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URL Address
<a href="http://doi.org/10.1073/pnas.170295297" target="_blank" rel="noreferrer noopener">http://doi.org/10.1073/pnas.170295297</a>
Pages
9967–9971
Issue
18
Volume
97
Dublin Core
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Title
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Using O2 to probe membrane immersion depth by 19F NMR.
Publisher
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Proceedings of the National Academy of Sciences of the United States of America
Date
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2000
2000-08
Subject
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*Hydrocarbons; *Oxygen; Biological; Cyclic N-Oxides; Dimyristoylphosphatidylcholine/*chemistry; Fluorinated; Fluorine; Lipid Bilayers/*chemistry; Magnetic Resonance Spectroscopy/*methods; Maltose/*analogs & derivatives; Models; Phospholipid Ethers/*chemistry; Pressure; Spin Labels
Creator
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Prosser R S; Luchette P A; Westerman P W
Description
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A fluorinated detergent, CF(3)(CF(2))(5)C(2)H(4)-O-maltose, was reconstituted into a lipid bilayer model membrane system to demonstrate the feasibility of determining solvent accessibility and membrane immersion depth of each fluorinated group by (19)F NMR. Apolar oxygen, which is known to partition with an increasing concentration gradient toward the hydrophobic membrane interior, exhibits a range of paramagnetic relaxation effects on (19)F nuclei, depending on its depth in the membrane. This effect, which is predominately associated with spin-lattice relaxation rates (R(1)) and chemical shifts, can be amplified greatly with minimal line broadening by increasing the partial pressure of O(2) at least 100-fold (i.e., P(O(2)) greater than 20 bar). The differences of longitudinal relaxation rates at 20 bar of oxygen pressure to those under ambient pressure (R(1)(20bar) - R(1)(0)) are largest for those fluorine groups expected to be most deeply buried in the membrane bilayer. This result contrasts with the reverse trend, which is observed on addition of a membrane surface-associated paramagnetic species, 4-(N,N-dimethyl-N-hexadecyl) ammonium-2,2,6,6-tetramethylpiperidine-1-oxyl iodide (CAT-16) at ambient pressures. Thus, differential relaxation rates may be observed in (19)F-labeled membrane-associated molecules resulting from the addition of apolar oxygen under high pressure. The results demonstrate that the degree of solvent accessibility and membrane immersion depth of specific fluorinated species in membrane-associated macromolecules can be probed by (19)F NMR.
Identifier
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<a href="http://doi.org/10.1073/pnas.170295297" target="_blank" rel="noreferrer noopener">10.1073/pnas.170295297</a>
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Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
*Hydrocarbons
*Oxygen
2000
Biological
Cyclic N-Oxides
Dimyristoylphosphatidylcholine/*chemistry
Fluorinated
Fluorine
Lipid Bilayers/*chemistry
Luchette P A
Magnetic Resonance Spectroscopy/*methods
Maltose/*analogs & derivatives
Models
Phospholipid Ethers/*chemistry
Pressure
Proceedings of the National Academy of Sciences of the United States of America
Prosser R S
Spin Labels
Westerman P W