1
40
2
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1016/s0304-3940(00)01142-3" target="_blank" rel="noreferrer noopener">http://doi.org/10.1016/s0304-3940(00)01142-3</a>
Pages
121–124
Issue
2
Volume
287
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Microglial proliferation in the spinal cord of aged rats with a sciatic nerve injury.
Publisher
An entity responsible for making the resource available
Neuroscience letters
Date
A point or period of time associated with an event in the lifecycle of the resource
2000
2000-06
Subject
The topic of the resource
*Antigens; *Avian Proteins; *Blood Proteins; Aging/*physiology; Animals; Basigin; CD; Cell Division/physiology; Hot Temperature; Hyperalgesia/pathology; Inbred F344; Membrane Glycoproteins/analysis; Microglia/chemistry/*cytology; Neoplasm; Posterior Horn Cells/*cytology; Rats; Sciatic Nerve/*injuries; Sciatica/pathology; Surface
Creator
An entity primarily responsible for making the resource
Stuesse S L; Cruce W L; Lovell J A; McBurney D L; Crisp T
Description
An account of the resource
Nerve injury may lead to chronic neuropathic pain syndromes. We determined whether the extent of central nervous system microglial activation that accompanies nerve injury is age dependent and correlated with behavioral manifestations of pain. We used the Bennett and Xie sciatic nerve chronic constriction injury model (Bennett, G.J., Xie, Y.-K., A peripheral mononeuropathy in rat that produces disorders of pain sensation like those seen in man, Pain, 33 (1998) 87-107) to induce neuropathic pain in three age cohorts of Fischer 344 FBNF1 hybrid rats (4-6, 14-16, and 24-26 months). Rats were assessed for thermal sensitivity (hyperalgesia) of their hind paws pre-injury (day 0) and up to 35 days post injury. On various days post injury, the L4-L5 levels of their spinal cords were reacted for localization of an antibody to OX-42, a marker for microlgia. OX-42 immunoreactivity (ir) was quantified by use of a Bioquant density analysis system. OX-42 ir was heavy in areas of sciatic nerve primary afferent terminations and in the motor columns of its neurons. Aging increases
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1016/s0304-3940(00)01142-3" target="_blank" rel="noreferrer noopener">10.1016/s0304-3940(00)01142-3</a>
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
*Antigens
*Avian Proteins
*Blood Proteins
2000
Aging/*physiology
Animals
Basigin
CD
Cell Division/physiology
Crisp T
Cruce W L
Department of Anatomy & Neurobiology
Hot Temperature
Hyperalgesia/pathology
Inbred F344
Lovell J A
McBurney D L
Membrane Glycoproteins/analysis
Microglia/chemistry/*cytology
NEOMED College of Medicine
Neoplasm
Neuroscience letters
Posterior Horn Cells/*cytology
Rats
Sciatic Nerve/*injuries
Sciatica/pathology
Stuesse S L
Surface
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1016/j.canlet.2016.05.014" target="_blank" rel="noreferrer noopener">http://doi.org/10.1016/j.canlet.2016.05.014</a>
Pages
131–141
Issue
2
Volume
378
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Lipopolysaccharide supports maintaining the stemness of CD133(+) hepatoma cells through activation of the NF-kappaB/HIF-1alpha pathway.
Publisher
An entity responsible for making the resource available
Cancer letters
Date
A point or period of time associated with an event in the lifecycle of the resource
2016
2016-08
Subject
The topic of the resource
*Cancer stem cells; *Lipopolysaccharide; *Plasticity; *Stemness maintenance; *Tumor microenvironment; AC133 Antigen/genetics/*metabolism; alpha Subunit/genetics/*metabolism; Animals; Antineoplastic Agents – Pharmacodynamics; Antineoplastic Agents/pharmacology; Body Weights and Measures; Carcinoma; Cell Line; Cell Movement – Drug Effects; Cell Movement/drug effects; Cell Physiology; Cell Physiology – Drug Effects; Cell Proliferation/drug effects; Drug Resistance; Gene Expression Regulation; Genes; Genetic Techniques; Hepatocellular; Hepatocellular – Drug Therapy; Hepatocellular – Metabolism; Hepatocellular – Pathology; Hepatocellular/drug therapy/genetics/*metabolism/pathology; Humans; Hypoxia-Inducible Factor 1; Inbred BALB C; Lipopolysaccharides – Pharmacodynamics; Lipopolysaccharides/*pharmacology; Liver Neoplasms; Liver Neoplasms – Drug Therapy; Liver Neoplasms – Metabolism; Liver Neoplasms – Pathology; Liver Neoplasms/drug therapy/genetics/*metabolism/pathology; Male; Mice; Neoplasm; Neoplasm Invasiveness; Neoplastic; Neoplastic Stem Cells/*drug effects/metabolism/pathology; NF-kappa B – Metabolism; NF-kappa B/*metabolism; Nude; Phenotype; Proteins; Proteins – Metabolism; RNA Interference; Signal Transduction – Drug Effects; Signal Transduction/drug effects; Stem Cells – Drug Effects; Stem Cells – Metabolism; Stem Cells – Pathology; Time Factors; Transfection; Tumor Burden; Tumor Microenvironment
Creator
An entity primarily responsible for making the resource
Lai Fo-Bao; Liu Wen-Ting; Jing Ying-Ying; Yu Guo-Feng; Han Zhi-Peng; Yang Xue; Zeng Jian-Xing; Zhang Hang-Jie; Shi Rong-Yu; Li Xiao-Yong; Pan Xiao-Rong; Li Rong; Zhao Qiu-Dong; Wu Meng-Chao; Zhang Ping; Liu Jing-Feng; Wei Li-Xin
Description
An account of the resource
Due to the existence of cancer stem cells (CSCs), persistence and relapse of human hepatocellular carcinoma (HCC) are common after treatment with existing anti-cancer therapies. Emerging evidence indicates that lipopolysaccharide (LPS) plays a crucial role in aggravating HCC, but information about the effect of LPS on CSCs of HCC remains scant. Here, we report that the stemness of CD133(+) CSCs sorted from the human HCC cell line Huh7 was maintained well when cells were cultured with LPS. The reduction of CD133 expression was much lesser in cultured CSCs in the presence of LPS. In response to LPS stimulation, CSCs showed an increase in their activity of clonogenesis and tumorigenesis. LPS also supported maintaining CSC abilities of migration, invasion, and chemo-resistance. Treatment with HIF-1alpha-specific siRNA significantly reduced CD133 expression by CSCs at both mRNA and protein levels. Further, the expression of HIF-1alpha and CD133 was reduced in LPS-stimulated CSCs when the NF-kappaB inhibitor was added to the cell culture. HIF-1alpha-specific siRNA also effectively counteracted the effect of LPS on maintaining CSC abilities of migration and invasion. These data indicate that LPS, an important mediator in the liver tumor microenvironment, supports the maintenance of CSC stemness through signaling of the NF-kappaB/HIF-1alpha pathway. Our current study highlights LPS as a potential target for developing new therapeutic approaches to eliminate CSCs during the treatment of HCC.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1016/j.canlet.2016.05.014" target="_blank" rel="noreferrer noopener">10.1016/j.canlet.2016.05.014</a>
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
*Cancer stem cells
*Lipopolysaccharide
*Plasticity
*Stemness maintenance
*Tumor microenvironment
2016
AC133 Antigen/genetics/*metabolism
alpha Subunit/genetics/*metabolism
Animals
Antineoplastic Agents – Pharmacodynamics
Antineoplastic Agents/pharmacology
Body Weights and Measures
Cancer letters
Carcinoma
Cell Line
Cell Movement – Drug Effects
Cell Movement/drug effects
Cell Physiology
Cell Physiology – Drug Effects
Cell Proliferation/drug effects
Department of Integrative Medical Sciences
Drug Resistance
Gene Expression Regulation
Genes
Genetic Techniques
Han Zhi-Peng
Hepatocellular
Hepatocellular – Drug Therapy
Hepatocellular – Metabolism
Hepatocellular – Pathology
Hepatocellular/drug therapy/genetics/*metabolism/pathology
Humans
Hypoxia-Inducible Factor 1
Inbred BALB C
Jing Ying-Ying
Lai Fo-Bao
Li Rong
Li Xiao-Yong
Lipopolysaccharides – Pharmacodynamics
Lipopolysaccharides/*pharmacology
Liu Jing-Feng
Liu Wen-Ting
Liver Neoplasms
Liver Neoplasms – Drug Therapy
Liver Neoplasms – Metabolism
Liver Neoplasms – Pathology
Liver Neoplasms/drug therapy/genetics/*metabolism/pathology
Male
Mice
NEOMED College of Medicine
Neoplasm
Neoplasm Invasiveness
Neoplastic
Neoplastic Stem Cells/*drug effects/metabolism/pathology
NF-kappa B – Metabolism
NF-kappa B/*metabolism
Nude
Pan Xiao-Rong
Phenotype
Proteins
Proteins – Metabolism
RNA Interference
Shi Rong-Yu
Signal Transduction – Drug Effects
Signal Transduction/drug effects
Stem Cells – Drug Effects
Stem Cells – Metabolism
Stem Cells – Pathology
Time Factors
Transfection
Tumor Burden
Tumor Microenvironment
Wei Li-Xin
Wu Meng-Chao
Yang Xue
Yu Guo-Feng
Zeng Jian-Xing
Zhang Hang-Jie
Zhang Ping
Zhao Qiu-Dong