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40
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Text
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URL Address
<a href="http://doi.org/10.1007/s11655-016-2621-z" target="_blank" rel="noreferrer noopener">http://doi.org/10.1007/s11655-016-2621-z</a>
Pages
40–47
Issue
1
Volume
23
Dublin Core
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Title
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Salvianolate reduces murine myocardial ischemia and reperfusion injury via ERK1/2 signaling pathways in vivo.
Publisher
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Chinese journal of integrative medicine
Date
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2017
2017-01
Subject
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*MAP Kinase Signaling System/drug effects; Animal Studies; Animals; Blotting; Cardiotonic Agents/pharmacology/therapeutic use; Chinese medicine; Descriptive Statistics; extracellular signal-regulated kinase 1/2; Flavonoids/pharmacology; Heart Ventricle; Heart Ventricles/drug effects/pathology; In Vivo Studies; Inbred C57BL; ischemia and reperfusion injury; Left; Male; Mice; Mitogen-Activated Protein Kinase 1/*metabolism; Mitogen-Activated Protein Kinase 3/*metabolism; Molecular Structure; Myocardial Ischemia – Drug Therapy; Myocardial Reperfusion Injury – Drug Therapy; Myocardial Reperfusion Injury/*drug therapy/enzymology/pathology; Organ Size/drug effects; P-Value; Phosphorylation; Phosphorylation/drug effects; Plant Extracts – Administration and Dosage; Plant Extracts/chemistry/pharmacology/*therapeutic use; protein kinase B; Protein Kinase Inhibitors – Administration and Dosage; Protein Kinase Inhibitors/pharmacology; Protein Kinases – Analysis; Protein Kinases – Drug Effects; salvianolate; Signal Transduction; Staining and Labeling; Western
Creator
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Qi Jianyong; Yu Juan; Huang Dong-Hui; Guo Liheng; Wang Lei; Huang Xin; Huang Hai-Ding; Zhou Miao; Zhang Minzhou; Wu Jiashin
Description
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OBJECTIVE: To analyze the effects of salvianolate on myocardial infarction in a murine in vivo model of ischemia and reperfusion (I/R) injury. METHODS: Myocardial I/R injury model was constructed in mice by 30 min of coronary occlusion followed by 24 h of reperfusion and pretreated with salvianolate 30 min before I/R (SAL group). The SAL group was compared with SHAM (no I/R and no salvianolate), I/R (no salvianolate), and ischemia preconditioning (IPC) groups. Furthermore, an ERK1/2 inhibitor PD98059 (1 mg/kg), and a phosphatidylinositol-3-kinase (PI3-K) inhibitor, LY294002 (7.5 mg/kg), were administered intraperitoneal injection (i.p) for 30 min prior to salvianolate, followed by I/R surgery in LY and PD groups. By using a double staining method, the ratio of the infarct size (IS) to left ventricle (LV) and of risk region (RR) to LV were compared among the groups. Correlations between IS and RR were analyzed. Western-blot was used to detect the extracellular signal-regulated kinase 1/2 (ERK1/2) and protein kinase B (AKT) phosphorylation changes. RESULTS: There were no significant differences between RR to LV ratio among the SHAM, I/R, IPC and SAL groups (P\textgreater0.05). The SAL and IPC groups had IS of 26.1%+/-1.4% and 22.3%+/-2.9% of RR, respectively, both of which were significantly smaller than the I/R group (38.5%+/-2.9% of RR, P\textless0.05, P\textless0.01, respectively). Moreover, the phosphorylation of ERK1/2 was increased in SAL group (P\textless0.05), while AKT had no significant change. LY294002 further reduced IS, whereas the protective role of salvianolate could be attenuated by PD98059, which increased the IS. Additionally, the IS was not linearly related to the RR (r=0.23, 0.45, 0.62, 0.17, and 0.52 in the SHAM, I/R, SAL, LY and PD groups, respectively). CONCLUSION: Salvianolate could reduce myocardial I/R injury in mice in vivo, which involves an ERK1/2 pathway, but not a PI3-K signaling pathway.
Identifier
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<a href="http://doi.org/10.1007/s11655-016-2621-z" target="_blank" rel="noreferrer noopener">10.1007/s11655-016-2621-z</a>
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Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
*MAP Kinase Signaling System/drug effects
2017
Animal Studies
Animals
Blotting
Cardiotonic Agents/pharmacology/therapeutic use
Chinese journal of integrative medicine
Chinese medicine
Descriptive Statistics
extracellular signal-regulated kinase 1/2
Flavonoids/pharmacology
Guo Liheng
Heart Ventricle
Heart Ventricles/drug effects/pathology
Huang Dong-Hui
Huang Hai-Ding
Huang Xin
In Vivo Studies
Inbred C57BL
ischemia and reperfusion injury
Left
Male
Mice
Mitogen-Activated Protein Kinase 1/*metabolism
Mitogen-Activated Protein Kinase 3/*metabolism
Molecular Structure
Myocardial Ischemia – Drug Therapy
Myocardial Reperfusion Injury – Drug Therapy
Myocardial Reperfusion Injury/*drug therapy/enzymology/pathology
Organ Size/drug effects
P-Value
Phosphorylation
Phosphorylation/drug effects
Plant Extracts – Administration and Dosage
Plant Extracts/chemistry/pharmacology/*therapeutic use
protein kinase B
Protein Kinase Inhibitors – Administration and Dosage
Protein Kinase Inhibitors/pharmacology
Protein Kinases – Analysis
Protein Kinases – Drug Effects
Qi Jianyong
salvianolate
Signal Transduction
Staining and Labeling
Wang Lei
Western
Wu Jiashin
Yu Juan
Zhang Minzhou
Zhou Miao