Browse Items · NEOMED Bibliography Database

Relationships between Very Low-Density Lipoproteins-Ceramides, -Diacylglycerols, and -Triacylglycerols in Insulin-Resistant Men.

This short report describes the relationships between concentrations of ceramides (CER), diacylglycerols (DAG), triacylglycerols (TAG) in very low-density lipoproteins (VLDL) particles, and hepatic lipid accumulation. VLDL particles were isolated…

Temporal Dynamics of High-Density Lipoprotein Proteome in Diet-Controlled Subjects with Type 2 Diabetes.

We examined the effect of mild hyperglycemia on high-density lipoprotein (HDL) metabolism and kinetics in diet-controlled subjects with type 2 diabetes (T2D). (2)H2O-labeling coupled with mass spectrometry was applied to quantify HDL cholesterol…

Tags: 2020, Apolipoproteins, Biomolecules, Department of Pharmaceutical Sciences, HDL dysfunction, Heavy water, Ilchenko Serguei, Journal Article, journalArticle, June 2020 Update I, Kashyap Sangeeta R, Kasumov Takhar, Kheniser Karim G, Kim Chunki, NEOMED College of Pharmacy, Osme Abdullah, proteomics, Type 2 diabetes

Calculation of the Protein Turnover Rate Using the Number of Incorporated 2H Atoms and Proteomics Analysis of a Single Labeled Sample

Rate constant estimation with heavy water requires a long-term experiment with data collection at multiple time points (3-4 weeks for mitochondrial proteome dynamics in mice and much longer in other species). When tissue proteins are analyzed, this…

Tags: 2019, Analytical Chemistry, Department of Pharmaceutical Sciences, Department of Pharmacy Practice, Haddad Andrew, Ilchenko Serguei, Journal Article, Kasumov Takhar, NEOMED College of Graduate Studies, NEOMED College of Pharmacy, November 2019 Update, Recchia Fabio A, Sadana Prabodh, Sadygov Rovshan G

HDL Flux is Higher in Patients with Nonalcoholic Fatty Liver Disease

Altered lipid metabolism and inflammation are involved in the pathogenesis of both non-alcoholic fatty liver disease (NAFLD) and cardiovascular disease (CVD). Even though high-density lipoprotein (HDL), a CVD protective marker, is decreased, whether…

Lipocalin‐2 Protects Against Diet‐Induced Nonalcoholic Fatty Liver Disease by Targeting Hepatocytes.

Hepatocytes are the major source of hepatic lipocalin‐2 (LCN2), which is up‐regulated in response to inflammation, injury, or metabolic stress. So far, the role of hepatocyte‐derived LCN2 in the development of nonalcoholic fatty liver disease (NAFLD)…

Tags: 2019, Chen Xiaoli, Department of Integrative Medical Sciences, Fatty Liver, Hepatology communications, Jadhav Kavita, Kasumov Takhar, Li Yuanyuan, LIPOCALIN, LIVER disease treatment, NEOMED College of Medicine, September 2019 Update, Sun Huihui, Xu Yanyong, Yin Liya, Zhang Yanqiao, Zhu Yingdong

A novel regulatory role of TRAPPC9 in L-plastin-mediated osteoclast actin ring formation

Trafficking protein particle complex 9 (TRAPPC9) is a major subunit of the TRAPPII complex. TRAPPC9 has been reported to bind nuclear factor κB kinase subunit β (IKKβ) and NF-kB-inducing kinase (NIK) where it plays a role in the canonical and…

Reversal of metabolic disorders by pharmacological activation of bile acid receptors TGR5 and FXR.

OBJECTIVES: Activation of the bile acid (BA) receptors farnesoid X receptor (FXR) or G protein-coupled bile acid receptor (GPBAR1; TGR5) improves metabolic homeostasis. In this study, we aim to determine the impact of pharmacological activation of…

Glycation Reduces the Stability of ApoAI and Increases HDL Dysfunction in Diet-Controlled Type 2 Diabetes.

Context: Hyperglycemia plays a key role in the pathogenesis of cardiovascular complications of diabetes. Type 2 diabetes mellitus (T2DM) is associated with high-density lipoprotein (HDL) dysfunction and increased degradation of apolipoprotein I…

Tags: 2018, Adult, Aged, Animal Studies, Animals, Apolipoprotein A-I/blood/*metabolism, Apolipoproteins – Blood, Apolipoproteins – Metabolism, Bena James, Biochemical Phenomena, Case Control Studies, Case-Control Studies, Cells, Comparative Studies, Cultured, Department of Pharmaceutical Sciences, Diabetes Mellitus, Diet, Dyslipidemias/complications/diet therapy/*metabolism, Evaluation Research, Female, Funding Source, Glycosylation, Golizeh Makan, HDL – Metabolism, HDL/*metabolism, Human, Humans, Hyperglycemia – Complications, Hyperglycemia – Diet Therapy, Hyperglycemia – Metabolism, Hyperglycemia/complications/diet therapy/*metabolism, Hyperlipidemia – Complications, Hyperlipidemia – Diet Therapy, Hyperlipidemia – Metabolism, Ilchenko Serguei, Kashyap Sangeeta R, Kasumov Takhar, Lee Kwangwon, Lipoproteins, Male, Mice, Middle Age, Middle Aged, Multicenter Studies, NEOMED College of Pharmacy, Osme Abdullah, Previs Stephen F, Protein Stability, Smith Jonathan D, The Journal of clinical endocrinology and metabolism, Type 2 – Complications, Type 2 – Diet Therapy, Type 2 – Metabolism, Type 2/complications/*diet therapy/metabolism, Validation Studies, Wang Shuhui

Turnover of histones and histone variants in postnatal rat brain: effects of alcohol exposure.

BACKGROUND: Alcohol consumption during pregnancy is a significant public health problem and can result in a continuum of adverse outcomes to the fetus known as fetal alcohol spectrum disorders (FASD). Subjects with FASD show significant neurological…

Erratum to: The therapeutic efficacy of intensive medical therapy in ameliorating high-density lipoprotein dysfunction in subjects with type two diabetes.

Tags: 2016, Bena James, Department of Pharmaceutical Sciences, Kashyap Sangeeta, Kasumov Takhar, Kheniser Karim, Li Ling, Lipids in health and disease, NEOMED College of Pharmacy

The therapeutic efficacy of intensive medical therapy in ameliorating high-density lipoprotein dysfunction in subjects with type two diabetes.

BACKGROUND: To determine whether 12 months of intensive medical therapy (IMT) improves HDL functionality parameters in subjects with type II diabetes (T2D). METHODS: Retrospective, randomized, and controlled 12-month IMT intervention trial that…

Quantifying ceramide kinetics in vivo using stable isotope tracers and LC-MS/MS.

Numerous studies have implicated dyslipidemia as a key factor in mediating insulin resistance. Ceramides have received special attention since their levels are inversely associated with normal insulin signaling and positively associated with factors…

Enhancing Studies of Pharmacodynamic Mechanisms via Measurements of Metabolic Flux: Fundamental Concepts and Guiding Principles for Using Stable Isotope Tracers.

Drug discovery and development efforts are largely based around a common expectation, namely, that direct or indirect action on a cellular process (e.g., statin-mediated enzyme inhibition or insulin-stimulated receptor activation) will have a…

Hyperammonaemia-induced skeletal muscle mitochondrial dysfunction results in cataplerosis and oxidative stress.

KEY POINTS: Hyperammonaemia occurs in hepatic, cardiac and pulmonary diseases with increased muscle concentration of ammonia. We found that ammonia results in reduced skeletal muscle mitochondrial respiration, electron transport chain complex I…

Tags: *ammonia, *ATP, *cellular respiration, *cirrhosis, *Mitochondria, *Oxidative Stress, *portacaval anastamosis, *Reactive oxygen species, *skeletal muscle, 2016, Adenosine Triphosphate/metabolism, Aged, Allawy Allawy, Animals, Cell Line, Cell Respiration, Creatine Kinase/metabolism, Dasarathy Srinivasan, Davuluri Gangarao, Department of Pharmaceutical Sciences, Female, Flask Chris A, Hoppel Charles, Humans, Hyperammonemia/*metabolism, Kasumov Takhar, Kumar Avinash, Liver Cirrhosis/metabolism, Male, Middle Aged, Mitochondria, Muscle, Muscle/*metabolism, Myosin Heavy Chains/metabolism, NAD/metabolism, NEOMED College of Pharmacy, Rats, Reactive Oxygen Species/metabolism, Rennison Julie H, Sandlers Yana, Singh Dharmvir, Skeletal/*metabolism, Sprague-Dawley, Thapaliya Samjhana, The Journal of physiology, Thiobarbituric Acid Reactive Substances/metabolism, Van Wagoner David R

Monitoring the synthesis of biomolecules using mass spectrometry.

The controlled and selective synthesis/clearance of biomolecules is critical for most cellular processes. In most high-throughput 'omics' studies, we measure the static quantities of only one class of biomolecules (e.g. DNA, mRNA, proteins or…

Tags: 2016, biosynthesis, Department of Pharmaceutical Sciences, Deuterium Oxide/chemistry, deuterium water, flux analysis, Kasumov Takhar, Kinetics, Macromolecular Substances/*chemistry/*metabolism, Mass spectrometry, Mass Spectrometry/*methods, Metabolomics, Miyagi Masaru, NEOMED College of Pharmacy, Philosophical transactions. Series A, Mathematical, physical, and engineering sciences, proteomics

Hepatic Mitochondrial Defects in a Nonalcoholic Fatty Liver Disease Mouse Model Are Associated with Increased Degradation of Oxidative Phosphorylation Subunits.

Nonalcoholic fatty liver disease (NAFLD) is associated with hepatic mitochondrial dysfunction characterized by reduced ATP synthesis. We applied the (2)H2O-metabolic labeling approach to test the hypothesis that the reduced stability of oxidative…

d2ome, Software for in Vivo Protein Turnover Analysis Using Heavy Water Labeling and LC-MS, Reveals Alterations of Hepatic Proteome Dynamics in a Mouse Model of NAFLD.

Metabolic labeling with heavy water followed by LC-MS is a high throughput approach to study proteostasis in vivo. Advances in mass spectrometry and sample processing have allowed consistent detection of thousands of proteins at multiple time points.…

Gaussian Process Modeling of Protein Turnover.

We describe a stochastic model to compute in vivo protein turnover rate constants from stable-isotope labeling and high-throughput liquid chromatography-mass spectrometry experiments. We show that the often-used one- and two-compartment nonstochastic…

Stable isotope-based flux studies in nonalcoholic fatty liver disease.

Nonalcoholic fatty liver disease (NAFLD) is the most common chronic liver disease and is associated with the worldwide epidemics of obesity, diabetes and cardiovascular diseases. NAFLD ranges from benign fat accumulation in the liver (steatosis) to…

Tags: *Citric acid cycle, *Fatty acid oxidation, *Fibrosis, *NAFLD, *Oxidative Stress, *Stable isotopes, 2018, Animals, Department of Pharmaceutical Sciences, Humans, Isotopes/metabolism, Kasumov Takhar, Mass Spectrometry/*methods, McCullough Arthur, NEOMED College of Pharmacy, Non-alcoholic Fatty Liver Disease/*metabolism, Pharmacology & therapeutics, Previs Stephen

Reversal of metabolic disorders by pharmacological activation of bile acid receptors TGR5 and FXR.

OBJECTIVES: Activation of the bile acid (BA) receptors farnesoid X receptor (FXR) or G protein-coupled bile acid receptor (GPBAR1; TGR5) improves metabolic homeostasis. In this study, we aim to determine the impact of pharmacological activation of…

Increased serotransferrin and ceruloplasmin turnover in diet-controlled patients with type 2 diabetes.

Type 2 diabetes mellitus (T2DM) is associated with oxidative stress and perturbed iron metabolism. Serotransferrin (Trf) and ceruloplasmin (Cp) are two key proteins involved in iron metabolism and anti-oxidant defense. Non-enzymatic glycation and…

Tags: *Ceruloplasmin, *Deamidation, *Heavy water metabolic labeling, *High resolution mass spectrometry, *Iron metabolism, *LC-MS/MS, *Non-enzymatic glycation, *Oxidative Stress, *Protein Processing, *Proteome dynamics, *Serotransferrin, *Type 2 diabetes mellitus, 2017, Adult, Amino Acid Sequence, Bena James, Case-Control Studies, Ceruloplasmin/genetics/*metabolism, Department of Pharmaceutical Sciences, Deuterium/metabolism, Diabetes Mellitus, Diabetic, Diet, Female, Free radical biology & medicine, Gene Expression Regulation, Glycated Hemoglobin A/genetics/metabolism, Glycosylation, Golizeh Makan, Humans, Ilchenko Serguei, Iron/*metabolism, Isotope Labeling, Kashyap Sangeeta R, Kasumov Takhar, Lee Kwangwon, Male, Middle Aged, NEOMED College of Pharmacy, Osme Abdullah, Oxidation-Reduction, Oxidative Stress, Post-Translational, Proteolysis, Sadygov Rovshan G, Transferrin/genetics/*metabolism, Type 2/diet therapy/genetics/*metabolism/pathology

A Western diet induced NAFLD in LDLR(-/)(-) mice is associated with reduced hepatic glutathione synthesis.

Oxidative stress plays a key role in the pathogenesis of non-alcoholic fatty liver disease (NAFLD). Glutathione is the major anti-oxidant involved in cellular oxidative defense, however there are currently no simple non-invasive methods for assessing…

Carboxylesterase 2 prevents liver steatosis by modulating lipolysis, endoplasmic reticulum stress, and lipogenesis and is regulated by hepatocyte nuclear factor 4 alpha in mice.

UNLABELLED: Nonalcoholic fatty liver disease (NAFLD) is a common liver disease that ranges from simple steatosis to nonalcoholic steatohepatitis (NASH). So far, the underlying mechanism remains poorly understood. Here, we show that hepatic…

Tags: *Lipid Metabolism, 2016, Adiposity, Animals, Carboxylesterase/*metabolism, Carboxylic Ester Hydrolases/genetics/*metabolism, Department of Integrative Medical Sciences, Department of Pharmaceutical Sciences, Diabetes Mellitus, Diet, Endoplasmic Reticulum Stress, Energy Metabolism, Experimental/enzymology, Gene Knockdown Techniques, Glucose Tolerance Test, Glucose/metabolism, Hepatocyte Nuclear Factor 4/*metabolism, Hepatology (Baltimore, Md.), High-Fat/adverse effects, Homeostasis, Humans, Inbred C57BL, Jadhav Kavita, Kasumov Takhar, Li Yuanyuan, Lipogenesis, Lipolysis, Liver/enzymology, Male, Mice, NEOMED College of Medicine, NEOMED College of Pharmacy, Non-alcoholic Fatty Liver Disease/*etiology/metabolism, Obesity/enzymology/etiology, Sterol Regulatory Element Binding Protein 1/metabolism, Xu Yang, Yin Liya, Zalzala Munaf, Zhang Yanqiao

Browse Items (23 total)