1
40
10
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
n/a
Rights
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Pages
A1227-A1227
Issue
7
Volume
7
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Dublin Core
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Title
A name given to the resource
The Isolation And Characterization Of The Human Cytochrome-b5 Gene
Publisher
An entity responsible for making the resource available
Faseb Journal
Date
A point or period of time associated with an event in the lifecycle of the resource
1993
1993-04
Subject
The topic of the resource
Biochemistry & Molecular Biology; Cell Biology; Life Sciences & Biomedicine - Other; Topics
Creator
An entity primarily responsible for making the resource
Li X R; Yoo M; Giordano S J; Steggles A W
Identifier
An unambiguous reference to the resource within a given context
n/a
Format
The file format, physical medium, or dimensions of the resource
Journal Article or Conference Abstract Publication
1993
Biochemistry & Molecular Biology
Cell Biology
Faseb Journal
Giordano S J
Journal Article or Conference Abstract Publication
Li X R
Life Sciences & Biomedicine - Other
Steggles A W
Topics
Yoo M
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
n/a
Rights
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
Pages
568-570
Issue
5
Volume
93
Search for Full-text
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Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
A Splicing Mutation In The Cytochrome B(5) Gene From A Patient With Congenital Methemoglobinemia And Pseudohermaphrodism
Publisher
An entity responsible for making the resource available
Human Genetics
Date
A point or period of time associated with an event in the lifecycle of the resource
1994
1994-05
Subject
The topic of the resource
deficiency; enzyme; Genetics & Heredity; hereditary methemoglobinemia; human-liver; microsomes; rna
Creator
An entity primarily responsible for making the resource
Giordano S J; Kaftory A; Steggles A W
Identifier
An unambiguous reference to the resource within a given context
n/a
Format
The file format, physical medium, or dimensions of the resource
Journal Article or Conference Abstract Publication
1994
deficiency
enzyme
Genetics & Heredity
Giordano S J
hereditary methemoglobinemia
Human genetics
human-liver
Kaftory A
Microsomes
RNA
Steggles A W
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
n/a
Rights
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
Pages
1791-1791
Issue
3
Volume
53
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Dublin Core
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Title
A name given to the resource
THE HUMAN CYTOCHROME-B-5 GENE AND 4 OF ITS PSEUDOGENES ARE LOCATED ON CHROMOSOME 18Q23, 14Q31-32.1, 20P11.2, 14Q11.2-13 AND 14, RESPECTIVELY
Publisher
An entity responsible for making the resource available
American Journal of Human Genetics
Date
A point or period of time associated with an event in the lifecycle of the resource
1993
1993-09
Subject
The topic of the resource
Genetics & Heredity
Creator
An entity primarily responsible for making the resource
Steggles A W; Giordano S J; Yoo M; Overhauser J; Bhatt M; Ward D
Identifier
An unambiguous reference to the resource within a given context
n/a
Format
The file format, physical medium, or dimensions of the resource
Journal Article
1993
American Journal of Human Genetics
Bhatt M
Genetics & Heredity
Giordano S J
Journal Article
Overhauser J
Steggles A W
Ward D
Yoo M
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
Pages
13–17
Volume
5
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Identification and nucleotide sequence of the leukocyte and reticulocyte forms of rabbit cytochrome b5 mRNA.
Publisher
An entity responsible for making the resource available
SAAS bulletin, biochemistry and biotechnology
Date
A point or period of time associated with an event in the lifecycle of the resource
1992
1992-01
Subject
The topic of the resource
Animals; Amino Acid Sequence; Rabbits; Base Sequence; Polymerase Chain Reaction; Molecular Sequence Data; Nucleic Acid Hybridization; Cytochromes b5/chemistry/*genetics; Leukocytes/*chemistry; Liver/chemistry; Reticulocytes/*chemistry; RNA; Sequence Homology; Nucleic Acid; Messenger/*chemistry
Creator
An entity primarily responsible for making the resource
Giordano S J; Steggles A W
Description
An account of the resource
RNA extracted from rabbit leukocytes and reticulocytes was reverse transcribed and used in the Polymerase Chain Reaction technique along with primers designed to amplify the coding sequence of rabbit cytochrome b5. The resultant amplified products were subcloned and analyzed. Sequencing confirmed that leukocyte and liver cDNAs are homologous and encode the membrane-bound form of the protein. In contrast, reticulocytes exhibit a highly similar, but different mRNA which encodes the smaller, soluble cytochrome b5. This is the first example of a cytochrome b5 sequence from a tissue other than liver, erythrocyte or reticulocyte.
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
1992
Amino Acid Sequence
Animals
Base Sequence
Cytochromes b5/chemistry/*genetics
Giordano S J
Leukocytes/*chemistry
Liver/chemistry
Messenger/*chemistry
Molecular Sequence Data
Nucleic Acid
Nucleic Acid Hybridization
Polymerase Chain Reaction
Rabbits
Reticulocytes/*chemistry
RNA
SAAS bulletin, biochemistry and biotechnology
Sequence Homology
Steggles A W
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1016/0167-4781(93)90274-h" target="_blank" rel="noreferrer noopener">http://doi.org/10.1016/0167-4781(93)90274-h</a>
Pages
95–100
Issue
1
Volume
1172
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Differential expression of the mRNAs for the soluble and membrane-bound forms of rabbit cytochrome b5.
Publisher
An entity responsible for making the resource available
Biochimica et biophysica acta
Date
A point or period of time associated with an event in the lifecycle of the resource
1993
1993-02
Subject
The topic of the resource
Agar Gel; Amino Acid Sequence; Animals; Base Sequence; Blotting; Cell Membrane/metabolism; Cytochromes b5/*genetics; Cytosol/metabolism; DNA/genetics/isolation & purification; Electrophoresis; Exons; Leukocytes/metabolism; Liver/*metabolism; Messenger/genetics/isolation & purification/*metabolism; Molecular Sequence Data; Oligodeoxyribonucleotides; Polymerase Chain Reaction/methods; Rabbits; Reticulocytes/metabolism; RNA; Southern
Creator
An entity primarily responsible for making the resource
Giordano S J; Steggles A W
Description
An account of the resource
Total RNA was extracted from a variety of rabbit tissues and reverse transcribed for use in the polymerase chain reaction technique. Using primers designed to amplify the membrane-bound liver cytochrome b5 cDNA, products of two sizes were observed. Both hybridized strongly to a radiolabelled liver cytochrome b5 probe. Sequencing confirmed that the two types of cDNA product encoded the membrane-bound and the soluble forms of b5. Messenger RNA corresponding to the soluble cytochrome was detected in the lung, gallbladder and the adrenal gland, as well as in reticulocytes and bone marrow. This was an unexpected finding since the protein has been isolated only from erythrocytes. In contrast, membrane-bound cytochrome b5 mRNA was detected in all tissues tested, suggesting that the corresponding protein is ubiquitous in tissue distribution.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1016/0167-4781(93)90274-h" target="_blank" rel="noreferrer noopener">10.1016/0167-4781(93)90274-h</a>
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
1993
Agar Gel
Amino Acid Sequence
Animals
Base Sequence
Biochimica et biophysica acta
Blotting
Cell Membrane/metabolism
Cytochromes b5/*genetics
Cytosol/metabolism
DNA/genetics/isolation & purification
Electrophoresis
Exons
Giordano S J
Leukocytes/metabolism
Liver/*metabolism
Messenger/genetics/isolation & purification/*metabolism
Molecular Sequence Data
Oligodeoxyribonucleotides
Polymerase Chain Reaction/methods
Rabbits
Reticulocytes/metabolism
RNA
Southern
Steggles A W
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1016/0006-291x(91)91776-9" target="_blank" rel="noreferrer noopener">http://doi.org/10.1016/0006-291x(91)91776-9</a>
Pages
38–44
Issue
1
Volume
178
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
The human liver and reticulocyte cytochrome b5 mRNAs are products from a single gene.
Publisher
An entity responsible for making the resource available
Biochemical and biophysical research communications
Date
A point or period of time associated with an event in the lifecycle of the resource
1991
1991-07
Subject
The topic of the resource
*Genes; Amino Acid Sequence; Base Sequence; Cytochromes b5/blood/*genetics; Gene Library; Genetic; Humans; Liver/*metabolism; Molecular Sequence Data; Polymerase Chain Reaction; Polymorphism; Reticulocytes/*metabolism; RNA/blood/genetics/isolation & purification
Creator
An entity primarily responsible for making the resource
Giordano S J; Steggles A W
Description
An account of the resource
Using a combination of standard cDNA library screening techniques and the Polymerase Chain Reaction (PCR) we have isolated and sequenced DNA fragments corresponding to the human reticulocyte cytochrome b5 mRNA. The reticulocyte specific sequence codes for amino acids 97 and 98 only, with a TAA stop codon. The reticulocyte specific 3'non-translated sequence has 15 new nucleotides then utilizes the liver mRNA sequence from amino acid 97 onward. This indicates that the reticulocyte specific exon has 24 base pairs (bp). In addition, we have isolated sequences that are derived from a transcribed cytochrome b5 pseudogene. This transcript contains multiple mutations which prevent the synthesis of any functional protein.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1016/0006-291x(91)91776-9" target="_blank" rel="noreferrer noopener">10.1016/0006-291x(91)91776-9</a>
Rights
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Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
*Genes
1991
Amino Acid Sequence
Base Sequence
Biochemical and biophysical research communications
Cytochromes b5/blood/*genetics
Gene Library
Genetic
Giordano S J
Humans
Liver/*metabolism
Molecular Sequence Data
Polymerase Chain Reaction
Polymorphism
Reticulocytes/*metabolism
RNA/blood/genetics/isolation & purification
Steggles A W
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1007/bf00420948" target="_blank" rel="noreferrer noopener">http://doi.org/10.1007/bf00420948</a>
Pages
615–618
Issue
6
Volume
92
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
The human cytochrome b5 gene and two of its pseudogenes are located on chromosomes 18q23, 14q31-32.1 and 20p11.2, respectively.
Publisher
An entity responsible for making the resource available
Human genetics
Date
A point or period of time associated with an event in the lifecycle of the resource
1993
1993-12
Subject
The topic of the resource
*Chromosomes; Animals; Blotting; Cell Line; Chromosome Mapping; Cricetinae; Cytochromes b5/*genetics; DNA/analysis; Genetic/genetics; Human; Humans; Hybrid Cells; In Situ Hybridization; Molecular Sequence Data; Pair 14; Pair 18; Pair 20; Polymerase Chain Reaction; Pseudogenes/*genetics; Southern; Translocation
Creator
An entity primarily responsible for making the resource
Giordano S J; Yoo M; Ward D C; Bhatt M; Overhauser J; Steggles A W
Description
An account of the resource
Using very high stringency hybridization conditions for the Southern blot hybridization analysis of hamster-human cell hybrid DNA, we were able to map the human cytochrome b5 gene and two of its pseudogenes (psgb(5)1 and psgb(5)2) unambiguously to chromosomes 18, 14, and 20. These localizations were confirmed and extended to 18q23, 14q31-32.1, and 20p11.2 by using a combination of nonisotopic in situ hybridization of chromosomal spreads and the polymerase chain reaction analysis of DNA samples isolated from somatic cell hybrids retaining deletions or translocations of chromosome 18.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1007/bf00420948" target="_blank" rel="noreferrer noopener">10.1007/bf00420948</a>
Rights
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Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
*Chromosomes
1993
Animals
Bhatt M
Blotting
Cell Line
Chromosome Mapping
Cricetinae
Cytochromes b5/*genetics
DNA/analysis
Genetic/genetics
Giordano S J
Human
Human genetics
Humans
Hybrid Cells
In Situ Hybridization
Molecular Sequence Data
Overhauser J
Pair 14
Pair 18
Pair 20
Polymerase Chain Reaction
Pseudogenes/*genetics
Southern
Steggles A W
Translocation
Ward D C
Yoo M
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1007/bf00202825" target="_blank" rel="noreferrer noopener">http://doi.org/10.1007/bf00202825</a>
Pages
568–570
Issue
5
Volume
93
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
A splicing mutation in the cytochrome b5 gene from a patient with congenital methemoglobinemia and pseudohermaphrodism.
Publisher
An entity responsible for making the resource available
Human genetics
Date
A point or period of time associated with an event in the lifecycle of the resource
1994
1994-05
Subject
The topic of the resource
*DNA; *Mutation; Base Sequence; Cytochromes b5/*genetics; Disorders of Sex Development/*enzymology/genetics; DNA Primers/chemistry; Gene Deletion; Humans; Leukocytes/metabolism; Male; Messenger/metabolism; Methemoglobinemia/*congenital/*enzymology; Molecular Sequence Data; Polymerase Chain Reaction; Recombinant; Reticulocytes/metabolism; RNA
Creator
An entity primarily responsible for making the resource
Giordano S J; Kaftory A; Steggles A W
Description
An account of the resource
We have analyzed reticulocyte and leukocyte mRNAs isolated from a patient with congenital methemoglobinemia and pseudohermaphrodism. The cytochrome b5 cDNA sequences were amplified using specific oligonucleotide primers and the polymerase chain reaction (PCR). DNA sequencing indicated that there was a 16-bp deletion in the cDNA leading to a new, in-frame stop signal and resulting in a truncated protein of 45 amino acids. Genomic DNA was analyzed, and the molecular lesion was shown to be an AG–\textgreaterGG alteration in the 3' splicing junction of intron 1. The splice site alteration leads to the usage of the nearest AG as an alternative splice site, resulting in a 16-bp deletion in the mRNA. All of the studies on reticulocyte mRNA and genomic DNA indicated that the patient was homozygous for the lesion.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1007/bf00202825" target="_blank" rel="noreferrer noopener">10.1007/bf00202825</a>
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
*DNA
*Mutation
1994
Base Sequence
Cytochromes b5/*genetics
Disorders of Sex Development/*enzymology/genetics
DNA Primers/chemistry
Gene Deletion
Giordano S J
Human genetics
Humans
Kaftory A
Leukocytes/metabolism
Male
Messenger/metabolism
Methemoglobinemia/*congenital/*enzymology
Molecular Sequence Data
Polymerase Chain Reaction
Recombinant
Reticulocytes/metabolism
RNA
Steggles A W
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1006/geno.1993.1331" target="_blank" rel="noreferrer noopener">http://doi.org/10.1006/geno.1993.1331</a>
Pages
348–354
Issue
2
Volume
17
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
The isolation and characterization of the bovine cytochrome b5 gene, and a transcribed pseudogene.
Publisher
An entity responsible for making the resource available
Genomics
Date
A point or period of time associated with an event in the lifecycle of the resource
1993
1993-08
Subject
The topic of the resource
*Pseudogenes; Amino Acid Sequence; Animals; Base Sequence; Blotting; Cattle/*genetics; Cytochromes b5/*genetics; DNA/genetics/isolation & purification; Exons; Gene Expression; Genetic; Genomic Library; Humans; Introns; Messenger/biosynthesis/metabolism; Molecular Sequence Data; Nucleic Acid; Nucleic Acid Conformation; Oligodeoxyribonucleotides; Rabbits; Restriction Mapping; Reticulocytes/metabolism; RNA; RNA Precursors/chemistry/metabolism; Sequence Homology; Southern; Transcription
Creator
An entity primarily responsible for making the resource
Cristiano R J; Giordano S J; Steggles A W
Description
An account of the resource
This is the first isolation and characterization of a cytochrome b5(b5) gene. The bovine b5 gene is quite large, spanning about 28 kb and contains six exons. One of these exons appears to code for a reticulocyte-specific sequence similar to that described for human and rabbit b5. All of the splicing junctions conform to the GT-AG consensus rule. The 5' flanking sequence has no obvious TATA box, two CAAT boxes, and contains several G:C-rich SpI motifs indicative of a house-keeping gene. In reticulocyte mRNA we found evidence for a transcribed b5 pseudogene, but could not detect sequences coding for the soluble form of b5. We conclude that the soluble form of b5 is derived from the membrane-bound b5 by a post-translational mechanism.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1006/geno.1993.1331" target="_blank" rel="noreferrer noopener">10.1006/geno.1993.1331</a>
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
*Pseudogenes
1993
Amino Acid Sequence
Animals
Base Sequence
Blotting
Cattle/*genetics
Cristiano R J
Cytochromes b5/*genetics
DNA/genetics/isolation & purification
Exons
Gene Expression
Genetic
Genomic Library
Genomics
Giordano S J
Humans
Introns
Messenger/biosynthesis/metabolism
Molecular Sequence Data
Nucleic Acid
Nucleic Acid Conformation
Oligodeoxyribonucleotides
Rabbits
Restriction Mapping
Reticulocytes/metabolism
RNA
RNA Precursors/chemistry/metabolism
Sequence Homology
Southern
Steggles A W
Transcription
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1006/bbrc.1995.1582" target="_blank" rel="noreferrer noopener">http://doi.org/10.1006/bbrc.1995.1582</a>
Pages
894–900
Issue
3
Volume
209
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
The isolation and characterization of the human cytochrome b5 gene.
Publisher
An entity responsible for making the resource available
Biochemical and biophysical research communications
Date
A point or period of time associated with an event in the lifecycle of the resource
1995
1995-04
Subject
The topic of the resource
Animals; Base Sequence; Cattle; Cell Line; Cloning; Cosmids; Cultured; Cytochromes b5/biosynthesis/*genetics; DNA Primers; DNA/genetics/isolation & purification; Gene Library; Genetic; Hominidae/*genetics; Humans; Introns; Luciferases/biosynthesis; Messenger/analysis/biosynthesis; Molecular; Molecular Sequence Data; Polymerase Chain Reaction; Promoter Regions; Rabbits; RNA; Transfection; Tumor Cells
Creator
An entity primarily responsible for making the resource
Li X R; Giordano S J; Yoo M; Steggles A W
Description
An account of the resource
From a series of lambda and cosmid libraries, we isolated DNA sequences corresponding to the complete coding region for the human cytochrome b5 (CYB5) gene. The overall gene organization was the same as the bovine and rabbit CYB5 genes. One cosmid clone containing exon I plus the 5' flanking region was extensively characterized. From this clone we obtained 2000 bp of 5' flanking sequence, containing several distinctive GC rich regions, potential trans-acting factor binding sites and a 74 bp direct repeat. A series of deletion constructs were made in the pGL2 luciferase vector and then used to transfect HepG2 and K562 cells. The data obtained suggest the presence of two promotors and one silencer region in the analyzed 5' sequence.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1006/bbrc.1995.1582" target="_blank" rel="noreferrer noopener">10.1006/bbrc.1995.1582</a>
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
1995
Animals
Base Sequence
Biochemical and biophysical research communications
Cattle
Cell Line
Cloning
Cosmids
Cultured
Cytochromes b5/biosynthesis/*genetics
DNA Primers
DNA/genetics/isolation & purification
Gene Library
Genetic
Giordano S J
Hominidae/*genetics
Humans
Introns
Li X R
Luciferases/biosynthesis
Messenger/analysis/biosynthesis
Molecular
Molecular Sequence Data
Polymerase Chain Reaction
Promoter Regions
Rabbits
RNA
Steggles A W
Transfection
Tumor Cells
Yoo M