1
40
2
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1080/03008200902721887" target="_blank" rel="noreferrer noopener">http://doi.org/10.1080/03008200902721887</a>
Pages
307–314
Issue
5
Volume
50
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Histochemical analyses of tissue-engineered human menisci.
Publisher
An entity responsible for making the resource available
Connective tissue research
Date
A point or period of time associated with an event in the lifecycle of the resource
2009
1905-7
Subject
The topic of the resource
*Tissue Scaffolds; Animals; Cell Differentiation/physiology; Cell Proliferation; Cell Shape/physiology; Cells; Chondrocytes/*cytology/*metabolism/transplantation; Chondrogenesis/physiology; Collagen/metabolism; Cultured; Fibrocartilage/cytology/metabolism/transplantation; Graft Survival/physiology; Heterologous/methods; Histocytochemistry; Humans; Male; Menisci; Mice; Middle Aged; Nude; Phenotype; Polyglycolic Acid/pharmacology/therapeutic use; Tibial/*cytology/*metabolism/transplantation; Tissue Engineering/*methods; Transplantation
Creator
An entity primarily responsible for making the resource
Schoenfeld Andrew J; Jacquet Robin; Lowder Elizabeth; Doherty Alison; Leeson Mark C; Landis William J
Description
An account of the resource
The field of tissue engineering remains one of the least explored areas of current meniscal research but holds great promise. In this investigation, meniscal fibrochondrocytes were isolated from fresh human meniscal tissue and seeded onto synthetic polyglycolic acid (PGA) scaffolds. Constructs were implanted into the dorsal subcutaneous space of athymic nude mice. Control scaffolds, devoid of meniscal cells, were simultaneously implanted in additional mice. Constructs were harvested over 12 weeks and treated with a variety of histochemical stains to analyze general specimen morphology, cellular viability and proliferation, and collagen secretion. Results indicate that meniscal fibrochondrocyte proliferation increased over the time of implantation with cellular consolidation occurring as the PGA scaffolding was progressively hydrolyzed. Collagen production also increased over time. There were favorable similarities between constructs and human meniscal controls in terms of cellular morphology, phenotypic expression, and collagen production. These initial findings demonstrate procedures supporting proliferation of meniscal fibrochondrocytes, expression of fibrochondral phenotype, and the formation of putative meniscal tissue.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1080/03008200902721887" target="_blank" rel="noreferrer noopener">10.1080/03008200902721887</a>
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
*Tissue Scaffolds
2009
Animals
Cell Differentiation/physiology
Cell Proliferation
Cell Shape/physiology
Cells
Chondrocytes/*cytology/*metabolism/transplantation
Chondrogenesis/physiology
Collagen/metabolism
Connective tissue research
Cultured
Doherty Alison
Fibrocartilage/cytology/metabolism/transplantation
Graft Survival/physiology
Heterologous/methods
Histocytochemistry
Humans
Jacquet Robin
Landis William J
Leeson Mark C
Lowder Elizabeth
Male
Menisci
Mice
Middle Aged
Nude
Phenotype
Polyglycolic Acid/pharmacology/therapeutic use
Schoenfeld Andrew J
Tibial/*cytology/*metabolism/transplantation
Tissue Engineering/*methods
Transplantation
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1007/bf00495009" target="_blank" rel="noreferrer noopener">http://doi.org/10.1007/bf00495009</a>
Pages
1–13
Issue
1
Volume
92
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Innervation of regenerated spindles in muscle grafts of the rat.
Publisher
An entity responsible for making the resource available
Histochemistry
Date
A point or period of time associated with an event in the lifecycle of the resource
1989
1905-06
Subject
The topic of the resource
Female; Animals; Rats; Cell Differentiation; *Nerve Regeneration; Adenosine Triphosphatases/analysis; Graft Survival; Histocytochemistry; Motor Neurons/enzymology/metabolism/*physiology; Muscle Spindles/enzymology/*physiology; Muscles/transplantation; Nerve Fibers/enzymology/physiology; Neurons; Inbred Strains; Afferent/enzymology/metabolism/*physiology
Creator
An entity primarily responsible for making the resource
Walro J M; Kucera J; Cui F; Staffeld C G
Description
An account of the resource
Features of the nerve supply and the encapsulated fibers of muscle spindles were assessed in grafted and normal extensor digitorum longus (EDL) muscles of rats by analysis of serial 10-microns frozen transverse sections stained for enzymes which delineated motor and sensory endings, oxidative capacity and muscle fiber type. The number of fibers was significantly more variable, and branched fibers were more frequently observed in regenerated spindles than in control spindles. Forty-eight percent of regenerated spindles received sensory innervation. Spindles reinnervated by afferents had a larger periaxial space than did spindles which were not reinnervated by afferents. Regenerated fibers innervated by afferents had small cross-sectional areas, equatorial regions with myofibrils restricted to the periphery of fibers, unpredictable patterns of nonuniform and nonreversible staining along the length of the fiber for 'myofibrillar' adenosine triphosphatase (mATPase) after acid and alkaline preincubation. In contrast, regenerated fibers devoid of sensory innervation resembled extrafusal fibers in that they usually exhibited myofibrils throughout the length of the fiber, no central aggregations of myonuclei, uniform staining for mATPase and a reversal of staining for mATPase after preincubation in an acid or alkaline medium. Approximately thirty percent of encapsulated fibers devoid of sensory innervation stained analogous to a type I extrafusal fiber, a pattern of staining never observed in intrafusal fibers of normal spindles. Groups of encapsulated fibers all exhibiting this pattern of staining reflect that either these fibers may have been innervated by collaterals of skeletomotor axons that originally innervated type I extrafusal fibers or that fibers innervated by only fusimotor neurons express patterns of staining for mATPase similar to extrafusal fibers in the absence of sensory innervation. Sensory innervation may also influence the reestablishment of multiple sites of motor endings on regenerated intrafusal fibers. Those regenerated fibers innervated by afferents had more motor endings than did regenerated fibers devoid of sensory innervation. Differences in size, morphology, and patterns of staining for mATPase and numbers of motor endings between fibers innervated by afferents and fibers devoid of sensory innervation reflect that afferents can influence the differentiation of muscle cells and the reestablishment of motor innervation other than during the late prenatal/early postnatal period when muscle spindles form and differentiate in rats.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1007/bf00495009" target="_blank" rel="noreferrer noopener">10.1007/bf00495009</a>
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
*Nerve Regeneration
1989
Adenosine Triphosphatases/analysis
Afferent/enzymology/metabolism/*physiology
Animals
Cell Differentiation
Cui F
Female
Graft Survival
Histochemistry
Histocytochemistry
Inbred Strains
Kucera J
Motor Neurons/enzymology/metabolism/*physiology
Muscle Spindles/enzymology/*physiology
Muscles/transplantation
Nerve Fibers/enzymology/physiology
Neurons
Rats
Staffeld C G
Walro J M