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Text
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URL Address
<a href="http://doi.org/10.1016/0091-3057(88)90102-5" target="_blank" rel="noreferrer noopener">http://doi.org/10.1016/0091-3057(88)90102-5</a>
Pages
795–799
Issue
3
Volume
30
Dublin Core
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Title
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A rapid and simple HPLC microassay for biogenic amines in discrete brain regions.
Publisher
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Pharmacology, biochemistry, and behavior
Date
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1988
1988-07
Subject
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Male; Animals; Rats; Reference Values; Organ Specificity; *Brain Chemistry; Chromatography; Biogenic Amines/*analysis; Corpus Striatum/analysis; Indicators and Reagents; Nucleus Accumbens/analysis; Inbred Strains; High Pressure Liquid/methods
Creator
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Donzanti B A; Yamamoto B K
Description
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A rapid microassay is described for the measurement of biogenic amines using an isocratic HPLC system with electrochemical detection. Catecholamines, indoleamines and their major metabolites were extracted with 150 microliters of perchloric acid from brain tissue punches (less than 250 micrograms) using a simple one-step sample preparation method. These compounds were separated on a short (80 mm) column with 3 microns particle size packing, and electrochemically detected within a total run time of less than 6 minutes. Detection limit sensitivity was approximately 2-5 pg. This method, detailed in an easy-to-follow description, reduces assay time, minimizes the possibility for errors, maximizes efficiency, and requires only standard HPLC equipment and supplies.
Identifier
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<a href="http://doi.org/10.1016/0091-3057(88)90102-5" target="_blank" rel="noreferrer noopener">10.1016/0091-3057(88)90102-5</a>
Rights
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Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
*Brain Chemistry
1988
Animals
Biogenic Amines/*analysis
Chromatography
Corpus Striatum/analysis
Donzanti B A
High Pressure Liquid/methods
Inbred Strains
Indicators and Reagents
Male
Nucleus Accumbens/analysis
Organ Specificity
Pharmacology, biochemistry, and behavior
Rats
Reference Values
Yamamoto B K