1
40
20
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1186/s12974-018-1100-1" target="_blank" rel="noreferrer noopener">http://doi.org/10.1186/s12974-018-1100-1</a>
Rights
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
Pages
73-73
Issue
1
Volume
15
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
The glycoprotein GPNMB attenuates astrocyte inflammatory responses through the CD44 receptor.
Publisher
An entity responsible for making the resource available
Journal of neuroinflammation
Date
A point or period of time associated with an event in the lifecycle of the resource
2018
2018-03
Subject
The topic of the resource
Female; Humans; Male; Animals; Mice; Reactive Oxygen Species/metabolism; Astrocyte; CD44; GPNMB; *Neuroinflammation; *Parkinson's disease; Case-Control Studies; Analysis of Variance; Signal Transduction/drug effects; Hyaluronan Receptors/*metabolism; Glial Fibrillary Acidic Protein/metabolism; Nitric Oxide/metabolism; Cells; Cultured; RNA; Messenger/metabolism; Chemical; 3; Databases; 1-Methyl-4-phenyl-1; 2; 6-tetrahydropyridine/pharmacology; Anti-Inflammatory Agents/*therapeutic use; Astrocytes/*drug effects; Cytokines/genetics/metabolism; Inflammation/*drug therapy/etiology; Membrane Glycoproteins/*therapeutic use; Neurotoxins/toxicity; Parkinson Disease/complications/*pathology
Creator
An entity primarily responsible for making the resource
Neal Matthew L; Boyle Alexa M; Budge Kevin M; Safadi Fayez F; Richardson Jason R
Description
An account of the resource
BACKGROUND: Neuroinflammation is one of the hallmarks of neurodegenerative diseases, such as Parkinson's disease (PD). Activation of glial cells, including microglia and astrocytes, is a characteristic of the inflammatory response. Glycoprotein non-metastatic melanoma protein B (GPNMB) is a transmembrane glycoprotein that releases a soluble signaling peptide when cleaved by ADAM10 or other extracellular proteases. GPNMB has demonstrated a neuroprotective role in animal models of ALS and ischemia. However, the mechanism of this protection has not been well established. CD44 is a receptor expressed on astrocytes that can bind GPNMB, and CD44 activation has been demonstrated to reduce NFkappaB activation and subsequent inflammatory responses in macrophages. GPNMB signaling has not been investigated in models of PD or specifically in astrocytes. More recently, genetic studies have linked polymorphisms in GPNMB with risk for PD. Therefore, it is important to understand the role this signaling protein plays in PD. METHODS: We used data mining techniques to evaluate mRNA expression of GPNMB and its receptor CD44 in the substantia nigra of PD and control brains. Immunofluorescence and qPCR techniques were used to assess GPNMB and CD44 levels in mice treated with MPTP. In vitro experiments utilized the immortalized mouse astrocyte cell line IMA2.1 and purified primary mouse astrocytes. The effects of recombinant GPNMB on cytokine-induced astrocyte activation was determined by qPCR, immunofluorescence, and measurement of nitric oxide and reactive oxygen production. RESULTS: Increased GPNMB and CD44 expression was observed in the substantia nigra of human PD brains and in GFAP-positive astrocytes in an animal model of PD. GPNMB treatment attenuated cytokine-induced levels of inducible nitric oxide synthase, nitric oxide, reactive oxygen species, and the inflammatory cytokine IL-6 in an astrocyte cell line and primary mouse astrocytes. Using primary mouse astrocytes from CD44 knockout mice, we found that the anti-inflammatory effects of GPNMB are CD44-mediated. CONCLUSIONS: These results demonstrate that GPNMB may exert its neuroprotective effect through reducing astrocyte-mediated neuroinflammation in a CD44-dependent manner, providing novel mechanistic insight into the neuroprotective properties of GPNMB.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1186/s12974-018-1100-1" target="_blank" rel="noreferrer noopener">10.1186/s12974-018-1100-1</a>
*Neuroinflammation
*Parkinson's disease
1-Methyl-4-phenyl-1
2
2018
3
6-tetrahydropyridine/pharmacology
Analysis of Variance
Animals
Anti-Inflammatory Agents/*therapeutic use
Astrocyte
Astrocytes/*drug effects
Boyle Alexa M
Budge Kevin M
Case-Control Studies
CD44
Cells
Chemical
Cultured
Cytokines/genetics/metabolism
Databases
Female
Glial Fibrillary Acidic Protein/metabolism
GPNMB
Humans
Hyaluronan Receptors/*metabolism
Inflammation/*drug therapy/etiology
Journal of neuroinflammation
Male
Membrane Glycoproteins/*therapeutic use
Messenger/metabolism
Mice
Neal Matthew L
Neurotoxins/toxicity
Nitric Oxide/metabolism
Parkinson Disease/complications/*pathology
Reactive Oxygen Species/metabolism
Richardson Jason R
RNA
Safadi Fayez F
Signal Transduction/drug effects
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1016/0303-7207(89)90170-6" target="_blank" rel="noreferrer noopener">http://doi.org/10.1016/0303-7207(89)90170-6</a>
Pages
103–109
Issue
1
Volume
65
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Obesity- and sex-related alterations in growth hormone messenger RNA levels.
Publisher
An entity responsible for making the resource available
Molecular and cellular endocrinology
Date
A point or period of time associated with an event in the lifecycle of the resource
1989
1989-08
Subject
The topic of the resource
Female; Male; Animals; Sex Characteristics; Rats; Nucleic Acid Hybridization; DNA Probes; Obesity/*genetics/metabolism; Prolactin/genetics; Somatomedins/*genetics; RNA; Messenger/metabolism; Zucker
Creator
An entity primarily responsible for making the resource
Ahmad I; Steggles A W; Carrillo A J; Finkelstein J A
Description
An account of the resource
The secretion of growth hormone (GH) is abnormal in genetically obese Zucker rats. Measurements of pulsatile GH release and circulating GH levels in lean (Fa/?) and obese (fa/fa) rats have shown that both are reduced in the latter. We have studied pituitary GH gene expression in order to understand the role of GH synthesis in this abnormality. Obese animals have lower pituitary GH mRNA levels than lean controls. Within each genotype a sex difference was observed with the female animals having lower GH mRNA levels than the males. It is unlikely that the GH abnormality is due to a generalized pituitary defect because prolactin mRNA levels were the same in all four groups of rats.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1016/0303-7207(89)90170-6" target="_blank" rel="noreferrer noopener">10.1016/0303-7207(89)90170-6</a>
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Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
1989
Ahmad I
Animals
Carrillo A J
DNA Probes
Female
Finkelstein J A
Male
Messenger/metabolism
Molecular and cellular endocrinology
Nucleic Acid Hybridization
Obesity/*genetics/metabolism
Prolactin/genetics
Rats
RNA
Sex Characteristics
Somatomedins/*genetics
Steggles A W
Zucker
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.2174/138920112798868575" target="_blank" rel="noreferrer noopener">http://doi.org/10.2174/138920112798868575</a>
Pages
229–234
Issue
1
Volume
13
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Alteration of hepatic proinflammatory cytokines is involved in the resveratrol-mediated chemoprevention of chemically-induced hepatocarcinogenesis.
Publisher
An entity responsible for making the resource available
Current pharmaceutical biotechnology
Date
A point or period of time associated with an event in the lifecycle of the resource
2012
2012-01
Subject
The topic of the resource
Female; Animals; Rats; Gene Expression Regulation/drug effects; Liver/drug effects/metabolism; Resveratrol; Diethylnitrosamine; Anticarcinogenic Agents/pharmacology/*therapeutic use; Cytokines/genetics/*metabolism; Phenobarbital; Stilbenes/pharmacology/*therapeutic use; Sprague-Dawley; RNA; Messenger/metabolism; Liver Neoplasms; Experimental/chemically induced/metabolism/*prevention & control
Creator
An entity primarily responsible for making the resource
Mbimba Thomas; Awale Prabha; Bhatia Deepak; Geldenhuys Werner J; Darvesh Altaf S; Carroll Richard T; Bishayee Anupam
Description
An account of the resource
Hepatocellular carcinoma (HCC), one of the most common cancers in the world, is a leading cause of cancerrelated mortality. HCC develops most frequently in the background of oxidative stress and chronic hepatic inflammation due to viral infections, alcohol abuse as well as exposure to environmental and dietary carcinogens. As the prognosis of HCC is extremely poor and mostly unresponsive to current chemotherapeutic treatment regimens, novel preventive approaches like chemoprevention are urgently needed. We have recently found that resveratrol, a dietary polyphenol present in grapes, berries, peanuts as well as red wine, prevents diethylnitrosamine (DENA)-initiated hepatocarcinogenesis in rats through suppression of inflammation and oxidative stress. As cytokines are considered to be important mediators of inflammation, the objective of the present study was to investigate the effects of resveratrol on hepatic cytokines during DENA-initiated hepatocarcinogenesis in rats. Liver samples were harvested from our previous study in which resveratrol (50, 100 and 300 mg/kg) was found to exert a chemopreventive action against rat liver tumorigenesis induced by DENA. The levels of proinflammatory cytokines, namely tumor necrosis factor-alpha (TNF-alpha), interleukin-1beta (IL-1beta) and interleukin- 6 (IL-6), were measured using enzyme-linked immunosorbent assays. The mRNA expression of these cytokines was studied by reverse transcriptase-polymerase chain reaction for comparison. Resveratrol treatment reversed the DENAinduced alteration of the level and expression of hepatic TNF-alpha, IL-1beta and IL-6. From the current results in conjunction with our previous findings, it can be concluded that resveratrol-mediated chemoprevention of rat liver carcinogenesis is related to alteration of proinflammatory cytokines.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.2174/138920112798868575" target="_blank" rel="noreferrer noopener">10.2174/138920112798868575</a>
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
2012
Animals
Anticarcinogenic Agents/pharmacology/*therapeutic use
Awale Prabha
Bhatia Deepak
Bishayee Anupam
Carroll Richard T
Current pharmaceutical biotechnology
Cytokines/genetics/*metabolism
Darvesh Altaf S
Department of Pharmaceutical Sciences
Diethylnitrosamine
Experimental/chemically induced/metabolism/*prevention & control
Female
Geldenhuys Werner J
Gene Expression Regulation/drug effects
Liver Neoplasms
Liver/drug effects/metabolism
Mbimba Thomas
Messenger/metabolism
NEOMED College of Pharmacy
Phenobarbital
Rats
Resveratrol
RNA
Sprague-Dawley
Stilbenes/pharmacology/*therapeutic use
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1194/jlr.M002782" target="_blank" rel="noreferrer noopener">http://doi.org/10.1194/jlr.M002782</a>
Pages
832–842
Issue
4
Volume
51
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Glucose stimulates cholesterol 7alpha-hydroxylase gene transcription in human hepatocytes.
Publisher
An entity responsible for making the resource available
Journal of lipid research
Date
A point or period of time associated with an event in the lifecycle of the resource
2010
2010-04
Subject
The topic of the resource
*Gene Expression Regulation; Acetylation; AMP-Activated Protein Kinases/metabolism; ATP Citrate (pro-S)-Lyase/genetics/metabolism; Bile Acids and Salts/metabolism; Cells; Cholesterol 7-alpha-Hydroxylase/genetics/*metabolism; Cultured; DNA-Binding Proteins/metabolism; Enzymologic; Epigenesis; Genes; Genetic; Glucose/*administration & dosage; Hep G2 Cells; Hepatocyte Nuclear Factor 4/metabolism; Hepatocytes/*enzymology/metabolism; Histones/metabolism; Humans; Hyperglycemia/enzymology/*metabolism; Messenger/metabolism; Methylation; Reporter; RNA; RNA Interference
Creator
An entity primarily responsible for making the resource
Li Tiangang; Chanda Dipanjan; Zhang Yanqiao; Choi Hueng-Sik; Chiang John Y L
Description
An account of the resource
Bile acids play important roles in the regulation of lipid, glucose, and energy homeostasis. Recent studies suggest that glucose regulates gene transcription in the liver. The aim of this study was to investigate the potential role of glucose in regulation of bile acid synthesis in human hepatocytes. High glucose stimulated bile acid synthesis and induced mRNA expression of cholesterol 7alpha-hydroxylase (CYP7A1), the key regulatory gene in bile acid synthesis. Activation of an AMP-activated protein kinase (AMPK) decreased CYP7A1 mRNA, hepatocyte nuclear factor 4alpha (HNF4alpha) protein, and binding to CYP7A1 chromatin. Glucose increased ATP levels to inhibit AMPK and induce HNF4alpha to stimulate CYP7A1 gene transcription. Furthermore, glucose increased histone acetylation and decreased H3K9 di- and tri-methylation in the CYP7A1 chromatin. Knockdown of ATP-citrate lyase, which converts citrate to acetyl-CoA, decreased histone acetylation and attenuated glucose induction of CYP7A1 mRNA expression. These results suggest that glucose signaling also induces CYP7A1 gene transcription by epigenetic regulation of the histone acetylation status. This study uncovers a novel link between hepatic glucose metabolism and bile acid synthesis. Glucose induction of bile acid synthesis may have an important implication in metabolic control of glucose, lipid, and energy homeostasis under normal and diabetic conditions.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1194/jlr.M002782" target="_blank" rel="noreferrer noopener">10.1194/jlr.M002782</a>
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
*Gene Expression Regulation
2010
Acetylation
AMP-Activated Protein Kinases/metabolism
ATP Citrate (pro-S)-Lyase/genetics/metabolism
Bile Acids and Salts/metabolism
Cells
Chanda Dipanjan
Chiang John Y L
Choi Hueng-Sik
Cholesterol 7-alpha-Hydroxylase/genetics/*metabolism
Cultured
Department of Integrative Medical Sciences
DNA-Binding Proteins/metabolism
Enzymologic
Epigenesis
Genes
Genetic
Glucose/*administration & dosage
Hep G2 Cells
Hepatocyte Nuclear Factor 4/metabolism
Hepatocytes/*enzymology/metabolism
Histones/metabolism
Humans
Hyperglycemia/enzymology/*metabolism
Journal of lipid research
Li Tiangang
Messenger/metabolism
Methylation
NEOMED College of Medicine
Reporter
RNA
RNA Interference
Zhang Yanqiao
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1186/1465-9921-9-55" target="_blank" rel="noreferrer noopener">http://doi.org/10.1186/1465-9921-9-55</a>
Pages
55–55
Volume
9
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
RNA interference for CFTR attenuates lung fluid absorption at birth in rats.
Publisher
An entity responsible for making the resource available
Respiratory research
Date
A point or period of time associated with an event in the lifecycle of the resource
2008
2008-07
Subject
The topic of the resource
*RNA Interference; Animals; Biological Transport/drug effects; Cells; Cultured; Cystic Fibrosis Transmembrane Conductance Regulator/*genetics/*metabolism; Epithelial Sodium Channels/genetics/metabolism; Female; Gene Expression Regulation/drug effects; Lung/cytology/drug effects/*metabolism; Messenger/metabolism; Newborn; Pregnancy; Rats; Respiratory Mucosa/cytology/drug effects/metabolism; RNA; Small Interfering/*pharmacology; Sprague-Dawley; Water/*metabolism
Creator
An entity primarily responsible for making the resource
Li Tianbo; Koshy Shyny; Folkesson Hans G
Description
An account of the resource
BACKGROUND: Small interfering RNA (siRNA) against alphaENaC (alpha-subunit of the epithelial Na channel) and CFTR (cystic fibrosis transmembrane conductance regulator) was used to explore ENaC and CTFR function in newborn rat lungs. METHODS: Twenty-four hours after trans-thoracic intrapulmonary (ttip) injection of siRNA-generating plasmid DNA (pSi-0, pSi-4, or pSi-C2), we measured CFTR and ENaC expression, extravascular lung water, and mortality. RESULTS: alphaENaC and CFTR mRNA and protein decreased by approximately 80% and approximately 85%, respectively, following alphaENaC and CFTR silencing. Extravascular lung water and mortality increased after alphaENaC and CFTR-silencing. In pSi-C2-transfected isolated DLE cells there were attenuated CFTR mRNA and protein. In pSi-4-transfected DLE cells alphaENaC mRNA and protein were both reduced. Interestingly, CFTR-silencing also reduced alphaENaC mRNA and protein. alphaENaC silencing, on the other hand, only slightly reduced CFTR mRNA and protein. CONCLUSION: Thus, ENaC and CFTR are both involved in the fluid secretion to absorption conversion around at birth.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1186/1465-9921-9-55" target="_blank" rel="noreferrer noopener">10.1186/1465-9921-9-55</a>
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
*RNA Interference
2008
Animals
Biological Transport/drug effects
Cells
Cultured
Cystic Fibrosis Transmembrane Conductance Regulator/*genetics/*metabolism
Epithelial Sodium Channels/genetics/metabolism
Female
Folkesson Hans G
Gene Expression Regulation/drug effects
Koshy Shyny
Li Tianbo
Lung/cytology/drug effects/*metabolism
Messenger/metabolism
Newborn
Pregnancy
Rats
Respiratory Mucosa/cytology/drug effects/metabolism
Respiratory research
RNA
Small Interfering/*pharmacology
Sprague-Dawley
Water/*metabolism
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1080/03008200390152052" target="_blank" rel="noreferrer noopener">http://doi.org/10.1080/03008200390152052</a>
Pages
28–32
Volume
44 Suppl 1
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Analysis of osteopontin in mouse growth plate cartilage by application of laser capture microdissection and RT-PCR.
Publisher
An entity responsible for making the resource available
Connective tissue research
Date
A point or period of time associated with an event in the lifecycle of the resource
2003
1905-06
Subject
The topic of the resource
*Reverse Transcriptase Polymerase Chain Reaction; Animals; Articular/chemistry/cytology; Cartilage; Chondrocytes/chemistry; Gene Expression; Growth Plate/*chemistry/cytology; Inbred C57BL; Laser Therapy; Messenger/metabolism; Mice; Microdissection/*methods; Newborn; Non-programmatic; Osteopontin; RNA; Sialoglycoproteins/*analysis/genetics; Tibia
Creator
An entity primarily responsible for making the resource
Landis William J; Jacquet Robin; Hillyer Jennifer; Zhang Jean
Description
An account of the resource
Gene expression of osteopontin (OPN) has been investigated in mice by application of laser capture microdissection (LCM) and reverse transcriptase-polymerase chain reaction (RT-PCR) analysis. LCM permits individual cells to be isolated ("captured") from tissue sections for molecular analyses. In this study, chondrocytes were captured from growth plate zones in frozen sections of tibiae from 1-11-day-old postnatal mice. RNA was extracted from cells, DNAse-treated, and reverse-transcribed. cDNA was amplified by PCR and OPN mRNA was revealed on agarose gels. Whole cartilage and brain (a positive control) from the same animals also were examined. Reactions containing no RT were negative controls, and 18S rRNA standardized expressed message from captured cells. RT-PCR analysis of laser-captured whole cartilage showed a general qualitative loss of OPN mRNA as animal age increased. Youngest mice gave equivalent OPN expression over all laser-microdissected cartilage zones. For 7-11 day-old mice, OPN expression was qualitatively greatest in resting and lowest in hypertrophic regions of cartilage. Expression of OPN correlated with mineral in the tissue suggests that OPN functionally may inhibit normal vertebrate growth plate mineralization, and its loss with increasing tissue maturation appears permissive to mineral development.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1080/03008200390152052" target="_blank" rel="noreferrer noopener">10.1080/03008200390152052</a>
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
*Reverse Transcriptase Polymerase Chain Reaction
2003
Animals
Articular/chemistry/cytology
Cartilage
Chondrocytes/chemistry
Connective tissue research
Gene Expression
Growth Plate/*chemistry/cytology
Hillyer Jennifer
Inbred C57BL
Jacquet Robin
Landis William J
Laser Therapy
Messenger/metabolism
Mice
Microdissection/*methods
Newborn
Non-programmatic
Osteopontin
RNA
Sialoglycoproteins/*analysis/genetics
Tibia
Zhang Jean
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1074/jbc.M513420200" target="_blank" rel="noreferrer noopener">http://doi.org/10.1074/jbc.M513420200</a>
Pages
10081–10088
Issue
15
Volume
281
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
A Prospero-related homeodomain protein is a novel co-regulator of hepatocyte nuclear factor 4alpha that regulates the cholesterol 7alpha-hydroxylase gene.
Publisher
An entity responsible for making the resource available
The Journal of biological chemistry
Date
A point or period of time associated with an event in the lifecycle of the resource
2006
2006-04
Subject
The topic of the resource
*Gene Expression Regulation; Aged; Amino Acid Motifs; Bile Acids and Salts/metabolism; Cell Line; Cell Nucleus/metabolism; Cells; Cholesterol 7-alpha-Hydroxylase/*chemistry/*genetics; Cultured/metabolism; Enzymologic; Female; Genes; Genetic; Gluconeogenesis; Glutathione Transferase/metabolism; Hepatocyte Nuclear Factor 4/metabolism/*physiology; Hepatocytes/metabolism; Homeodomain Proteins/metabolism/*physiology; Humans; Immunoprecipitation; Liver/metabolism; Luciferases/metabolism; Male; Messenger/metabolism; Middle Aged; Phosphoenolpyruvate Carboxykinase (ATP)/metabolism; Plasmids/metabolism; Protein Structure; Reporter; Response Elements; Reverse Transcriptase Polymerase Chain Reaction; RNA; Small Interfering/metabolism; Tertiary; Time Factors; Transcription; Transcriptional Activation; Transfection; Tumor Suppressor Proteins; Two-Hybrid System Techniques
Creator
An entity primarily responsible for making the resource
Song Kwang-Hoon; Li Tiangang; Chiang John Y L
Description
An account of the resource
Prox1, an early specific marker for developing liver and pancreas in foregut endoderm has recently been shown to interact with alpha-fetoprotein transcription factor and repress cholesterol 7alpha-hydroxylase (CYP7A1) gene transcription. Using a yeast two-hybrid assay, we found that Prox1 strongly and specifically interacted with hepatocyte nuclear factor (HNF)4alpha, an important transactivator of the human CYP7A1 gene in bile acid synthesis and phosphoenolpyruvate carboxykinase (PEPCK) gene in gluconeogenesis. A real time PCR assay detected Prox1 mRNA expression in human primary hepatocytes and HepG2 cells. Reporter assay, GST pull-down, co-immunoprecipitation, and yeast two-hybrid assays identified a specific interaction between the N-terminal LXXLL motif of Prox1 and the activation function 2 domain of HNF4alpha. Prox1 strongly inhibited HNF4alpha and peroxisome proliferators-activated receptor gamma coactivator-1alpha co-activation of the CYP7A1 and PEPCK genes. Knock down of the endogenous Prox1 by small interfering RNA resulted in significant increase of CYP7A1 and PEPCK mRNA expression and the rate of bile acid synthesis in HepG2 cells. These results suggest that Prox1 is a novel co-regulator of HNF4alpha that may play a key role in the regulation of bile acid synthesis and gluconeogenesis in the liver.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1074/jbc.M513420200" target="_blank" rel="noreferrer noopener">10.1074/jbc.M513420200</a>
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
*Gene Expression Regulation
2006
Aged
Amino Acid Motifs
Bile Acids and Salts/metabolism
Cell Line
Cell Nucleus/metabolism
Cells
Chiang John Y L
Cholesterol 7-alpha-Hydroxylase/*chemistry/*genetics
Cultured/metabolism
Department of Integrative Medical Sciences
Enzymologic
Female
Genes
Genetic
Gluconeogenesis
Glutathione Transferase/metabolism
Hepatocyte Nuclear Factor 4/metabolism/*physiology
Hepatocytes/metabolism
Homeodomain Proteins/metabolism/*physiology
Humans
Immunoprecipitation
Li Tiangang
Liver/metabolism
Luciferases/metabolism
Male
Messenger/metabolism
Middle Aged
NEOMED College of Medicine
Phosphoenolpyruvate Carboxykinase (ATP)/metabolism
Plasmids/metabolism
Protein Structure
Reporter
Response Elements
Reverse Transcriptase Polymerase Chain Reaction
RNA
Small Interfering/metabolism
Song Kwang-Hoon
Tertiary
The Journal of biological chemistry
Time Factors
Transcription
Transcriptional Activation
Transfection
Tumor Suppressor Proteins
Two-Hybrid System Techniques
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1074/jbc.M502751200" target="_blank" rel="noreferrer noopener">http://doi.org/10.1074/jbc.M502751200</a>
Pages
30517–30525
Issue
34
Volume
280
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Bcl-2 positively regulates Sox9-dependent chondrocyte gene expression by suppressing the MEK-ERK1/2 signaling pathway.
Publisher
An entity responsible for making the resource available
The Journal of biological chemistry
Date
A point or period of time associated with an event in the lifecycle of the resource
2005
2005-08
Subject
The topic of the resource
*Gene Expression Regulation; Adenoviridae/genetics; Animals; Apoptosis; beta-Galactosidase/metabolism; Blotting; Butadienes/pharmacology; Caspase Inhibitors; Cell Differentiation; Cell Line; Chondrocytes/*metabolism; Collagen Type II/metabolism; Down-Regulation; Enzyme Inhibitors/pharmacology; Fibroblasts/metabolism; Fluorescence; Genetic; High Mobility Group Proteins/*metabolism; Lac Operon; Luciferases/metabolism; MAP Kinase Kinase Kinases/*metabolism; Messenger/metabolism; Microscopy; Mitogen-Activated Protein Kinase 1/*metabolism; Mitogen-Activated Protein Kinase 3/*metabolism; NF-kappa B/metabolism; Nitriles/pharmacology; Phenotype; Phosphorylation; Promoter Regions; Protein Kinase C-alpha; Protein Kinase C/antagonists & inhibitors; Proteoglycans/metabolism; Proto-Oncogene Proteins c-bcl-2/*metabolism; Rats; Reverse Transcriptase Polymerase Chain Reaction; RNA; Signal Transduction; Small Interfering/metabolism; SOX9 Transcription Factor; Sprague-Dawley; Time Factors; Transcription; Transcription Factors/*metabolism; Transfection; Western
Creator
An entity primarily responsible for making the resource
Yagi Rieko; McBurney Denise; Horton Walter E Jr
Description
An account of the resource
Bcl-2 is an anti-apoptotic protein that has recently been shown to regulate other cellular functions. We previously reported that Bcl-2 regulates chondrocyte matrix gene expression, independent of its anti-apoptotic function. Here, we further investigate this novel function of Bcl-2 and examine three intracellular signaling pathways likely to be associated with this function. The present study demonstrates that the activity of Sox9, a master transcription factor that regulates the gene expression of chondrocyte matrix proteins, is suppressed by Bcl-2 small interference RNA in the presence of caspase inhibitors. This effect was attenuated by prior exposure of chondrocytes to an adenoviral vector expressing sense Bcl-2. In addition, the down-regulation of Bcl-2, Sox9, and chondrocyte-specific gene expression by serum withdrawal in primary chondrocytes was reversed by expressing Bcl-2. Inhibition of the protein kinase C alpha and NFkappaB pathways had no effect on the maintenance of Sox9-dependent gene expression by Bcl-2. In contrast, whereas the MEK-ERK1/2 pathway negatively regulated the differentiated phenotype in wild type chondrocytes, inhibition of this pathway reversed the loss of differentiation markers and fibroblastic phenotype in Bcl-2-deficient chondrocytes. In conclusion, the present study identifies a specific signaling pathway, namely, MEK-ERK1/2, that is downstream of Bcl-2 in the regulation of Sox9-dependent chondrocyte gene expression and phenotype.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1074/jbc.M502751200" target="_blank" rel="noreferrer noopener">10.1074/jbc.M502751200</a>
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
*Gene Expression Regulation
2005
Adenoviridae/genetics
Animals
Apoptosis
beta-Galactosidase/metabolism
Blotting
Butadienes/pharmacology
Caspase Inhibitors
Cell Differentiation
Cell Line
Chondrocytes/*metabolism
Collagen Type II/metabolism
Department of Anatomy & Neurobiology
Down-Regulation
Enzyme Inhibitors/pharmacology
Fibroblasts/metabolism
Fluorescence
Genetic
High Mobility Group Proteins/*metabolism
Horton Walter E Jr
Lac Operon
Luciferases/metabolism
MAP Kinase Kinase Kinases/*metabolism
McBurney Denise
Messenger/metabolism
Microscopy
Mitogen-Activated Protein Kinase 1/*metabolism
Mitogen-Activated Protein Kinase 3/*metabolism
NEOMED College of Medicine
NF-kappa B/metabolism
Nitriles/pharmacology
Phenotype
Phosphorylation
Promoter Regions
Protein Kinase C-alpha
Protein Kinase C/antagonists & inhibitors
Proteoglycans/metabolism
Proto-Oncogene Proteins c-bcl-2/*metabolism
Rats
Reverse Transcriptase Polymerase Chain Reaction
RNA
Signal Transduction
Small Interfering/metabolism
SOX9 Transcription Factor
Sprague-Dawley
The Journal of biological chemistry
Time Factors
Transcription
Transcription Factors/*metabolism
Transfection
Western
Yagi Rieko
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1053/j.gastro.2007.08.042" target="_blank" rel="noreferrer noopener">http://doi.org/10.1053/j.gastro.2007.08.042</a>
Pages
1660–1669
Issue
5
Volume
133
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
A novel role of transforming growth factor beta1 in transcriptional repression of human cholesterol 7alpha-hydroxylase gene.
Publisher
An entity responsible for making the resource available
Gastroenterology
Date
A point or period of time associated with an event in the lifecycle of the resource
2007
2007-11
Subject
The topic of the resource
Bile Acids and Salts/metabolism; Carcinoma; Cell Line; Cells; Cholesterol 7-alpha-Hydroxylase/*genetics/*metabolism; Cultured; Enzyme Inhibitors/pharmacology; Genetic/drug effects/*physiology; Hepatocellular/*metabolism/pathology; Hepatocyte Nuclear Factor 4/metabolism; Hepatocytes/drug effects/*metabolism/pathology; Humans; Hydroxamic Acids/pharmacology; Liver Neoplasms/*metabolism/pathology; Messenger/metabolism; RNA; Signal Transduction/physiology; Smad3 Protein/metabolism; Transcription; Transforming Growth Factor beta1/*metabolism; Tumor
Creator
An entity primarily responsible for making the resource
Li Tiangang; Chiang John Y L
Description
An account of the resource
BACKGROUND & AIMS: Inhibition of cholesterol 7alpha-hydroxylase (CYP7A1) by bile acids and inflammatory cytokines provides an important mechanism to protect hepatocytes from bile acid toxicity during cholestasis. Transforming growth factor beta1 (TGFbeta1) released by hepatic stellate cells during chronic liver injury plays a critical role in liver inflammation and fibrogenesis. The objective of this study is to investigate the role of TGFbeta1 in hepatic bile acid synthesis. METHODS: mRNA expressions in primary human hepatocytes and HepG2 cells were measured by quantitative real-time polymerase chain reaction. Reporter assay, glutathione-S-transferase pull-down assay, adenovirus-mediated gene transduction, and chromatin immunoprecipitation assay were used to study the mechanism of TGFbeta1 regulation of CYP7A1 gene transcription. RESULTS: TGFbeta1 inhibited the mRNA expression of CYP7A1 and bile acid synthesis in HepG2 cells and primary human hepatocytes. Mothers against decapentaplegic homolog (Smad3) inhibited both CYP7A1 promoter activity and mRNA expression by inhibiting
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1053/j.gastro.2007.08.042" target="_blank" rel="noreferrer noopener">10.1053/j.gastro.2007.08.042</a>
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
2007
Bile Acids and Salts/metabolism
Carcinoma
Cell Line
Cells
Chiang John Y L
Cholesterol 7-alpha-Hydroxylase/*genetics/*metabolism
Cultured
Department of Integrative Medical Sciences
Enzyme Inhibitors/pharmacology
Gastroenterology
Genetic/drug effects/*physiology
Hepatocellular/*metabolism/pathology
Hepatocyte Nuclear Factor 4/metabolism
Hepatocytes/drug effects/*metabolism/pathology
Humans
Hydroxamic Acids/pharmacology
Li Tiangang
Liver Neoplasms/*metabolism/pathology
Messenger/metabolism
NEOMED College of Medicine
RNA
Signal Transduction/physiology
Smad3 Protein/metabolism
Transcription
Transforming Growth Factor beta1/*metabolism
Tumor
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1016/s0196-9781(01)00593-9" target="_blank" rel="noreferrer noopener">http://doi.org/10.1016/s0196-9781(01)00593-9</a>
Pages
167–183
Issue
1
Volume
23
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Sensory nerves and neuropeptides in uterine cervical ripening.
Publisher
An entity responsible for making the resource available
Peptides
Date
A point or period of time associated with an event in the lifecycle of the resource
2002
2002-01
Subject
The topic of the resource
Animal; Animals; Calcitonin Gene-Related Peptide/biosynthesis; Capsaicin/pharmacology; Cervical Ripening/*metabolism; Cervix Uteri/*innervation/*metabolism; Complementary/metabolism; Female; Genetic; Immunohistochemistry; Labor; Messenger/metabolism; Neurokinin-1/biosynthesis; Neurons/metabolism; Neuropeptides/*biosynthesis; Nitric Oxide Synthase/biosynthesis; Obstetric; Plasmids/metabolism; Postpartum Period; Pregnancy; Rats; Receptors; RNA; Secretogranin II; Sprague-Dawley; Substance P/biosynthesis; Transcription
Creator
An entity primarily responsible for making the resource
Collins J J; Usip S; McCarson K E; Papka R E
Description
An account of the resource
At the time of parturition (fetal delivery) the uterine cervix must "ripen," becoming soft, pliable, and dilated to accommodate the fetus' delivery. The fundamental processes underlying cervical ripening remain poorly understood. Knowledge that abundant autonomic and sensory nerves supply the uterine cervix, that transection of afferent nerves supplying the cervix blocks parturition, and that some of the changes in the cervix resemble those seen in inflammatory reactions suggests nerves may have a role in the cervical ripening changes. The present study utilized immunohistochemistry, plasma extravasation, and solution hybridization-nuclease protection assay to elucidate the complement of primary afferent nerves and some receptors in the rat cervix during pregnancy, and to determine if they may have roles in the ripening process at term. This study revealed an abundance of nerves associated with the cervical vasculature and myometrial smooth muscle containing immunoreactivity for substance P, calcitonin gene-related peptide, secretoneurin, and nitric oxide synthase throughout pregnancy. Many of these are small unmyelinated capsaicin-sensitive C-fibers. Substance P- (NK1-) and calcitonin gene-related peptide receptors were apparent on uterine cervix vasculature from pregnant, parturient, and postpartum rats. NK1 receptor mRNA was maximal at 20 days of pregnancy. Plasma extravasation of i.v. administered Evans Blue or Monastral Blue was most pronounced at parturition (shortly after NK1 mRNA is maximal); this was similar to plasma extravasation evoked by i.v. administration of substance P or capsaicin-treatment. This study revealed new data about the nervous system of the rat uterine cervix and that these nerves and their transmitters could very well be part of a neurogenic inflammatory process involved in cervical ripening.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1016/s0196-9781(01)00593-9" target="_blank" rel="noreferrer noopener">10.1016/s0196-9781(01)00593-9</a>
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
2002
Animal
Animals
Calcitonin Gene-Related Peptide/biosynthesis
Capsaicin/pharmacology
Cervical Ripening/*metabolism
Cervix Uteri/*innervation/*metabolism
Collins J J
Complementary/metabolism
Department of Anatomy & Neurobiology
Female
Genetic
Immunohistochemistry
Labor
McCarson K E
Messenger/metabolism
NEOMED College of Medicine
Neurokinin-1/biosynthesis
Neurons/metabolism
Neuropeptides/*biosynthesis
Nitric Oxide Synthase/biosynthesis
Obstetric
Papka R E
Peptides
Plasmids/metabolism
Postpartum Period
Pregnancy
Rats
Receptors
RNA
Secretogranin II
Sprague-Dawley
Substance P/biosynthesis
Transcription
Usip S
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1016/s0168-1702(01)00257-x" target="_blank" rel="noreferrer noopener">http://doi.org/10.1016/s0168-1702(01)00257-x</a>
Pages
127–135
Issue
2
Volume
76
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Changes in BiP (GRP78) levels upon HSV-1 infection are strain dependent.
Publisher
An entity responsible for making the resource available
Virus research
Date
A point or period of time associated with an event in the lifecycle of the resource
2001
2001-08
Subject
The topic of the resource
3T3 Cells; Animals; Carrier Proteins/*biosynthesis/genetics; Heat-Shock Proteins/*biosynthesis/genetics; Herpesvirus 1; Human/isolation & purification/*physiology; Humans; Messenger/metabolism; Mice; Molecular Chaperones/*biosynthesis/genetics; RNA
Creator
An entity primarily responsible for making the resource
Mao H; Palmer D; Rosenthal K S
Description
An account of the resource
BiP (grp78) is a chaperone protein which can also regulate the unfolded protein response of the cell. Levels of BiP increased in cells infected by the small plaque producing, cell associated, neuroinvasive strains of HSV-1 (SP7, 490) but decreased in cells infected with KOS, a large plaque, attenuated strain. BiP protein synthesis continued early in infection and BiP was sequestered and its degradation was limited during SP7 infection. BiP protein synthesis stopped and the protein was degraded in KOS infected cells. These viral strain dependent differences in BiP concentration may influence other aspects of the viral interaction with the target cell and its host.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1016/s0168-1702(01)00257-x" target="_blank" rel="noreferrer noopener">10.1016/s0168-1702(01)00257-x</a>
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
2001
3T3 Cells
Animals
Carrier Proteins/*biosynthesis/genetics
Heat-Shock Proteins/*biosynthesis/genetics
Herpesvirus 1
Human/isolation & purification/*physiology
Humans
Mao H
Messenger/metabolism
Mice
Molecular Chaperones/*biosynthesis/genetics
Palmer D
RNA
Rosenthal K S
Virus research
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1016/j.peptides.2003.07.009" target="_blank" rel="noreferrer noopener">http://doi.org/10.1016/j.peptides.2003.07.009</a>
Pages
1163–1174
Issue
8
Volume
24
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
The effects of pregnancy and estrogen on the expression of calcitonin gene-related peptide (CGRP) in the uterine cervix, dorsal root ganglia and spinal cord.
Publisher
An entity responsible for making the resource available
Peptides
Date
A point or period of time associated with an event in the lifecycle of the resource
2003
2003-08
Subject
The topic of the resource
Animals; Calcitonin Gene-Related Peptide/analysis/biosynthesis/*genetics; Cervix Uteri/*physiology; Estrogens/*physiology; Female; Ganglia; Immunohistochemistry; In Situ Hybridization; Messenger/metabolism; Pregnancy; Radioimmunoassay; Rats; RNA; Spinal Cord/*physiology; Spinal/*physiology; Sprague-Dawley
Creator
An entity primarily responsible for making the resource
Mowa C N; Usip S; Collins J; Storey-Workley M; Hargreaves K M; Papka R E
Description
An account of the resource
Before parturition the uterine cervix undergoes a ripening process ("softens" and dilates) to allow passage of the fetus at term. The exact mechanism(s) responsible for cervical ripening are unknown, though a role for peptidergic sensory neurons is emerging. Previous work demonstrated that administration of substance P (SP) to ovariectomized rats caused events associated with cervical ripening, that production of SP in cervix-related dorsal root ganglion (DRG) is estrogen responsive, and that release of SP from neurons terminating in the cervix and spinal cord peaks prior to parturition. The present study was designed to test the hypothesis that calcitonin gene-related peptide (CGRP), a neuropeptide co-stored with SP in many sensory neurons, undergoes changes with pregnancy and hormonal environment. Immunohistochemistry, in situ hybridization, reverse transcriptase-polymerase chain reaction (RT-PCR) and radioimmunoassay (RIA) were used to investigate CGRP in L6-S1 DRG, spinal cord and cervix during pregnancy and the role of estrogen in CGRP synthesis. CGRP-immunoreactive primary sensory neurons expressed estrogen receptors (ER-alpha and ER-beta). In the cervix, CGRP concentrations decreased, but in the L6-S1 DRG and the spinal cord segments, CGRP levels increased, with peak effects observed at day 20 of gestation. CGRP mRNA synthesis increased in DRG over pregnancy. Sensory neurons of ovariectomized rats treated with estrogen showed increased CGRP mRNA synthesis in a dose-related manner, an effect blocked by the ER antagonist ICI 182 780. From these results, we postulate that synthesis of CGRP in L6-S1 DRG and utilization in the cervix increase over pregnancy and this synthesis is the under influence of the estrogen-ER system. Collectively, these data are consistent with the hypothesis that CGRP plays a role in cervical ripening and, consequently in the birth process.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1016/j.peptides.2003.07.009" target="_blank" rel="noreferrer noopener">10.1016/j.peptides.2003.07.009</a>
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
2003
Animals
Calcitonin Gene-Related Peptide/analysis/biosynthesis/*genetics
Cervix Uteri/*physiology
Collins J
Department of Anatomy & Neurobiology
Estrogens/*physiology
Female
Ganglia
Hargreaves K M
Immunohistochemistry
In Situ Hybridization
Messenger/metabolism
Mowa C N
NEOMED College of Medicine
Papka R E
Peptides
Pregnancy
Radioimmunoassay
Rats
RNA
Spinal Cord/*physiology
Spinal/*physiology
Sprague-Dawley
Storey-Workley M
Usip S
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1016/j.nut.2016.08.004" target="_blank" rel="noreferrer noopener">http://doi.org/10.1016/j.nut.2016.08.004</a>
Pages
1–13
Volume
33
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Inhibition of cartilage degradation and suppression of PGE2 and MMPs expression by pomegranate fruit extract in a model of posttraumatic osteoarthritis.
Publisher
An entity responsible for making the resource available
Nutrition (Burbank, Los Angeles County, Calif.)
Date
A point or period of time associated with an event in the lifecycle of the resource
2017
2017-01
Subject
The topic of the resource
*Phytotherapy; *Punicaceae; ACLT; Animal; Animals; Anterior Cruciate Ligament/drug effects/metabolism/pathology; Apoptosis; Cartilage/cytology/*drug effects/metabolism/pathology; Chondrocytes/drug effects/metabolism/pathology; Collagen Type II/genetics/metabolism; Dinoprostone/*metabolism; Disease Models; Disease Progression; Female; Fruit; Interleukins/metabolism; Joints/cytology/*drug effects/metabolism/pathology; Male; Messenger/metabolism; Metalloproteases/genetics/*metabolism; Mitogen-Activated Protein Kinases/metabolism; MMPs; NF-kappa B/metabolism; Osteoarthritis; Osteoarthritis/*drug therapy/etiology/metabolism/pathology; PGE(2); Plant Extracts/pharmacology/therapeutic use; Pomegranate; Rabbit; Rabbits; RNA; Synovial Fluid/metabolism
Creator
An entity primarily responsible for making the resource
Akhtar Nahid; Khan Nazir M; Ashruf Omer S; Haqqi Tariq M
Description
An account of the resource
OBJECTIVE: Osteoarthritis (OA) is characterized by cartilage degradation in the affected joints. Pomegranate fruit extract (PFE) inhibits cartilage degradation in vitro. The aim of this study was to determine whether oral consumption of PFE inhibits disease progression in rabbits with surgically induced OA. METHODS: OA was surgically induced in the tibiofemoral joints of adult New Zealand White rabbits. In one group, animals were fed PFE in water for 8 wk postsurgery. In the second group, animals were fed PFE for 2 wk before surgery and for 8 wk postsurgery. Histologic assessment and scoring of the cartilage was per Osteoarthritis Research Society International guidelines. Gene expression and matrix metalloproteinases (MMP) activity were determined using quantitative reverse transcriptase polymerase chain reaction and fluorometric assay, respectively. Interleukin (IL)-1 beta, MMP-13, IL-6, prostaglandin (PG)E2, and type II collagen (COL2A1) levels in synovial fluid/plasma/culture media were quantified using enzyme-linked immunosorbent assay. Expression of active caspase-3 and poly (ADP-ribose) polymerase p85 was determined by immunohistochemistry. Effect of PFE and inhibitors of MMP-13, mitogen-activated protein kinase (MAPK) and nuclear factor (NF)-kappaB was studied in IL-1 beta-stimulated rabbit articular chondrocytes. RESULTS: Safranin-O-staining and chondrocyte cluster formation was significantly reduced in the anterior cruciate ligament transaction plus PFE fed groups. Expression of MMP-3, MMP-9, and MMP-13 mRNA was higher in the cartilage of rabbits given water alone but was significantly lower in the animals fed PFE. PFE-fed rabbits had lower IL-6,
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1016/j.nut.2016.08.004" target="_blank" rel="noreferrer noopener">10.1016/j.nut.2016.08.004</a>
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
*Phytotherapy
*Punicaceae
2017
ACLT
Akhtar Nahid
Animal
Animals
Anterior Cruciate Ligament/drug effects/metabolism/pathology
Apoptosis
Ashruf Omer S
Cartilage/cytology/*drug effects/metabolism/pathology
Chondrocytes/drug effects/metabolism/pathology
Collagen Type II/genetics/metabolism
Department of Anatomy & Neurobiology
Dinoprostone/*metabolism
Disease Models
Disease Progression
Female
Fruit
Haqqi Tariq M
Interleukins/metabolism
Joints/cytology/*drug effects/metabolism/pathology
Khan Nazir M
Male
Messenger/metabolism
Metalloproteases/genetics/*metabolism
Mitogen-Activated Protein Kinases/metabolism
MMPs
NEOMED College of Medicine
NF-kappa B/metabolism
Nutrition (Burbank, Los Angeles County, Calif.)
Osteoarthritis
Osteoarthritis/*drug therapy/etiology/metabolism/pathology
PGE(2)
Plant Extracts/pharmacology/therapeutic use
Pomegranate
Rabbit
Rabbits
RNA
Synovial Fluid/metabolism
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1016/j.neuropharm.2017.07.020" target="_blank" rel="noreferrer noopener">http://doi.org/10.1016/j.neuropharm.2017.07.020</a>
Pages
189–196
Volume
125
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Valproate increases dopamine transporter expression through histone acetylation and enhanced promoter binding of Nurr1.
Publisher
An entity responsible for making the resource available
Neuropharmacology
Date
A point or period of time associated with an event in the lifecycle of the resource
2017
2017-10
Subject
The topic of the resource
Acetylation/drug effects; Animals; Butyrates/pharmacology; Cell Line; Cell Survival/drug effects; Dopamine Plasma Membrane Transport Proteins/*metabolism; Dopamine transporter; Dopaminergic Neurons/cytology/drug effects/metabolism; Dose-Response Relationship; Drug; Epigenesis; Epigenetics; Genetic; Genetic/drug effects; Group A; HDAC; Histone deacetylase; Histone Deacetylase Inhibitors/*pharmacology; Histone Deacetylases/metabolism; Histones/*drug effects/metabolism; Homeodomain Proteins/metabolism; Hydroxamic Acids/pharmacology; Member 2/*metabolism; Messenger/metabolism; Nuclear Receptor Subfamily 4; Nurr1; Pitx3; Promoter Regions; Rats; RNA; Transcription Factors/metabolism; Valproate; Valproic Acid/*pharmacology
Creator
An entity primarily responsible for making the resource
Green Ashley L; Zhan Le; Eid Aseel; Zarbl Helmut; Guo Grace L; Richardson Jason R
Description
An account of the resource
The dopamine transporter (DAT) is the key regulator of dopaminergic transmission and is a target of several xenobiotics, including pesticides and pharmacological agents. Previously, we identified a prominent role for histone deacetylases in the regulation of DAT expression. Here, we utilized a rat dopaminergic cell line (N27) to probe the responsiveness of DAT mRNA expression to inhibitors of histone acetylation. Inhibition of histone deacetylases (HDACs) by valproate, butyrate and Trichostatin A led to a 3-10-fold increase in DAT mRNA expression, a 50% increase in protein levels, which were accompanied by increased H3 acetylation levels. To confirm the mechanism of valproate-mediated increase in DAT mRNA, chromatin immunoprecipitation (ChIP) assays were used and demonstrated a significant increase in enrichment of acetylation of histone 3 on lysines 9 and 14 (H3K9/K14ac) in the DAT promoter. Expression of Nurr1 and Pitx3, key regulators of DAT expression, were increased following valproate treatment and Nurr1 binding was enriched in the DAT promoter. Together, these results indicate that histone acetylation and subsequent enhancement of transcription factor binding are plausible mechanisms for DAT regulation by valproate and, perhaps, by other xenobiotics.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1016/j.neuropharm.2017.07.020" target="_blank" rel="noreferrer noopener">10.1016/j.neuropharm.2017.07.020</a>
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
2017
Acetylation/drug effects
Animals
Butyrates/pharmacology
Cell Line
Cell Survival/drug effects
Department of Pharmaceutical Sciences
Dopamine Plasma Membrane Transport Proteins/*metabolism
Dopamine transporter
Dopaminergic Neurons/cytology/drug effects/metabolism
Dose-Response Relationship
Drug
Eid Aseel
Epigenesis
Epigenetics
Genetic
Genetic/drug effects
Green Ashley L
Group A
Guo Grace L
HDAC
Histone deacetylase
Histone Deacetylase Inhibitors/*pharmacology
Histone Deacetylases/metabolism
Histones/*drug effects/metabolism
Homeodomain Proteins/metabolism
Hydroxamic Acids/pharmacology
Member 2/*metabolism
Messenger/metabolism
NEOMED College of Pharmacy
Neuropharmacology
Nuclear Receptor Subfamily 4
Nurr1
Pitx3
Promoter Regions
Rats
Richardson Jason R
RNA
Transcription Factors/metabolism
Valproate
Valproic Acid/*pharmacology
Zarbl Helmut
Zhan Le
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1016/j.neuro.2016.04.002" target="_blank" rel="noreferrer noopener">http://doi.org/10.1016/j.neuro.2016.04.002</a>
Pages
274–279
Volume
60
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Developmental pyrethroid exposure causes long-term decreases of neuronal sodium channel expression.
Publisher
An entity responsible for making the resource available
Neurotoxicology
Date
A point or period of time associated with an event in the lifecycle of the resource
2017
2017-05
Subject
The topic of the resource
Animals; BDNF; Brain-Derived Neurotrophic Factor/metabolism; Cerebral Cortex/*drug effects/growth & development/metabolism; Corpus Striatum/*drug effects/growth & development/metabolism; Deltamethrin; Female; Inbred C57BL; Insecticides/*toxicity; Male; Messenger/metabolism; Mice; Neurodevelopmental; Neurons/*drug effects/metabolism; Nitriles/*toxicity; Pregnancy; Prenatal Exposure Delayed Effects/*metabolism; Pyrethrins/*toxicity; Pyrethroid; RNA; Sodium channel; Voltage-Gated Sodium Channels/*metabolism
Creator
An entity primarily responsible for making the resource
Magby Jason P; Richardson Jason R
Description
An account of the resource
Pyrethroid insecticide use has increased over recent years because of their low to moderate acute toxicity in mammals. However, there is increasing concern over the potential detrimental effects of pyrethroids on developing animals. Most recently, we have shown that developmental exposure to deltamethrin results in long-term neurobehavioral effects. Pyrethroids exert their toxicity by acting on the voltage-gated sodium channel (Nav), delaying channel inactivation and causing hyperexcitability in the nervous system. Previous in vitro studies found that exposure to agents that increase Na(+) influx, including deltamethrin decreased Nav mRNA expression. However, it is unknown whether this occurs in vivo. To determine whether developmental pyrethroid exposure decreases Nav mRNA expression, pregnant mice were exposed to the pyrethroid deltamethrin (0 or 3mg/kg) every three days throughout gestation and lactation. Nav mRNA expression was measured in the striatum and cortex of the offspring at 10-11 months of age, a time at which behavioral abnormalities were still observed. Developmental exposure to deltamethrin decreased expression of Nav mRNA in a region- and isoform-specific fashion by 24-50%. Deltamethrin exposure also resulted in the persistent down-regulation of brain-derived neurotrophic factor (Bdnf) in the striatum by 66% but not in the cortex, suggesting a plausible mechanism for some of the associated behavioral effects observed previously. Taken together these data suggest that developmental deltamethrin exposure results in persistent deficits in Nav and BDNF mRNA expression that may contribute to long-term behavioral deficits.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1016/j.neuro.2016.04.002" target="_blank" rel="noreferrer noopener">10.1016/j.neuro.2016.04.002</a>
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Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
2017
Animals
BDNF
Brain-Derived Neurotrophic Factor/metabolism
Cerebral Cortex/*drug effects/growth & development/metabolism
Corpus Striatum/*drug effects/growth & development/metabolism
Deltamethrin
Department of Pharmaceutical Sciences
Female
Inbred C57BL
Insecticides/*toxicity
Magby Jason P
Male
Messenger/metabolism
Mice
NEOMED College of Pharmacy
Neurodevelopmental
Neurons/*drug effects/metabolism
Neurotoxicology
Nitriles/*toxicity
Pregnancy
Prenatal Exposure Delayed Effects/*metabolism
Pyrethrins/*toxicity
Pyrethroid
Richardson Jason R
RNA
Sodium channel
Voltage-Gated Sodium Channels/*metabolism
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1016/j.nbd.2017.08.009" target="_blank" rel="noreferrer noopener">http://doi.org/10.1016/j.nbd.2017.08.009</a>
Pages
115–127
Volume
108
Dublin Core
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Title
A name given to the resource
Alternative microglial activation is associated with cessation of progressive dopamine neuron loss in mice systemically administered lipopolysaccharide.
Publisher
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Neurobiology of disease
Date
A point or period of time associated with an event in the lifecycle of the resource
2017
2017-12
Subject
The topic of the resource
Animals; Astrocytes/immunology/pathology; Cell Death/physiology; Corpus Striatum/immunology/pathology; Cytokines/metabolism; Disease Progression; Dopaminergic Neurons/*immunology/pathology; Inbred C57BL; Inflammation/pathology/physiopathology; Lipopolysaccharides/*toxicity; Male; Messenger/metabolism; Mice; Microglia/*immunology/pathology; Nerve Degeneration/*immunology/pathology; Neurodegenerative Diseases/immunology/pathology; Neuroimmunomodulation/physiology; Random Allocation; RNA; Time Factors
Creator
An entity primarily responsible for making the resource
Beier Eric E; Neal Matthew; Alam Gelerah; Edler Melissa; Wu Long-Jun; Richardson Jason R
Description
An account of the resource
Inflammation arising from central and/or peripheral sources contributes to the pathogenesis of multiple neurodegenerative diseases including Parkinson's disease (PD). Emerging data suggest that differential activation of glia could lead to the pathogenesis and progression of PD. Here, we sought to determine the relationship between lipopolysaccharide (LPS) treatment, loss of dopaminergic neurons and differential activation of glia. Using a model of repeated injections with LPS (1mg/kg, i.p. for 4days), we found that LPS induced a 34% loss of dopamine neurons in the substantia nigra 19days after initiation of treatment, but no further cell loss was observed at 36days. LPS induced a strong pro-inflammatory response with increased mRNA expression of pro-inflammatory markers, including tumor necrosis factor-alpha (4.8-fold), inducible nitric oxide synthase (2.0-fold), interleukin-1 beta (8.9-fold), interleukin-6 (10.7-fold), and robust glial activation were observed at 1day after final dose of LPS. These pro-inflammatory genes were then reduced at 19days after treatment, when there was a rise in the anti-inflammatory genes Ym1 (1.8-fold) and arginase-1 (2.6-fold). Additionally, 36days after the last LPS injection there was a significant increase in interleukin-10 (2.1-fold) expression. The qPCR data results were supported by protein data, including cytokine measurements, western blotting, and immunofluorescence in brain microglia. Taken together, these data demonstrate that progressive neurodegeneration in the substantia nigra following LPS is likely arrested by microglia shifting to an anti-inflammatory phenotype. Thus, strategies to promote resolution of neuroinflammation may be a promising avenue to slow the progressive loss of dopamine neurons in PD.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1016/j.nbd.2017.08.009" target="_blank" rel="noreferrer noopener">10.1016/j.nbd.2017.08.009</a>
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Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
2017
Alam Gelerah
Animals
Astrocytes/immunology/pathology
Beier Eric E
Cell Death/physiology
Corpus Striatum/immunology/pathology
Cytokines/metabolism
Department of Pharmaceutical Sciences
Disease Progression
Dopaminergic Neurons/*immunology/pathology
Edler Melissa
Inbred C57BL
Inflammation/pathology/physiopathology
Lipopolysaccharides/*toxicity
Male
Messenger/metabolism
Mice
Microglia/*immunology/pathology
Neal Matthew
NEOMED College of Pharmacy
Nerve Degeneration/*immunology/pathology
Neurobiology of disease
Neurodegenerative Diseases/immunology/pathology
Neuroimmunomodulation/physiology
Random Allocation
Richardson Jason R
RNA
Time Factors
Wu Long-Jun
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1016/0304-3940(95)12085-i" target="_blank" rel="noreferrer noopener">http://doi.org/10.1016/0304-3940(95)12085-i</a>
Pages
2–4
Issue
1
Volume
200
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Expression of alpha-cardiac myosin heavy chain in normal and denervated rat muscle spindles.
Publisher
An entity responsible for making the resource available
Neuroscience letters
Date
A point or period of time associated with an event in the lifecycle of the resource
1995
1995-11
Subject
The topic of the resource
Animals; Denervation; In Situ Hybridization; Messenger/metabolism; Muscle Spindles/*metabolism/physiology; Myocardium/*metabolism; Myosin Heavy Chains/*metabolism; Rats; RNA; Sprague-Dawley; Time Factors
Creator
An entity primarily responsible for making the resource
McWhorter D L; Walro J M; Signs S A; Wang J
Description
An account of the resource
Whether any fibers in rat hindlimb muscles express alpha-cardiac myosin heavy chain (MHC) is uncertain. Expression of alpha-cardiac MHC mRNA and the polypeptide for which it codes were examined in control and denervated rat muscle spindles using in situ hybridization (ISH) and immunocytochemistry (ICC). Both nuclear bag2 and bag1 intrafusal fibers in the extensor digitorum longus (EDL) muscles expressed alpha-cardiac MHC and its precursor mRNA. Furthermore, denervation of the hindlimb down-regulated alpha-cardiac MHC mRNA expression in rat nuclear bag intrafusal fibers, even though they continued to display a strong affinity for anti-alpha-cardiac MHC monoclonal antibody. These data show that (1) intrafusal fibers express the alpha-cardiac MHC gene; (2) innervation regulates alpha-cardiac MHC gene expression at a pre-translational level; and (3) ISH is more sensitive than ICC to changes in alpha-cardiac MHC gene expression in adult rat muscle spindles.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1016/0304-3940(95)12085-i" target="_blank" rel="noreferrer noopener">10.1016/0304-3940(95)12085-i</a>
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Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
1995
Animals
Denervation
In Situ Hybridization
McWhorter D L
Messenger/metabolism
Muscle Spindles/*metabolism/physiology
Myocardium/*metabolism
Myosin Heavy Chains/*metabolism
Neuroscience letters
Rats
RNA
Signs S A
Sprague-Dawley
Time Factors
Walro J M
Wang J
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1007/bf00202825" target="_blank" rel="noreferrer noopener">http://doi.org/10.1007/bf00202825</a>
Pages
568–570
Issue
5
Volume
93
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
A splicing mutation in the cytochrome b5 gene from a patient with congenital methemoglobinemia and pseudohermaphrodism.
Publisher
An entity responsible for making the resource available
Human genetics
Date
A point or period of time associated with an event in the lifecycle of the resource
1994
1994-05
Subject
The topic of the resource
*DNA; *Mutation; Base Sequence; Cytochromes b5/*genetics; Disorders of Sex Development/*enzymology/genetics; DNA Primers/chemistry; Gene Deletion; Humans; Leukocytes/metabolism; Male; Messenger/metabolism; Methemoglobinemia/*congenital/*enzymology; Molecular Sequence Data; Polymerase Chain Reaction; Recombinant; Reticulocytes/metabolism; RNA
Creator
An entity primarily responsible for making the resource
Giordano S J; Kaftory A; Steggles A W
Description
An account of the resource
We have analyzed reticulocyte and leukocyte mRNAs isolated from a patient with congenital methemoglobinemia and pseudohermaphrodism. The cytochrome b5 cDNA sequences were amplified using specific oligonucleotide primers and the polymerase chain reaction (PCR). DNA sequencing indicated that there was a 16-bp deletion in the cDNA leading to a new, in-frame stop signal and resulting in a truncated protein of 45 amino acids. Genomic DNA was analyzed, and the molecular lesion was shown to be an AG–\textgreaterGG alteration in the 3' splicing junction of intron 1. The splice site alteration leads to the usage of the nearest AG as an alternative splice site, resulting in a 16-bp deletion in the mRNA. All of the studies on reticulocyte mRNA and genomic DNA indicated that the patient was homozygous for the lesion.
Identifier
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<a href="http://doi.org/10.1007/bf00202825" target="_blank" rel="noreferrer noopener">10.1007/bf00202825</a>
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
*DNA
*Mutation
1994
Base Sequence
Cytochromes b5/*genetics
Disorders of Sex Development/*enzymology/genetics
DNA Primers/chemistry
Gene Deletion
Giordano S J
Human genetics
Humans
Kaftory A
Leukocytes/metabolism
Male
Messenger/metabolism
Methemoglobinemia/*congenital/*enzymology
Molecular Sequence Data
Polymerase Chain Reaction
Recombinant
Reticulocytes/metabolism
RNA
Steggles A W
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1002/jcp.25279" target="_blank" rel="noreferrer noopener">http://doi.org/10.1002/jcp.25279</a>
Pages
1761–1770
Issue
8
Volume
231
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Osteoactivin Promotes Migration of Oral Squamous Cell Carcinomas.
Publisher
An entity responsible for making the resource available
Journal of cellular physiology
Date
A point or period of time associated with an event in the lifecycle of the resource
2016
2016-08
Subject
The topic of the resource
*Cell Movement; Carcinoma; Cell Adhesion; Cell Line; Cell Proliferation; Cell Survival; Enzyme Activation; Gene Expression Regulation; Head and Neck Neoplasms/genetics/*metabolism/pathology; Humans; Integrin beta1/metabolism; Membrane Glycoproteins/genetics/*metabolism; Messenger/metabolism; Mitogen-Activated Protein Kinases/metabolism; Mouth Neoplasms/genetics/*metabolism/pathology; Neoplasm Invasiveness; Neoplastic; Protein Binding; RNA; RNA Interference; Signal Transduction; Squamous Cell Carcinoma of Head and Neck; Squamous Cell/genetics/*metabolism/pathology; Time Factors; Transfection; Tumor
Creator
An entity primarily responsible for making the resource
Arosarena Oneida A; Dela Cadena Raul A; Denny Michael F; Bryant Evan; Barr Eric W; Thorpe Ryan; Safadi Fayez F
Description
An account of the resource
Nearly 50% of patients with oral squamous cell carcinoma (OSCC) die of metastases or locoregional recurrence. Metastasis is mediated by cancer cell adhesion, migration, and invasion. Osteoactivin (OA) overexpression plays a role in metastases in several malignancies. The aims were to determine how integrin interactions modulate OA-induced OSCC cell migration; and to investigate OA effects on cell survival and proliferation. We confirmed OA mRNA and protein overexpression in OSCC cell lines. We assessed OA's interactions with integrins using adhesion inhibition assays, fluorescent immunocytochemistry and co-immunoprecipitation. We investigated OA-mediated activation of mitogen-activated protein kinases (MAPKs) and cell survival. Integrin inhibition effects on OA-mediated cell migration were determined. We assessed effects of OA knock-down on cell migration and proliferation. OA is overexpressed in OSCC cell lines, and serves as a migration-promoting adhesion molecule. OA co-localized with integrin subunits, and co-immunoprecipitated with the subunits. Integrin blocking antibodies, especially those directed against the beta1 subunit, inhibited cell adhesion (P = 0.03 for SCC15 cells). Adhesion to OA activated MAPKs in UMSCC14a cells and OA treatment promoted survival of SCC15 cells. Integrin-neutralizing antibodies enhanced cell migration with OA in the extracellular matrix. OA knock-down resulted in decreased proliferation of SCC15 and SCC25 cells, but did not inhibit cell migration. OA in the extracellular matrix promotes OSCC cell adhesion and migration, and may be a novel target in the prevention of HNSCC spread. J. Cell. Physiol. 231: 1761-1770, 2016. (c) 2015 Wiley Periodicals, Inc.
Identifier
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<a href="http://doi.org/10.1002/jcp.25279" target="_blank" rel="noreferrer noopener">10.1002/jcp.25279</a>
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
*Cell Movement
2016
Arosarena Oneida A
Barr Eric W
Bryant Evan
Carcinoma
Cell Adhesion
Cell Line
Cell Proliferation
Cell Survival
Dela Cadena Raul A
Denny Michael F
Department of Anatomy & Neurobiology
Enzyme Activation
Gene Expression Regulation
Head and Neck Neoplasms/genetics/*metabolism/pathology
Humans
Integrin beta1/metabolism
Journal of cellular physiology
Membrane Glycoproteins/genetics/*metabolism
Messenger/metabolism
Mitogen-Activated Protein Kinases/metabolism
Mouth Neoplasms/genetics/*metabolism/pathology
NEOMED College of Medicine
Neoplasm Invasiveness
Neoplastic
Protein Binding
RNA
RNA Interference
Safadi Fayez F
Signal Transduction
Squamous Cell Carcinoma of Head and Neck
Squamous Cell/genetics/*metabolism/pathology
Thorpe Ryan
Time Factors
Transfection
Tumor
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1002/hep.26427" target="_blank" rel="noreferrer noopener">http://doi.org/10.1002/hep.26427</a>
Pages
1111–1121
Issue
3
Volume
58
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Regulation of cholesterol and bile acid homeostasis by the cholesterol 7alpha-hydroxylase/steroid response element-binding protein 2/microRNA-33a axis in mice.
Publisher
An entity responsible for making the resource available
Hepatology (Baltimore, Md.)
Date
A point or period of time associated with an event in the lifecycle of the resource
2013
2013-09
Subject
The topic of the resource
Acetyl Coenzyme A/metabolism; Animal; Animals; Bile Acids and Salts/*metabolism; Cholesterol 7-alpha-Hydroxylase/genetics/*metabolism; Cholesterol/*metabolism; Homeostasis/*physiology; Knockout; Lipid Metabolism/physiology; Liver/metabolism; Male; Messenger/metabolism; Mice; MicroRNAs/*metabolism; Models; RNA; Signal Transduction/*physiology; Sterol Regulatory Element Binding Protein 2/*metabolism; Transgenic
Creator
An entity primarily responsible for making the resource
Li Tiangang; Francl Jessica M; Boehme Shannon; Chiang John Y L
Description
An account of the resource
UNLABELLED: Bile acid synthesis not only produces physiological detergents required for intestinal nutrient absorption, but also plays a critical role in regulating hepatic and whole-body metabolic homeostasis. We recently reported that overexpression of cholesterol 7alpha-hydroxylase (CYP7A1) in the liver resulted in improved metabolic homeostasis in Cyp7a1 transgenic (Cyp7a1-tg) mice. This study further investigated the molecular links between bile acid metabolism and lipid homeostasis. Microarray gene profiling revealed that CYP7A1 overexpression led to marked activation of the steroid response element-binding protein 2 (SREBP2)-regulated cholesterol metabolic network and absence of bile acid repression of lipogenic gene expression in livers of Cyp7a1-tg mice. Interestingly, Cyp7a1-tg mice showed significantly elevated hepatic cholesterol synthesis rates, but reduced hepatic fatty acid synthesis rates, which was accompanied by increased (14) C-glucose-derived acetyl-coenzyme A incorporation into sterols for fecal excretion. Induction of SREBP2 also coinduces intronic microRNA-33a (miR-33a) in the SREBP2 gene in Cyp7a1-tg mice. Overexpression of miR-33a in the liver resulted in decreased bile acid pool, increased hepatic cholesterol content, and lowered serum cholesterol in mice. CONCLUSION: This study suggests that a CYP7A1/SREBP2/miR-33a axis plays a critical role in regulation of hepatic cholesterol, bile acid, and fatty acid synthesis. Antagonism of miR-33a may be a potential strategy to increase bile acid synthesis to maintain lipid homeostasis and prevent nonalcoholic fatty liver disease, diabetes, and obesity.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1002/hep.26427" target="_blank" rel="noreferrer noopener">10.1002/hep.26427</a>
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
2013
Acetyl Coenzyme A/metabolism
Animal
Animals
Bile Acids and Salts/*metabolism
Boehme Shannon
Chiang John Y L
Cholesterol 7-alpha-Hydroxylase/genetics/*metabolism
Cholesterol/*metabolism
Department of Integrative Medical Sciences
Francl Jessica M
Hepatology (Baltimore, Md.)
Homeostasis/*physiology
Knockout
Li Tiangang
Lipid Metabolism/physiology
Liver/metabolism
Male
Messenger/metabolism
Mice
MicroRNAs/*metabolism
Models
NEOMED College of Medicine
RNA
Signal Transduction/*physiology
Sterol Regulatory Element Binding Protein 2/*metabolism
Transgenic