Epithelial Surface Changes And Induction Of Gallstones In The Male Syrian-hamster Gallbladder As A Result Of A 2-month Sex Steroid Treatment
biliary lipids; cholesterol gallstones; contraceptive steroids; crystal-structure; gallbladder; gallstone; hamster; human female; model bile; mucus; ovulatory cycle; Pathology; prairie dog; sex steroid; ultrastructure; x-ray-microanalysis
Gilloteaux J; Kosek E; Kelly T R
Journal of Submicroscopic Cytology and Pathology
1993
1993-10
Journal Article or Conference Abstract Publication
n/a
Sex steroid induction of gallstones in the male Syrian hamster.
Male; Animals; Body Weight; Organ Size; Cricetinae; Drug Combinations; Estradiol/*pharmacology; Mesocricetus; Medroxyprogesterone/*pharmacology; Cholelithiasis/*etiology; Endoplasmic Reticulum/drug effects/ultrastructure; Gallbladder/*drug effects/ultrastructure; Golgi Apparatus/drug effects/ultrastructure; Animal; Disease Models
Light (LM), transmission (TEM) and scanning (SEM) electron microscopic techniques were used to characterize morphologic changes induced in the gallbladder of Syrian hamsters following a one-month estradiol (E) and estradiol + medroxyprogesterone (E+MP) treatment. The TEM results were correlated with the SEM findings. Compared to control (C), E-treated surface epithelial cells contain abundant RER, enlarged Golgi, multivesicular (foamy-heterophagosomes) bodies or lipofuscin inclusions. A 10-day E treatment showed large vesicles develop and, after longer E treatment, they could coalesce and create some of the large multivesicular bodies. Interestingly, E+MP epithelia are characterized by distinct bulging apices where a large number of apical granules accumulate, and contain an anionic mucous core. After a 4-week E+MP treatment, even though all the hamsters were fed a diet with trace cholesterol, significant increase in hamster liver weight, serum level of cholesterol and HDL were measured and, correspondingly, gallstones were found exclusively in E+MP-treated hamsters. Our results showed that not only does the Syrian hamster provide an appropriate model to study experimental lithogenesis without manipulating the diet. In addition, MP appears to induce morphologic changes associated with the formation of gallstones.
Gilloteaux J; Kosek E; Kelly T R
Journal of submicroscopic cytology and pathology
1993
1993-04
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
Epithelial surface changes and induction of gallstones in the male Syrian hamster gallbladder as a result of a two-month sex steroid treatment.
Male; Animals; Body Weight/drug effects; Reference Values; Cricetinae; Mesocricetus; Estradiol/*toxicity; Medroxyprogesterone/*toxicity; Cell Membrane/pathology/ultrastructure; Cholelithiasis/chemically induced/*pathology; Cytoplasmic Granules/ultrastructure; Epithelium/pathology/ultrastructure; Gallbladder/drug effects/*pathology/ultrastructure; Vacuoles/ultrastructure; Microscopy; Electron; Scanning
Transmission (TEM) and scanning (SEM) electron microscopic observations were correlated to characterize morphologic changes induced in the gallbladder of male Syrian hamsters following a two-month estradiol (E) and estradiol + medroxyprogesterone (E + MP) treatment. Compared to control (C), E-treated surface epithelial cells show pleomorphism, cytoplasmic vacuolizations, apical granules, excrescences and decapitations, and small gallstone-like deposits. Following both E + MP treatment, a large accumulation of apical granules containing acidic mucoid products, abundant intraluminal deposits and numerous fields of observation suggest that cell debris and mucous condensation could participate in the formation of the large intraluminal gallstone-like deposits detected as a result of this treatment. In control gallbladders these events were never observed. MP added to E also increases liver and gallbladder weight as well as blood lipid levels. These findings complement and confirm other previous data obtained following short steroid treatment in male, ovariectomized and intact female hamsters. In addition, these results support our hypothesis that gallstone nucleation and growth originate from multiple factors, hormonal disturbance, modulation of liver lipid metabolism, production of cell debris and mucus, can be responsible for the initial gallstone nucleation.
Gilloteaux J; Kosek E; Kelly T R
Journal of submicroscopic cytology and pathology
1993
1993-10
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).