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Text
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<a href="http://doi.org/10.1016/j.ymthe.2005.03.021" target="_blank" rel="noreferrer noopener">http://doi.org/10.1016/j.ymthe.2005.03.021</a>
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Pages
725-733
Issue
4
Volume
12
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Title
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Specific And Efficient Transduction Of Cochlear Inner Hair Cells With Recombinant Adeno-associated Virus Type 3 Vector
Publisher
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Molecular Therapy
Date
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2005
2005-10
Subject
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adeno-associated virus; adenovirus; Biotechnology & Applied Microbiology; cochlea; ear; Experimental Medicine; gene transfer; gene transfer; generation; Genetics & Heredity; guinea-pig cochlea; hair cells; in-vivo; promoter; rat; Research &; serotype; therapy; transgene expression
Creator
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Liu Y H; Okada T; Sheykholeslami K; Shimazaki K; Nomoto T; Muramatsu S I; Kanazawa T; Takeuchi K; Ajalli R; Mizukami H; Kume A; Ichimura K; Ozawa K
Description
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Recombinant adeno-associated virus (AAV) vectors are of interest for cochlear gene therapy because of their ability to mediate the efficient transfer and long-term stable expression of therapeutic genes in a wide variety of postmitotic tissues with minimal vector-related cytotoxicity. In the present study, seven AAV serotypes (AAV1-5, 7, 8) were used to construct vectors. The expression of EGFP by the chicken P-actin promoter associated with the cytomegalovirus immediate-early enhancer in cochlear cells showed that each of these serotypes successfully targets distinct cochlear cell types. In contrast to the other serotypes, the AAV3 vector specifically transduced cochlear inner hair cells with high efficiency in vivo, while the AAV1, 2, 5, 7, and 8 vectors also transduced these and other cell types, including spiral ganglion and spiral ligament cells. There was no loss of cochlear function with respect to evoked auditory brain-stem responses over the range of frequencies tested after the injection of AAV vectors. These findings are of value for further molecular studies of cochlear inner hair cells and for gene replacement strategies to correct recessive genetic hearing loss due to monogenic mutations in these cells.
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<a href="http://doi.org/10.1016/j.ymthe.2005.03.021" target="_blank" rel="noreferrer noopener">10.1016/j.ymthe.2005.03.021</a>
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Journal Article or Conference Abstract Publication
2005
adeno-associated virus
adenovirus
Ajalli R
Biotechnology & Applied Microbiology
Cochlea
Ear
Experimental Medicine
gene transfer
generation
Genetics & Heredity
guinea-pig cochlea
Hair Cells
Ichimura K
in-vivo
Journal Article or Conference Abstract Publication
Kanazawa T
Kume A
Liu Y H
Mizukami H
Molecular Therapy
Muramatsu S I
Nomoto T
Okada T
Ozawa K
promoter
rat
Research &
serotype
Sheykholeslami K
Shimazaki K
Takeuchi K
therapy
transgene expression