1
40
3
-
Text
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URL Address
<a href="http://doi.org/10.1080/03008207.2016.1211113" target="_blank" rel="noreferrer noopener">http://doi.org/10.1080/03008207.2016.1211113</a>
Pages
64–75
Issue
1
Volume
58
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Histone deacetylase inhibitor vorinostat (SAHA, MK0683) perturb miR-9-MCPIP1 axis to block IL-1beta-induced IL-6 expression in human OA chondrocytes.
Publisher
An entity responsible for making the resource available
Connective tissue research
Date
A point or period of time associated with an event in the lifecycle of the resource
2017
2017-01
Subject
The topic of the resource
*IL-6; *MCPIP1; *miRNA; *Osteoarthritis; *Vorinostat; Adult; Aged; CCAAT-Enhancer-Binding Proteins/genetics/metabolism; Cells; Chondrocytes/*metabolism/pathology; Cultured; Female; Gene Expression Regulation/*drug effects; Genetic; Humans; Hydroxamic Acids/*pharmacology; Interleukin-1beta/genetics/*metabolism; Interleukin-6/genetics/*metabolism; Male; MicroRNAs/genetics/*metabolism; Middle Aged; Osteoarthritis/drug therapy/genetics/*metabolism/pathology; Promoter Regions; Ribonucleases/*biosynthesis/genetics; Transcription Factors/*biosynthesis/genetics; Vorinostat
Creator
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Makki Mohammad S; Haqqi Tariq M
Description
An account of the resource
AIM OF THE STUDY: High levels of IL-6 are believed to contribute to osteoarthritis (OA) pathogenesis. The expression of IL-6 is regulated post-transcriptionally by the miR-9-MCPIP-1 axis in chondrocytes. Vorinostat (SAHA) inhibits the IL-6 expression in OA chondrocytes. We investigated whether SAHA suppresses the expression of IL-6 by perturbing the miR-9-MCPIP1 axis in OA chondrocytes under pathological conditions. MATERIALS AND METHODS: OA chondrocytes were isolated by enzymatic digestion and treated with IL-1beta in the absence or presence of SAHA. Genes and protein expression levels were determined by TaqMan assays and Western blotting, respectively. Secreted IL-6 was quantified by enzyme linked immunosorbent assay (ELISA). MCPIP1 promoter deletion mutants were generated by polymerase chain reaction (PCR). Promoter recruitment of transcription factors was determined by ChIP. Nuclear run-on was employed to measure the ongoing transcription. siRNA-mediated knockdown of the CEBPalpha expression was employed for loss of function studies. RESULTS: Expression of MCPIP1 was high in SAHA treated OA chondrocytes but expression of IL-6 mRNAs and secreted IL-6 were reduced by \textasciitilde70%. SAHA suppressed the expression of miR-9 but enhanced the activity of the MCPIP1 promoter localized to a 156bp region which also harbors the binding site for CEBPalpha. Treatment with SAHA enhanced the recruitment of CEBPalpha to the MCPIP1 promoter. Ectopically expressed CEBPalpha enhanced the promoter activity and the expression of MCPIP1 while siRNA-mediated knockdown of CEBPalpha inhibited the expression of MCPIP1. CONCLUSIONS: Taken together our data indicate that SAHA-mediated suppression of the IL-6 expression is achieved through increased recruitment of CEBPalpha to the MCPIP1 promoter and by relieving the miR-9-mediated inhibition of MCPIP1 expression in OA chondrocytes.
Identifier
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<a href="http://doi.org/10.1080/03008207.2016.1211113" target="_blank" rel="noreferrer noopener">10.1080/03008207.2016.1211113</a>
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Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
*IL-6
*MCPIP1
*miRNA
*OSTEOARTHRITIS
*Vorinostat
2017
Adult
Aged
CCAAT-Enhancer-Binding Proteins/genetics/metabolism
Cells
Chondrocytes/*metabolism/pathology
Connective tissue research
Cultured
Department of Anatomy & Neurobiology
Female
Gene Expression Regulation/*drug effects
Genetic
Haqqi Tariq M
Humans
Hydroxamic Acids/*pharmacology
Interleukin-1beta/genetics/*metabolism
Interleukin-6/genetics/*metabolism
Makki Mohammad S
Male
MicroRNAs/genetics/*metabolism
Middle Aged
NEOMED College of Medicine
Osteoarthritis/drug therapy/genetics/*metabolism/pathology
Promoter Regions
Ribonucleases/*biosynthesis/genetics
Transcription Factors/*biosynthesis/genetics
Vorinostat
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1007/s11926-016-0604-x" target="_blank" rel="noreferrer noopener">http://doi.org/10.1007/s11926-016-0604-x</a>
Pages
56–56
Issue
8
Volume
18
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
The Role of MicroRNAs and Their Targets in Osteoarthritis.
Publisher
An entity responsible for making the resource available
Current rheumatology reports
Date
A point or period of time associated with an event in the lifecycle of the resource
2016
2016-08
Subject
The topic of the resource
*Cartilage; *Inflammation; *MicroRNA; *Osteoarthritis; *Targets; Articular/*metabolism; Cartilage; Inflammation/genetics/*metabolism; Intracellular Signaling Peptides and Proteins; MicroRNAs/genetics/*metabolism; Osteoarthritis/genetics/*metabolism
Creator
An entity primarily responsible for making the resource
Sondag Gregory R; Haqqi Tariq M
Description
An account of the resource
Micro ribonucleic acid (microRNA) regulation and expression has become an emerging field in determining the mechanisms regulating a variety of inflammation-mediated diseases. Several studies have focused on specific microRNAs that are differentially expressed in cases of osteoarthritis. Furthermore, several targets of these miRNAs important in disease progression have also been identified. In this review, we focus on microRNA biogenesis, regulation, detection, and quantification with an emphasis on cellular localization and how these concepts may be linked to disease processes such as osteoarthritis. Next, we review the relationships of specific microRNAs to certain features and risk factors associated with osteoarthritis such as inflammation, obesity, autophagy, and cartilage homeostasis. We also identify certain microRNAs that are differentially expressed in osteoarthritis but have unidentified targets and functions in the disease state. Lastly, we identify the potential use of microRNAs for therapeutic purposes and also mention certain remedies that regulate microRNA expression.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1007/s11926-016-0604-x" target="_blank" rel="noreferrer noopener">10.1007/s11926-016-0604-x</a>
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Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
*Cartilage
*Inflammation
*MicroRNA
*OSTEOARTHRITIS
*Targets
2016
Articular/*metabolism
Cartilage
Current rheumatology reports
Department of Anatomy & Neurobiology
Haqqi Tariq M
Inflammation/genetics/*metabolism
Intracellular Signaling Peptides and Proteins
MicroRNAs/genetics/*metabolism
NEOMED College of Medicine
Osteoarthritis/genetics/*metabolism
Sondag Gregory R
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1002/art.38952" target="_blank" rel="noreferrer noopener">http://doi.org/10.1002/art.38952</a>
Pages
423–434
Issue
2
Volume
67
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
MicroRNA-602 and microRNA-608 regulate sonic hedgehog expression via target sites in the coding region in human chondrocytes.
Publisher
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Arthritis & rheumatology (Hoboken, N.J.)
Date
A point or period of time associated with an event in the lifecycle of the resource
2015
2015-02
Subject
The topic of the resource
Academic Medical Centers; Aged; Analysis of Variance; Animal; Animals; Anterior Cruciate Ligament/surgery; Blotting; Cells; Chondrocytes – Physiology; Chondrocytes/drug effects/*metabolism/pathology; Cultured; Disease Models; Female; Funding Source; Gene Expression Regulation/genetics/*physiology; Hedgehog Proteins/genetics/*metabolism; HEK293 Cells; Human; Humans; Immunohistochemistry; In Vitro Techniques; Interleukin-1beta/pharmacology; Knee/etiology/*metabolism/pathology; Male; Matrix Metalloproteinase 13/metabolism; MicroRNAs/genetics/*metabolism; Middle Age; Middle Aged; Ohio; Open Reading Frames/genetics/*physiology; Osteoarthritis; Osteoarthritis – Epidemiology; Osteoarthritis – Physiopathology; Polymerase Chain Reaction; Rabbits; Signal Transduction/genetics/physiology; T-Tests; Transfection; Up-Regulation/drug effects/genetics/physiology; Western
Creator
An entity primarily responsible for making the resource
Akhtar Nahid; Makki Mohammad S; Haqqi Tariq M
Description
An account of the resource
OBJECTIVE: Hedgehog (HH) signaling has recently been associated with cartilage degradation in osteoarthritis (OA). Because interleukin-1beta (IL-1beta) has been implicated as a principal instigator of OA, we sought to determine whether
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1002/art.38952" target="_blank" rel="noreferrer noopener">10.1002/art.38952</a>
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
2015
Academic Medical Centers
Aged
Akhtar Nahid
Analysis of Variance
Animal
Animals
Anterior Cruciate Ligament/surgery
Arthritis & rheumatology (Hoboken, N.J.)
Blotting
Cells
Chondrocytes – Physiology
Chondrocytes/drug effects/*metabolism/pathology
Cultured
Department of Anatomy & Neurobiology
Disease Models
Female
Funding Source
Gene Expression Regulation/genetics/*physiology
Haqqi Tariq M
Hedgehog Proteins/genetics/*metabolism
HEK293 Cells
Human
Humans
Immunohistochemistry
In Vitro Techniques
Interleukin-1beta/pharmacology
Knee/etiology/*metabolism/pathology
Makki Mohammad S
Male
Matrix Metalloproteinase 13/metabolism
MicroRNAs/genetics/*metabolism
Middle Age
Middle Aged
NEOMED College of Medicine
Ohio
Open Reading Frames/genetics/*physiology
Osteoarthritis
Osteoarthritis – Epidemiology
Osteoarthritis – Physiopathology
Polymerase Chain Reaction
Rabbits
Signal Transduction/genetics/physiology
T-Tests
Transfection
Up-Regulation/drug effects/genetics/physiology
Western