1 40 1 Text A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text. URL Address <a href="http://doi.org/10.3109/10837450.2014.892130" target="_blank" rel="noreferrer noopener">http://doi.org/10.3109/10837450.2014.892130</a> Pages 497–506 Issue 4 Volume 20 Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Brain-targeted delivery of doxorubicin using glutathione-coated nanoparticles for brain cancers. Publisher An entity responsible for making the resource available Pharmaceutical development and technology Date A point or period of time associated with an event in the lifecycle of the resource 2015 2015-06 Subject The topic of the resource Antibiotics; Animals; *Drug Delivery Systems; Rats; Cell Line; Polylactic Acid-Polyglycolic Acid Copolymer; Blood-Brain Barrier/*metabolism; Brain cancer; Brain Neoplasms/drug therapy/metabolism; brain-targeted delivery; doxorubicin; Doxorubicin/*administration & dosage/pharmacokinetics; Drug Carriers/*chemistry/metabolism; Glutathione/*chemistry/metabolism; Lactic Acid/chemistry/metabolism; Nanoparticles/*chemistry/metabolism; PLGA-PEG NP; Polyethylene Glycols/chemistry/metabolism; Polyglycolic Acid/chemistry/metabolism; Tumor; Antineoplastic/*administration & dosage/pharmacokinetics Creator An entity primarily responsible for making the resource Geldenhuys Werner; Wehrung Daniel; Groshev Anastasia; Hirani Anjali; Sutariya Vijaykumar Description An account of the resource OBJECTIVES: To prepare and characterize in vitro a novel brain-targeted delivery of doxorubicin using glutathione-coated nanoparticles (NPs) for the treatment of brain cancer. METHODS: Doxorubicin-loaded NPs were prepared by the nanoprecipitation method using PLGA-COOH (dl-lactide-co-glycolide). The NPs were coated with a glutathione-PEG conjugate (PEG-GSH) in order to target delivery to the brain. The NPs were characterized via in vitro studies to determine particle size, drug release, cellular uptake, immunofluorescence study, cytotoxic assay, and in vitro blood-brain barrier (BBB) assay. RESULTS: The NPs showed a particle size suitable for BBB permeation (particle size around 200 nm). The in vitro release profile of the NPs exhibited no initial burst release and showed sustained drug release for up to 96 h. The immunofluorescence study showed the glutathione coating does not interfere with the drug release. Furthermore, in vitro BBB Transwell study showed significantly higher permeation of the doxorubicin-loaded NPs compared with the free doxorubicin solution through the coculture of rat brain endothelial (RBE4) and C6 astrocytoma cells (p \textless 0.05). CONCLUSIONS: We conclude that the initial in vitro characterization of the NPs demonstrates potential in delivering doxorubicin to cancer cells with possible future application in targeting brain cancers in vivo. Identifier An unambiguous reference to the resource within a given context <a href="http://doi.org/10.3109/10837450.2014.892130" target="_blank" rel="noreferrer noopener">10.3109/10837450.2014.892130</a> Rights Information about rights held in and over the resource Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s). *Drug Delivery Systems 2015 Animals Antibiotics Antineoplastic/*administration & dosage/pharmacokinetics Blood-Brain Barrier/*metabolism Brain cancer Brain Neoplasms/drug therapy/metabolism brain-targeted delivery Cell Line Doxorubicin Doxorubicin/*administration & dosage/pharmacokinetics Drug Carriers/*chemistry/metabolism Geldenhuys Werner Glutathione/*chemistry/metabolism Groshev Anastasia Hirani Anjali Lactic Acid/chemistry/metabolism Nanoparticles/*chemistry/metabolism Pharmaceutical development and technology PLGA-PEG NP Polyethylene Glycols/chemistry/metabolism Polyglycolic Acid/chemistry/metabolism Polylactic Acid-Polyglycolic Acid Copolymer Rats Sutariya Vijaykumar Tumor Wehrung Daniel