1
40
1
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1006/cbir.2001.0818" target="_blank" rel="noreferrer noopener">http://doi.org/10.1006/cbir.2001.0818</a>
Pages
131–144
Issue
2
Volume
26
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Pertubation of beta1 integrin function using anti-sense or function-blocking antibodies on corneal cells grown on fibronectin and tenascin.
Publisher
An entity responsible for making the resource available
Cell biology international
Date
A point or period of time associated with an event in the lifecycle of the resource
2002
1905-6
Subject
The topic of the resource
Animals; Antisense/*pharmacology; Cattle; Cell Adhesion; Cell Culture Techniques/*methods; Cells; Chick Embryo; Chickens; Complementary/metabolism; Cornea/*cytology; Cultured; DNA; Dose-Response Relationship; Drug; Fibronectins/*metabolism; Fluorescence; Immunohistochemistry; Integrin beta1/*metabolism/*physiology; Integrins/metabolism; Microscopy; Oligonucleotides; Protein Binding; Retroviridae/genetics; Tenascin/*metabolism
Creator
An entity primarily responsible for making the resource
Doane Kathleen J; Bhattacharya Raka; Marchant Jeff
Description
An account of the resource
During corneal development, neural crest derivatives from the periocular mesenchyme migrate into the cornea and differentiate into corneal fibroblasts. During this time, these cells interact with a variety of extracellular matrices for proper orientation and development. In the present studies, we have examined the interaction of beta(1) integrins on periocular mesenchyme cells (POM) and corneal fibroblasts (CF) with fibronectin and tenascin by perturbing the function of this integrin. POM and CF attached and spread to a much greater extent on fibronectin than on tenascin. An antibody against beta(1) integrin, CSAT, decreased spreading and attachment, and resulted in a lack of immuno-detectable beta(1) integrin in focal adhesions on fibronectin; few beta(1) positive focal adhesions were observed in cells grown on tenascin. An anti-sense retroviral construct decreased endogenous levels of beta(1) integrin protein, and caused decreased attachment and spreading as well as sparse, disorganized focal adhesions. These data indicate that in vitro, both POM and CF have beta(1) integrins that interact with fibronectin and allow them to attach and spread, while tenascin is anti-adhesive. Further studies using both of these experimental paradigms will clarify whether these interactions also occur in vivo.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1006/cbir.2001.0818" target="_blank" rel="noreferrer noopener">10.1006/cbir.2001.0818</a>
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
2002
Animals
Antisense/*pharmacology
Bhattacharya Raka
Cattle
Cell Adhesion
Cell biology international
Cell Culture Techniques/*methods
Cells
Chick Embryo
Chickens
Complementary/metabolism
Cornea/*cytology
Cultured
DNA
Doane Kathleen J
Dose-Response Relationship
Drug
Fibronectins/*metabolism
Fluorescence
Immunohistochemistry
Integrin beta1/*metabolism/*physiology
Integrins/metabolism
Marchant Jeff
Microscopy
Oligonucleotides
Protein Binding
Retroviridae/genetics
Tenascin/*metabolism