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URL Address
<a href="http://doi.org/10.1002/ptr.5799" target="_blank" rel="noreferrer noopener">http://doi.org/10.1002/ptr.5799</a>
Pages
778–782
Issue
5
Volume
31
Dublin Core
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Title
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A Polyphenol-rich Pomegranate Fruit Extract Suppresses NF-kappaB and IL-6 Expression by Blocking the Activation of IKKbeta and NIK in Primary Human Chondrocytes.
Publisher
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Phytotherapy research : PTR
Date
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2017
2017-05
Subject
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chondrocytes; Chondrocytes – Drug Effects; Chondrocytes/drug effects; Fruit; Fruit/chemistry; Gene Expression Regulation/drug effects; Genes – Drug Effects; Humans; I-kappa B Kinase/genetics/*metabolism; IkappaB; IKKbeta; IL-1beta; Interleukin 1 – Metabolism; Interleukin-1beta/metabolism; Interleukin-6/genetics/*metabolism; Interleukins; Interleukins – Metabolism; NF-kappa B; NF-kappa B – Metabolism; NF-kappa B/genetics/*metabolism; NF-kappaB; NIK; osteoarthritis; Phosphorylation – Drug Effects; Phosphorylation/drug effects; Plant Extracts; Plant Extracts – Pharmacodynamics; Plant Extracts/chemistry/*pharmacology; Polyphenols – Pharmacodynamics; Polyphenols/pharmacology; pomegranate; Pomegranate; Protein-Serine-Threonine Kinases/genetics/*metabolism; Proteins – Metabolism; Punicaceae/*chemistry; Signal Transduction – Drug Effects; Signal Transduction/drug effects; Transcription Factor RelA/metabolism; Transferases; Transferases – Metabolism
Creator
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Haseeb Abdul; Khan Nazir M; Ashruf Omer S; Haqqi Tariq M
Description
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Pomegranate fruit extract (PE) rich in polyphenols has been shown to exert chondroprotective effects, but the mechanism is not established. Here, we used an in vitro model of inflammation in osteoarthritis (OA) to investigate the potential of PE to suppress interleukin 1 beta (IL-1beta)-stimulated expression of inflammatory cytokine IL-6, generation of reactive oxygen species (ROS) levels, and investigated the mechanism of NF-kappaB inhibition by analyzing the activation of the kinases upstream of IkappaBalpha in primary human chondrocytes. Total and phosphorylated forms of kinases and expression of IL-6 were determined at protein and mRNA levels by western immunoblotting and Taqman assay, respectively. Dihydrorhodamine 123 staining estimated ROS generation. Pomegranate fruit extract inhibited the mRNA and protein expression of IL-6, generation of ROS, and inhibited the IL-1beta-mediated phosphorylation of inhibitor of nuclear factor kappa-B kinase subunit beta (IKKbeta), expression of IKKbeta mRNA, degradation of IkappaBalpha, and activation and nuclear translocation of
Identifier
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<a href="http://doi.org/10.1002/ptr.5799" target="_blank" rel="noreferrer noopener">10.1002/ptr.5799</a>
Rights
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Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
2017
Ashruf Omer S
Chondrocytes
Chondrocytes – Drug Effects
Chondrocytes/drug effects
Department of Anatomy & Neurobiology
Fruit
Fruit/chemistry
Gene Expression Regulation/drug effects
Genes – Drug Effects
Haqqi Tariq M
Haseeb Abdul
Humans
I-kappa B Kinase/genetics/*metabolism
IkappaB
IKKbeta
IL-1beta
Interleukin 1 – Metabolism
Interleukin-1beta/metabolism
Interleukin-6/genetics/*metabolism
Interleukins
Interleukins – Metabolism
Khan Nazir M
NEOMED College of Medicine
NF-kappa B
NF-kappa B – Metabolism
NF-kappa B/genetics/*metabolism
NF-kappaB
NIK
Osteoarthritis
Phosphorylation – Drug Effects
Phosphorylation/drug effects
Phytotherapy research : PTR
Plant Extracts
Plant Extracts – Pharmacodynamics
Plant Extracts/chemistry/*pharmacology
Polyphenols – Pharmacodynamics
Polyphenols/pharmacology
Pomegranate
Protein-Serine-Threonine Kinases/genetics/*metabolism
Proteins – Metabolism
Punicaceae/*chemistry
Signal Transduction – Drug Effects
Signal Transduction/drug effects
Transcription Factor RelA/metabolism
Transferases
Transferases – Metabolism