combined Chemotherapy Protocols/*pharmacology; Ascorbic Acid/administration & dosage; Cultured/drug effects/metabolism/ultrastructure; DNA/biosynthesis; Drug Synergism; Electron; Flow Cytometry; Hemostatics/administration & dosage; Humans; Male; Microscopy; Scanning; Thymidine/metabolism; Tritium; Tumor Cells; Vitamin K/administration & dosage
Transmission and scanning electron microscopy and flow cytometry were employed to characterize the cytotoxic effects of vitamin C (VC), vitamin K3 (VK3), or VC-VK3 combinations on a human prostate carcinoma cell line (DU145) following a 1-h vitamin treatment and a 24-h incubation in culture medium. Cells exposed to VC exhibited membranous blebs, aberrant microvillar morphology, mitochondria with swollen cristae and intramitochondrial deposits, as well as nucleoli with segregated components. VK3-treated cells displayed a damaged cytoskeleton and membranes, a cytoplasm which contained large lumen, condensed polysomes, and severely damaged mitochondria with residual bodies, and nuclei which exhibited chromatic condensation, pyknosis, and karyolysis. VC-VK3-treated cells exhibited characteristics consistent with necrosis, i.e. swollen mitochondria and swollen, achromatic nuclei with marginated chromatin and intact envelopes, while other cells displayed characteristics consistent with apoptosis, i.e. expulsion of organelle-containing blebs, margination of nuclear chromatin, and segregation of nucleolar components. Vitamin treatment also decreased DNA synthesis, induced a S/G2 block in the cell cycle, and resulted in the accumulation of fragmented DNA. These results suggested that increased oxidative stress, subsequent membrane damage, and DNA fragmentation were responsible for enhanced cytotoxicity of the vitamin combination.
Jamison J M; Gilloteaux J; Venugopal M; Koch J A; Sowick C; Shah R; Summers J L
Tissue & cell
1996
1996-12
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
<a href="http://doi.org/10.1016/s0040-8166(96)80072-3" target="_blank" rel="noreferrer noopener">10.1016/s0040-8166(96)80072-3</a>
Focal ellipsoid deposits in the atrial myocytes of Syrian hamster.
Female; Male; Animals; Immunohistochemistry; Cricetinae; Mesocricetus; Heart Atria/metabolism/*ultrastructure; Inclusion Bodies/metabolism/*ultrastructure; Microscopy; Immunoelectron
A peculiar hyaline sarcoplasmic inclusion in the atrial myocytes of old female Syrian hamsters is described. This type of inclusion resembles that described in rare human cardiomyopathy. They have also been described in other rodents and have been speculated eventually to contain atrial natriuretic factor (ANF). The present study demonstrated that they are fibrillar in nature, however, and do not contain ANF-immunoreactive product. Because their morphologic aspect is analogous to that described in human heart and in the pathology of other contractile tissues, it is suggested that they probably consist of intermediate filaments and/or associated macromolecules.
Gilloteaux J; Jennes L; Stoeckel M E; Vanderhaeghen J J
Ultrastructural pathology
1994
1994-04
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
<a href="http://doi.org/10.3109/01913129409016293" target="_blank" rel="noreferrer noopener">10.3109/01913129409016293</a>
Gallbladder And Cystic Duct Ultrastructure In Cholecystitis
Biochemistry & Molecular Biology; Cell Biology; Life Sciences & Biomedicine - Other; Topics
Gilloteaux J; Karkare S; Kelly T R
Faseb Journal
1995
1995-03
Journal Article or Conference Abstract Publication
n/a