1
40
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Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.5966/sctm.2013-0157" target="_blank" rel="noreferrer noopener">http://doi.org/10.5966/sctm.2013-0157</a>
Pages
760–767
Issue
6
Volume
3
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
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Functional outcome after anal sphincter injury and treatment with mesenchymal stem cells.
Publisher
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Stem cells translational medicine
Date
A point or period of time associated with an event in the lifecycle of the resource
2014
2014-06
Subject
The topic of the resource
Female; Time Factors; Animals; Rats; Mesenchymal stem cells; Transfection; Recovery of Function; Fibrosis; *Mesenchymal Stem Cell Transplantation; *Regeneration; Anal Canal/injuries/metabolism/pathology/physiopathology/*surgery; Anal pressures; Anal sphincter; Fecal incontinence; Green Fluorescent Proteins/biosynthesis/genetics; i.v. infusion; Mesenchymal Stem Cells/metabolism; Pressure; Injections; Intralesional; Sprague-Dawley; Cells; Cultured; Animal; Disease Models; Infusions; Intravenous
Creator
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Salcedo Levilester; Penn Marc; Damaser Margot; Balog Brian; Zutshi Massarat
Description
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This research demonstrates the regenerative effects of mesenchymal stem cells (MSCs) on the injured anal sphincter by comparing anal sphincter pressures following intramuscular and serial intravascular MSC infusion in a rat model of anal sphincter injury. Fifty rats were divided into injury (n = 35) and no injury (NI; n = 15) groups. Each group was further divided into i.m., serial i.v., or no-treatment (n = 5) groups and followed for 5 weeks. The injury consisted of an excision of 25% of the anal sphincter complex. Twenty-four hours after injury, 5 x 10(5) green fluorescent protein-labeled MSCs in 0.2 ml of phosphate-buffered saline (PBS) or PBS alone (sham) were injected into the anal sphincter for i.m. treatment; i.v. and sham i.v. treatments were delivered daily for 6 consecutive days via the tail vein. Anal pressures were recorded before injury and 10 days and 5 weeks after treatment. Ten days after i.m. MSC treatment, resting and peak pressures were significantly increased compared with those in sham i.m. treatment (p \textless .001). When compared with the NI group, the injury groups had anal pressures that were not significantly different 5 weeks after i.m./i.v. treatment. Both resting and peak pressures were also significantly increased after i.m./i.v. MSC treatment compared with treatment with PBS (p \textless .001), suggesting recovery. Statistical analysis was done using paired t test with Bonferroni correction. Marked decrease in fibrosis and scar tissue was seen in both MSC-treated groups. Both i.m. and i.v. MSC treatment after injury caused an increase in anal pressures sustained at 5 weeks, although fewer cells were injected i.m. The
Identifier
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<a href="http://doi.org/10.5966/sctm.2013-0157" target="_blank" rel="noreferrer noopener">10.5966/sctm.2013-0157</a>
Rights
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Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
*Mesenchymal Stem Cell Transplantation
*Regeneration
2014
Anal Canal/injuries/metabolism/pathology/physiopathology/*surgery
Anal pressures
Anal sphincter
Animal
Animals
Balog Brian
Cells
Cultured
Damaser Margot
Disease Models
Fecal incontinence
Female
Fibrosis
Green Fluorescent Proteins/biosynthesis/genetics
i.v. infusion
Infusions
Injections
Intralesional
Intravenous
Mesenchymal stem cells
Mesenchymal Stem Cells/metabolism
Penn Marc
Pressure
Rats
Recovery of Function
Salcedo Levilester
Sprague-Dawley
Stem cells translational medicine
Time Factors
Transfection
Zutshi Massarat
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1002/sctm.17-0046" target="_blank" rel="noreferrer noopener">http://doi.org/10.1002/sctm.17-0046</a>
Pages
1759–1766
Issue
9
Volume
6
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
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A Novel Role for CAMKK1 in the Regulation of the Mesenchymal Stem Cell Secretome.
Publisher
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Stem cells translational medicine
Date
A point or period of time associated with an event in the lifecycle of the resource
2017
2017-09
Subject
The topic of the resource
Calcium/calmodulin-dependent protein kinase kinase-1; Cardiac disease; Cardiac regeneration; Mesenchymal stem cells; Secretome
Creator
An entity primarily responsible for making the resource
Dong Feng; Patnaik Shyam; Duan Zhong-Hui; Kiedrowski Matthew; Penn Marc S; Mayorga Maritza E
Description
An account of the resource
Transplantation of adult stem cells into myocardial tissue after acute myocardial infarction (AMI), has been shown to improve tissue recovery and prevent progression to ischemic cardiomyopathy. Studies suggest that the effects of mesenchymal stem cells (MSC) are due to paracrine factors released by MSC, as the benefits of MSC can be achieved through delivery of conditioned media (CM) alone. We previously demonstrated that downregulation of Dab2 enhances MSC cardiac protein expression and improves cardiac function after AMI following MSC engraftment. In order to define the molecular mechanisms that regulate MSC secretome, we analyzed gene arrays in MSC following downregulation of Dab2 via TGFbeta1 pretreatment or transfection with Dab2:siRNA or miR-145. We identified 23 genes whose expressions were significantly changed in all three conditions. Among these genes, we have initially focused our validation and functional work on calcium/calmodulin-dependent protein kinase kinase-1 (CAMKK1). We quantified the effects of CAMKK1 overexpression in MSC following injection of CM after AMI. Injections of CM from MSC with CAMKK1 over-expression correlated with an increase in vascular density (CAMKK1 CM: 2,794.95 +/- 44.2 versus Control: 1,290.69 +/- 2.8 vessels/mm(2) ) and decreased scar formation (CAMKK1 CM 50% +/- 3.2% versus Control: 28% +/- 1.4%), as well as improved cardiac function. Direct overexpression of CAMKK1 in infarcted tissue using a CAMKK1-encoding plasmid significantly improved ejection fraction (CAMKK1: 83.2% +/- 5.4% versus saline: 51.7% +/- 5.8%. Baseline: 91.3% +/- 4.3%) and decreased infarct size after AMI. Our data identify a novel role for CAMKK1 as regulator of the MSC secretome and demonstrate that direct overexpression of CAMKK1 in infarcted cardiac tissue, results in therapeutic beneficial effects. Stem Cells Translational Medicine 2017;6:1759-1766.
Identifier
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<a href="http://doi.org/10.1002/sctm.17-0046" target="_blank" rel="noreferrer noopener">10.1002/sctm.17-0046</a>
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
2017
Calcium/calmodulin-dependent protein kinase kinase-1
Cardiac disease
Cardiac regeneration
Department of Integrative Medical Sciences
Dong Feng
Duan Zhong-Hui
Kiedrowski Matthew
Mayorga Maritza E
Mesenchymal stem cells
NEOMED College of Medicine
Patnaik Shyam
Penn Marc S
Secretome
Stem cells translational medicine