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Text
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URL Address
<a href="http://doi.org/10.1002/bip.21457" target="_blank" rel="noreferrer noopener">http://doi.org/10.1002/bip.21457</a>
Pages
207–221
Issue
2
Volume
96
Dublin Core
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Title
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Design and use of peptide-based antibodies decreasing superoxide production by mitochondrial complex I and complex II.
Publisher
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Biopolymers
Date
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2011
1905-7
Subject
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*Electron Transport Complex I/antagonists & inhibitors/chemistry/immunology/metabolism; *Electron Transport Complex II/antagonists & inhibitors/chemistry/immunology/metabolism; *Mitochondria; *Mitochondrial Proteins/antagonists & inhibitors/chemistry/immunology/metabolism; *Peptides/chemistry/immunology/metabolism; Amino Acid Motifs; Animals; Cattle; Female; Heart/chemistry/immunology/metabolism; Protein Structure; Rabbits; Tertiary
Creator
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Kang Patrick T; Yun June; Kaumaya Pravin P T; Chen Yeong-Renn
Description
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Mitochondria are the major source of reactive oxygen species. Both complex I and complex II mediate O2*- production in mitochondria and host reactive protein thiols. To explore the functions of the specific domains involved in the redox modifications of complexes I and II, various peptide-based antibodies were generated against these complexes, and their inhibitory effects were subsequently measured. The redox domains involved in S-glutathionylation and nitration, as well as the binding 2011. motif of the iron-sulfur cluster (N1a) of the complexes I and II were utilized to design B-cell epitopes for generating antibodies. The effect of antibody binding on enzyme-mediated O2*- generation was measured by EPR spin trapping. Binding of either antibody AbGSCA206 or AbGSCB367 against glutathione (GS)-binding domain to complex I inhibit its O2*- generation, but does not affect electron transfer efficiency. Binding of antibody (Ab24N1a) against the binding motif of N1a to complex I modestly suppresses both O2*- generation and electron transfer efficiency. Binding of either antibody Ab75 or Ab24 against nonredox domain decreases electron leakage production. In complex II, binding of antibody AbGSC90 against GS-binding domain to complex II marginally decreases both O2*- generation and electron transfer activity. Binding of antibody AbY142 to complex II against the nitrated domain modestly inhibits electron leakage, but does not affect the electron transfer activity of complex II. In conclusion, mediation of O2*- generation by complexes I and II can be regulated by specific redox and nonredox domains.
Identifier
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<a href="http://doi.org/10.1002/bip.21457" target="_blank" rel="noreferrer noopener">10.1002/bip.21457</a>
Rights
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Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
*Electron Transport Complex I/antagonists & inhibitors/chemistry/immunology/metabolism
*Electron Transport Complex II/antagonists & inhibitors/chemistry/immunology/metabolism
*Mitochondria
*Mitochondrial Proteins/antagonists & inhibitors/chemistry/immunology/metabolism
*Peptides/chemistry/immunology/metabolism
2011
Amino Acid Motifs
Animals
Biopolymers
Cattle
Chen Yeong-Renn
Department of Integrative Medical Sciences
Female
Heart/chemistry/immunology/metabolism
Kang Patrick T
Kaumaya Pravin P T
NEOMED College of Medicine
Protein Structure
Rabbits
Tertiary
Yun June