Anti-Inflammatory Mechanism Involved in Pomegranate-Mediated Prevention of Breast Cancer: the Role of NF-kappaB and Nrf2 Signaling Pathways.
Female; Animals; Anti-Inflammatory Agents/*pharmacology; Rats; Gene Expression Regulation; Signal Transduction; Apoptosis/drug effects; anti-inflammatory effects; Anticarcinogenic Agents/pharmacology; breast tumor; COX-2; Cyclooxygenase 2/genetics/metabolism; DMBA; HSP90; HSP90 Heat-Shock Proteins/genetics/metabolism; I-kappa B Kinase/genetics/metabolism; NF-E2-Related Factor 2/genetics/*metabolism; NF-kappa B/genetics/*metabolism; NF-kappaB; Nrf2; Plant Preparations/*pharmacology; Punica granatum; Punicaceae/*chemistry; Pomegranate; Chemoprevention; Dose-Response Relationship; Drug; Neoplastic; Mammary Neoplasms; 10-Dimethyl-1; 9; Neoplastic/drug effects; Cell Transformation; 2-benzanthracene/toxicity; Experimental/*prevention & control; Animal Studies; Breast Neoplasms; Inflammation Mediators; Physical Education and Training; Neoplasms – Prevention and Control
Pomegranate (Punica granatum L.), a nutrient-rich unique fruit, has been used for centuries for the prevention and treatment of various inflammation-driven diseases. Based on our previous study, a characterized pomegranate emulsion (PE) exhibited a striking inhibition of dimethylbenz(a)anthracene (DMBA)-initiated rat mammary tumorigenesis via antiproliferative and apoptosis-inducing mechanisms. The objective of the present work is to investigate the anti-inflammatory mechanism of action of PE during DMBA rat mammary carcinogenesis by evaluating the expression of cyclooxygenase-2 (COX-2), heat shock protein 90 (HSP90), nuclear factor-kappaB (NF-kappaB) and nuclear factor erythroid 2p45 (NF-E2)-related factor 2 (Nrf2). Mammary tumor samples were harvested from our previous chemopreventive study in which PE (0.2-5.0 g/kg) was found to reduce mammary tumorigenesis in a dose-dependent manner. The expressions of COX-2, HSP90, NF-kappaB, inhibitory kappaBalpha (IkappaBalpha) and Nrf2 were detected by immunohistochemical techniques. PE decreased the expression of COX-2 and HSP90, prevented the degradation of IkappaBalpha, hindered the translocation of
Mandal Animesh; Bhatia Deepak; Bishayee Anupam
Nutrients
2017
2017-04
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
<a href="http://doi.org/10.3390/nu9050436" target="_blank" rel="noreferrer noopener">10.3390/nu9050436</a>
Chemopreventive and therapeutic potential of tea polyphenols in hepatocellular cancer.
*Anticarcinogenic Agents; Animal; Animals; Anti-Inflammatory Agents; Antioxidants; Biological Availability; Biological Markers; Carcinoma; Catechin/administration & dosage/analogs & derivatives; Chemoprevention; Disease Models; Hepatocellular – Physiopathology; Hepatocellular – Prevention and Control; Hepatocellular – Therapy; Human; Humans; In Vitro Studies; In Vivo Studies; Liver Neoplasms/*drug therapy/*prevention & control; Mice; Neoplasms – Prevention and Control; Nutrition; Outcomes (Health Care); Phenols – Therapeutic Use; Polyphenols/*administration & dosage/pharmacology; Tea – Therapeutic Use; Tea/*chemistry; Xenograft Model Antitumor Assays
The prophylactic and therapeutic properties of tea have been attributed to green tea catechins and black tea theaflavins besides several other polyphenolic compounds. Tea polyphenols possess potent antioxidant and antiinflammatory properties and modulate several signaling pathways. These biochemical facets of tea polyphenols are responsible for its anticancer properties. Several lethal cancers, such as liver cancer, develop within a background of oxidative stress and inflammation. Liver cancer, also known as hepatocellular carcinoma (HCC), has been shown to occur throughout the world including Asia, Africa, Western Europe, and the United States. Phytochemicals, such as tea polyphenols, provide an effective and promising alternative for the chemoprevention and treatment of HCC. In this article, we systematically review, for the first time, the effects of tea polyphenols in the preclinical in vitro and in vivo HCC models. The review also examines, in critical detail, the biochemical mechanisms involved in the chemopreventive and antineoplastic effects of tea polyphenols in hepatic cancer. Finally, we highlight the role of synergy, bioavailability and pharmacokinetics of tea polyphenols, current status of clinical trials, discuss future directions, and comment on the future challenges involved in the effective use of tea polyphenols for the prevention and management of liver cancer.
Darvesh Altaf S; Bishayee Anupam
Nutrition and cancer
2013
1905-07
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
<a href="http://doi.org/10.1080/01635581.2013.767367" target="_blank" rel="noreferrer noopener">10.1080/01635581.2013.767367</a>
Pomegranate exerts chemoprevention of experimentally induced mammary tumorigenesis by suppression of cell proliferation and induction of apoptosis.
*Punicaceae/chemistry; 10-Dimethyl-1; 2-benzanthracene; 9; Animal Studies; Animals; Anticarcinogenic Agents/*therapeutic use; Apoptosis; Apoptosis/*drug effects; Breast Neoplasms – Mortality; Breast Neoplasms – Prevention and Control; Breast Neoplasms – Risk Factors; Cell Physiology; Cell Proliferation/*drug effects; Diet – Evaluation; Experimental/pathology/*prevention & control; Female; Funding Source; Gene Expression Regulation; Genes; Human; Immunohistochemistry; Mammary Neoplasms; Mutation; National Institutes of Health (U.S.); Neoplasms – Prevention and Control; Phytotherapy; Pomegranate; Proto-Oncogene Proteins c-bcl-2/analysis; Rats; Sprague-Dawley; Tamoxifen; United States
Breast cancer is the second leading cause of cancer-related death in women in the United States and discovery and development of safe chemopreventive drugs is urgently needed. The fruit pomegranate (Punica granatum) is gaining importance because of its various health benefits. This study was initiated to investigate chemopreventive potential of a pomegranate emulsion (PE) against 7,12-dimethylbenz(a)anthracene (DMBA) rat mammary carcinogenesis. The animals were orally administered with PE (0.2-5.0 g/kg), starting 2 wk before and 16 wk following DMBA treatment. PE exhibited a striking reduction of DMBA-induced mammary tumor incidence, total tumor burden, and reversed histopathological changes. PE dose-dependently suppressed cell proliferation and induced apoptosis in mammary tumors. Immunohistochemical studies showed that PE increased intratumor Bax, decreased Bcl2 and manifested a proapoptotic shift in Bax/Bcl2 ratio. In addition, our gene expression study showed PE-mediated upregulation of Bad, caspase-3, caspase-7, caspase-9, poly (ADP ribose) polymerase and cytochrome c in mammary tumors. Thus, PE exerts chemoprevention of mammary carcinogenesis by suppressing cell proliferation and inducing apoptosis mediated through upregulation of Bax and downregulation of Bcl2 in concert with caspase cascades. Pomegranate bioactive phytoconstituents could be developed as a chemopreventive drug to reduce the risk of breast cancer.
Bishayee Anupam; Mandal Animesh; Bhattacharyya Piyali; Bhatia Deepak
Nutrition and cancer
2016
1905-07
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
<a href="http://doi.org/10.1080/01635581.2016.1115094" target="_blank" rel="noreferrer noopener">10.1080/01635581.2016.1115094</a>
Vanadium in the detection, prevention and treatment of cancer: the in vivo evidence.
10-Dimethyl-1; 2-benzanthracene; 9; Animal; Animals; Biological; Breast Neoplasms – Chemically Induced; Breast Neoplasms – Diagnosis; Breast Neoplasms – Drug Therapy; Cell Division – Drug Effects; Cell Division/drug effects; Disease Models; Experimental/chemically induced/diagnosis/drug therapy; Experimental/drug therapy/pathology; Female; Glioblastoma/drug therapy; Glioma – Drug Therapy; Heterologous; Humans; Hydrocarbons; Male; Mammary Neoplasms; Metals; Metals – Diagnostic Use; Metals – Therapeutic Use; Models; Neoplasm Staging; Neoplasm Transplantation; Neoplasms; Neoplasms – Diagnosis; Neoplasms – Drug Therapy; Neoplasms – Pathology; Neoplasms – Prevention and Control; Neoplasms/*diagnosis/drug therapy/prevention & control; Organometallic Compounds – Therapeutic Use; Organometallic Compounds/therapeutic use; Rats; Trace Elements – Diagnostic Use; Trace Elements – Therapeutic Use; Trace Elements/therapeutic use; Transplantation; Vanadium Compounds; Vanadium Compounds – Therapeutic Use; Vanadium Compounds/therapeutic use/toxicity; Vanadium/*therapeutic use/toxicity; Xenografts
Vanadium, a dietary micronutrient, is yet to be established as an essential part of the human diet. Over the past century, several biological effects of vanadium, such as insulin-mimetic action as well as amelioration of hyperlipidemia and hypertension, have been discovered. This transition element is known to influence a battery of enzymatic systems, namely phosphatases, ATPases, peroxidases, ribonucleases, protein kinases and oxidoreductases. Multiple biochemical and molecular actions of vanadium have been implicated in its inhibitory effects on various tumor cells of human origin. Successful in vitro studies over the past few decades have advanced the anticancer research on vanadium into the preclinical stage. Vanadium in several animal cancer models provides protection against all stages of carcinogenesis–initiation, promotion, and progression. This review focuses on the current advances in cancer prevention and treatment as well as early detection by vanadium compounds in preclinical animal models while pointing to possible mechanisms of such diverse beneficial effects. Clinical pharmacokinetic and potential toxicity studies on vanadium are also highlighted in this review. Supporting and challenging evidence as well as future directions of vanadium research exploring the possibility of using this dietary agent for detection, prevention and treatment of human cancers are critically discussed.
Bishayee Anupam; Waghray Abhijeet; Patel Mehool A; Chatterjee Malay
Cancer letters
2010
2010-08
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
<a href="http://doi.org/10.1016/j.canlet.2010.01.030" target="_blank" rel="noreferrer noopener">10.1016/j.canlet.2010.01.030</a>