HAS and LAS rats respond differentially to behavioral effects of ethanol, pentobarbital, chlorpromazine and chlordiazepoxide.
Male; Animals; Rats; Ethanol/*pharmacology; Behavior; Discrimination (Psychology)/drug effects; Motor Activity/drug effects; Sleep/drug effects; *Hypnotics and Sedatives; Chlordiazepoxide/*pharmacology; Chlorpromazine/*pharmacology; Pentobarbital/*pharmacology; Animal/*drug effects
The drug discrimination paradigm (DD) was used to evaluate differences in performance of rats selectively bred for differential sensitivity to the hypnotic effects of ethanol. Tenth generation high-alcohol sensitive (HAS) and low-alcohol sensitive (LAS) rats were trained to discriminate between ethanol (1.0 g/kg, IP) and saline vehicle on a VR-5 schedule of reinforcement. The HAS strain was more sensitive to the discriminative effects of ethanol than the LAS strain, but the magnitude of difference was much smaller than the differential sleep-time differences. The biphasic action of ethanol was differentially seen when the LAS animals exhibited increased activity during both DD and spontaneous motor activity measures and the HAS exhibited decreased activity during DD only. Pentobarbital and chlordiazepoxide but not chlorpromazine elicited the ethanol discriminative choice in both HAS and LAS strains. Response rates during DD indicated a dissociation of rate depressant effects and discriminative performance following ethanol. These findings are discussed in relationship to some current and future uses of selectively bred animal strains and DD for studying the effects of alcohol.
Krimmer E C
Pharmacology, biochemistry, and behavior
1991
1991-05
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
<a href="http://doi.org/10.1016/0091-3057(91)90389-j" target="_blank" rel="noreferrer noopener">10.1016/0091-3057(91)90389-j</a>
Ethanol interoceptive cue and sleep-time duration in HAS and LAS selectively bred rats.
Male; Time Factors; Animals; Phenotype; Rats; Species Specificity; Ethanol/*pharmacology; *Cues; *Discrimination Learning; *Hypnotics and Sedatives; Breeding; Sleep/*drug effects
The drug discrimination paradigm was used to evaluate the effects of selective breeding for differential sensitivity to the hypnotic effects of ethanol. Tenth generation high alcohol sensitive (HAS) and low alcohol sensitive (LAS) rats were trained to discriminate between ethanol (0.6 g/kg, IP) and saline vehicle on a
Krimmer E C
Pharmacology, biochemistry, and behavior
1990
1990-06
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
<a href="http://doi.org/10.1016/0091-3057(90)90400-c" target="_blank" rel="noreferrer noopener">10.1016/0091-3057(90)90400-c</a>
HAD and LAD rats respond differently to stimulating effect but not discriminative effects of ethanol.
*Discrimination Learning; Alcohol Drinking/*physiopathology; Animals; Ethanol/administration & dosage/*pharmacology; Male; Rats
The drug discrimination paradigm (DD) was used to evaluate behavioral differences of rats selectively bred for differential ethanol drinking preferences. Seventh-generation high alcohol-drinking (HAD) and low alcohol-drinking (LAD) rats were trained to discriminate between ethanol (0.5 g/kg, IP) and saline vehicle, following a 2-min presession interval (PI), using an FR-10 schedule of reinforcement. The HAD line was more responsive than the LAD line to the stimulating effect of ethanol as measured by total response rates. ED50 values of 0.239 and 0.244 g/kg for the HAD and LAD lines, respectively, do not reflect any difference in the discriminative effects of ethanol. Response rates during DD indicated a dissociation of rate-increasing effects and discriminative performance following ethanol. In addition to differential drinking preference, these data suggest that selective breeding for the HAD and LAD animals also involves the stimulant action of ethanol but not on the discriminative effects.
Krimmer E C; Schechter M D
Alcohol (Fayetteville, N.Y.)
1992
1992-02
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
<a href="http://doi.org/10.1016/0741-8329(92)90012-y" target="_blank" rel="noreferrer noopener">10.1016/0741-8329(92)90012-y</a>
Biphasic effects of ethanol tested with drug discrimination in HAD and LAD rats.
Alcohol Drinking/genetics/*psychology; Animals; Discrimination (Psychology)/*drug effects; Discrimination Learning/drug effects; Dose-Response Relationship; Drug; Ethanol/*pharmacology; Inbred Strains; Male; Pentobarbital/pharmacology; Rats; Serotonin Antagonists/pharmacology; Sleep/drug effects; Time Factors; Tropanes/pharmacology
Seventh-generation selectively bred high-alcohol-drinking (HAD) and low-alcohol-drinking (LAD) rats were trained to make differential responses for ethanol (0.75 g/kg, IP) and saline vehicle, following postadministration intervals (PI) of 2 min (HAD-2 and LAD-2 animals) and 30 min (HAD-30 and LAD-30 animals). ED50 values of 0.395 and 0.352 g/kg, respectively, for HAD-2 and LAD-2 animals and 0.269 and 0.314 g/kg, respectively, for HAD-30 and LAD-30 animals reflect the absence of any phenotypic difference for the discriminative stimulus effects of ethanol. HAD-2 animals were more responsive than LAD-2 animals to the stimulating effects of ethanol as measured by total response rates during training sessions. The differential ethanol response generalized to pentobarbital in all four groups but not to morphine, an alternative CNS depressant. The specific antagonist of 5-hydroxytryptamine3 receptors,
Krimmer E C
Pharmacology, biochemistry, and behavior
1992
1992-12
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
<a href="http://doi.org/10.1016/0091-3057(92)90508-d" target="_blank" rel="noreferrer noopener">10.1016/0091-3057(92)90508-d</a>
Differences in response to the aversive properties and activity effects of low dose ethanol in LAS and HAS selectively bred rats.
Animals; Avoidance Learning/*drug effects; Conditioning; Dose-Response Relationship; Drug; Ethanol/*pharmacology; Motor Activity/drug effects; Operant/drug effects; Rats; Sleep/drug effects; Species Specificity
Rats selectively bred for high alcohol sleep times (HAS) and those that are less affected (LAS) by hypnotic doses (3.0-3.6 g/kg) of ethanol were tested for differential responses to the aversive effects of 1.0 g/kg ethanol in a conditioned place preference task. Likewise, the effects of 0.3-1.0 g/kg ethanol on spontaneous locomotor activity over a 30-min period, as well as the loss of righting reflex with a higher ethanol dose (3.0 g/kg), were determined in these animals. The LAS rats reacted more aversively to 1.0 g/kg during conditioned place aversion testing than the HAS animals and also had a shorter mean sleeping time following 3.0 g/kg ethanol. Furthermore, dose-related depression of spontaneous motor activity was seen in the HAS animals and not in the LAS animals over a 30-min period using doses of 0.3, 0.6, or 1.0 g/kg (10% w/v) ethanol. Taken together, the results indicate that the intoxicating sequelae of high ethanol doses, such as ataxia and sedation, may not be correlated with the aversive effects of low ethanol doses.
Schechter M D; Krimmer E C
Psychopharmacology
1992
1905-6
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
<a href="http://doi.org/10.1007/bf02245271" target="_blank" rel="noreferrer noopener">10.1007/bf02245271</a>