1 40 1 Text A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text. URL Address <a href="http://doi.org/10.1007/s11095-021-03046-4" target="_blank" rel="noreferrer noopener">http://doi.org/10.1007/s11095-021-03046-4</a> ISSN 1573-904X Search for Full-text Locate full-text within NEOMED Library's e-journal collections <a href="http://neomed.idm.oclc.org/login?url=http://doi.org/10.1007/s11095-021-03046-4" target="_blank" rel="noreferrer noopener">NEOMED Full-text Holding (if available) - Proxy DOI: 10.1007/s11095-021-03046-4</a> <p>Users with a NEOMED Library login can search for full-text journal articles at the following url: <a href="https://libraryguides.neomed.edu/home">https://libraryguides.neomed.edu/home</a></p> Update Year & Number May 2021 List NEOMED College NEOMED College of Medicine NEOMED Department Department of Integrative Medical Sciences Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource The Mitochondrial mitoNEET Ligand NL-1 Is Protective in a Murine Model of Transient Cerebral Ischemic Stroke. Publisher An entity responsible for making the resource available Pharmaceutical Research Date A point or period of time associated with an event in the lifecycle of the resource 2021 2021-05-12 Subject The topic of the resource Bioenergetics; CDGSH; iron-sulfur; OXPHOS; PAMPA; t-MCAO Creator An entity primarily responsible for making the resource Saralkar P; Mdzinarishvili A; Arsiwala TA; Lee Y; Sullivan PG; Pinti MV; Hollander JM; Kelley EE; Ren X; Hu H; Simpkins J; Brown C; Hazlehurst LE; Huber JD; Geldenhuys WJ Description An account of the resource Purpose: Therapeutic strategies to treat ischemic stroke are limited due to the heterogeneity of cerebral ischemic injury and the mechanisms that contribute to the cell death. Since oxidative stress is one of the primary mechanisms that cause brain injury post-stroke, we hypothesized that therapeutic targets that modulate mitochondrial function could protect against reperfusion-injury after cerebral ischemia, with the focus here on a mitochondrial protein, mitoNEET, that modulates cellular bioenergetics.; Method: In this study, we evaluated the pharmacology of the mitoNEET ligand NL-1 in an in vivo therapeutic role for NL-1 in a C57Bl/6 murine model of ischemic stroke.; Results: NL-1 decreased hydrogen peroxide production with an IC 50 of 5.95 μM in neuronal cells (N2A). The in vivo activity of NL-1 was evaluated in a murine 1 h transient middle cerebral artery occlusion (t-MCAO) model of ischemic stroke. We found that mice treated with NL-1 (10 mg/kg, i.p.) at time of reperfusion and allowed to recover for 24 h showed a 43% reduction in infarct volume and 68% reduction in edema compared to sham-injured mice. Additionally, we found that when NL-1 was administered 15 min post-t-MCAO, the ischemia volume was reduced by 41%, and stroke-associated edema by 63%.; Conclusion: As support of our hypothesis, as expected, NL-1 failed to reduce stroke infarct in a permanent photothrombotic occlusion model of stroke. This report demonstrates the potential therapeutic benefits of using mitoNEET ligands like NL-1 as novel mitoceuticals for treating reperfusion-injury with cerebral stroke. Identifier An unambiguous reference to the resource within a given context <a href="http://doi.org/10.1007/s11095-021-03046-4" target="_blank" rel="noreferrer noopener">10.1007/s11095-021-03046-4</a> Rights Information about rights held in and over the resource Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s). Format The file format, physical medium, or dimensions of the resource journalArticle 2021 Arsiwala TA Bioenergetics Brown C CDGSH Department of Integrative Medical Sciences Geldenhuys WJ Hazlehurst LE Hollander JM Hu H Huber JD iron-sulfur journalArticle Kelley EE Lee Y May 2021 List Mdzinarishvili A NEOMED College of Medicine OXPHOS PAMPA Pharmaceutical research Pinti MV Ren X Saralkar P Simpkins J Sullivan PG t-MCAO