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Text
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URL Address
<a href="http://doi.org/10.1093/chromsci/bmr055" target="_blank" rel="noreferrer noopener">http://doi.org/10.1093/chromsci/bmr055</a>
Pages
271–276
Issue
3
Volume
50
Dublin Core
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Title
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Development and validation of a novel RP-HPLC method for the analysis of reduced glutathione.
Publisher
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Journal of chromatographic science
Date
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2012
2012-03
Subject
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Chromatography; Glutathione/*analysis; High Pressure Liquid/*methods; Limit of Detection; Linear Models; Nanoparticles/chemistry; Pharmaceutical Preparations/chemistry; Reproducibility of Results; Reverse-Phase/*methods; Spectrophotometry; Ultraviolet
Creator
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Sutariya Vijaykumar; Wehrung Daniel; Geldenhuys Werner J
Description
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The objective of this study was the development, optimization, and validation of a novel reverse-phase high-pressure liquid chromatography (RP-HPLC) method for the quantification of reduced glutathione in pharmaceutical formulations utilizing simple UV detection. The separation utilized a C18 column at room temperature and UV absorption was measured at 215 nm. The mobile phase was an isocratic flow of a 50/50 (v/v) mixture of water (pH 7.0) and acetonitrile flowing at 1.0 mL/min. Validation of the method assessed the methods ability in seven categories: linearity, range, limit of detection, limit of quantification, accuracy, precision, and selectivity. Analysis of the system suitability showed acceptable levels of suitability in all categories. Likewise, the method displayed an acceptable degree of linearity (r(2) = 0.9994) over a concentration range of 2.5-60 microg/mL. The detection limit and quantification limit were 0.6 and 1.8 microg/mL respectively. The percent recovery of the method was 98.80-100.79%. Following validation the method was employed in the determination of glutathione in pharmaceutical formulations in the form of a conjugate and a nanoparticle. The proposed method offers a simple, accurate, and inexpensive way to quantify reduced glutathione.
Identifier
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<a href="http://doi.org/10.1093/chromsci/bmr055" target="_blank" rel="noreferrer noopener">10.1093/chromsci/bmr055</a>
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Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
2012
Chromatography
Geldenhuys Werner J
Glutathione/*analysis
High Pressure Liquid/*methods
Journal of chromatographic science
Limit of Detection
Linear Models
Nanoparticles/chemistry
Pharmaceutical Preparations/chemistry
Reproducibility of Results
Reverse-Phase/*methods
Spectrophotometry
Sutariya Vijaykumar
Ultraviolet
Wehrung Daniel