A novel bile acid-activated vitamin D receptor signaling in human hepatocytes.
Calcitriol/*metabolism; Calcitriol/pharmacology; Cell Membrane/drug effects/metabolism; Cell Nucleus/drug effects/metabolism; Cholesterol 7-alpha-Hydroxylase/antagonists & inhibitors/genetics; Enzyme Activation/drug effects; Extracellular Signal-Regulated MAP Kinases/antagonists & inhibitors; Genetic/genetics; Hep G2 Cells; Hepatocyte Nuclear Factor 4/metabolism; Hepatocytes/*drug effects/enzymology/*metabolism; Humans; Intracellular Space/drug effects/metabolism; Ligands; Lithocholic Acid/*pharmacology; Mitogen-Activated Protein Kinase Kinases/metabolism; Phosphorylation/drug effects; Phosphotyrosine/metabolism; Promoter Regions; Protein Kinase Inhibitors/pharmacology; Protein Transport/drug effects; Proto-Oncogene Proteins c-raf/metabolism; Receptors; Retinoid X Receptor alpha/metabolism; Signal Transduction/*drug effects; src-Family Kinases/metabolism; Steroid Hydroxylases/genetics/metabolism; Vitamin D3 24-Hydroxylase
Vitamin D receptor (VDR) is activated by natural ligands, 1alpha,
Han Shuxin; Li Tiangang; Ellis Ewa; Strom Stephen; Chiang John Y L
Molecular endocrinology (Baltimore, Md.)
2010
2010-06
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
<a href="http://doi.org/10.1210/me.2009-0482" target="_blank" rel="noreferrer noopener">10.1210/me.2009-0482</a>
Stimulation of MAP kinase pathways after maternal IL-1beta exposure induces fetal lung fluid absorption in guinea pigs.
Absorption/drug effects; Animal/*metabolism; Animals; Body Fluids/*metabolism; Extracellular Signal-Regulated MAP Kinases/metabolism; Female; Fetus/metabolism; Gestational Age; Guinea Pigs; Hydrocortisone/*metabolism; Injections; Interleukin-1beta/administration & dosage/*pharmacology; Lung/*embryology; Mitogen-Activated Protein Kinase Kinases/metabolism; Mitogen-Activated Protein Kinases/*metabolism; Pregnancy; Rats; Recombinant Proteins/administration & dosage/pharmacology; Subcutaneous
BACKGROUND: We tested the hypothesis that maternal interleukin-1beta (IL-1beta) pretreatment and induction of fetal cortisol synthesis activates MAP kinases and thereby affects lung fluid absorption in preterm guinea pigs. METHODS: IL-1beta was administered subcutaneously daily to timed-pregnant guinea pigs for three days. Fetuses were obtained by abdominal hysterotomy and instilled with isosmolar 5% albumin into the lungs and lung fluid movement was measured over 1 h by mass balance. MAP kinase expression was measured by western blot. RESULTS: Lung fluid absorption was induced at 61 days (D) gestation and stimulated at 68D gestation by IL-1beta. Maternal IL-1beta pretreatment upregulated ERK and upstream MEK expression at both 61 and 68D gestation, albeit being much more pronounced at 61D gestation. U0126 instillation completely blocked IL-1beta-induced lung fluid absorption as well as IL-1beta-induced/stimulated ERK expression. Cortisol synthesis inhibition by metyrapone attenuated ERK expression and lung fluid absorption in IL-1beta-pretreated fetal lungs. JNK expression after maternal
Bhattacharjee Reshma; Li Tianbo; Koshy Shyny; Beard LaMonta L; Sharma Kapil; Carter Ethan P; Garat Chrystelle; Folkesson Hans G
Respiratory research
2007
2007-03
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
<a href="http://doi.org/10.1186/1465-9921-8-27" target="_blank" rel="noreferrer noopener">10.1186/1465-9921-8-27</a>