1 40 1 Text A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text. URL Address <a href="http://doi.org/10.3920/BM2016.0225" target="_blank" rel="noreferrer noopener">http://doi.org/10.3920/BM2016.0225</a> Pages 71–86 Issue 1 Volume 9 Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Effect of antibiotic, probiotic, and human rotavirus infection on colonisation dynamics of defined commensal microbiota in a gnotobiotic pig model. Publisher An entity responsible for making the resource available Beneficial microbes Date A point or period of time associated with an event in the lifecycle of the resource 2018 2018-01 Subject The topic of the resource Animals; Severity of Illness Index; gut microbiota; *Germ-Free Life; Anti-Bacterial Agents/administration & dosage; Bifidobacterium longum/drug effects; Biodiversity; ciprofloxacin; Ciprofloxacin/administration & dosage/*pharmacology; Diarrhea/microbiology/physiopathology; Escherichia coli Nissle; Escherichia coli/*growth & development; Feces/microbiology; gnotobiotic pigs; Intestines/*drug effects/microbiology/pathology/physiopathology; Microbiota/*drug effects/physiology; Probiotics/administration & dosage/*pharmacology; rotavirus; Rotavirus Infections/*microbiology/physiopathology/virology; Swine; Virus Shedding/drug effects; Biological; Models; Microbial; Colony Count Creator An entity primarily responsible for making the resource Huang H-C; Vlasova A N; Kumar A; Kandasamy S; Fischer D D; Deblais L; Paim F C; Langel S N; Alhamo M A; Rauf A; Shao L; Saif L J; Rajashekara G Description An account of the resource We developed a gnotobiotic (Gn) pig model colonised with defined commensal microbiota (DMF) to provide a simplified and controlled system to study the interactions between intestinal commensals, antibiotics (ciprofloxacin, CIP), probiotics (Escherichia coli Nissle 1917, EcN) and virulent human rotavirus (VirHRV). The DMF included seven gut commensal species of porcine origin that mimic the predominant species in the infant gut. Gn piglets were divided into four groups: DMF control (non-treated), DMF+CIP (CIP treated), DMF+CIP+EcN (CIP/EcN treated), DMF+EcN (EcN treated) and inoculated orally with 10(5) cfu of each DMF strain. The pig gut was successfully colonised by all DMF species and established a simplified bacterial community by post-bacteria colonisation day (PBCD) 14/post-VirHRV challenge day (PCD) 0. Overall, Bifidobacterium adolescentis was commonly observed in faeces in all groups and time points. At PCD0, after six days of CIP treatment (DMF+CIP), we observed significantly decreased aerobic and anaerobic bacteria counts especially in jejunum (P\textless0.001), where no DMF species were detected in jejunum by T-RFLP. Following HRV challenge, 100% of pigs in DMF+CIP group developed diarrhoea with higher diarrhoea scores and duration as compared to all other groups. However, only 33% of pigs treated with EcN plus CIP developed diarrhoea. EcN treatment also enhanced the bacterial diversity and all seven DMF species were detected with a higher proportion of Bifidobacterium longum in jejunum in the DMF+CIP+EcN group on PBCD14/PCD0. Our results suggest that EcN increased the proportion of B. longum especially in jejunum and mitigated adverse impacts of antibiotic use during acute-infectious diarrhoea. The DMF model with a simplified gut commensal community can further our knowledge of how commensals and probiotics promote intestinal homeostasis and contribute to host health. Identifier An unambiguous reference to the resource within a given context <a href="http://doi.org/10.3920/BM2016.0225" target="_blank" rel="noreferrer noopener">10.3920/BM2016.0225</a> Rights Information about rights held in and over the resource Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s). *Germ-Free Life 2018 Alhamo M A Animals Anti-Bacterial Agents/administration & dosage Beneficial microbes Bifidobacterium longum/drug effects Biodiversity Biological ciprofloxacin Ciprofloxacin/administration & dosage/*pharmacology Colony Count Deblais L Diarrhea/microbiology/physiopathology Escherichia coli Nissle Escherichia coli/*growth & development Feces/microbiology Fischer D D gnotobiotic pigs gut microbiota Huang H-C Intestines/*drug effects/microbiology/pathology/physiopathology Kandasamy S Kumar A Langel S N Microbial Microbiota/*drug effects/physiology Models Paim F C Probiotics/administration & dosage/*pharmacology Rajashekara G Rauf A rotavirus Rotavirus Infections/*microbiology/physiopathology/virology Saif L J Severity of Illness Index Shao L Swine Virus Shedding/drug effects Vlasova A N