1
40
2
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1016/0169-328x(93)90070-6" target="_blank" rel="noreferrer noopener">http://doi.org/10.1016/0169-328x(93)90070-6</a>
Pages
36–40
Issue
1
Volume
17
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Para-chlorophenylalanine treatment inhibits the expression of vasoactive intestinal peptide messenger RNA in rat anterior pituitary.
Publisher
An entity responsible for making the resource available
Brain research. Molecular brain research
Date
A point or period of time associated with an event in the lifecycle of the resource
1993
1993-01
Subject
The topic of the resource
alpha-Methyltyrosine; Animals; Anterior/*drug effects/metabolism; Chemical; Depression; Dopamine/metabolism; Fenclonine/*pharmacology; Gene Expression Regulation/drug effects; Male; Messenger/*biosynthesis; Methyltyrosines/pharmacology; Pituitary Gland; Prolactin/metabolism; Rats; RNA; Serotonin/metabolism; Sprague-Dawley; Tryptophan Hydroxylase/antagonists & inhibitors; Vasoactive Intestinal Peptide/*biosynthesis/genetics
Creator
An entity primarily responsible for making the resource
Signs S A; Dluzen D E; Carrillo A J
Description
An account of the resource
Adult male Sprague-Dawley rats were treated with para-chlorophenylalanine (pCPA) or alpha-methyl tyrosine (alpha-MT) to study the effect of serotonin or catecholamine depletion on the expression of vasoactive intestinal peptide (VIP) messenger RNA in the anterior pituitary. Single injections of pCPA (300 mg/kg) for two consecutive days resulted on the third day in a dramatic depletion of serotonin in the medial basal hypothalamus, and a significant reduction in the pituitary content of VIP mRNA (1.0 and 1.7 kb). The effect of pCPA on VIP mRNA appeared to be relatively specific for the anterior pituitary since VIP message levels in the cerebral cortex did not decrease. alpha-MT treatment, (150 mg/kg) for 2 consecutive days, reduced dopamine concentrations in the MBH but had no significant effect on pituitary VIP levels. In a time-course study, hypothalamic serotonin and pituitary VIP mRNA levels were significantly depressed 1-3 days after initiation of pCPA treatment; however, 12 days after pCPA treatment, serotonin concentrations in the hypothalamus approached control values and pituitary VIP mRNA content increased an average of 2-fold over control levels in an apparent rebound effect. pCPA-treated rats injected i.p. twice a day with
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1016/0169-328x(93)90070-6" target="_blank" rel="noreferrer noopener">10.1016/0169-328x(93)90070-6</a>
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
1993
alpha-Methyltyrosine
Animals
Anterior/*drug effects/metabolism
Brain research. Molecular brain research
Carrillo A J
Chemical
Depression
Dluzen D E
Dopamine/metabolism
Fenclonine/*pharmacology
Gene Expression Regulation/drug effects
Male
Messenger/*biosynthesis
Methyltyrosines/pharmacology
Pituitary Gland
Prolactin/metabolism
Rats
RNA
Serotonin/metabolism
Signs S A
Sprague-Dawley
Tryptophan Hydroxylase/antagonists & inhibitors
Vasoactive Intestinal Peptide/*biosynthesis/genetics
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1006/bbrc.1994.1080" target="_blank" rel="noreferrer noopener">http://doi.org/10.1006/bbrc.1994.1080</a>
Pages
546–553
Issue
2
Volume
198
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Effects of bile acids and steroid/thyroid hormones on the expression of cholesterol 7 alpha-hydroxylase mRNA and the CYP7 gene in HepG2 cells.
Publisher
An entity responsible for making the resource available
Biochemical and biophysical research communications
Date
A point or period of time associated with an event in the lifecycle of the resource
1994
1994-01
Subject
The topic of the resource
Animals; Bile Acids and Salts/*pharmacology; Cells; Cholesterol 7-alpha-Hydroxylase/*genetics; Cultured; Enzymologic/*drug effects; Gene Expression Regulation; Genetic; Genetic/genetics; Humans; Luciferases/biosynthesis/genetics; Messenger/*biosynthesis; Promoter Regions; Rats; Recombinant Fusion Proteins/biosynthesis; RNA; Steroids/*pharmacology; Thyroid Hormones/*pharmacology; Transcription; Transfection
Creator
An entity primarily responsible for making the resource
Crestani M; Karam W G; Chiang J Y
Description
An account of the resource
The expression of cholesterol 7 alpha-hydroxylase mRNA levels in confluent HepG2 cultures was reduced by tauro- or glyco-conjugates of deoxycholate and chenodeoxycholate, but not by cholate. Ursodeoxycholates, on the other hand, stimulated the mRNA level. The 5'-upstream regions of rat cholesterol 7 alpha-hydroxylase gene (CYP7) were fused to luciferase reporter gene and the constructs, p-3616/Luc, p-224/Luc and p-160/Luc, were transiently transfected into HepG2 cells. Tauro-conjugates of deoxycholate and chenodeoxycholate inhibited the transcriptional activities of the gene constructs in the confluent cells, but not in subconfluent cells. These results reveal that bile acid responsive elements are located in the -160 fragment and also between nt -3616 and -224. Thyroid and steroid hormones stimulated transcriptional activity expressed in the confluent cells and their responsive elements are located upstream of nt -224. It appears that adult phenotypes are responsible for bile acid feedback and hormone response in HepG2 cells.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1006/bbrc.1994.1080" target="_blank" rel="noreferrer noopener">10.1006/bbrc.1994.1080</a>
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
1994
Animals
Bile Acids and Salts/*pharmacology
Biochemical and biophysical research communications
Cells
Chiang J Y
Cholesterol 7-alpha-Hydroxylase/*genetics
Crestani M
Cultured
Department of Integrative Medical Sciences
Enzymologic/*drug effects
Gene Expression Regulation
Genetic
Genetic/genetics
Humans
Karam W G
Luciferases/biosynthesis/genetics
Messenger/*biosynthesis
NEOMED College of Medicine
Promoter Regions
Rats
Recombinant Fusion Proteins/biosynthesis
RNA
Steroids/*pharmacology
Thyroid Hormones/*pharmacology
Transcription
Transfection