1
40
6
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1016/s0016-5085(00)86175-2" target="_blank" rel="noreferrer noopener">http://doi.org/10.1016/s0016-5085(00)86175-2</a>
Rights
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
Pages
A1006-A1006
Issue
4
Volume
118
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Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Bile acids and FXR represses cholesterol 7A-hydroxylase (CYP7A1), sterol 12A-hydroxylase (CYP8B1) and sterol 27-hydroxylase (CYP27A1), but not oxysterol 7A-hydroxylase (CYP7B1) gene transcription
Publisher
An entity responsible for making the resource available
Gastroenterology
Date
A point or period of time associated with an event in the lifecycle of the resource
2000
2000-04
Subject
The topic of the resource
Gastroenterology & Hepatology
Creator
An entity primarily responsible for making the resource
Chiang J Y; Chen W; Zheng M; Wu Z; Kimmel R; Stroup D
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1016/s0016-5085(00)86175-2" target="_blank" rel="noreferrer noopener">10.1016/s0016-5085(00)86175-2</a>
Format
The file format, physical medium, or dimensions of the resource
Journal Article or Conference Abstract Publication
2000
Chen W
Chiang J Y
Gastroenterology
Gastroenterology & Hepatology
Journal Article or Conference Abstract Publication
Kimmel R
Stroup D
Wu Z
Zheng M
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
n/a
Rights
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
Pages
319A-319A
Issue
4
Volume
30
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Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Farnesoid X receptor (FXR) is a bile acid receptor that mediates transcriptional regulation of the cholesterol 7 alpha-hydroxylase gene (CYP7A1) by bile acids
Publisher
An entity responsible for making the resource available
Hepatology
Date
A point or period of time associated with an event in the lifecycle of the resource
1999
1999-10
Subject
The topic of the resource
Gastroenterology & Hepatology
Creator
An entity primarily responsible for making the resource
Chiang J Y; Kimmel R; Weinberger C; Stroup D
Identifier
An unambiguous reference to the resource within a given context
n/a
Format
The file format, physical medium, or dimensions of the resource
Journal Article or Conference Abstract Publication
1999
Chiang J Y
Gastroenterology & Hepatology
Hepatology
Journal Article or Conference Abstract Publication
Kimmel R
Stroup D
Weinberger C
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1074/jbc.275.15.10918" target="_blank" rel="noreferrer noopener">http://doi.org/10.1074/jbc.275.15.10918</a>
Rights
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
Pages
10918-10924
Issue
15
Volume
275
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Title
A name given to the resource
Farnesoid X receptor responds to bile acids and represses cholesterol 7 alpha-hydroxylase gene (CYP7A1) transcription
Publisher
An entity responsible for making the resource available
Journal of Biological Chemistry
Date
A point or period of time associated with an event in the lifecycle of the resource
2000
2000-04
Subject
The topic of the resource
orphan nuclear receptor; Biochemistry & Molecular Biology; expression; pathway; activation; identification; promoter; element; metabolites; hepg2 cells; ligands
Creator
An entity primarily responsible for making the resource
Chiang J Y L; Kimmel R; Weinberger C; Stroup D
Description
An account of the resource
Cholesterol 7 alpha-hydroxylase gene (CYP7A1) transcription is repressed by bile acids. The goal of this study is to elucidate the mechanism of CYP7A1 transcription by bile acid-activated farnesoid X receptor (FXR) in its native promoter and cellular context and to identify FXR response elements in the gene. In Chinese hamster ovary cells transfected with retinoid X receptor alpha (RXR alpha)/FXR, only chenodeoxycholic acid (CDCA) and deoxycholic acid (DCA) were able to stimulate a heterologous promoter/reporter containing an ecdysone response element. In HepG2 cells, all bile acids (25 mu M) were able to repress CYP7A1/luciferase reporter activity, and only CDCA and DCA further repressed reporter activity when cotransfected with RXR alpha/FXR, The concentration of CDCA required to inhibit 50% of reporter activity (IC(50)) was determined to be approximately 25 mu M without FXR and 10 mu M with FXR. Deletion analysis revealed that the bile acid response element located between nucleotides -148 and -128 was the FXR response element, but RXR alpha/FXR did not bind to this sequence. These results suggest that bile acid-activated FXR exerts its inhibitory effect on CYP7A1 transcription by an indirect mechanism, in contrast to the stimulation and binding of FXR to intestinal bile acid-binding protein gene promoter. Results also reveal that bile acid receptors other than FXR are present in HepG2 cells.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1074/jbc.275.15.10918" target="_blank" rel="noreferrer noopener">10.1074/jbc.275.15.10918</a>
Format
The file format, physical medium, or dimensions of the resource
Journal Article or Conference Abstract Publication
2000
activation
Biochemistry & Molecular Biology
Chiang J Y L
element
expression
hepg2 cells
identification
Journal Article or Conference Abstract Publication
Journal of Biological Chemistry
Kimmel R
Ligands
metabolites
orphan nuclear receptor
pathway
promoter
Stroup D
Weinberger C
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
Pages
10918–10924
Issue
15
Volume
275
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Farnesoid X receptor responds to bile acids and represses cholesterol 7alpha-hydroxylase gene (CYP7A1) transcription.
Publisher
An entity responsible for making the resource available
The Journal of biological chemistry
Date
A point or period of time associated with an event in the lifecycle of the resource
2000
2000-04
Subject
The topic of the resource
Humans; Animals; Cricetinae; Cholesterol 7-alpha-Hydroxylase/*genetics; Response Elements; Bile Acids and Salts/*pharmacology; Transcription Factors/genetics/*physiology; Retinoid X Receptors; DNA-Binding Proteins/*physiology; Repressor Proteins/*physiology; Cultured; Receptors; Genetic; Tumor Cells; Promoter Regions; *Transcription; Cytoplasmic and Nuclear; Retinoic Acid/genetics
Creator
An entity primarily responsible for making the resource
Chiang J Y; Kimmel R; Weinberger C; Stroup D
Description
An account of the resource
Cholesterol 7alpha-hydroxylase gene (CYP7A1) transcription is repressed by bile acids. The goal of this study is to elucidate the mechanism of CYP7A1 transcription by bile acid-activated farnesoid X receptor (FXR) in its native promoter and cellular context and to identify FXR response elements in the gene. In Chinese hamster ovary cells transfected with retinoid X receptor alpha (RXRalpha)/FXR, only chenodeoxycholic acid (CDCA) and deoxycholic acid (DCA) were able to stimulate a heterologous promoter/reporter containing an ecdysone response element. In HepG2 cells, all bile acids (25 microM) were able to repress CYP7A1/luciferase reporter activity, and only CDCA and DCA further repressed reporter activity when cotransfected with RXRalpha/FXR. The concentration of CDCA required to inhibit 50% of reporter activity (IC(50)) was determined to be approximately 25 microM without FXR and 10 microM with FXR. Deletion analysis revealed that the bile acid response element located between nucleotides -148 and -128 was the FXR response element, but RXRalpha/FXR did not bind to this sequence. These results suggest that bile acid-activated FXR exerts its inhibitory effect on CYP7A1 transcription by an indirect mechanism, in contrast to the stimulation and binding of FXR to intestinal bile acid-binding protein gene promoter. Results also reveal that bile acid receptors other than FXR are present in HepG2 cells.
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
*Transcription
2000
Animals
Bile Acids and Salts/*pharmacology
Chiang J Y
Cholesterol 7-alpha-Hydroxylase/*genetics
Cricetinae
Cultured
Cytoplasmic and Nuclear
Department of Integrative Medical Sciences
DNA-Binding Proteins/*physiology
Genetic
Humans
Kimmel R
NEOMED College of Medicine
Promoter Regions
Receptors
Repressor Proteins/*physiology
Response Elements
Retinoic Acid/genetics
Retinoid X Receptors
Stroup D
The Journal of biological chemistry
Transcription Factors/genetics/*physiology
Tumor Cells
Weinberger C
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1016/s0378-1119(00)00518-7" target="_blank" rel="noreferrer noopener">http://doi.org/10.1016/s0378-1119(00)00518-7</a>
Rights
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
Pages
257-265
Issue
1
Volume
262
Search for Full-text
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Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Regulation of cholesterol 7 alpha-hydroxylase gene (CYP7A1) transcription by the liver orphan receptor (LXR alpha)
Publisher
An entity responsible for making the resource available
Gene
Date
A point or period of time associated with an event in the lifecycle of the resource
2001
2001-01
Subject
The topic of the resource
bile acid synthesis; expression; Signaling; dietary-cholesterol; Bile acids; pathway; nuclear receptor; nuclear receptors; promoter; Genetics & Heredity; x-receptor; cytochrome P450; gene regulation; reverse cholesterol transport; hepg2 cells; coup-tfii; ligands
Creator
An entity primarily responsible for making the resource
Chiang J Y L; Kimmel R; Stroup D
Description
An account of the resource
The cholesterol 7 alpha -hydroxylase gene (CYP7A1) plays an important role in regulation of bile acid biosynthesis and cholesterol homeostasis. Oxysterol receptor, LXR, stimulates, whereas the bile acid receptor, FXR, inhibits CYP7A1 transcription. The goal of this study was to investigate the role of LXR alpha on the regulation of rat, human and hamster CYP7A1 transcription in its native promoter and cellular context. Cotransfection with LXR alpha and RXR alpha expression plasmids strongly stimulated rat CYP7A1/luciferase reporter activity in HepG2 cells and oxysterol was not required. However, LXR alpha had much less effect on hamster and no significant effect on human CYP7A1 promoter activity in HepG2 cells. In Chinese hamster ovary cells, cotransfection with LXR alpha stimulated reporter activity by less than 2-fold and addition of 22(R)-hydroxycholesterol caused a small but significant stimulation of rat, human and hamster CYP7A1 promoter activity. At least two direct repeats of AGGTCA-like sequences with 4-base spacing (DR4) and five-base spacing (DR5), in previously identified bile acid response elements of the rat CYP7A1 were able to bind LXR alpha /RXR alpha and confer LXR alpha stimulation. However, LXR alpha did not bind to the corresponding sequences of the human gene and bound weakly to hamster and mouse DR4 sequences. Therefore, rats and mice have the unusual capacity to convert cholesterol to bile acids by LXR alpha -mediated stimulation of CYP7A1 transcription, whereas other species do not respond to cholesterol and develop hypercholesterolemia on a diet high in cholesterol. (C) 2001 Elsevier Science B.V. All rights reserved.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1016/s0378-1119(00)00518-7" target="_blank" rel="noreferrer noopener">10.1016/s0378-1119(00)00518-7</a>
Format
The file format, physical medium, or dimensions of the resource
Journal Article or Conference Abstract Publication
2001
Bile acid synthesis
BILE acids
Chiang J Y L
coup-tfii
cytochrome P450
dietary-cholesterol
expression
gene
Gene Regulation
Genetics & Heredity
hepg2 cells
Journal Article or Conference Abstract Publication
Kimmel R
Ligands
Nuclear Receptor
Nuclear Receptors
pathway
promoter
reverse cholesterol transport
Signaling
Stroup D
x-receptor
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
Pages
257–265
Issue
1
Volume
262
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Regulation of cholesterol 7alpha-hydroxylase gene (CYP7A1) transcription by the liver orphan receptor (LXRalpha).
Publisher
An entity responsible for making the resource available
Gene
Date
A point or period of time associated with an event in the lifecycle of the resource
2001
2001-01
Subject
The topic of the resource
Humans; Animals; Binding Sites; Rats; Species Specificity; Transfection; Gene Expression Regulation/drug effects; Organ Specificity; Transcription Factors/genetics/metabolism; Cricetinae; Response Elements; Luciferases/genetics/metabolism; Retinoid X Receptors; Cholesterol 7-alpha-Hydroxylase/drug effects/*genetics/metabolism; Hydroxycholesterols; Liver/physiology; Lovastatin/pharmacology; Mevalonic Acid/metabolism/pharmacology; Nicotinic Acids/pharmacology; Polyisoprenyl Phosphates/pharmacology; Tetrahydronaphthalenes/pharmacology; Cells; Cultured; Receptors; Transcription; Genetic; Retinoic Acid/genetics/metabolism; Steroid/genetics/*metabolism
Creator
An entity primarily responsible for making the resource
Chiang J Y; Kimmel R; Stroup D
Description
An account of the resource
The cholesterol 7alpha-hydroxylase gene (CYP7A1) plays an important role in regulation of bile acid biosynthesis and cholesterol homeostasis. Oxysterol receptor, LXR, stimulates, whereas the bile acid receptor, FXR, inhibits CYP7A1 transcription. The goal of this study was to investigate the role of LXRalpha on the regulation of rat, human and hamster CYP7A1 transcription in its native promoter and cellular context. Cotransfection with LXRalpha and RXRalpha expression plasmids strongly stimulated rat CYP7A1/luciferase reporter activity in HepG2 cells and oxysterol was not required. However, LXRalpha had much less effect on hamster and no significant effect on human CYP7A1 promoter activity in HepG2 cells. In Chinese hamster ovary cells, cotransfection with LXRalpha stimulated reporter activity by less than 2-fold and addition of 22(R)-hydroxycholesterol caused a small but significant stimulation of rat, human and hamster CYP7A1 promoter activity. At least two direct repeats of AGGTCA-like sequences with 4-base spacing (DR4) and five-base spacing (DR5), in previously identified bile acid response elements of the rat CYP7A1 were able to bind LXRalpha/RXRalpha and confer LXRalpha stimulation. However, LXRalpha did not bind to the corresponding sequences of the human gene and bound weakly to hamster and mouse DR4 sequences. Therefore, rats and mice have the unusual capacity to convert cholesterol to bile acids by LXRalpha-mediated stimulation of CYP7A1 transcription, whereas other species do not respond to cholesterol and develop hypercholesterolemia on a diet high in cholesterol.
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
2001
Animals
Binding Sites
Cells
Chiang J Y
Cholesterol 7-alpha-Hydroxylase/drug effects/*genetics/metabolism
Cricetinae
Cultured
Department of Integrative Medical Sciences
gene
Gene Expression Regulation/drug effects
Genetic
Humans
Hydroxycholesterols
Kimmel R
Liver/physiology
Lovastatin/pharmacology
Luciferases/genetics/metabolism
Mevalonic Acid/metabolism/pharmacology
NEOMED College of Medicine
Nicotinic Acids/pharmacology
Organ Specificity
Polyisoprenyl Phosphates/pharmacology
Rats
Receptors
Response Elements
Retinoic Acid/genetics/metabolism
Retinoid X Receptors
Species Specificity
Steroid/genetics/*metabolism
Stroup D
Tetrahydronaphthalenes/pharmacology
Transcription
Transcription Factors/genetics/metabolism
Transfection