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40
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Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
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<a href="http://doi.org/10.3390/ijms21165675" target="_blank" rel="noreferrer noopener">http://doi.org/10.3390/ijms21165675</a>
Issue
16
Volume
21
ISSN
1422-0067 1422-0067
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August 2020 List
NEOMED College
NEOMED College of Medicine
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Department of Anatomy & Neurobiology
NEOMED Postdoc Publications
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Title
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Small non-coding RNAome of ageing chondrocytes.
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International Journal of Molecular Sciences
Date
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2020
2020-08-07
Subject
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chondrocyte; ageing; equine; small non-coding RNA
Creator
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Balaskas P; Green JA; Haqqi TM; Dyer P; Kharaz YA; Fang Y; Liu X; Welting TJM; Peffers MJ
Description
An account of the resource
Ageing is a leading risk factor predisposing cartilage to osteoarthritis. However, little research has been conducted on the effect of ageing on the expression of small non-coding RNAs (sncRNAs). RNA from young and old chondrocytes from macroscopically normal equine metacarpophalangeal joints was extracted and subjected to small RNA sequencing (RNA-seq). Differential expression analysis was performed in R using package DESeq2. For transfer RNA (tRNA) fragment analysis, tRNA reads were aligned to horse tRNA sequences using Bowtie2 version 2.2.5. Selected microRNA (miRNAs or miRs) and small nucleolar RNA (snoRNA) findings were validated using real-time quantitative Polymerase Chain Reaction (qRT-PCR) in an extended cohort of equine chondrocytes. tRNA fragments were further investigated in low- and high-grade OA human cartilage tissue. In total, 83 sncRNAs were differentially expressed between young and old equine chondrocytes, including miRNAs, snoRNAs, small nuclear RNAs (snRNAs), and tRNAs. qRT-PCR analysis confirmed findings. tRNA fragment analysis revealed that tRNA halves (tiRNAs), tiRNA-5035-GluCTC and tiRNA-5031-GluCTC-1 were reduced in both high grade OA human cartilage and old equine chondrocytes. For the first time, we have measured the effect of ageing on the expression of sncRNAs in equine chondrocytes. Changes were detected in a number of different sncRNA species. This study supports a role for sncRNAs in ageing cartilage and their potential involvement in age-related cartilage diseases.
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<a href="http://doi.org/10.3390/ijms21165675" target="_blank" rel="noreferrer noopener">10.3390/ijms21165675</a>
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journalArticle
2020
ageing
August 2020 List
Balaskas P
chondrocyte
Department of Anatomy & Neurobiology
Dyer P
equine
Fang Y
Green JA
Haqqi TM
International journal of molecular sciences
journalArticle
Kharaz YA
Liu X
NEOMED College of Medicine
NEOMED College of Medicine Postdoc
NEOMED Postdoc Publications
Peffers MJ
small non-coding RNA
Welting TJM
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
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n/a
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Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
Pages
E145-E145
Volume
51
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Title
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Introduction: Overview of the Feeding Experiments End-User Database (FEED)
Publisher
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Integrative and Comparative Biology
Date
A point or period of time associated with an event in the lifecycle of the resource
2011
2011-03
Subject
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Zoology
Creator
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Wall C E; Vinyard C J; Williams S H; German R Z; Gapeyev V; Liu X
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n/a
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Journal Article
2011
Gapeyev V
German R Z
Integrative and comparative biology
Journal Article
Liu X
Vinyard C J
Wall C E
Williams S H
Zoology
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1016/j.jsxm.2019.01.194" target="_blank" rel="noreferrer noopener">http://doi.org/10.1016/j.jsxm.2019.01.194</a>
Pages
S92–S92
Issue
4
Volume
16
ISSN
1743-6095
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<p>Users with a NEOMED Library login can search for full-text journal articles at the following url: <a href="https://libraryguides.neomed.edu/home">https://libraryguides.neomed.edu/home</a></p>
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Title
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IMPACT OF RHOGDI GENE TRANSFECTION OF BLADDER SMOOTH MUSCLE CONTRACTILITY IN A VALIDATED EX-VIVO MURINE MODEL
Publisher
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Journal of Sexual Medicine
Date
A point or period of time associated with an event in the lifecycle of the resource
2019
2019-04
Subject
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Urology & Nephrology
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Joice G; Bell J M; La Favor J; Yoshida T; Torga G; Harris K; Liu X; Kiedrowski M; Penn M; Bivalacqua T
Identifier
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<a href="http://doi.org/10.1016/j.jsxm.2019.01.194" target="_blank" rel="noreferrer noopener">10.1016/j.jsxm.2019.01.194</a>
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Description
An account of the resource
19th Annual Fall Scientific Meeting of SMSNA
Introduction Plasmid-based gene therapy is an intriguing option for treating malignant and bladder pathologies. The RhoA pathway is involved in bladder smooth muscle regulation, cancer invasion and metastasis. Rho GDP-dissociation inhibitor (RhoGDI) is an inhibitor of the RhoA pathway. We validated ex-vivo bladder gene transfer to facilitate assessment of gene targets for treating bladder pathology. Methods Basic Local Alignment Search Tool was used to identify human RhoGDI coding sequences and to compare between rats and humans, which were cloned into a eCMV-based expression vector. NBTII rat bladder cancer cell lines were transfected using FuGENE (Promega, USA) and human protein expression and interaction with endogenous RhoA were tested using flow cytometry, immunofluorescence, and RNA expression analysis. Bladders were harvested from female Lewis Rats (∼250g) and sectioned and cultured for 72-hours following transfection with RhoGDI and FuGENE. Transfected bladder tissues were analyzed as described above. Non-transfected cultured bladder segments were analyzed using myography for viability and intact smooth muscle physiology in response to 120 mM KCl and 30uM carbachol. Results Human and rodent RhoGDI protein homology is 96%. Human RhoGDI was successfully detected exclusively in transfected NBTII cells with a top efficiency of 26%. qPCR analysis demonstrated rodent RhoA and RhoGDI levels were not impacted but ROCK1 and ROCK2 mRNA were significantly decreased by 23.6% (p=0.034) and 40.0% (p=0.015), respectively following human RhoGDI transfection. Human RhoGDI was detected in transfected ex-vivo cultured bladder segments in both FuGENE and microinjection experiments. Similar to NBTII cells, qPCR analysis demonstrated rodent RhoA and RhoGDI levels were not impacted but ROCK1 and ROCK2 mRNA were significantly decreased by 15.0% (p=0.035) and 22.4% (p=0.010) after FuGENE transfection and 20.5% (p=0.024) and 21.4% (p=0.015) after microinjections. Ex-vivo cultured bladder strips successfully contracted to KCl (mean 0.88+/-0.48 mN/mg tissue) and carbachol (1.82+/-0.97 mN/mg tissue) stimulation. After microinjection, RhoGDI caused a significant reduction in KCl mediated constriction although this was not observed in FuGENE experiments. Conclusion Ex-vivo bladder culture, transfection, and physiological assessment are feasible and may provide a high-throughput method to test novel gene transfer technologies before in-vivo testing. RhoGDI plasmid microinjection transfection appears to decrease contractility of ex-vivo bladder smooth muscle.
2019
Bell J M
Bivalacqua T
Department of Integrative Medical Sciences
Harris K
Joice G
Journal of Sexual Medicine
June 2019 Update
Kiedrowski M
La Favor J
Liu X
NEOMED College of Medicine
Penn M
Torga G
Urology & Nephrology
Yoshida T
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="https://doi.org/10.1016/j.jsxm.2019.01.194">https://doi.org/10.1016/j.jsxm.2019.01.194</a>
Rights
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
Pages
S92-S92
Issue
4
Volume
16
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
185 Impact of RhoGDI Gene Transfection of Bladder Smooth Muscle Contractility in a Validated Ex-vivo Murine Model
Publisher
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The Journal of Sexual Medicine
Date
A point or period of time associated with an event in the lifecycle of the resource
2019
2019-04
Creator
An entity primarily responsible for making the resource
Joice G; Bell J M; La Favor J; Yoshida T; Torga G; Harris K; Liu X; Kiedrowski M; Penn M; Bivalacqua T
Identifier
An unambiguous reference to the resource within a given context
<a href="https://doi.org/10.1016/j.jsxm.2019.01.194">https://doi.org/10.1016/j.jsxm.2019.01.194</a>
2019
Bell J M
Bivalacqua T
Department of Internal Medicine
Harris K
Joice G
June 2019 Update
Kiedrowski M
La Favor J
Liu X
NEOMED College of Medicine
Penn M
The Journal of Sexual Medicine
Torga G
Yoshida T