1 40 1 Text A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text. URL Address <a href="http://doi.org/10.1093/nar/gkq397" target="_blank" rel="noreferrer noopener">http://doi.org/10.1093/nar/gkq397</a> Rights Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s). Pages 6007-6017 Issue 18 Volume 38 Search for Full-text Locate full-text within NEOMED Library's e-journal collections <p>Users with a NEOMED Library login can search for full-text journal articles at the following url: <a href="https://libraryguides.neomed.edu/home">https://libraryguides.neomed.edu/home</a></p> Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Genome-wide interrogation of hepatic FXR reveals an asymmetric IR-1 motif and synergy with LRH-1 Publisher An entity responsible for making the resource available Nucleic Acids Research Date A point or period of time associated with an event in the lifecycle of the resource 2010 2010-10 Subject The topic of the resource liver; orphan nuclear receptor; Biochemistry & Molecular Biology; binding; farnesoid X receptor; bile-acid; dna; activated receptor; fatty-acid synthase; ppar-gamma-rxr; target genes Creator An entity primarily responsible for making the resource Chong H K; Infante A M; Seo Y K; Jeon T I; Zhang Y Q; Edwards P A; Xie X H; Osborne T F Description An account of the resource We used mouse hepatic chromatin enriched with an FXR antibody and chromatin immunoprecipitation-sequencing (ChIP-seq) to evaluate FXR binding on a genome-wide scale. This identified 1656 FXR-binding sites and 10% were located within 2 kb of a transcription start site which is much higher than predicted by random occurrence. A motif search uncovered a canonical nuclear receptor IR-1 site, consistent with in vitro DNA-binding studies reported previously. A separate nuclear receptor half-site for monomeric receptors such as LRH-1 was co-enriched and FXR activation of four newly identified promoters was significantly augmented by an LRH-1 expression vector in a co-transfection assay. There were 1038 genes located within 20 kb of a peak and a gene set enrichment analysis showed that genes identified by our ChIP-seq analysis are highly correlated with genes activated by an FXR-VP16 adenovirus in primary mouse hepatocytes providing functional relevance to the genome-wide binding study. Gene Ontology analysis showed FXR-binding sites close to many genes in lipid, fatty acid and steroid metabolism. Other broad gene clusters related to metabolism, transport, signaling and glycolysis were also significantly enriched. Thus, FXR may have a much wider role in cellular metabolism than previously appreciated. Identifier An unambiguous reference to the resource within a given context <a href="http://doi.org/10.1093/nar/gkq397" target="_blank" rel="noreferrer noopener">10.1093/nar/gkq397</a> Format The file format, physical medium, or dimensions of the resource Journal Article or Conference Abstract Publication 2010 activated receptor bile-acid Binding Biochemistry & Molecular Biology Chong H K DNA Edwards P A Farnesoid X receptor fatty-acid synthase Infante A M Jeon T I Journal Article or Conference Abstract Publication Liver Nucleic acids research orphan nuclear receptor Osborne T F ppar-gamma-rxr Seo Y K target genes Xie X H Zhang Y Q