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Text
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<a href="http://doi.org/10.1080/03008207.2016.1211113" target="_blank" rel="noreferrer noopener">http://doi.org/10.1080/03008207.2016.1211113</a>
Pages
64–75
Issue
1
Volume
58
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Title
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Histone deacetylase inhibitor vorinostat (SAHA, MK0683) perturb miR-9-MCPIP1 axis to block IL-1beta-induced IL-6 expression in human OA chondrocytes.
Publisher
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Connective tissue research
Date
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2017
2017-01
Subject
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*IL-6; *MCPIP1; *miRNA; *Osteoarthritis; *Vorinostat; Adult; Aged; CCAAT-Enhancer-Binding Proteins/genetics/metabolism; Cells; Chondrocytes/*metabolism/pathology; Cultured; Female; Gene Expression Regulation/*drug effects; Genetic; Humans; Hydroxamic Acids/*pharmacology; Interleukin-1beta/genetics/*metabolism; Interleukin-6/genetics/*metabolism; Male; MicroRNAs/genetics/*metabolism; Middle Aged; Osteoarthritis/drug therapy/genetics/*metabolism/pathology; Promoter Regions; Ribonucleases/*biosynthesis/genetics; Transcription Factors/*biosynthesis/genetics; Vorinostat
Creator
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Makki Mohammad S; Haqqi Tariq M
Description
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AIM OF THE STUDY: High levels of IL-6 are believed to contribute to osteoarthritis (OA) pathogenesis. The expression of IL-6 is regulated post-transcriptionally by the miR-9-MCPIP-1 axis in chondrocytes. Vorinostat (SAHA) inhibits the IL-6 expression in OA chondrocytes. We investigated whether SAHA suppresses the expression of IL-6 by perturbing the miR-9-MCPIP1 axis in OA chondrocytes under pathological conditions. MATERIALS AND METHODS: OA chondrocytes were isolated by enzymatic digestion and treated with IL-1beta in the absence or presence of SAHA. Genes and protein expression levels were determined by TaqMan assays and Western blotting, respectively. Secreted IL-6 was quantified by enzyme linked immunosorbent assay (ELISA). MCPIP1 promoter deletion mutants were generated by polymerase chain reaction (PCR). Promoter recruitment of transcription factors was determined by ChIP. Nuclear run-on was employed to measure the ongoing transcription. siRNA-mediated knockdown of the CEBPalpha expression was employed for loss of function studies. RESULTS: Expression of MCPIP1 was high in SAHA treated OA chondrocytes but expression of IL-6 mRNAs and secreted IL-6 were reduced by \textasciitilde70%. SAHA suppressed the expression of miR-9 but enhanced the activity of the MCPIP1 promoter localized to a 156bp region which also harbors the binding site for CEBPalpha. Treatment with SAHA enhanced the recruitment of CEBPalpha to the MCPIP1 promoter. Ectopically expressed CEBPalpha enhanced the promoter activity and the expression of MCPIP1 while siRNA-mediated knockdown of CEBPalpha inhibited the expression of MCPIP1. CONCLUSIONS: Taken together our data indicate that SAHA-mediated suppression of the IL-6 expression is achieved through increased recruitment of CEBPalpha to the MCPIP1 promoter and by relieving the miR-9-mediated inhibition of MCPIP1 expression in OA chondrocytes.
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<a href="http://doi.org/10.1080/03008207.2016.1211113" target="_blank" rel="noreferrer noopener">10.1080/03008207.2016.1211113</a>
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*IL-6
*MCPIP1
*miRNA
*OSTEOARTHRITIS
*Vorinostat
2017
Adult
Aged
CCAAT-Enhancer-Binding Proteins/genetics/metabolism
Cells
Chondrocytes/*metabolism/pathology
Connective tissue research
Cultured
Department of Anatomy & Neurobiology
Female
Gene Expression Regulation/*drug effects
Genetic
Haqqi Tariq M
Humans
Hydroxamic Acids/*pharmacology
Interleukin-1beta/genetics/*metabolism
Interleukin-6/genetics/*metabolism
Makki Mohammad S
Male
MicroRNAs/genetics/*metabolism
Middle Aged
NEOMED College of Medicine
Osteoarthritis/drug therapy/genetics/*metabolism/pathology
Promoter Regions
Ribonucleases/*biosynthesis/genetics
Transcription Factors/*biosynthesis/genetics
Vorinostat