1
40
1
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1016/j.bbalip.2009.05.004" target="_blank" rel="noreferrer noopener">http://doi.org/10.1016/j.bbalip.2009.05.004</a>
Pages
991–996
Issue
10
Volume
1791
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Forkhead box transcription factor O1 inhibits cholesterol 7alpha-hydroxylase in human hepatocytes and in high fat diet-fed mice.
Publisher
An entity responsible for making the resource available
Biochimica et biophysica acta
Date
A point or period of time associated with an event in the lifecycle of the resource
2009
2009-10
Subject
The topic of the resource
Adenoviridae/genetics; Animals; Bile Acids and Salts/biosynthesis; Cell Line; Cell Nucleus/drug effects/metabolism; Cholesterol 7-alpha-Hydroxylase/*antagonists & inhibitors/genetics/metabolism; Dietary Fats/*administration & dosage/*pharmacology; Down-Regulation/drug effects; Enzymologic/drug effects; Feeding Behavior/*drug effects; Forkhead Box Protein O1; Forkhead Transcription Factors/genetics/*metabolism; Gene Expression Regulation; Gene Knockdown Techniques; Gene Transfer Techniques; Hepatocytes/drug effects/*enzymology; Humans; Inbred C57BL; Insulin Resistance; Insulin/metabolism; Male; Messenger/genetics/metabolism; Mice; RNA; RNA Interference/drug effects; Tumor
Creator
An entity primarily responsible for making the resource
Li Tiangang; Ma Huiyan; Park Young Joo; Lee Yoon-Kwang; Strom Stephen; Moore David D; Chiang John Y L
Description
An account of the resource
The conversion of cholesterol to bile acids is the major pathway for cholesterol catabolism. Bile acids are metabolic regulators of triglycerides and glucose metabolism in the liver. This study investigated the roles of FoxO1 in the regulation of cholesterol 7alpha-hydroxylase (CYP7A1) gene expression in primary human hepatocytes. Adenovirus-mediated expression of a phosphorylation defective and constitutively active form of FoxO1 (FoxO1-ADA) inhibited CYP7A1 mRNA expression and bile acid synthesis, while siRNA knockdown of FoxO1 resulted in a approximately 6-fold induction of CYP7A1 mRNA in human hepatocytes. Insulin caused rapid exclusion of FoxO1 from the nucleus and resulted in the induction of CYP7A1 mRNA expression, which was blocked by FoxO1-ADA. In high fat diet-fed mice, CYP7A1 mRNA expression was repressed and inversely correlated to increase hepatic FoxO1 mRNA expression and FoxO1 nuclear retention. In conclusion, our current study provides direct evidence that FoxO1 is a strong repressor of CYP7A1 gene expression and bile acid synthesis. Impaired regulation of FoxO1 may cause down-regulation of CYP7A1 gene expression and contribute to dyslipidemia in insulin resistance.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1016/j.bbalip.2009.05.004" target="_blank" rel="noreferrer noopener">10.1016/j.bbalip.2009.05.004</a>
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
2009
Adenoviridae/genetics
Animals
Bile Acids and Salts/biosynthesis
Biochimica et biophysica acta
Cell Line
Cell Nucleus/drug effects/metabolism
Chiang John Y L
Cholesterol 7-alpha-Hydroxylase/*antagonists & inhibitors/genetics/metabolism
Department of Integrative Medical Sciences
Dietary Fats/*administration & dosage/*pharmacology
Down-Regulation/drug effects
Enzymologic/drug effects
Feeding Behavior/*drug effects
Forkhead Box Protein O1
Forkhead Transcription Factors/genetics/*metabolism
Gene Expression Regulation
Gene Knockdown Techniques
Gene Transfer Techniques
Hepatocytes/drug effects/*enzymology
Humans
Inbred C57BL
Insulin Resistance
Insulin/metabolism
Lee Yoon-Kwang
Li Tiangang
Ma Huiyan
Male
Messenger/genetics/metabolism
Mice
Moore David D
NEOMED College of Medicine
Park Young Joo
RNA
RNA Interference/drug effects
Strom Stephen
Tumor