1 40 1 Text A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text. URL Address <a href="http://doi.org/10.1016/j.biomaterials.2007.12.015" target="_blank" rel="noreferrer noopener">http://doi.org/10.1016/j.biomaterials.2007.12.015</a> Rights Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s). Pages 1676-1685 Issue 11 Volume 29 Search for Full-text Locate full-text within NEOMED Library's e-journal collections <p>Users with a NEOMED Library login can search for full-text journal articles at the following url: <a href="https://libraryguides.neomed.edu/home">https://libraryguides.neomed.edu/home</a></p> Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Affinity Manipulation Of Surface-conjugated Rgd Peptide To Modulate Binding Of Liposomes To Activated Platelets Publisher An entity responsible for making the resource available Biomaterials Date A point or period of time associated with an event in the lifecycle of the resource 2008 2008-04 Subject The topic of the resource cyclic RGD; delivery systems; drug-eluting stents; Engineering; insight; integrin alpha(iib)beta(3); liposomes; Materials Science; nanoparticles; peptide modification; platelet-targeting; responses; restenosis; therapy Creator An entity primarily responsible for making the resource Huang G F; Zhou Z M; Srinivasan R; Penn M S; Kottke-Marchant K; Marchant R E; Gupta A S Description An account of the resource Platelet adhesion, activation and fibrinogen-mediated aggregation are primary events in vascular thrombosis and occlusion. An injectable delivery system that can carry thrombolytics selectively to the sites of active platelet aggregation has immense potential in minimally invasive targeted therapy of vascular occlusion. To this end we are studying liposomes surface-modified by fibrinogen-mimetic RGD motifs that can selectively target and bind integrin GPIIb-IIIa on activated platelets. Here we report liposome surface-modification with a conformationally constrained high affinity cyclic RGD motif to modulate the GPIIb-IIIa-binding capability of the liposomes. Such affinity enhancement is important for practical in vivo applications to compete with native fibrinogen towards binding GPIIb-IIIa. The platelet-binding of RGD-modified liposomes was studied by fluorescence and scanning electron microscopy, and flow cytometry, in vitro. Binding of RGD-modified liposomes was also tested in vivo in a rat carotid injury model and analyzed ex vivo by fluorescence microscopy. The results from all experiments show that cyclic RGD-liposomes bind activated platelets significantly higher compared to linear RGD-liposomes. Hence, the results establish the feasibility of modulating the platelet-targeting and binding ability of vascularly targeted liposomes by manipulating the affinity of surface-modifying ligands. (c) 2008 Elsevier Ltd. All rights reserved. Identifier An unambiguous reference to the resource within a given context <a href="http://doi.org/10.1016/j.biomaterials.2007.12.015" target="_blank" rel="noreferrer noopener">10.1016/j.biomaterials.2007.12.015</a> Format The file format, physical medium, or dimensions of the resource Journal Article or Conference Abstract Publication 2008 Biomaterials cyclic RGD delivery systems Drug-Eluting Stents Engineering Gupta A S Huang G F insight integrin alpha(iib)beta(3) Journal Article or Conference Abstract Publication Kottke-Marchant K liposomes Marchant R E Materials Science Nanoparticles Penn M S peptide modification platelet-targeting responses Restenosis Srinivasan R therapy Zhou Z M