1
40
1
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1042/BJ20141494" target="_blank" rel="noreferrer noopener">http://doi.org/10.1042/BJ20141494</a>
Pages
181–193
Issue
2
Volume
470
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Orphan nuclear receptor oestrogen-related receptor gamma (ERRgamma) plays a key role in hepatic cannabinoid receptor type 1-mediated induction of CYP7A1 gene expression.
Publisher
An entity responsible for making the resource available
The Biochemical journal
Date
A point or period of time associated with an event in the lifecycle of the resource
2015
2015-09
Subject
The topic of the resource
Animals; bile acid; Bile Acids and Salts/metabolism; Cannabinoid; cannabinoid receptors; CB1/agonists/genetics/*metabolism; Cells; Cholesterol 7-alpha-Hydroxylase/*biosynthesis/genetics; cholesterol 7alpha-hydroxylase (CYP7A1); Cultured; Cytoplasmic and Nuclear/metabolism; Drug Inverse Agonism; Estrogen/genetics/*metabolism; Ethanol/pharmacology; Gene Expression; Genetic; Glycerides/pharmacology; GSK5182; HEK293 Cells; Hepatocytes/metabolism; Humans; Inbred C57BL; Knockout; Liver/*metabolism; Mice; oestrogen-related receptor gamma (ERRgamma); orphan nuclear receptor; Promoter Regions; Rats; Receptor; Receptors; small heterodimer partner (SHP); Sprague-Dawley; Transcription
Creator
An entity primarily responsible for making the resource
Zhang Yaochen; Kim Don-Kyu; Lee Ji-Min; Park Seung Bum; Jeong Won-Il; Kim Seong Heon; Lee In-Kyu; Lee Chul-Ho; Chiang John Y L; Choi Hueng-Sik
Description
An account of the resource
Bile acids are primarily synthesized from cholesterol in the liver and have important roles in dietary lipid absorption and cholesterol homoeostasis. Detailed roles of the orphan nuclear receptors regulating cholesterol 7alpha-hydroxylase (CYP7A1), the rate-limiting enzyme in bile acid synthesis, have not yet been fully elucidated. In the present study, we report that oestrogen-related receptor gamma (ERRgamma) is a novel transcriptional regulator of CYP7A1 expression. Activation of cannabinoid receptor type 1 (CB1 receptor) signalling induced ERRgamma-mediated transcription of the CYP7A1 gene. Overexpression of ERRgamma increased CYP7A1 expression in vitro and in vivo, whereas knockdown of ERRgamma attenuated CYP7A1 expression. Deletion analysis of the CYP7A1 gene promoter and a ChIP assay revealed an ERRgamma-binding site on the CYP7A1 gene promoter. Small heterodimer partner (SHP) inhibited the transcriptional activity of ERRgamma and thus regulated CYP7A1 expression. Overexpression of ERRgamma led to increased bile acid levels, whereas an inverse agonist of ERRgamma, GSK5182, reduced CYP7A1 expression and bile acid synthesis. Finally, GSK5182 significantly reduced hepatic CB1 receptor-mediated induction of CYP7A1 expression and bile acid synthesis in alcohol-treated mice. These results provide the molecular mechanism linking ERRgamma and bile acid metabolism.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1042/BJ20141494" target="_blank" rel="noreferrer noopener">10.1042/BJ20141494</a>
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
2015
Animals
bile acid
Bile Acids and Salts/metabolism
Cannabinoid
cannabinoid receptors
CB1/agonists/genetics/*metabolism
Cells
Chiang John Y L
Choi Hueng-Sik
Cholesterol 7-alpha-Hydroxylase/*biosynthesis/genetics
cholesterol 7alpha-hydroxylase (CYP7A1)
Cultured
Cytoplasmic and Nuclear/metabolism
Department of Integrative Medical Sciences
Drug Inverse Agonism
Estrogen/genetics/*metabolism
Ethanol/pharmacology
Gene Expression
Genetic
Glycerides/pharmacology
GSK5182
HEK293 Cells
Hepatocytes/metabolism
Humans
Inbred C57BL
Jeong Won-Il
Kim Don-Kyu
Kim Seong Heon
Knockout
Lee Chul-Ho
Lee In-Kyu
Lee Ji-Min
Liver/*metabolism
Mice
NEOMED College of Medicine
oestrogen-related receptor gamma (ERRgamma)
orphan nuclear receptor
Park Seung Bum
Promoter Regions
Rats
Receptor
Receptors
small heterodimer partner (SHP)
Sprague-Dawley
The Biochemical journal
Transcription
Zhang Yaochen