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40
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Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1128/JVI.02210-10" target="_blank" rel="noreferrer noopener">http://doi.org/10.1128/JVI.02210-10</a>
Pages
2060–2078
Issue
5
Volume
85
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Retrovirus-induced spongiform neurodegeneration is mediated by unique central nervous system viral targeting and expression of env alone.
Publisher
An entity responsible for making the resource available
Journal of virology
Date
A point or period of time associated with an event in the lifecycle of the resource
2011
2011-03
Subject
The topic of the resource
Animals; Cell Line; Friend murine leukemia virus/genetics/metabolism; Humans; Leukemia Virus; Mice; Murine/genetics/*metabolism/pathogenicity; Nerve Degeneration; Neural Stem Cells/pathology/virology; Neurodegenerative Diseases/pathology/*virology; Retroviridae Infections/pathology/*virology; Viral Envelope Proteins/genetics/*metabolism; Virulence
Creator
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Li Ying; Cardona Sandra M; Traister Russell S; Lynch William P
Description
An account of the resource
Certain murine leukemia viruses (MLVs) can induce progressive noninflammatory spongiform neurodegeneration similar to that caused by prions. The primary MLV determinants responsible have been mapped to within the env gene; however, it has remained unclear how env mediates disease, whether non-Env viral components are required, and what central nervous system (CNS) cells constitute the critical CNS targets. To address these questions, we examined the effect of transplanting engraftable C17.2 neural stem cells engineered to pseudotype, disseminate, and trans-complement neurovirulent (CasBrE, CasE, and CasES) or non-neurovirulent (Friend and SFF-FE) env sequences (SU or SU/TM) within the CNS using either the "non-neurovirulent" amphotropic helper virus, 4070A, or pgag-polgpt (a nonpackaged vector encoding Gag-Pol). These studies revealed that acute
Identifier
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<a href="http://doi.org/10.1128/JVI.02210-10" target="_blank" rel="noreferrer noopener">10.1128/JVI.02210-10</a>
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
2011
Animals
Cardona Sandra M
Cell Line
Department of Integrative Medical Sciences
Friend murine leukemia virus/genetics/metabolism
Humans
Journal of virology
Leukemia Virus
Li Ying
Lynch William P
Mice
Murine/genetics/*metabolism/pathogenicity
NEOMED College of Medicine
Nerve Degeneration
Neural Stem Cells/pathology/virology
Neurodegenerative Diseases/pathology/*virology
Retroviridae Infections/pathology/*virology
Traister Russell S
Viral Envelope Proteins/genetics/*metabolism
Virulence
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
Pages
6841–6851
Issue
8
Volume
73
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Neural stem cells as engraftable packaging lines can mediate gene delivery to microglia: evidence from studying retroviral env-related neurodegeneration.
Publisher
An entity responsible for making the resource available
Journal of virology
Date
A point or period of time associated with an event in the lifecycle of the resource
1999
1999-08
Subject
The topic of the resource
Animals; Mice; Cell Line; *Gene Transfer Techniques; Retroviridae Infections/pathology/*virology; *Microglia/cytology; *Stem Cells/cytology; Hematopoietic Stem Cell Transplantation; Nerve Degeneration; Retroviridae/genetics/*physiology; *Genes; env
Creator
An entity primarily responsible for making the resource
Lynch W P; Sharpe A H; Snyder E Y
Description
An account of the resource
The induction of spongiform myeloencephalopathy by murine leukemia viruses is mediated primarily by infection of central nervous system (CNS) microglia. In this regard, we have previously shown that CasBrE-induced disease requires late, rather than early, virus replication events in microglial cells (W. P. Lynch et al., J. Virol. 70:8896-8907, 1996). Furthermore, neurodegeneration requires the presence of unique sequences within the viral env gene. Thus, the neurodegeneration-inducing events could result from microglial expression of retroviral envelope protein alone or from the interaction of envelope protein with other viral structural proteins in the virus assembly and maturation process. To distinguish between these possible mechanisms of disease induction, we engineered the engraftable neural stem cell line C17-2 into packaging/producer cells in order to deliver the neurovirulent CasBrE env gene to endogenous CNS cells. This strategy resulted in significant CasBrE env expression within CNS microglia without the appearance of replication competent virus. CasBrE envelope expression within microglia was accompanied by increased expression of activation markers F4/80 and Mac-1 (CD11b) but failed to induce spongiform neurodegenerative changes. These results suggest that envelope expression alone within microglia is not sufficient to induce neurodegeneration. Rather, microglia-mediated disease appears to require neurovirulent Env protein interaction with other viral proteins during assembly or maturation. More broadly, the results presented here prove the efficacy of a novel method by which neural stem cell biology may be harnessed for genetically manipulating the CNS, not only for studying neurodegeneration but also as a paradigm for the disseminated distribution of retroviral vector-transduced genes.
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
*Gene Transfer Techniques
*Genes
*Microglia/cytology
*Stem Cells/cytology
1999
Animals
Cell Line
env
Hematopoietic Stem Cell Transplantation
Journal of virology
Lynch W P
Mice
Nerve Degeneration
Retroviridae Infections/pathology/*virology
Retroviridae/genetics/*physiology
Sharpe A H
Snyder E Y