1 40 1 Text A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text. URL Address <a href="http://doi.org/10.1161/CIRCRESAHA.120.317715" target="_blank" rel="noreferrer noopener">http://doi.org/10.1161/CIRCRESAHA.120.317715</a> Rights Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s). ISSN 1524-4571 0009-7330 Search for Full-text Locate full-text within NEOMED Library's e-journal collections <a href="http://neomed.idm.oclc.org/login?url=http://doi.org/10.1161/CIRCRESAHA.120.317715" target="_blank" rel="noreferrer noopener">NEOMED Full-text Holding (if available) - Proxy DOI: 10.1161/CIRCRESAHA.120.317715</a> <p>Users with a NEOMED Library login can search for full-text journal articles at the following url: <a href="https://libraryguides.neomed.edu/home">https://libraryguides.neomed.edu/home</a></p> Update Year & Number February 2021 List NEOMED College NEOMED College of Medicine NEOMED Department Department of Integrative Medical Sciences Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Myocardial Blood Flow Control by Oxygen Sensing Vascular Kvβ Proteins. Date A point or period of time associated with an event in the lifecycle of the resource 2021 2021-01-27 Subject The topic of the resource coronary arteris; microcirculation; myogenic tone; nicotinamide adenine dinucleotide; voltage-gated potassium channels Creator An entity primarily responsible for making the resource Ohanyan V;Raph SM;Dwenger MM;Hu X;Pucci T;Mack GD;Moore IJB;Chilian WM;Bhatnagar A;Nystoriak MA Description An account of the resource Rationale: Voltage-gated potassium (Kv) channels in vascular smooth muscle are essential for coupling myocardial blood flow (MBF) with the metabolic demand of the heart. These channels consist of a transmembrane pore domain that associates with auxiliary Kvβ1 and Kvβ2 proteins, which differentially regulate Kv function in excitable cells. Nonetheless, the physiological role of Kvβ proteins in regulating vascular tone and metabolic hyperemia in the heart remains unknown. Objective: To test the hypothesis that Kvβ proteins confer oxygen sensitivity to vascular tone and are required for regulating blood flow in the heart. Methods and Results: Mice lacking Kvβ2 subunits exhibited suppressed MBF, impaired cardiac contractile performance, and failed to maintain elevated arterial blood pressure in response to catecholamine-induced stress. In contrast, ablation of Kvβ1.1 reduced cardiac workload, modestly elevated MBF, and preserved cardiac function during stress compared with wild type mice. Coronary arteries isolated from Kvβ2-/-, but not Kvβ1.1-/-, mice, had severely blunted vasodilation to hypoxia when compared with arteries from wild type mice. Moreover, vasodilation of small diameter coronary and mesenteric arteries due to L-lactate, a biochemical marker of reduced tissue oxygenation and anaerobic metabolism, was significantly attenuated in vessels isolated from Kvβ2-/- mice. Inducible enhancement of the Kvβ1:Kvβ2 ratio in Kv1 channels of arterial smooth muscle abolished L-lactate-induced vasodilation and suppressed the relationship between MBF and cardiac workload.Conclusions: The Kvβ proteins differentially regulate vascular tone and myocardial blood flow, whereby Kvβ2 promotes and Kvβ1.1 inhibits oxygen-dependent vasodilation and augments blood flow upon heightened metabolic demand. Identifier An unambiguous reference to the resource within a given context <a href="http://doi.org/10.1161/CIRCRESAHA.120.317715" target="_blank" rel="noreferrer noopener">10.1161/CIRCRESAHA.120.317715</a> Format The file format, physical medium, or dimensions of the resource journalArticle Publisher An entity responsible for making the resource available Circulation Research 2021 Bhatnagar A Chilian WM Circulation research coronary arteris Department of Integrative Medical Sciences Dwenger MM February 2021 List Hu X journalArticle Mack GD Microcirculation Moore IJB myogenic tone NEOMED College of Medicine nicotinamide adenine dinucleotide Nystoriak MA Ohanyan V Pucci T Raph SM voltage-gated potassium channels