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40
2
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Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1093/chromsci/bmr055" target="_blank" rel="noreferrer noopener">http://doi.org/10.1093/chromsci/bmr055</a>
Pages
271–276
Issue
3
Volume
50
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
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Development and validation of a novel RP-HPLC method for the analysis of reduced glutathione.
Publisher
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Journal of chromatographic science
Date
A point or period of time associated with an event in the lifecycle of the resource
2012
2012-03
Subject
The topic of the resource
Chromatography; Glutathione/*analysis; High Pressure Liquid/*methods; Limit of Detection; Linear Models; Nanoparticles/chemistry; Pharmaceutical Preparations/chemistry; Reproducibility of Results; Reverse-Phase/*methods; Spectrophotometry; Ultraviolet
Creator
An entity primarily responsible for making the resource
Sutariya Vijaykumar; Wehrung Daniel; Geldenhuys Werner J
Description
An account of the resource
The objective of this study was the development, optimization, and validation of a novel reverse-phase high-pressure liquid chromatography (RP-HPLC) method for the quantification of reduced glutathione in pharmaceutical formulations utilizing simple UV detection. The separation utilized a C18 column at room temperature and UV absorption was measured at 215 nm. The mobile phase was an isocratic flow of a 50/50 (v/v) mixture of water (pH 7.0) and acetonitrile flowing at 1.0 mL/min. Validation of the method assessed the methods ability in seven categories: linearity, range, limit of detection, limit of quantification, accuracy, precision, and selectivity. Analysis of the system suitability showed acceptable levels of suitability in all categories. Likewise, the method displayed an acceptable degree of linearity (r(2) = 0.9994) over a concentration range of 2.5-60 microg/mL. The detection limit and quantification limit were 0.6 and 1.8 microg/mL respectively. The percent recovery of the method was 98.80-100.79%. Following validation the method was employed in the determination of glutathione in pharmaceutical formulations in the form of a conjugate and a nanoparticle. The proposed method offers a simple, accurate, and inexpensive way to quantify reduced glutathione.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1093/chromsci/bmr055" target="_blank" rel="noreferrer noopener">10.1093/chromsci/bmr055</a>
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
2012
Chromatography
Geldenhuys Werner J
Glutathione/*analysis
High Pressure Liquid/*methods
Journal of chromatographic science
Limit of Detection
Linear Models
Nanoparticles/chemistry
Pharmaceutical Preparations/chemistry
Reproducibility of Results
Reverse-Phase/*methods
Spectrophotometry
Sutariya Vijaykumar
Ultraviolet
Wehrung Daniel
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1039/c6nr00398b" target="_blank" rel="noreferrer noopener">http://doi.org/10.1039/c6nr00398b</a>
Pages
6542–6554
Issue
12
Volume
8
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Anti-atherogenic effect of trivalent chromium-loaded CPMV nanoparticles in human aortic smooth muscle cells under hyperglycemic conditions in vitro.
Publisher
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Nanoscale
Date
A point or period of time associated with an event in the lifecycle of the resource
2016
2016-03
Subject
The topic of the resource
*Comovirus; Aorta/*metabolism; Atherosclerosis/*drug therapy/therapy; Azo Compounds/chemistry; Cell Proliferation; Cells; Chlorides/*chemistry; Chromium Compounds/*chemistry; Cultured; Cytokines/metabolism; Drug Delivery Systems; Electron; Fluorescence; Glucose/chemistry; Humans; Hyperglycemia/*metabolism; Lipids/chemistry; Microscopy; Myocytes; Nanoparticles/chemistry; NF-kappa B/metabolism; Proliferating Cell Nuclear Antigen/chemistry; Smooth Muscle/*metabolism; Spectrophotometry; Transforming Growth Factor beta/metabolism; Transmission; Ultraviolet
Creator
An entity primarily responsible for making the resource
Ganguly Rituparna; Wen Amy M; Myer Ashley B; Czech Tori; Sahu Soumyadip; Steinmetz Nicole F; Raman Priya
Description
An account of the resource
Atherosclerosis, a major macrovascular complication associated with diabetes, poses a tremendous burden on national health care expenditure. Despite extensive efforts, cost-effective remedies are unknown. Therapies for atherosclerosis are challenged by a lack of targeted drug delivery approaches. Toward this goal, we turn to a biology-derived drug delivery system utilizing nanoparticles formed by the plant virus, Cowpea mosaic virus (CPMV). The aim herein is to investigate the anti-atherogenic potential of the beneficial mineral nutrient, trivalent chromium, loaded CPMV nanoparticles in human aortic smooth muscle cells (HASMC) under hyperglycemic conditions. A non-covalent loading protocol is established yielding CrCl3-loaded CPMV (CPMV-Cr) carrying 2000 drug molecules per particle. Using immunofluorescence microscopy, we show that CPMV-Cr is readily taken up by HASMC in vitro. In glucose (25 mM)-stimulated cells, 100 nM CPMV-Cr inhibits HASMC proliferation concomitant to attenuated proliferating cell nuclear antigen (PCNA, proliferation marker) expression. This is accompanied by attenuation in high glucose-induced phospho-p38 and pAkt expression. Moreover, CPMV-Cr inhibits the expression of pro-inflammatory cytokines, transforming growth factor-beta (TGF-beta) and nuclear factor kappa-light-chain-enhancer of activated B cells (NF-kappaB), in glucose-stimulated HASMCs. Finally glucose-stimulated lipid uptake is remarkably abrogated by CPMV-Cr, revealed by Oil Red O staining. Together, these data provide key cellular evidence for an atheroprotective effect of CPMV-Cr in vascular smooth muscle cells (VSMC) under hyperglycemic conditions that may promote novel therapeutic ventures for diabetic atherosclerosis.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1039/c6nr00398b" target="_blank" rel="noreferrer noopener">10.1039/c6nr00398b</a>
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
*Comovirus
2016
Aorta/*metabolism
Atherosclerosis/*drug therapy/therapy
Azo Compounds/chemistry
Cell Proliferation
Cells
Chlorides/*chemistry
Chromium Compounds/*chemistry
Cultured
Cytokines/metabolism
Czech Tori
Department of Integrative Medical Sciences
Drug Delivery Systems
Electron
Fluorescence
Ganguly Rituparna
Glucose/chemistry
Humans
Hyperglycemia/*metabolism
Lipids/chemistry
Microscopy
Myer Ashley B
Myocytes
Nanoparticles/chemistry
Nanoscale
NEOMED College of Medicine
NF-kappa B/metabolism
Proliferating Cell Nuclear Antigen/chemistry
Raman Priya
Sahu Soumyadip
Smooth Muscle/*metabolism
Spectrophotometry
Steinmetz Nicole F
Transforming Growth Factor beta/metabolism
Transmission
Ultraviolet
Wen Amy M