1
40
1
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1016/j.bbrc.2004.02.029" target="_blank" rel="noreferrer noopener">http://doi.org/10.1016/j.bbrc.2004.02.029</a>
Pages
158–164
Issue
1
Volume
316
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Transcriptional regulation of human oxysterol 7alpha-hydroxylase by sterol response element binding protein.
Publisher
An entity responsible for making the resource available
Biochemical and biophysical research communications
Date
A point or period of time associated with an event in the lifecycle of the resource
2004
2004-03
Subject
The topic of the resource
*Transcription Factors; Base Sequence; CCAAT-Enhancer-Binding Proteins/*metabolism; Cytochrome P-450 Enzyme System/*genetics/physiology; Cytochrome P450 Family 7; DNA-Binding Proteins/*metabolism; Enzyme Repression; Genetic; Humans; Molecular Sequence Data; Mutagenesis; Promoter Regions; Response Elements; Sp1 Transcription Factor/antagonists & inhibitors; Steroid Hydroxylases/*genetics/physiology; Sterol Regulatory Element Binding Protein 1; Sterols/metabolism; Transcription
Creator
An entity primarily responsible for making the resource
Norlin Maria; Chiang John Y L
Description
An account of the resource
Oxysterol 7alpha-hydroxylase (CYP7B1) metabolizes oxysterols, potent regulators of lipid homeostasis. Very little is known about transcriptional regulation of human CYP7B1. The present results indicate that sterol response element binding protein (SREBP), a family of oxysterol-responsive transcription factors that stimulates cholesterol synthesis, may be an important regulator of CYP7B1. SREBP suppressed a human CYP7B1 luciferase reporter gene in several cell lines, most markedly in rat hepatoma McA-RH7777 cells. An SREBP-1-responsive region was mapped to a GC-rich sequence in the proximal CYP7B1 promoter, containing binding sites for the basal transcriptional activator Sp1. Mutagenesis of this sequence abolished SREBP-1-mediated suppression. Data indicated that SREBP does not bind this sequence but affects the gene indirectly, probably via interaction with Sp1. Our findings indicate that CYP7B1 transcription is controlled by SREBP and reveal a link between oxysterol-sensitive regulators and oxysterol metabolism. We propose that CYP7B1 is important for regulating cellular sterol content and protects against oxysterol-mediated toxicity.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1016/j.bbrc.2004.02.029" target="_blank" rel="noreferrer noopener">10.1016/j.bbrc.2004.02.029</a>
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
*Transcription Factors
2004
Base Sequence
Biochemical and biophysical research communications
CCAAT-Enhancer-Binding Proteins/*metabolism
Chiang John Y L
Cytochrome P-450 Enzyme System/*genetics/physiology
Cytochrome P450 Family 7
Department of Integrative Medical Sciences
DNA-Binding Proteins/*metabolism
Enzyme Repression
Genetic
Humans
Molecular Sequence Data
Mutagenesis
NEOMED College of Medicine
Norlin Maria
Promoter Regions
Response Elements
Sp1 Transcription Factor/antagonists & inhibitors
Steroid Hydroxylases/*genetics/physiology
Sterol Regulatory Element Binding Protein 1
Sterols/metabolism
Transcription