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Text
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URL Address
<a href="http://doi.org/10.1038/onc.2015.83" target="_blank" rel="noreferrer noopener">http://doi.org/10.1038/onc.2015.83</a>
Pages
314–322
Issue
3
Volume
35
Dublin Core
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Title
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Activation of mechanosensitive ion channel TRPV4 normalizes tumor vasculature and improves cancer therapy.
Publisher
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Oncogene
Date
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2016
2016-01
Subject
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Animals; Calcium Signaling/genetics; Carcinoma; Cell Line; Cell Proliferation/drug effects; Cisplatin/administration & dosage; Endothelium; Gene Expression Regulation; Humans; Leucine/administration & dosage/analogs & derivatives; Lewis Lung/drug therapy/*genetics/pathology; Mice; Neoplastic/drug effects; Neovascularization; Pathologic/drug therapy/*genetics/pathology; Sulfonamides/administration & dosage; TRPV Cation Channels/agonists/biosynthesis/*genetics; Tumor; Vascular Endothelial Growth Factor A/genetics; Vascular/drug effects/*pathology
Creator
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Adapala R K; Thoppil R J; Ghosh K; Cappelli H C; Dudley A C; Paruchuri S; Keshamouni V; Klagsbrun M; Meszaros J G; Chilian W M; Ingber D E; Thodeti C K
Description
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Tumor vessels are characterized by abnormal morphology and hyperpermeability that together cause inefficient delivery of chemotherapeutic agents. Although vascular endothelial growth factor has been established as a critical regulator of tumor angiogenesis, the role of mechanical signaling in the regulation of tumor vasculature or tumor endothelial cell (TEC) function is not known. Here we show that the mechanosensitive ion channel transient receptor potential vanilloid 4 (TRPV4) regulates tumor angiogenesis and tumor vessel maturation via modulation of TEC mechanosensitivity. We found that TECs exhibit reduced TRPV4 expression and function, which is correlated with aberrant mechanosensitivity towards extracellular matrix stiffness, increased migration and abnormal angiogenesis by TEC. Further, syngeneic tumor experiments revealed that the absence of TRPV4 induced increased vascular density, vessel diameter and reduced pericyte coverage resulting in enhanced tumor growth in TRPV4 knockout mice. Importantly, overexpression or pharmacological activation of TRPV4 restored aberrant TEC mechanosensitivity, migration and normalized abnormal angiogenesis in vitro by modulating Rho activity. Finally, a small molecule activator of TRPV4, GSK1016790A, in combination with anticancer drug cisplatin, significantly reduced tumor growth in wild-type mice by inducing vessel maturation. Our findings demonstrate TRPV4 channels to be critical regulators of tumor angiogenesis and represent a novel target for anti-angiogenic and vascular normalization therapies.
Identifier
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<a href="http://doi.org/10.1038/onc.2015.83" target="_blank" rel="noreferrer noopener">10.1038/onc.2015.83</a>
Rights
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Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
2016
Adapala R K
Animals
Calcium Signaling/genetics
Cappelli H C
Carcinoma
Cell Line
Cell Proliferation/drug effects
Chilian W M
Cisplatin/administration & dosage
Department of Integrative Medical Sciences
Dudley A C
Endothelium
Gene Expression Regulation
Ghosh K
Humans
Ingber D E
Keshamouni V
Klagsbrun M
Leucine/administration & dosage/analogs & derivatives
Lewis Lung/drug therapy/*genetics/pathology
Meszaros J G
Mice
NEOMED College of Medicine
Neoplastic/drug effects
Neovascularization
Oncogene
Paruchuri S
Pathologic/drug therapy/*genetics/pathology
Sulfonamides/administration & dosage
Thodeti C K
Thoppil R J
TRPV Cation Channels/agonists/biosynthesis/*genetics
Tumor
Vascular Endothelial Growth Factor A/genetics
Vascular/drug effects/*pathology