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<a href="http://doi.org/10.1016/j.expneurol.2017.06.020" target="_blank" rel="noreferrer noopener">http://doi.org/10.1016/j.expneurol.2017.06.020</a>
Pages
1–15
Volume
296
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Title
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Injury-induced gp130 cytokine signaling in peripheral ganglia is reduced in diabetes mellitus.
Publisher
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Experimental neurology
Date
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2017
2017-10
Subject
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*Axon regeneration; *Axotomy; *Diabetes; *Neuropathy; *Peripheral nervous system; Animal; Animals; Antibiotics; Antineoplastic/toxicity; Blood Glucose/drug effects; Body Weight/drug effects; Cytokine Receptor gp130/genetics/*metabolism; Cytokines/metabolism; Diabetes Mellitus; Disease Models; Experimental/chemically induced/complications/*pathology; Fasting/blood; Gene Expression Regulation/*physiology; Hyperalgesia/etiology; Hyperglycemia/etiology; Inbred C57BL; Male; Mice; Nerve Degeneration/*etiology/pathology; Nerve Tissue Proteins/metabolism; Pain Measurement; Signal Transduction/drug effects/*physiology; Streptozocin/toxicity; Superior Cervical Ganglion/drug effects/*metabolism; Sweating/drug effects
Creator
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Niemi Jon P; Filous Angela R; DeFrancesco Alicia; Lindborg Jane A; Malhotra Nisha A; Wilson Gina N; Zhou Bowen; Crish Samuel D; Zigmond Richard E
Description
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Neuropathy is a major diabetic complication. While the mechanism of this neuropathy is not well understood, it is believed to result in part from deficient nerve regeneration. Work from our laboratory established that gp130 family of cytokines are induced in animals after axonal injury and are involved in the induction of regeneration-associated genes (RAGs) and in the conditioning lesion response. Here, we examine whether a reduction of cytokine signaling occurs in diabetes. Streptozotocin (STZ) was used to destroy pancreatic beta cells, leading to chronic hyperglycemia. Mice were injected with either low doses of STZ (5x60mg/kg) or a single high dose (1x200mg/kg) and examined after three or one month, respectively. Both low and high dose STZ treatment resulted in sustained hyperglycemia and functional deficits associated with the presence of both sensory and autonomic neuropathy. Diabetic mice displayed significantly reduced intraepidermal nerve fiber density and sudomotor function. Furthermore, low and high dose diabetic mice showed significantly reduced tactile touch sensation measured with Von Frey monofilaments. To look at the regenerative and injury-induced responses in diabetic mice, neurons in both superior cervical ganglia (SCG) and the 4th and 5th lumbar dorsal root ganglia (DRG) were unilaterally axotomized. Both high and low dose diabetic mice displayed significantly less axonal regeneration in the sciatic nerve, when measured in vivo, 48h after crush injury. Significantly reduced induction of two gp130 cytokines, leukemia inhibitory factor and interleukin-6, occurred in diabetic animals in SCG 6h after injury compared to controls. Injury-induced expression of interleukin-6 was also found to be significantly reduced in the DRG at 6h after injury in low and high dose diabetic mice. These effects were accompanied by reduced phosphorylation of signal transducer and activator of transcription 3 (STAT3), a downstream effector of the gp130 signaling pathway. We also found decreased induction of several gp130-dependent RAGs, including galanin and vasoactive intestinal peptide. Together, these data suggest a novel mechanism for the decreased response of diabetic sympathetic and sensory neurons to injury.
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<a href="http://doi.org/10.1016/j.expneurol.2017.06.020" target="_blank" rel="noreferrer noopener">10.1016/j.expneurol.2017.06.020</a>
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Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
*Axon regeneration
*Axotomy
*Diabetes
*Neuropathy
*Peripheral nervous system
2017
Animal
Animals
Antibiotics
Antineoplastic/toxicity
Blood Glucose/drug effects
Body Weight/drug effects
Crish Samuel D
Cytokine Receptor gp130/genetics/*metabolism
Cytokines/metabolism
DeFrancesco Alicia
Department of Pharmaceutical Sciences
Diabetes Mellitus
Disease Models
Experimental neurology
Experimental/chemically induced/complications/*pathology
Fasting/blood
Filous Angela R
Gene Expression Regulation/*physiology
Hyperalgesia/etiology
Hyperglycemia/etiology
Inbred C57BL
Lindborg Jane A
Male
Malhotra Nisha A
Mice
NEOMED College of Pharmacy
Nerve Degeneration/*etiology/pathology
Nerve Tissue Proteins/metabolism
Niemi Jon P
Pain Measurement
Signal Transduction/drug effects/*physiology
Streptozocin/toxicity
Superior Cervical Ganglion/drug effects/*metabolism
Sweating/drug effects
Wilson Gina N
Zhou Bowen
Zigmond Richard E