1
40
2
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Text
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URL Address
<a href="http://doi.org/10.1074/jbc.M212712200" target="_blank" rel="noreferrer noopener">http://doi.org/10.1074/jbc.M212712200</a>
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Pages
34203-34210
Issue
36
Volume
278
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Title
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Probing the binding pocket and endocytosis of a G protein-coupled receptor in live cells reported by a spin-labeled substance P agonist
Publisher
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Journal of Biological Chemistry
Date
A point or period of time associated with an event in the lifecycle of the resource
2003
2003-09
Subject
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antagonist-binding; Biochemistry & Molecular Biology; disulfide bonds; human neurokinin-1 receptor; induced; internalization; loop-gap; molecular characterization; nk-1 receptor; pathway; resonator; tachykinin nk1 receptor; xenopus oocytes
Creator
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Shafer A M; Bennett V J; Kim P; Voss J C
Description
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To probe the molecular nature of the binding pocket of a G protein-coupled receptor and the events immediately following the binding and activation, we have modified the substance P peptide, a potent agonist for the neurokinin-1 receptor, with a nitroxide spin probe specifically attached at Lys-3. The agonist properties and binding affinity of the spin-labeled substance P are similar to the native peptide. Using electron paramagnetic resonance (EPR) spectroscopy, the substance P analogue is capable of reporting the microenvironment found in the binding pocket of the receptor. The EPR spectrum of bound peptide indicates that the Lys-3 portion of the agonist is highly flexible. In addition, we detect a slight increase in the mobility of the bound peptide in the presence of a non-hydrolyzable analogue of GTP, indicative of the alternate conformational states described for this class of receptor. The down-regulation of neurokinin-tachykinin receptors is accomplished by a rapid internalization of the activated protein. Thus, it was also of interest to establish whether spin-labeled substance P could serve as a real time reporter for endocytosis. Our findings show the receptor agonist is efficiently endocytosed and the loss of EPR signal upon internalization provides a real time monitor of endocytosis. The rapid loss of signal suggests that endosomal trafficking vesicles maintain a reductive environment. Whereas the reductive capacity of the lysosome has been established, our findings indicate this capacity in early endosomes as well.
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<a href="http://doi.org/10.1074/jbc.M212712200" target="_blank" rel="noreferrer noopener">10.1074/jbc.M212712200</a>
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Journal Article
2003
antagonist-binding
Bennett V J
Biochemistry & Molecular Biology
disulfide bonds
human neurokinin-1 receptor
Induced
INTERNALIZATION
Journal Article
Journal of Biological Chemistry
Kim P
loop-gap
molecular characterization
nk-1 receptor
pathway
resonator
Shafer A M
tachykinin nk1 receptor
Voss J C
xenopus oocytes
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1111/j.1464-0597.2007.00284.x" target="_blank" rel="noreferrer noopener">http://doi.org/10.1111/j.1464-0597.2007.00284.x</a>
Pages
182–188
Issue
1
Volume
56
Dublin Core
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Title
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Internationalisation of Counseling Psychology: Constructing Cross-National Consensus and Collaboration.
Publisher
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Applied Psychology: An International Review
Date
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2007
2007-01
Subject
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PSYCHOLOGY; STRATEGIC planning; APPLIED psychology; COUNSELING; INTERNALIZATION; INTERNATIONAL Association of Applied Psychology; PERSPECTIVE (Visual perception); PSYCHOANALYSIS; SOCIAL learning
Creator
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Savickas Mark L
Description
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The SWOT analyses presented by the authors in this special issue on International Perspectives on Counseling Psychology propose numerous possibilities for building a strategic plan for the new Counseling Psychology Division (16) in the International Association of Applied Psychology. Reducing multiple possibilities to a few common themes may suggest a realistic and meaningful way forward in formulating a strategic plan for Division 16. Elements of this plan might include (a) defining counseling psychology from an international perspective, (b) crystallising a cross-national professional identity, (c) encouraging construction of indigenous models, methods, and materials, and (d) promoting international collaboration. (English) [ABSTRACT FROM AUTHOR]
Identifier
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<a href="http://doi.org/10.1111/j.1464-0597.2007.00284.x" target="_blank" rel="noreferrer noopener">10.1111/j.1464-0597.2007.00284.x</a>
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Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
2007
APPLIED psychology
Applied Psychology: An International Review
Counseling
Department of Family & Community Medicine
INTERNALIZATION
INTERNATIONAL Association of Applied Psychology
NEOMED College of Medicine
PERSPECTIVE (Visual perception)
PSYCHOANALYSIS
Psychology
Savickas Mark L
SOCIAL learning
STRATEGIC planning