1
40
5
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1016/s0006-291x(88)80881-7" target="_blank" rel="noreferrer noopener">http://doi.org/10.1016/s0006-291x(88)80881-7</a>
Pages
576–580
Issue
1
Volume
156
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
The complete nucleotide sequence of human liver cytochrome b5 mRNA.
Publisher
An entity responsible for making the resource available
Biochemical and biophysical research communications
Date
A point or period of time associated with an event in the lifecycle of the resource
1988
1988-10
Subject
The topic of the resource
Humans; Amino Acid Sequence; Liver/*metabolism; Base Sequence; Molecular Sequence Data; DNA/genetics; Cytochrome b Group/*genetics; Cytochromes b5; RNA; Cloning; Molecular; Messenger/*genetics
Creator
An entity primarily responsible for making the resource
Yoo M; Steggles A W
Description
An account of the resource
We have isolated and sequenced a cDNA clone corresponding to human liver cytochrome b5 mRNA. The 760 base pair (bp) sequence contains the complete coding and 3' non-translated regions plus 52 bp of 5' non-translated sequence. The derived amino acid sequence showed that the previous assignment of several amino acids was in error. In addition, the sequence of the previously unknown COOH hydrophobic region has been obtained.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1016/s0006-291x(88)80881-7" target="_blank" rel="noreferrer noopener">10.1016/s0006-291x(88)80881-7</a>
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
1988
Amino Acid Sequence
Base Sequence
Biochemical and biophysical research communications
Cloning
Cytochrome b Group/*genetics
Cytochromes b5
DNA/genetics
Humans
Liver/*metabolism
Messenger/*genetics
Molecular
Molecular Sequence Data
RNA
Steggles A W
Yoo M
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
Pages
12012–12019
Issue
20
Volume
265
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Regulation of cholesterol 7 alpha-hydroxylase in the liver. Cloning, sequencing, and regulation of cholesterol 7 alpha-hydroxylase mRNA.
Publisher
An entity responsible for making the resource available
The Journal of biological chemistry
Date
A point or period of time associated with an event in the lifecycle of the resource
1990
1990-07
Subject
The topic of the resource
Female; Animals; Rats; Amino Acid Sequence; *Gene Expression Regulation; Kinetics; Base Sequence; Immunoblotting; Molecular Sequence Data; Steroid Hydroxylases/*genetics; Circadian Rhythm; DNA/genetics/isolation & purification; Restriction Mapping; Enzyme Induction; Liver/drug effects/*enzymology; Cell Fractionation; Cholesterol 7-alpha-Hydroxylase/biosynthesis/*genetics/immunology; Cholestyramine Resin/pharmacology; Cytochrome P-450 Enzyme System/genetics; Epitopes/analysis; Polyribosomes/metabolism/ultrastructure; Inbred Strains; RNA; Enzymologic; Sequence Homology; Cloning; Nucleic Acid; Messenger/*genetics; Centrifugation; Density Gradient; Molecular/methods
Creator
An entity primarily responsible for making the resource
Li Y C; Wang D P; Chiang J Y
Description
An account of the resource
Monospecific antibody against purified rat liver cholesterol 7 alpha-hydroxylase cytochrome P-450 was used to screen a lambda gt11 cDNA library constructed from immuno-enriched polysomal RNA of cholestyramine-treated female rat liver. Two types of cDNA clones differing in the length of the 3'-untranslated region were identified, and DNA sequences were determined. The full length clone contains 3561 base pairs plus a long poly(A) tail. The amino acid sequence deduced from the open reading frame revealed a unique P-450 protein containing 503 amino acid residues which belonged to a new gene family designated family VII or CYP7. Southern blot hybridization experiments indicated that the minimal size of P-450 VII gene was 11 kilobase pairs (kb), and there was probably only one gene in this new family. Northern blot hybridization using specific cDNA probes revealed at least two major mRNA species of about 4.0 kb and 2.1 kb, respectively. These two mRNA species may be derived from the use of different polyadenylation signals and reverse-transcribed to two types of cDNA clones. Cholesterol 7 alpha-hydroxylase mRNAs were induced 2- to 3-fold in rat liver by cholestyramine treatment. The mRNA level was rapidly reduced upon the removal of the inducer. Similarly, cholesterol feeding induced enzyme activity, protein, and mRNA levels in the rat by 2-fold, suggesting that cholesterol is an important regulator of cholesterol 7 alpha-hydroxylase in the liver. On the other hand, dexamethasone and pregnenolone-16 alpha-carbonitrile drastically reduced the activity, protein, and mRNA levels. These experiments suggest that the induction of cholesterol 7 alpha-hydroxylase activity by cholestyramine or cholesterol and inhibition of cholesterol 7 alpha-hydroxylase activity by bile acid feedback are results of the rapid turnover of cholesterol 7 alpha-hydroxylase enzyme and mRNA levels.
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
*Gene Expression Regulation
1990
Amino Acid Sequence
Animals
Base Sequence
Cell Fractionation
Centrifugation
Chiang J Y
Cholesterol 7-alpha-Hydroxylase/biosynthesis/*genetics/immunology
Cholestyramine Resin/pharmacology
Circadian Rhythm
Cloning
Cytochrome P-450 Enzyme System/genetics
Density Gradient
Department of Integrative Medical Sciences
DNA/genetics/isolation & purification
Enzyme Induction
Enzymologic
Epitopes/analysis
Female
Immunoblotting
Inbred Strains
Kinetics
Li Y C
Liver/drug effects/*enzymology
Messenger/*genetics
Molecular Sequence Data
Molecular/methods
NEOMED College of Medicine
Nucleic Acid
Polyribosomes/metabolism/ultrastructure
Rats
Restriction Mapping
RNA
Sequence Homology
Steroid Hydroxylases/*genetics
The Journal of biological chemistry
Wang D P
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
Pages
351–353
Issue
5
Volume
1
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
The nucleotide sequence of rabbit liver cytochrome b5 mRNA.
Publisher
An entity responsible for making the resource available
Protein sequences & data analysis
Date
A point or period of time associated with an event in the lifecycle of the resource
1988
1905-06
Subject
The topic of the resource
Animals; Amino Acid Sequence; Liver/*metabolism; Rabbits; Base Sequence; Molecular Sequence Data; Cytochrome b Group/*genetics; Cytochromes b5; Chromosome Deletion; Genes; RNA; Cloning; Molecular; Messenger/*genetics
Creator
An entity primarily responsible for making the resource
Dariush N; Fisher C W; Steggles A W
Description
An account of the resource
Using a synthetic 26 base pair (bp) oligonucleotide, we have screened a rabbit liver cDNA library in lambda gt11 and isolated a 1000 bp DNA clone corresponding to cytochrome b5 mRNA. The clone contains the complete coding region and long 5' and 3' non-translated regions. The derived amino acid sequence (sequence; see text) agrees with published sequences, and confirms that the amino acids 62 and 104 are asparagine and aspartic acid, respectively.
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
1988
Amino Acid Sequence
Animals
Base Sequence
Chromosome Deletion
Cloning
Cytochrome b Group/*genetics
Cytochromes b5
Dariush N
Fisher C W
Genes
Liver/*metabolism
Messenger/*genetics
Molecular
Molecular Sequence Data
Protein sequences & data analysis
Rabbits
RNA
Steggles A W
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
Pages
123–130; discussion 131
Volume
319
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Isolation and characterization of cDNA clones for human, bovine and rabbit liver cytochrome b5 mRNA's.
Publisher
An entity responsible for making the resource available
Progress in clinical and biological research
Date
A point or period of time associated with an event in the lifecycle of the resource
1989
1905-06
Subject
The topic of the resource
Humans; Animals; Amino Acid Sequence; Rabbits; Base Sequence; Molecular Sequence Data; Liver/*enzymology; Cattle; Chickens; Cytochromes b5/blood/*genetics; DNA/*isolation & purification; Erythrocytes/enzymology; RNA; Sequence Homology; Cloning; Molecular; Nucleic Acid; Messenger/*genetics
Creator
An entity primarily responsible for making the resource
Cristiano R J; Yoo M; Dariush N; Steggles A W
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
1989
Amino Acid Sequence
Animals
Base Sequence
Cattle
Chickens
Cloning
Cristiano R J
Cytochromes b5/blood/*genetics
Dariush N
DNA/*isolation & purification
Erythrocytes/enzymology
Humans
Liver/*enzymology
Messenger/*genetics
Molecular
Molecular Sequence Data
Nucleic Acid
Progress in clinical and biological research
Rabbits
RNA
Sequence Homology
Steggles A W
Yoo M
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1016/j.ab.2017.03.009" target="_blank" rel="noreferrer noopener">http://doi.org/10.1016/j.ab.2017.03.009</a>
Pages
29–32
Volume
526
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
An effective and efficient method of transfecting primary human chondrocytes in suspension.
Publisher
An entity responsible for making the resource available
Analytical biochemistry
Date
A point or period of time associated with an event in the lifecycle of the resource
2017
2017-06
Subject
The topic of the resource
*Chondrocytes; *Osteoarthritis; *siRNA; *Transfection; Aggrecans/genetics; Articular/metabolism/pathology; Cartilage; Cells; Chondrocytes/cytology/*metabolism; Collagen Type II/genetics; Cultured; Humans; Messenger/*genetics; mRNA Cleavage and Polyadenylation Factors/antagonists & inhibitors/genetics; Nucleotidyltransferases/antagonists & inhibitors/genetics; Plasmids/*genetics; RNA; Small Interfering/*genetics; Transfection/*methods
Creator
An entity primarily responsible for making the resource
Makki Mohammad Shahidul; Akhtar Nahid; Haqqi Tariq M
Description
An account of the resource
Human chondrocytes accumulate an ECM-rich matrix by secreting matrix macromolecules during monolayer culture, which makes them difficult to transfect efficiently. Here we report a non-viral based protocol to transfect the primary human chondrocytes with high efficiency in suspension. Chondrocyte cultures were digested using Pronase and Collagenase and transfected in suspension. Transfection efficiencies of more than 80% were achieved routinely using the protocol described. The viability of siRNA transfected or un-transfected chondrocytes was not affected and resulted in 80-90% knockdown of the target mRNA levels. This protocol may be useful in gene knockdown, and ectopic overexpression studies in chondrocytes.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1016/j.ab.2017.03.009" target="_blank" rel="noreferrer noopener">10.1016/j.ab.2017.03.009</a>
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
*CHONDROCYTES
*OSTEOARTHRITIS
*siRNA
*Transfection
2017
Aggrecans/genetics
Akhtar Nahid
Analytical biochemistry
Articular/metabolism/pathology
Cartilage
Cells
Chondrocytes/cytology/*metabolism
Collagen Type II/genetics
Cultured
Department of Anatomy & Neurobiology
Haqqi Tariq M
Humans
Makki Mohammad Shahidul
Messenger/*genetics
mRNA Cleavage and Polyadenylation Factors/antagonists & inhibitors/genetics
NEOMED College of Medicine
Nucleotidyltransferases/antagonists & inhibitors/genetics
Plasmids/*genetics
RNA
Small Interfering/*genetics
Transfection/*methods