1 40 2 Text A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text. URL Address <a href="http://doi.org/10.1007/978-1-0716-1119-7_15" target="_blank" rel="noreferrer noopener">http://doi.org/10.1007/978-1-0716-1119-7_15</a> Pages 215-224 Volume 2245 ISSN 1940-6029 1064-3745 Search for Full-text Locate full-text within NEOMED Library's e-journal collections <a href="http://neomed.idm.oclc.org/login?url=http://doi.org/10.1007/978-1-0716-1119-7_15" target="_blank" rel="noreferrer noopener">NEOMED Full-text Holding (if available) - Proxy DOI: 10.1007/978-1-0716-1119-7_15</a> Users with a NEOMED Library login can search for full-text journal articles at the following url: <a href="https://libraryguides.neomed.edu/home">https://libraryguides.neomed.edu/home</a> Update Year & Number January 2021 List NEOMED College NEOMED College of Medicine NEOMED Department Department of Anatomy & Neurobiology Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Assessing chondrocyte status by immunofluorescence-mediated localization of parkin relative to mitochondria. Publisher An entity responsible for making the resource available Methods in Molecular Biology Date A point or period of time associated with an event in the lifecycle of the resource 2021 1905-07 Subject The topic of the resource Inflammation; Mitochondria; Oxidative stress; Osteoarthritis; Chondrocytes; Inflammation; Osteoarthritis; Autophagy; CCCP; Confocal microscopy; IL-1β; Immunofluorescence; Mitochondrial dysfunction; Mitophagy; MitoTracker Deep Red; Parkin; PINK1; Autophagy; CCCP; Chondrocytes; Confocal microscopy; IL-1β; Immunofluorescence; Mitochondria; Mitochondrial dysfunction; Mitophagy; MitoTracker Deep Red; Oxidative stress; Parkin; PINK1 Creator An entity primarily responsible for making the resource Ansari MY; Haqqi TM Description An account of the resource Immunofluorescence staining is a widely used and powerful tool for the visualization and colocalization of two or more proteins and/or cellular organelles. For colocalization studies in fixed cells, one target protein/organelle is immunostained and visualized by one fluorophore and the other target protein/organelle is immunostained and visualized by a different fluorophore whose excitation emission spectra does not overlap with the first fluorophore. Parkin (PARK2) is an E3 ubiquitin ligase which performs ubiquitination of surface proteins of dysfunctional mitochondria to mark them for autolysosomal degradation. Here we describe the immunofluorescence staining of parkin protein and immunofluorescence or dye-based methods to visualize mitochondria and study the colocalization of parkin and mitochondria in primary human or mouse chondrocytes or cell lines. Identifier An unambiguous reference to the resource within a given context <a href="http://doi.org/10.1007/978-1-0716-1119-7_15" target="_blank" rel="noreferrer noopener">10.1007/978-1-0716-1119-7_15</a> Rights Information about rights held in and over the resource Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s). Format The file format, physical medium, or dimensions of the resource journalArticle 2021 Ansari MY Autophagy CCCP Chondrocytes Confocal microscopy Department of Anatomy & Neurobiology Haqqi TM IL-1β immunofluorescence Inflammation January 2021 List journalArticle Methods in Molecular Biology Mitochondria Mitochondrial dysfunction mitophagy MitoTracker Deep Red NEOMED College of Medicine Osteoarthritis Oxidative Stress Parkin PINK1 Text A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text. URL Address <a href="https://link.springer.com/protocol/10.1007/978-1-0716-1119-7_15">https://doi.org/10.1007/978-1-0716-1119-7_15</a> ISSN 978-1-0716-1119-7 NEOMED College NEOMED College of Medicine NEOMED Department Department of Anatomy & Neurobiology Update Year & Number Jan to Aug list 2021 Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Assessing chondrocyte status by immunofluorescence-mediated localization of parkin relative to mitochondria. Creator An entity primarily responsible for making the resource Ansari MY; Haqqi TM Publisher An entity responsible for making the resource available Methods in Molecular Biology Date A point or period of time associated with an event in the lifecycle of the resource 2020 2020-12-15 Subject The topic of the resource Immunofluorescence staining is a widely used and powerful tool for the visualization and colocalization of two or more proteins and/or cellular organelles. For colocalization studies in fixed cells, one target protein/organelle is immunostained and visualized by one fluorophore and the other target protein/organelle is immunostained and visualized by a different fluorophore whose excitation emission spectra does not overlap with the first fluorophore. Parkin (PARK2) is an E3 ubiquitin ligase which performs ubiquitination of surface proteins of dysfunctional mitochondria to mark them for autolysosomal degradation. Here we describe the immunofluorescence staining of parkin protein and immunofluorescence or dye-based methods to visualize mitochondria and study the colocalization of parkin and mitochondria in primary human or mouse chondrocytes or cell lines. Identifier An unambiguous reference to the resource within a given context <a href="https://link.springer.com/protocol/10.1007/978-1-0716-1119-7_15">https://doi.org/10.1007/978-1-0716-1119-7_15</a> Rights Information about rights held in and over the resource © Springer Science+Business Media, LLC, part of Springer Nature 2021 Format The file format, physical medium, or dimensions of the resource Journal Article 2020 Autophagy CCCP Chondrocytes Confocal microscopy IL-1β immunofluorescence Inflammation Mitochondria Mitochondrial dysfunction mitophagy MitoTracker Deep Red Osteoarthritis Oxidative Stress Parkin PINK1