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40
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Text
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URL Address
<a href="http://doi.org/10.1016/j.orthres.2004.12.016" target="_blank" rel="noreferrer noopener">http://doi.org/10.1016/j.orthres.2004.12.016</a>
Pages
1128–1138
Issue
5
Volume
23
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Intrajoint comparisons of gene expression patterns in human osteoarthritis suggest a change in chondrocyte phenotype.
Publisher
An entity responsible for making the resource available
Journal of orthopaedic research : official publication of the Orthopaedic Research Society
Date
A point or period of time associated with an event in the lifecycle of the resource
2005
2005-09
Subject
The topic of the resource
*Gene Expression Profiling; 80 and over; Aged; Articular/*metabolism; Cartilage; Chondrocytes/*metabolism; Collagen Type II/genetics; Glycosaminoglycans/analysis; High Mobility Group Proteins/genetics; Humans; Matrix Metalloproteinase 3/genetics; Matrix Metalloproteinase 9/genetics; Messenger/analysis; Osteoarthritis/*metabolism; Phenotype; Proto-Oncogene Proteins c-bcl-2/genetics; RNA; SOX9 Transcription Factor; Transcription Factors/genetics
Creator
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Yagi Rieko; McBurney Denise; Laverty David; Weiner Scott; Horton Walter E Jr
Description
An account of the resource
Osteoarthritis (OA) is a degenerative cartilage disease with varying degrees of severity within a given joint. The purpose of this study was to define a sampling procedure for comparing human minimal and advanced OA cartilage in the same patient and to determine basic patterns of gene expression in these regions. A specific hypothesis under study was that the expression level of Bcl-2 would correlate with Sox9 and aggrecan mRNA expression in vivo as has been demonstrated in vitro. Femoral condylar advanced OA cartilage was located within 1cm of overt lesions, and minimal cartilage was taken from areas with no obvious surface defects. Histological sections were scored for disease severity and chondroitin sulfate and hydroxyproline content was determined. The expression level of nine specific genes (aggrecan, collagen type II, Bcl-2, Sox9, Link protein, osteopontin, and MMP-13, -3, and -9) was determined by quantitative real time PCR. The scores for fibrillation, chondrocyte cloning, and proteoglycan depletion were significantly different between advanced and minimal OA cartilage. The advanced OA cartilage had significantly less chondroitin sulfate than the minimal OA cartilage. Osteopontin mRNA expression showed a 3.6-fold increase in advanced compared to minimal OA cartilage. In contrast, the level of mRNA coding for aggrecan, link protein, Bcl-2, Sox9 and MMP-3, -9, -13 were all decreased in advanced compared to minimal cartilage in the majority of the patients studied. Collagen type II mRNA expression displayed a wide-range of variation. A statistically significant correlation was observed both between Bcl-2 and Sox9 mRNA level, and between Bcl-2 and aggrecan mRNA expression. The patient matched comparison of minimal and advanced OA cartilage revealed differences in cellular and tissue characteristics, and changes in gene expression that may be involved in OA progression. In addition, Bcl-2 may also play a role in regulating the expression of aggrecan through Sox9 in vivo as well as in vitro.
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An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1016/j.orthres.2004.12.016" target="_blank" rel="noreferrer noopener">10.1016/j.orthres.2004.12.016</a>
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
*Gene Expression Profiling
2005
80 and over
Aged
Articular/*metabolism
Cartilage
Chondrocytes/*metabolism
Collagen Type II/genetics
Department of Anatomy & Neurobiology
Glycosaminoglycans/analysis
High Mobility Group Proteins/genetics
Horton Walter E Jr
Humans
Journal of orthopaedic research : official publication of the Orthopaedic Research Society
Laverty David
Matrix Metalloproteinase 3/genetics
Matrix Metalloproteinase 9/genetics
McBurney Denise
Messenger/analysis
NEOMED College of Medicine
Osteoarthritis/*metabolism
Phenotype
Proto-Oncogene Proteins c-bcl-2/genetics
RNA
SOX9 Transcription Factor
Transcription Factors/genetics
Weiner Scott
Yagi Rieko
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1002/jor.23262" target="_blank" rel="noreferrer noopener">http://doi.org/10.1002/jor.23262</a>
Pages
311–320
Issue
2
Volume
35
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Harpagoside suppresses IL-6 expression in primary human osteoarthritis chondrocytes.
Publisher
An entity responsible for making the resource available
Journal of orthopaedic research : official publication of the Orthopaedic Research Society
Date
A point or period of time associated with an event in the lifecycle of the resource
2017
2017-02
Subject
The topic of the resource
*c-FOS/AP-1; *harpagoside; *IL-6; *MMP-13; *osteoarthritis; CCAAT-Enhancer-Binding Protein-beta/metabolism; Chemokines/metabolism; Chondrocytes/*drug effects/metabolism; Drug Evaluation; Glycosides/pharmacology/*therapeutic use; Harpagophytum; Humans; Interleukin-1beta; Interleukin-6/antagonists & inhibitors/*metabolism; Matrix Metalloproteinase 13/metabolism; NF-kappa B/metabolism; Osteoarthritis/*drug therapy/metabolism; Phytotherapy; Plant Extracts/pharmacology/therapeutic use; Preclinical; Primary Cell Culture; Proto-Oncogene Proteins c-fos/metabolism; Pyrans/pharmacology/*therapeutic use; Reactive Oxygen Species/metabolism; Transcription Factor AP-1/metabolism
Creator
An entity primarily responsible for making the resource
Haseeb Abdul; Ansari Mohammad Yunus; Haqqi Tariq M
Description
An account of the resource
There is growing evidence in support of the involvement of inflammatory response in the pathogenesis of osteoarthritis (OA). Harpagoside, one of the bioactive components of Harpagophytum procumbens (Hp), has been shown to possess anti-inflammatory properties. Here we used an in vitro model of inflammation in OA to investigate the potential of harpagoside to suppress the production of inflammatory cytokines/chemokines such as IL-6 and matrix degrading proteases. We further investigated the likely targets of harpagoside in primary human OA chondrocytes. OA chondrocytes were pre-treated with harpagoside before stimulation with IL-1beta. mRNA expression profile of 92 cytokines/chemokines was determined using TaqMan Human Chemokine PCR Array. Expression levels of selected mRNAs were confirmed using TaqMan assays. Protein levels of IL-6 and MMP-13 were assayed by ELISA and immunoblotting. Total protein levels and phosphorylation of signaling proteins were determined by immunoblotting. Cellular localization of
Identifier
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<a href="http://doi.org/10.1002/jor.23262" target="_blank" rel="noreferrer noopener">10.1002/jor.23262</a>
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
*c-FOS/AP-1
*harpagoside
*IL-6
*MMP-13
*OSTEOARTHRITIS
2017
Ansari Mohammad Yunus
CCAAT-Enhancer-Binding Protein-beta/metabolism
Chemokines/metabolism
Chondrocytes/*drug effects/metabolism
Department of Anatomy & Neurobiology
Drug Evaluation
Glycosides/pharmacology/*therapeutic use
Haqqi Tariq M
Harpagophytum
Haseeb Abdul
Humans
Interleukin-1beta
Interleukin-6/antagonists & inhibitors/*metabolism
Journal of orthopaedic research : official publication of the Orthopaedic Research Society
Matrix Metalloproteinase 13/metabolism
NEOMED College of Medicine
NF-kappa B/metabolism
Osteoarthritis/*drug therapy/metabolism
Phytotherapy
Plant Extracts/pharmacology/therapeutic use
Preclinical
Primary Cell Culture
Proto-Oncogene Proteins c-fos/metabolism
Pyrans/pharmacology/*therapeutic use
Reactive Oxygen Species/metabolism
Transcription Factor AP-1/metabolism