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URL Address
<a href="http://doi.org/10.1002/jcp.22704" target="_blank" rel="noreferrer noopener">http://doi.org/10.1002/jcp.22704</a>
Pages
70–76
Issue
1
Volume
227
Dublin Core
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Title
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Primary cilia regulates the directional migration and barrier integrity of endothelial cells through the modulation of hsp27 dependent actin cytoskeletal organization.
Publisher
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Journal of cellular physiology
Date
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2012
2012-01
Subject
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*Cell Movement; Actin Cytoskeleton/*metabolism; Animals; Blotting; Capillary Permeability/*physiology; Cell Adhesion; Cilia/metabolism; Endothelial Cells/cytology/*metabolism; Fluorescent Antibody Technique; Focal Adhesions/metabolism; HSP27 Heat-Shock Proteins/*metabolism; Mice; Polycystic Kidney Diseases/physiopathology; Signal Transduction/physiology; Transgenic; Western
Creator
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Jones Thomas J; Adapala Ravi K; Geldenhuys Werner J; Bursley Chris; AbouAlaiwi Wissam A; Nauli Surya M; Thodeti Charles K
Description
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Cilia are mechanosensing organelles that communicate extracellular signals into intracellular responses. Altered functions of primary cilia play a key role in the development of various diseases including polycystic kidney disease. Here, we show that endothelial cells from the oak ridge polycystic kidney (Tg737(orpk/orpk) ) mouse, with impaired cilia assembly, exhibit a reduction in the actin stress fibers and focal adhesions compared to wild-type (WT). In contrast, endothelial cells from polycystin-1 deficient mice (pkd1(null/null) ), with impaired cilia function, display robust stress fibers, and focal adhesion assembly. We found that the Tg737(orpk/orpk) cells exhibit impaired directional migration and endothelial cell monolayer permeability compared to the WT and pkd1(null/null) cells. Finally, we found that the expression of heat shock protein 27 (hsp27) and the phosphorylation of focal adhesion kinase (FAK) are downregulated in the Tg737(orpk/orpk) cells and overexpression of hsp27 restored both FAK phosphorylation and cell migration. Taken together, these results demonstrate that disruption of the primary cilia structure or function compromises the endothelium through the suppression of hsp27 dependent actin organization and focal adhesion formation, which may contribute to the vascular dysfunction in ciliopathies.
Identifier
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<a href="http://doi.org/10.1002/jcp.22704" target="_blank" rel="noreferrer noopener">10.1002/jcp.22704</a>
Rights
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Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
*Cell Movement
2012
AbouAlaiwi Wissam A
Actin Cytoskeleton/*metabolism
Adapala Ravi K
Animals
Blotting
Bursley Chris
Capillary Permeability/*physiology
Cell Adhesion
Cilia/metabolism
Department of Integrative Medical Sciences
Endothelial Cells/cytology/*metabolism
Fluorescent Antibody Technique
Focal Adhesions/metabolism
Geldenhuys Werner J
HSP27 Heat-Shock Proteins/*metabolism
Jones Thomas J
Journal of cellular physiology
Mice
Nauli Surya M
NEOMED College of Medicine
Polycystic Kidney Diseases/physiopathology
Signal Transduction/physiology
Thodeti Charles K
Transgenic
Western