1 40 1 Text A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text. URL Address <a href="http://doi.org/10.1117/1.1922329" target="_blank" rel="noreferrer noopener">http://doi.org/10.1117/1.1922329</a> Rights Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s). Pages 6-6 Issue 3 Volume 10 Search for Full-text Locate full-text within NEOMED Library's e-journal collections <p>Users with a NEOMED Library login can search for full-text journal articles at the following url: <a href="https://libraryguides.neomed.edu/home">https://libraryguides.neomed.edu/home</a></p> Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Fourier Transform Infrared Imaging Spectroscopic Analysis Of Tissue Engineered Cartilage: Histologic And Biochemical Correlations Publisher An entity responsible for making the resource available Journal of Biomedical Optics Date A point or period of time associated with an event in the lifecycle of the resource 2005 2005-05 Subject The topic of the resource Biochemistry & Molecular Biology; bioreactor; chondrocytes; collagen; Fourier transform infrared imaging spectroscopy; hollow fiber bioreactor; hollow-fiber; human articular-cartilage; magnetic-resonance microscopy; matrix; Medical Imaging; model; mri techniques; Nuclear Medicine &; Optics; osteoarthritis; proteoglycans; Radiology; tissue engineered cartilage; tissue engineering Creator An entity primarily responsible for making the resource Kim M; Bi X H; Horton W E; Spencer R G; Camacho N P Description An account of the resource The composition of cartilage is predictive of its in vivo performance. Therefore, the ability to assess its primary macromolecular components, proteoglycan (PG) and collagen, is of great importance. In the current study, we hypothesized that PG content and distribution in tissue engineered cartilage could be determined using Fourier-transform infrared imaging spectroscopy (FT-IRIS). The cartilage was grown from chondrocytes within a hollow fiber bioreactor (HFBR) system previously used extensively to study cartilage development. FT-IRIS analysis showed a gradient of PG content, with the highest content in the center near the nutritive fibers and the lowest near the interior surface of the HFBR. Further, we found significantly greater PG content in the region near culture medium inflow (45.0%) as compared to the outflow region (24.7%) (p < 0.001). This difference paralleled the biochemically determined glycosaminoglycan difference of 42.6% versus 27.8%. In addition, FT-IRIS-determined PG content at specific positions within the tissue sections correlated with histologically determined PG content (R=50.73, p=50.007). In summary, FT-IRIS determination of PG correlates with histological determination of PG and yields quantitatively similar results to biochemical determination of glycosaminoglycan in developing cartilage. (c) 2005 Society of Photo-Optical Instrumentation Engineers. Identifier An unambiguous reference to the resource within a given context <a href="http://doi.org/10.1117/1.1922329" target="_blank" rel="noreferrer noopener">10.1117/1.1922329</a> Format The file format, physical medium, or dimensions of the resource Journal Article or Conference Abstract Publication 2005 Bi X H Biochemistry & Molecular Biology bioreactor Camacho N P Chondrocytes Collagen Fourier transform infrared imaging spectroscopy hollow fiber bioreactor hollow-fiber Horton W E human articular-cartilage Journal Article or Conference Abstract Publication Journal of Biomedical Optics Kim M magnetic-resonance microscopy matrix Medical Imaging model mri techniques Nuclear Medicine & optics Osteoarthritis proteoglycans Radiology Spencer R G tissue engineered cartilage Tissue Engineering