Modulation of Mast Cell Proliferative and Inflammatory Responses by Leukotriene D-4 and Stem Cell Factor Signaling Interactions
activation; Cell Biology; cysteinyl leukotrienes; fc-epsilon-ri; immune; induced airway hyperreactivity; innate; kit ligand; Physiology; protein-kinase-c; receptor; survival
Mast cells (MCs) are important effector cells in asthma and pulmonary inflammation, and their proliferation and maturation is maintained by stem cell factor (SCF) via its receptor, c-Kit. Cysteinyl leukotrienes (cys-LTs) are potent inflammatory mediators that signal through CysLT(1)R and CysLT(2)R located on the MC surface, and they enhance MC inflammatory responses. However, it is not known if SCF and cys-LTs cross-talk and influence MC hyperplasia and activation in inflammation. Here, we report the concerted effort of the growth factor SCF and the inflammatory mediator LTD4 in MC activation. Stimulation of MCs by LTD4 in the presence of SCF enhances c-Kit-mediated proliferative responses. Similarly, SCF synergistically enhances LTD4-induced calcium, c-fos expression and phosphorylation, as well as MIP1 generation in MCs. These findings suggest that integration of SCF and LTD4 signals may contribute to MC hyperplasia and hyper-reactivity during airway hyper-response and inflammation. J. Cell. Physiol. 230: 595-602, 2015. (c) 2014 Wiley Periodicals, Inc., A Wiley Company
Al-Azzam N; Kondeti V; Duah E; Gombedza F; Thodeti C K; Paruchuri S
Journal of Cellular Physiology
2015
2015-03
Journal Article or Conference Abstract Publication
<a href="http://doi.org/10.1002/jcp.24777" target="_blank" rel="noreferrer noopener">10.1002/jcp.24777</a>
NADPH Oxidase 4 Expression Is Increased through TRPV4 Channel in D. farinae-Induced Airway Remodeling and TGF-beta 1-Mediated Fibroblast Differentiation into Myofibroblasts
Biochemistry & Molecular Biology; Cell Biology; Life Sciences & Biomedicine - Other; Topics
Al-Azzam N Z; Gombedza F; Snyderman M; Thodeti C; Paruchuri S
Faseb Journal
2017
2017-04
Journal Article or Conference Abstract Publication
n/a
Cysteinyl Leukotrienes Regulate Endothelial Cell Inflammatory And Proliferative Signals Through Cyslt(2) And Cyslt(1) Receptors
atherosclerosis; barrier; dysfunction; expression; gamma; intestinal epithelial-cells; ischemia-reperfusion injury; migration; pathway; Science & Technology - Other Topics; thrombin
Duah E; Adapala R K; Al-Azzam N; Kondeti V; Gombedza F; Thodeti C K; Paruchuri S
Scientific Reports
2013
2013-11
Journal Article or Conference Abstract Publication
<a href="http://doi.org/10.1038/srep03274" target="_blank" rel="noreferrer noopener">10.1038/srep03274</a>