1
40
2
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1016/j.msec.2014.12.062" target="_blank" rel="noreferrer noopener">http://doi.org/10.1016/j.msec.2014.12.062</a>
Pages
663–672
Volume
48
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Dewetting based fabrication of fibrous micro-scaffolds as potential injectable cell carriers.
Publisher
An entity responsible for making the resource available
Materials science & engineering. C, Materials for biological applications
Date
A point or period of time associated with an event in the lifecycle of the resource
2015
2015-03
Subject
The topic of the resource
Animals; Cell retention; Cells; Dewetting; Fibrous micro-constructs; Immobilized/metabolism/transplantation; Injectable constructs; Myocardial Infarction/metabolism/pathology/*therapy; Rats; Sprague-Dawley; Stem Cell Transplantation/*methods; Stem Cells/*metabolism; Tissue engineering and regeneration; Tissue Scaffolds/*chemistry
Creator
An entity primarily responsible for making the resource
Song Hokyung; Yin Liya; Chilian William M; Newby Bi-Min Zhang
Description
An account of the resource
Although regenerative medicine utilizing tissue scaffolds has made enormous strides in recent years, many constraints still hamper their effectiveness. A limitation of many scaffolds is that they form surface patches, which are not particularly effective for some types of "wounds" that are deep within tissues, e.g., stroke and myocardial infarction. In this study, we reported the generation of fibrous micro-scaffolds feasible for delivering cells by injection into the tissue parenchyma. The micro-scaffolds (widths\textless100mum) were made by dewetting of poly(lactic-co-glycolic acid) thin films containing parallel strips, and cells were seeded to form cell/polymer micro-constructs during or post the micro-scaffold fabrication process. Five types of cells including rat induced vascular progenitor cells were assessed for the formation of the micro-constructs. Critical factors in forming fibrous micro-scaffolds via dewetting of polymer thin films were found to be properties of polymers and supporting substrates, temperature, and proteins in the culture medium. Also, the ability of cells to attach to the micro-scaffolds was essential in forming cell/polymer micro-constructs. Both in vitro and in vivo assessments of injecting these micro-scaffolding constructs showed, as compared to free cells, enhanced cell retention at the injected site, which could lead to improved tissue engineering and regeneration.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1016/j.msec.2014.12.062" target="_blank" rel="noreferrer noopener">10.1016/j.msec.2014.12.062</a>
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
2015
Animals
Cell retention
Cells
Chilian William M
Department of Integrative Medical Sciences
Dewetting
Fibrous micro-constructs
Immobilized/metabolism/transplantation
Injectable constructs
Materials science & engineering. C, Materials for biological applications
Myocardial Infarction/metabolism/pathology/*therapy
NEOMED College of Medicine
Newby Bi-Min Zhang
Rats
Song Hokyung
Sprague-Dawley
Stem Cell Transplantation/*methods
Stem Cells/*metabolism
Tissue engineering and regeneration
Tissue Scaffolds/*chemistry
Yin Liya
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1007/s00395-017-0631-4" target="_blank" rel="noreferrer noopener">http://doi.org/10.1007/s00395-017-0631-4</a>
Pages
41–41
Issue
4
Volume
112
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
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Alignment of inducible vascular progenitor cells on a micro-bundle scaffold improves cardiac repair following myocardial infarction.
Publisher
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Basic research in cardiology
Date
A point or period of time associated with an event in the lifecycle of the resource
2017
2017-07
Subject
The topic of the resource
*Cardiovascular regeneration; *Ischemic heart diseases; *Micro-bundle scaffold; *Myocardial infarction; *Neovascularization; *Stem cells; *Tissue Scaffolds; *Vascular progenitor cells; Animal; Animals; Cell Differentiation; Cell Proliferation; Cell Survival; Cells; Coculture Techniques; Cultured; Disease Models; Endothelial Progenitor Cells/metabolism/*transplantation; Fibroblast Growth Factor 2/metabolism; Lactic Acid/*chemistry; Muscle; Myocardial Infarction/metabolism/pathology/physiopathology/*surgery; Myocardium/metabolism/*pathology; Myocytes; Paracrine Communication; Phenotype; Physiologic; Polyglycolic Acid/*chemistry; Polylactic Acid-Polyglycolic Acid Copolymer; Rats; Signal Transduction; Smooth; Smooth Muscle/metabolism/*transplantation; Sprague-Dawley; Time Factors; Tissue Engineering/*methods; Vascular Endothelial Growth Factor A/metabolism; Vascular/metabolism/*transplantation; Ventricular Remodeling
Creator
An entity primarily responsible for making the resource
Jamaiyar Anurag; Wan Weiguo; Ohanyan Vahagn; Enrick Molly; Janota Danielle; Cumpston Devan; Song Hokyung; Stevanov Kelly; Kolz Christopher L; Hakobyan Tatev; Dong Feng; Newby Bi-Min Zhang; Chilian William M; Yin Liya
Description
An account of the resource
Ischemic heart disease is still the leading cause of death even with the advancement of pharmaceutical therapies and surgical procedures. Early vascularization in the ischemic heart is critical for a better outcome. Although stem cell therapy has great potential for cardiovascular regeneration, the ideal cell type and delivery method of cells have not been resolved. We tested a new approach of stem cell therapy by delivery of induced vascular progenitor cells (iVPCs) grown on polymer micro-bundle scaffolds in a rat model of myocardial infarction. iVPCs partially reprogrammed from vascular endothelial cells (ECs) had potent angiogenic potential and were able to simultaneously differentiate into vascular smooth muscle cells (SMCs) and ECs in 2D culture. Under hypoxic conditions, iVPCs also secreted angiogenic cytokines such as vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF) as measured by enzyme-linked immunosorbent assay (ELISA). A longitudinal micro-scaffold made from poly(lactic-co-glycolic acid) was sufficient for the growth and delivery of iVPCs. Co-cultured ECs and SMCs aligned well on the micro-bundle scaffold similarly as in the vessels. 3D cell/polymer micro-bundles formed by iVPCs and micro-scaffolds were transplanted into the ischemic myocardium in a rat model of myocardial infarction (MI) with ligation of the left anterior descending artery. Our in vivo data showed that iVPCs on the micro-bundle scaffold had higher survival, and better retention and engraftment in the myocardium than free iVPCs. iVPCs on the micro-bundles promoted better cardiomyocyte survival than free iVPCs. Moreover, iVPCs and iVPC/polymer micro-bundles treatment improved cardiac function (ejection fraction and fractional shortening, endocardial systolic volume) measured by echocardiography, increased vessel density, and decreased infarction size [endocardial and epicardial infarct (scar) length] better than untreated controls at 8 weeks after MI. We conclude that iVPCs grown on a polymer micro-bundle scaffold are new promising approach for cell-based therapy designed for cardiovascular regeneration in ischemic heart disease.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1007/s00395-017-0631-4" target="_blank" rel="noreferrer noopener">10.1007/s00395-017-0631-4</a>
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
*Cardiovascular regeneration
*Ischemic heart diseases
*Micro-bundle scaffold
*Myocardial infarction
*Neovascularization
*Stem cells
*Tissue Scaffolds
*Vascular progenitor cells
2017
Animal
Animals
Basic research in cardiology
Cell Differentiation
Cell Proliferation
Cell Survival
Cells
Chilian William M
Coculture Techniques
Cultured
Cumpston Devan
Department of Integrative Medical Sciences
Disease Models
Dong Feng
Endothelial Progenitor Cells/metabolism/*transplantation
Enrick Molly
Fibroblast Growth Factor 2/metabolism
Hakobyan Tatev
Jamaiyar Anurag
Janota Danielle
Kolz Christopher L
Lactic Acid/*chemistry
Muscle
Myocardial Infarction/metabolism/pathology/physiopathology/*surgery
Myocardium/metabolism/*pathology
Myocytes
NEOMED College of Medicine
Newby Bi-Min Zhang
Ohanyan Vahagn
Paracrine Communication
Phenotype
Physiologic
Polyglycolic Acid/*chemistry
Polylactic Acid-Polyglycolic Acid Copolymer
Rats
Signal Transduction
Smooth
Smooth Muscle/metabolism/*transplantation
Song Hokyung
Sprague-Dawley
Stevanov Kelly
Time Factors
Tissue Engineering/*methods
Vascular Endothelial Growth Factor A/metabolism
Vascular/metabolism/*transplantation
Ventricular Remodeling
Wan Weiguo
Yin Liya